Slight ph

January 2-8 01/02 Lots of growth in the two days since the last tea Topped the biggest one 01/03 Topped the other two and watered

Commencing week 3 - Day 15 on December 15th Change has taken place. Switched out COBs for SolarStorm 440. Currently on VEG mode. BS is a freak. Still has 4 cotyledons and now is displaying Tri-whorled mutation 🤔😄👍 Thanks for your time and attention!

Soon to feed flowering nutes after only 3 times feeding the nitrogen with some other stuff in the grow nutrients.. Soon to connect carbon filter as i disconnect it until the girls start to release strong smell.. I tuck leaves everyday and try my best to end up having even cannopy.

Kept ppm around 550-750 and waterline at 9’. Last defoliation on day 18 of flower.

Everything good except a bit of salt buildup caused by a poor runoff over the last weeks. You can see this by looking at almost every tip of the leaves. Last week Amazon had some problems delivering new ferts, so from day 97 to 101 I gave just 1ml/L of Bud Factor X and no Bud Candy and Sensizym at all. On day 101 I made a new nutes mix, and it’s the one you can see on GD. No more Bud Factor X because it’s very expensive and I don’t think I’ll need it for just two more weeks. I used 1ml/L of Cal Mag Xtra because I ended my Connoisseur Bloom Part A too. Lol, it’s been a problem. I ordered some Sensi Bloom Coco A+B and another Bud Candy for the last two weeks by the way. Flowers are now enlarging since the first day I added Overdrive. Very very good, I like this Silver Haze.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Catching up to the Alaskan purple now very bushy have had to tuck and trim quite a few leaves this week by the next morning it’s always twice as full started getting buds this week just the first hairs but right on track the bud blood and igniter work well for helping in transition 2 feeds per week now

Not much happened this week, but they are stretching. 😄

Our beauty is now 9 weeks old and is in full bloom, delighting us with her appearance and aroma. Her buds are getting covered in a thick layer of fragrant trichomes, and the air is filled with a pleasant fruity scent—promising a wonderful finish. The climate in the tent remains stable: daytime temperature is 28°C, nighttime drops to 21°C, and humidity is kept at 65%. The light schedule is still 12/12, and we continue using CO2 to support the flowering process. We’re also consistently feeding her with Xpert Nutrients, which provide everything she needs during this critical stage. A huge thank you to Xpert Nutrients for their top-quality fertilizers that drive such vigorous flowering, and to Seeds Mafia for the amazing genetics that gave us this strong and aromatic beauty. We’re eagerly looking forward to the moment she fully ripens and rewards us with a great harvest!

ladies and gentlemen .... welcome to the end of this other journey together in the enchanted valley of Peakyplanters 🌈 My God .... the plant in front is really impressive 😲 it literally branched its flower around the net and a huge cactus came out of it !!! it was a real shame not to be able to balance the nutrition of the other two, this is the proof that each plant has its own diet! So, in a way, it lacks a little structural strength ... but all in all it wasn't that bad ... I only suffered a bit in the final weight Its flowers have a very particular smell 😋

Pheno 1: strong natural tart cherries with a slight candy Pheno 2: loud cherry candy Pheno 3: cherry fun dip Pheno 4: cherries mashed with earthiness Pheno 5: the strongest cherry pheno I’ve ever smelt. Just loud cherries in your face. I kept pheno 3 and 5. I pressed both into rosin and the terps were insane it smelt like I worked a double at the marischino cherry factory. The terps on 3 were cherries on the inhale with a candy fun dip exhale (absolutely up my alley). Pheno 5 was the most in your face cherry I’ve ever tasted. It was straight cherries throughout the inhale exhale with a slight tart/candy on the back end. I have found my cherry phenos for my future business. Thank you for riding this journey with me. Harvest results on my YouTube channel link in my bio!

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 22 since the time was changed to 12/12 h. Hi everyone :-) . This week she has had extreme growth :-). In the next 5-10 days she gets 1 g GHSC Powder Feeding to 1 l coco. It is poured every 2 days with 1.2 l per pouring. Otherwise everything was cleaned and refilled. Have fun with the update stay healthy . You can buy this Strain at : www.Zamnesia.com Type: Runtz ☝️🏼 Genetics: Zkittlez x Gelato 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205 W 💡💡☝️🏼 Soil : Bio Bizz Coco ☝️🏼 Nutrients : Green House Seeds Company Powder Feeding Bio ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8

Day 47 and I switched ferts completely. I wanted to find something besides 20 additives so I'm trying jack's. Old timers know what they are talking about and swear by this stuff. They are responding great to it already in 2 days!! So easy too. I also changed bulbs from a well known brand to a denkyu bulb. This bulb is awesome and is way brighter. Not sure why neither one of my blackberry have turned any color.

Welcome to the Sweet Seeds Cup 🏆 Hi everyone 👋 This week she continued to develop really well 😍. From this week on she will receive 3 ml of Canna Bio Flores. She starts to put the whole production into the buds :-) Next week she gets 4 ml per L :-) Otherwise there is not much to report this week :-). I wish you all a nice start into the new week, stay healthy 🙏🏻 and let it grow 🌱👍 The variety San Fernando Lemon Kush is available here at Sweet Seeds 🌱 https://sweetseeds.es/de/# ☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼👍 Sweet Seeds Cup 🏆 Type: San Fernando Lemon Kush ☝️🏼 Genetics: OG Kush x Kosher Kush 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Earth: Canna Bio ☝️🏼 Fertilizer: Canna Bio ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 6.0 - 6.3 💦💧

My grow room is a mixture between a SOG and SGROG; they are packed in tightly and any branches that reach up to the light are being bent over and held in place by wire. Today I've added tomorite (half strength) to all the plants. they are still not flowering but there are loads of preflower wispy antenna at every node. The first plants are Amnesia Haze and the photo is of the two plants, the furthest one away has the broken main stem

Bonjour à tous les padawans et maîtres jedis Rien à faire cette plante pousse bien toute seule je lui donne de l'amour et Hesi Elle sent très bon et m'offre des futures têtes énormes Les trichomes sont bien laiteux je commence le rinçage la semaine prochaine

AAAnd there you have it. A nice experience, my second time making seeds! I harvested the first day of week 15. A lot to say about this experiment. It came out awesome, lots of seeds (more than 100!) Wich I also tested and came out with the exact same phenotype. I added some more pictures of the outcome and of the seeds, cause I don't have much more data about the harvest! Sorry for the last time haha! Also! Leave a comment if you want to see more old diaries, I might have 4 or so documented like this, Including the first test of the seeds Wich you see some pics here 🤞.

I’m so excited to show you guys week 3. These plants are huge!!! Even though I’m watching them grow right before my eyes I’m still shock as to how big they are. I browsed through other growers diaries that’s growing the same strains as me and I haven’t came across none as big as mines. The plants are responding well to their nutrients and TLC from me. I was planning to scrog this grow but I’m curious to see how big these autos are going to get so I’m going to just let them ride it out. Make sure to follow my diary for your weekly updates and give a like if you like the grow. Peace Love & Guidance #OwlGang #AGrowingEmpire