2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

This are slowly but the color and the leaves seen to be sativa and nice size of the leave. This time i put some top crop veg to estimulate the growing, i hope you enjoy the little videos.

DAY 49: seeing harvests from other grows of these strains, I wonder why am still going strong on the bud building ?. They are more than happy to carry on further at this point it seems with only a small covering of browning pistils on the FFT#1 showing out. She isn't the fattest buddies yet but hopefully this week will finish her off nicely. FFT#5 is very nice looking now and has so.e.beautiful sized colas across her wide span. She is very hemmed in by the others around her but has easily managed to push some serious buds to the canopy top to create so e rock solid, sweet smelling buds. She is feeding g a hell of a pot on the water now with approx 10L per 2 days. Hungry girl. FFT#7 Is still the biggest sister here and now with very nice packed out colas at every point I look. I will be very surprised if she isn't the highest yield of the whole run. It is a great strain to work with and watch grow. The smell off her as with the others is a fruity hit and rushes out of the tent when i open up. I think another week at least to find her sweet spot for harvest.

Récolte de 70 grammes sec pour une plante en pot de 7 litres, magnifique. Royal Critical auto à refaire

Day 57 😁👌all the queens are ok 🙏

Gave water at 6.5ph will feed on week 3. I think I've had the light to close. On day 10 pictures you can see the leaves look like there burnt 🤔 or that's what I'm guessing it is. I've raised the light to 1meter it's only at around half power at the moment and showing 21000 lux.

So... the Preflowering is real... we've got immens Plants which just want to Flower. I'm totally impressed about this Green Gold at Outdoor Conditions. - Greetings your organically » tried and true, since simple is the best!

É stato bellissimo, Pamela era davvero molto bella. Coltivare FastBuds è sempre certezza di gioia. Pamela è cresciuta di un ambiente ostile con temperature comprese tra 7 e 15°C, questa condizione climatica ho avuto la sensazione che abbia solo rallentato di una settimana la maturazione. É cresciuta senza una grande luce (led 100w), è stata curata pochissimo e per lei nessun miglioratore di rendimento LST. Nonostante questo ha prodotto un raccolto superiore ai 70g di qualità eccelsa e con un fascino unico tipico delle Purple. Gnam al gusto.. relax

22/11/2022 Se nota gran cantidad de pistilos en la planta un poco de estiramiento final 23/11/2022 Formación final de pistilos. Los cogollos comienzan a crecer considerablemente 25/11/2022 😰 Continua el crecimiento de cogollos. 26/11/2022 Cogollos comienzan a engordar

Elle ont été transplantés en début de semaine avec promix conect. Wow tout un effet . Elle ont doubler de grandeur . Les odeur son vraiment fort pour des plante qui ne sont pas encore en fleuraison . Très skunky et fleural. Encore quelque semaine à se faire travailler avant le switch en 12/12

She's growing strong in her first week. I think she'll be ready for topping in her second week. I'm keeping the humidity high to compensate for the high temperature during this sensitive seedling phase. To give her the best start and prepare her for topping, I'm also balancing her water acidity to about 6.5.

Risultato finale inaspettato! Questa è la prima volta in outdoor, e pensare che le volevo regalare perché non me la sentivo di farle crescere a casa perché troppi vicini di casa, poi mi sono affezionato ed eccole qua, le più grandi piante mai riuscite!! Sono molto contento!!

All in flush now except Acapulco Gold. The CBD Blue Shark and the Peyote Critical will hopefully be coming down by the end of next week. The Gorilla Zkittles in about 10 days or so.

Videos were taken on day 37 of flower. The mac x peanut butter breath plants are looking great ones in the back left and ones in the back middle towards the right side. They have more orange pistils at this point than all the other plants and are packing on the frost. I gave all the plants just plain water with herculean harvest, enzymes and humic acids last feed and flushed it a little but and it seemed to respond well. The rocstar breaths are frosty too and smell gassy. Also, I just checked some of my DMs on here and theres alot that I've never responded to, I dont check them unless it's from a business. I put in a few diaries a while ago if anyone has any questions for me to ask them in the comment section or a journal that way people with the same or similar questions can see it and I dont have to answer the same thing 8 times. I'm happy to answer any questions about my grows and shit just try to ask them in the comments✌️

3 weeks :) The girl looks healthy and happy, now I'm looking forward to her rapid growth and she will get bigger :) Everything is going smoothly :) it will be an interesting experience, because this time I probably won't use LST at all, I haven't tried letting her grow naturally :) good luck to everyone

Explosive growth this week, specially R. Cheese #1 and N. Lights #1. But both #2’s are also growing strongly and looking great. Mixed a 15L solution at full strength, will be looking for any deficiencies in the next few days.

Bem minha menina vai ficar, mais 12 semanas +/- em vegetação, Tenho minha sala de floração cheia. Bem arrumadinho dava, mas gosto que plantas tenham espaço para crecer. Até é bom, ramos laterais tem tempo para crecer, Deve é ter que cortar, cruto principal para não ficar muito grande, em comparação com resto da planta, ver como corre. Bons fumos Plantas felizes

Questa e la terza settimana e le ha subito 3 topping avendo così 8 rami apicali .....mi piace tantissimo e si è ripresa veramente bene pur essendo una autofiorente...ho scelto di usare i fertilizzanti BAC che mi sono arrivati direttamente dalla Spagna grazie al mio amico frits che ringrazio infinitamente per avermi dato fiducia per testare i suoi prodotti vincitori di numerosi premi! Da molto tempo che volevo usarli e sono rimasto veramente sorpreso , semplici e concentrati ! Ottimo anche le bottigliette molto molto resistenti...se ci sono delle bocce deformate e per via del prodotto pregato all interno 💪🏼....questa varietà di fast buds penso che sarà una delle mie preferite! Ho questo senso senso...al momento lascio stare tutto come sta e la lascierò rimprendersi per bene! E la farò entrare in fioritura così! Ringrazio anche Enzo e tutto lo staff di FAST BUDS per questa splendida genetica che mi hanno dato sotto stretto consiglio !!!! È una roccia! Staremo a vedere le prossime settimane! Nel frattempo sono felicissimo di tutto grazie anche a tutto il pubblico di growdiaries che mi dà la carica di continuare a fare tutto questo! Grazie mille a tutti ragazzi!!!!

Three of the four original seeds have sprouted and are growing well. I planted the fifth and last seed as an replacement.