1/8 prepare Easy combo bloom: hello then this day I prepared the easy combo bloom of the royal in 6 liters of water with controlled ph, this nutrients make the flowers become big, among other things this will be like a booster! on i bud 30/07/2019 I can say that this strain drinks! in fact, he drinks a lot and the nutrients don't seem to bother even at higher doses ... has anyone noticed the same? 17/06 if you have seen the diary from the beginning you have understood that the counting of the weeks starts when I put the seed to sprout ....

Sin duda son las que llevan la delantera en mi pequeño armario, ya estan en su maceta definitiva de 11 litos y con la poda Apical, ahora a esperar que crezca un poco mas para cambiar el horario de luz

Thoses ladies are smelling strong. One is about 2 week late on the other, i think he got different phenotype, and quite sure that the late one will have a bigger harvest. They are healthy and strong. I'll start to wash the first one in something like a week. Everything is going well 😎

White LSD Auto Feminized Seeds by MSNL 👉Sponsored Grow👈 W7F6 8/4 10-16 Watered at the end of last week and have not watered yet this week. I have been foliar feeding at least once a day with the following blend: Calmag 15ml Plant Juice 60ml GH bloom 5ml Green Sensation 4ml Feed was ph 6.37, ppm 784 at 65f. I ph’d the water down to 6.25 with brought the runoff down to a range of 6.7-7.42 on the plants in the tent. I hope this helps my tent family recover from lockout, but it doesn’t look like it to me. White pushing out more hairs in the buds. Thank you all for your continued support and encouragement. Your likes and comments motivate me to keep sharing my journey. Let’s continue to learn to grow together! Stay green, growers love 💚🌿 💫Natrona💫

Good. Simple.

Our Permanent Marker by Zamnesia is a beauty that has started upwards and is not stopping anymore. ** Remember that we are growing a plant worked with the techniques indicated and another left to grow without pruning to preserve its speed; in the previous/next diary you will find the other plant of the same variety. Here the plant has let itself go straight and free and is thanking us very well by giving us some very very beautiful flowers. We are in a 100% organic configuration, the soil is recycled Promix + 1/3 fresh soil + 10% Perlite + RQS Mycorrhizae Mix (4 g in the mix, 1 g under the small fiber pot). We switched to the advanced flowering program a couple of days ago but last week we still did not give green sensation. We fertilize with: // 1 ml/l Sugar Royal - 3 ml/l Alga Bloom - 1 ml/l Power Buds - 1ml/l Green Sensation We sprayed 5 ml/l Vita Race Foliare once a week. For all the info https://plagron.com/ All nutrients are available from Zamnesia: https://www.zamnesia.io/it/531-growshop/476-fertilizers We always have the excellent mycorrhizae from RQS that run through the soil. https://www.zamnesia.io/5778-mix-micorrhiza-easy-roots.html Try this variety, it is a top strain of recent years very high level of thc ---- // https://www.zamnesia.io/it/12508-zamnesia-seeds-permanent-marker.html Brief description of Zamnesia // Here is a good opportunity to enrich your genetic library with another award-winning variety: Permanent Marker was crowned Strain of the Year 2023 by Leafly, thanks to its intense aromas, high levels of THC and its physical vivacity. Originally created by JBeezy and Seed Junky Genetics, our team managed to secure a cutting of this modern top cultivar, so you can grow it comfortably at home, whether indoors, outdoors or in a greenhouse. Permanent Marker fits perfectly in any environment! The whole world of growing and more is at Zamnesia: just take a look at the site and you will find "all the best that nature has to offer" in various shapes and colors. -- // www.zamnesia.com

Hey everyone :-). There is not much to tell this week :) A few came to the flowering tent, 2 are still in the vegi phase for 2 days and then come last to the flowering tent :-) The Blue Cheese and the Kosher Tangie Kush smell very good and how they should 😍👍 It will be difficult to choose 2 mums :-). I wish everyone a nice week 👌 Let it grow

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Buds are completely exploding

Day 21 - Ladies are looking nice & healthy! Everything seems to be going great, one is still growing a little slow but all in all extremely happy with this grow so far! Thanks for following & happy growing friends!✌️🏼🌱 Day 22 - They are really starting to take off now😍😍 Day 23 - not sure if it’s the pheno type or the nutrient but the plant that is getting the Technaflora Nutrients is starting to get a little bigger🤷‍♂️🏼 Day 24 - Babies are really starting to take off😍😍 got my New420GuySeed gear in today as well👍🏼🙏🏼 Day 25 - Starting to really grow now, the smaller of the 3 I starting mainlining today to do another comparison at harvest time! Day 26 - Another good day of growth! Thank you for following & happy growing friends!🌱✌️🏼 Day 27 - End of the week & these ladies just keep looking better & better😍

And this girl is on fire and in flower mode, i mean in just a few days she wen from big girl to a retarded enormous one, and she is not stoping at all, and i kind beed her to stop growing , she is way to tall for and indoor closet auto plant , go and figure this one out , i have no idea whats happening but they all are getting massive beyond expectations ever imagined , not complaining at all, just saying , i mean look at the size on this freaking monster, and at the same time look how gorgeous she looks , at least yo me she looks sexy af hihihi and now that she is in flower i can start spanking her , staring by defoliate the hell out of this cutie at some point very soon <3 <3 <3 Lets see how all goes She keeps going and going and dont want to stop, need to defoliate asap OMG Not STOPING and not so much time to defoliate yet …. I will i promise, i must , space is kind a getting in an SOS situation … OMG lol <3 <3 <3 As always thank you all for stoping by, for the love you guys put in the community, in to growing and it all, i am blessed to walk among all of you and am blessed with it all. Genetics : AUTO SOMANGO GLUE @ ADVANCED SEEDS All info and full product details can be find in can find @ https://aptus-holland.com/ https://advancedseeds.com/ https://autopot.co.uk/ https://lumatek-lighting.com/ #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #growerslove #dogdoctoofficial With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing in return and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 More info and updates @ https://growdiaries.com/grower/dogdoctor https://instagram.com/dogdoctorofficial https://youtube.com/channel/UCR7ta4DKLFMg2xxTMr2cpIg <3 <3 <3 Growers love to you all <3 <3 <3

Here we are in week six of flower and not a lot has happen this week other than the usual watering and letting her do her thing. Only trouble this week was updating GoPro had to try a few times. Other than that we got some rain in my home town and collected about 60 gallons that I'm going to use to finish the rest of the grow. Also, got my new AC Infinity CLOUDLINE T4, Quiet Inline Duct Fan System with Temperature and Humidity Controller, 4-Inch. I was really hoping this two plants would finish before the year ended, however, it looks like they got others plans. See you guys next week.

Green House Feeded one is enjoying life and growing big health buds. She received her last lollipoping. Advanced Nutrients one is still struggling. I am increasing the feeding per Advanced Nutrients supports suggestion. Let’s hope she recovers.

💩Holy Crap We Are Back At It And Loving It💩 👉WE R SO CLOSE NOW👈 Growmies we are at DAY 77 and there all just killing💀it👌 The Gorilla Punch Is Amazing 👏 An has been chopped 👈 👉We will be chopping the OG Kush this weekend 👍the Afghan kush still requires more time 👈 So Shit , I gave them just a tad to much nutes at the start feeding 👈 But I have since fixed it So I'm still doing some low stress training 🙃 and some defolation 😳 Lights being readjusted and chart updated .........👍rain water to be used entire growth👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

Giorno 1 bene le ragazze si sono riprese bene dal trapianto e stanno sviluppando bene le radici e iniziano a bere più acqua. Al termine di questa settimana verrà pulita bene Giorno 3 LST Giorno 4 leggera defogliazione sotto, annaffiata con 1L di acqua Giorno 7 defogliazione e lollipopping

Week 1 flower (Week 6 total) Got some calmag deficiencies as i can diagnose(im a fkin noob) but they were in 0.4 ec for days after beeing high for a while 1.45 EC of 1mll Calmag 1.5mll Micro 1mll Grow 1.5mll Bloom 0.5mll Uptake

Hatte leider 5 tage massive hitze probleme mit viiiel zuwenig luftfeuchte. Aber 2 tage mit richtige werte und sie schlagen zurück. Alles weeder gut und jetzt schick ich sie lieber mal in den 12/12 modus. Da sie sehr zum spargeln neigen. Freu mich schon auf die reise mit diesen schönen ladys👌😇 Die besten und saubersten clone gibts wie immer bei ROOTS-FARMS. best in austria and maybe in europe!💚👌🌱