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Heute wurden die Mädels getoppt und alles haben es sehr gut verkraftet
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@cRypto666
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Freunde der Sonne :) KURZE INFO DER LETZTE EINTRAG HIER IST IMMER DER AKTUELLLSTE NACH DATUM NACH UNTEN SOLLTEN UNTEN SCROLLEN UM ZU VORHIERIGEN EINTR'GE ZU GELANGEN : <3 WEEED wollte dir ein text drücken :) hab mich vertippt... aö ich bin heute eine Bewustseinsstufe beim Grown aufgestiegen ! Endlich kann man jetzt Täglich meine Real Talk oder Reaktion ViD/PIC anschauen. Absofort werde ich meinen GARTEN anderts wahr nehme wer rein schaut kann Exklusive Tipps für seinen Garten gebrauchen. Gerne bin ich für Fragen offen und ich helfe wenn ich kann fals nicht findet sich in der Gruppe immer jemand. DER GRUND IST DAMIT ICH NACHVOLLZIEHEN KANN WAS ICH FALSCH GEMACHT HABE BZW UM WISSEN ZU MANIFESTIRE UND GEWISSE GEDANKENVERBINDUNGEN GESACHFFEN SIND. IM GRUNDE MEIN GROWTAGBUCH ICH TEILE MEINE GEDANKEN MIT EUCH GLEICHGESINNTEN UND WÜNSCHE ALLEN EINE ERFOLGREICHEN GROW brO cRypto666 2 minutes ago cRypto666 <LIEBE> BRUDER ICH LADE DIE VIDEOS UND BILDER NOCH HOCH UND ICH BIN MIR EIGENTLICH SICHER ES KÖNNTE DIR GEFALLEN VIEL SPAß AUF QEEN SEED GIBTS WIRKLICH GUTE BLOGS MIT TOURTORIALS FÜR NEULINGE/ANFÄNGER . Konnte mir so mein Verständnis und Grundlage die fürs grown wichtig ist gut lernen und Aneignen ! WIRKLICH VIELE UND GUTE INFOS !! https://www.royalqueenseeds.de/blog-cannabis-anbau-c3 https://www.royalqueenseeds.de/blog-die-10-grossten-fehler-von-cannabiszuchtern-n86 es ist soweit heute beginnt Woche 1 ... BEVOR WIR STARTEN, WICHTIG WASCH DIR GRÜNDLICH DIE HÄNDE UND BENUTZE GUMMIHANDSCHUE BEIM ARBEITEN! SEHR WICHTIG! Ich freue mich das ich heute wieder das Gärtnern begonnen habe und eine Hällfte der Samen in der Erde sind. Kennt ihr das Gefühl der Vollkomenheit wenn man sich im Garten beschäftigt ? Für mich ist das Therapie und für euch? Ich spiele mit dem Gedanken anstatt Samen zu growen mir einfach eine Mutterpflanze Anschaffe um mir meine Stecklinge zu ziehen ! Dafür brauch ich aber noch ein anderes Zelt und eine Lampe. Die Mütterpflanze kann dann erst mal wachsen und ich kann nebenbei noch mal ein Durchgang mit Autos schaffen bevor die Steckis fertig sind. Wenn es soweit ist gebe ich euch bescheid wenn ich Samen und Setup bei mir stehen. So jetzt aufs eigentliche zurück. Ich habe heute erst mal mein Setup geprüft und so gut wie möglich desinfiziert. Es ist verdammt wichtig das nach jedem Durchgang sauber gemacht wird also zb Zeltwände, Boden, Topfe, Boxen, Wasserbehälter. Habe ich gemacht und habe meine ersten Samen in Anzucht Erde gesetzt, befeuchtet, markieren und in die Box. Wände und Deckel mit zersteuber befeuchten und ab unter die Lampe. WICHTIG MACH DIE ERDE NICHT SO NASS ! DAS GEFÄLLT IHNEN NICHT SONDERN HALTE SIE AM BESTEN FEUCHT IN DEM DU ABEND / MORGEN EIN BISCHEN WÄSSERST! ICH STELLE DIE LAMPE AUF CA 40 cm zum Boden ein bzw der Box. Ein gewisser abstand muss sein um die Fläche komplett zu Beleuchten. Da meine Lampe dimmbar ist muss sie nicht ganz oben hängeng sondern Kann näher an die Pflanzen ran und Stelle den Dimmer auf 20 %. Das reicht anfangs in dem Stadium völlig aus, muss aber dann später angepasst bzw hoch geschraubt werden! Lüfter I läuft 24/7 und sorgt für optimale Bedinungen! WICHTIG LAMPE 20/4 HELL/DUNKEL (REGLER STEHT AUF 20 % LICHTLEISTUNG) DANN HABEN WIR ES EIGENTLICH SCHON GESCHAFFT UND ALLES RICHTIG GEMACHT! Ich versuche schon so viel wie möglich von meiem Wissen an euch weiter zugeben bzw auf Papier zubringen, ist aber NICHT IMMER ZU 100% Möglich, da es einfach zu viele Informationen sind ! Bei fragen einfach fragen und die Comiunity hilft sicher !!! KOMMT ZEIT KOMMT RAT ... ich hab 2 Monate Infos eingeholt und Produkte verglichen ! Auf Queen Royal Seeds gibt es gute Anfänger Tipps sogar teilweise mit Video. Mir hat das sehr geholfen mein Grundwissen aufzubauen. BEVOR IHR DAS GÄRTNERN ANFANGEN KÖNNTE MACHT DIR GEDANKEN WO DEINEN GARTEN STEHEN KANN. #1 AM BESTEN IHR HABT EIN GARTENZIMMER DER NUR ZUM GÄRTNERN BENUTZT WIRD IST DIE BESTE LÖSUNG DA DU DEINE UMGEBUNG VIEL LEICHTER SAUBERHALTEN KANNST #2 DU KANNST DEIN GARTEN AUCH INS WOHN. BZW SCHLAFZIMMER STELLEN WENN DU KEINE MÖGLICHKEIT HAST EIN GARTENZIMMER ZU MACHEN. DAS IST NICHT DIE PERFEKTE UMGEBUNG ABER WENN DU ABLUFT RAUSLEITEN KANNST DANN MACH ES ! DU KANNST ES AUCH IN DEN RAUM STRÖMEN LASSEN MUSST DIR ABER BEWUST SEIN DAS ES EVTL DAS SCHIMMELN IM RAUM ANFÄNGT! IST MIR SCHON PASSIERT ! LEITE SIE WENN MÖGLICH AM BESTEN RAUS ! ACHTE DAS DARUF DAS DEINE LUFTFEUCHTIGKEIT UND TEMP KONSTANT BLEIBEN UND ES NICHT EINMAL ZU WARM UND EINEMAL ZU KALT... IHR WISST SCHON SORGE FÜR EINEN KONSTANTE UMGEBUNG IN DEINEM GARTEN. DIE WAHL DER SAMEN IST AUCH WICHTIG DA ES SORTEN GIBT DIE EINE WARME UMGEBUNG EINE KONSTANTE ODER EINE EHER KALTE UMGEBUNG. AM BESTEN IHR PASST EURE SAMEN EURER UMGEBUNG AN HEIST DU WOHNST IN EINEN LAND WO ES EHER WARM IST ALSO 25-30 ° C dann WÄHLE AUCH EINE PASSENDE SORTE AUS! MEHR GIBTS ERST MAL NICHT ! BIS ZUM NÄCHSTEN MAL!
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Day 26 - LST and light defoliation. Day 28 - Tucked the leaves, and noticed some perspiration between where two leaves were laying against each other. 1 gallon dosage of the above listed nutrients should last about 5 days before drying out, to be ready for the next dose.
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This is the end of the 6th week. (Thats a little japanese bonsai in with it) Feeling like it isnt coming along as well as it should be.. Its fanning out like mad, but not growing up, stayed the same height for the week. I shifted it into a pot that wasnt clear as suggested and topped it and snipped a few other buts underneath off. Also my light arrived which is currently on 80% and at height of 450mm/45cm/17.7 inches My nutrients n shit haven't arrived yet, been waiting on those for a week thanks to a postal strike. So I had tiny barely visible bugs, which seem to have disappeared, not under leaves or crawling around anymore. Would they be what has caused my spots and drooping/clawing? Or is it simple because of my light, lack of food, too much water? Very much need some help with this.
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2018.12.07 - Clone Day 68 - Flowering Day 16 I'm struggling with how to address the significant difference in height between #5 and the rest of them. I much prefer growing from seeds, where I know how to top, manifold, and LST to create an even canopy layer.
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@Sadhus
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**Semaine Harvest : Récolte Finale – Sticky Broccoli (28 avril 2026)** **Note :** Fin du cycle Eternity Grow Cup 2. Récolte effectuée en semaine 10 de floraison (légèrement anticipée). Tout le journal de culture est maintenant complet. ### Contexte et Bilan du Cycle Après 10 semaines de floraison (semaine 14 globale), la récolte a été déclenchée suite à un départ de botrytis sur une autre plante du même espace. J’aurais pu laisser encore quelques jours pour laisser mûrir un peu plus les trichomes ambrés, mais la sécurité prime. La plante dominante (150 cm) a donné un beau rendement qualitatif, avec des buds denses, extrêmement frosty et résineux. **Poids frais : environ 780 grammes** (poids humide total des buds trimés, sans les grosses tiges). Mise à jour prévue dans 2 semaines pour le **poids sec final** après un bon curing. ### Observations à la Récolte - **Buds** : Denses et compacts, très collants. Couverture trichomale exceptionnelle (majoritairement laiteux avec quelques têtes ambrées). L’aspect sugar est magnifique, les buds brillent littéralement. - **Odeur** : Très agréable, sucrée, épicée et florale, typique de Sticky Broccoli. Puissante même après le flush. - **Résine** : Extrêmement résineuse au toucher – parfait pour du dry sift ou un petit Ice-o-Lator. - **Structure** : Belle densité globale, même si la taille individuelle des buds reste dans la moyenne haute pour la variété (pas les plus gros que j’ai vus, mais la qualité trichomale compense largement). - **Santé** : Plantes propres jusqu’à la fin, pas de carence visible. Le flush des 3 derniers jours (EC 0,7) a bien aidé à nettoyer les sels. **Clones** : Quelques clones prélevés plusieurs semaines auparavant ont été repiqués avec succès dans leur nouveau système. Ils sont bien enracinés et prêts à repartir pour un prochain cycle de végétation/floraison. La génétique est conservée ! ### Paramètres en Fin de Cycle (semaine 10) - Température jour/nuit : 19-21 °C / 14-15 °C - Humidité : 48-58 % jour / 29-39 % nuit - VPD : 0.9-1.3 kPa - PPFD : 550 (DLI 24) - UV : 90 min/jour - EC final : 1.0 puis flush à 0.7 (Terra Aquatica seul) - pH : 5.8-6.0 - Steering : génératif jusqu’à la fin - 48h de blackout avant récolte : appliqué pour booster la résine et améliorer la qualité des trichomes. ### Plans Post-Récolte - **Séchage** : 7-10 jours à 18-20 °C et 55-60 % d’humidité dans un espace sombre et ventilé. - **Curing** : Minimum 2-3 semaines en bocaux (burping régulier) pour affiner les terpènes et la douceur. Mise à jour dans 2 semaines avec le poids sec final. - **Utilisation** : Une partie en fleurs séchées/cure, et un petit batch pour **dry sift** + **Ice-o-Lator** (bubble hash) histoire de se régaler avec la résine exceptionnelle de cette Sticky Broccoli. **Tips éducatif final : Le flush et le blackout en fin de cycle** - **Flush** (3 derniers jours à EC très bas) : Permet d’éliminer les sels accumulés et améliore nettement le goût final (moins de chimie, plus de terpènes purs). - **48h de blackout** : Technique simple mais efficace qui stress légèrement la plante de manière positive, augmentant la production de résine et la clarté des trichomes juste avant la coupe. Ce fut un très beau cycle, propre et instructif malgré l’interruption due au botrytis. La qualité résineuse et l’odeur compensent largement. Merci à Zamnesia et Plagron pour le matos ! **#GrowLegendary #Zamnesia #Plagron #EternityGrowCup2 #StickyBroccoli** Dans 2 semaines je mettrai à jour avec le poids sec et les premières impressions après curing. **Estimation du poids sec final** Avec **780 grammes de poids frais** (buds humides, après trim initial mais avant séchage complet), voici une estimation réaliste pour cette Sticky Broccoli : ### Estimation raisonnable - **Poids sec attendu** : **180 à 230 grammes** (environ **23-30 %** du poids frais). **Pourquoi cette fourchette ?** - Les buds frais contiennent typiquement 70-80 % d’eau. - Après un bon séchage (jusqu’à 10-12 % d’humidité résiduelle) + curing, il reste généralement **20-30 %** du poids humide initial. - 20 % → **156 g** (estimation basse, si buds très denses ou séchage poussé) - 25 % → **195 g** (estimation moyenne, la plus courante pour des buds compacts comme les tiens) - 30 % → **234 g** (estimation haute, si les buds retiennent un peu plus d’humidité ou sont particulièrement résineux) Tes buds étant décrits comme **denses, très collants et frosty**, je pencherais plutôt vers le **milieu/haut de la fourchette** : **190-220 grammes** une fois bien secs et cured. ### Facteurs qui influenceront le résultat final - **Qualité du séchage** : Température 18-20 °C, humidité 50-60 %, bonne circulation d’air sans vent direct → perte d’eau progressive et uniforme. - **Trim** : Si tu as fait un wet trim assez serré, le poids sec sera un peu plus bas que si tu as laissé plus de sugar leaves. - **Densité des buds** : Plus ils sont compacts et résineux, moins ils perdent de poids relatif (mais ils sèchent parfois un peu plus lentement). - **Curing** : Pendant les 2-4 semaines en bocaux, tu perdras encore un peu d’humidité, mais le poids se stabilisera. ### Prochaines étapes 1. **Séchage** : 7-10 jours jusqu’à ce que les tiges cassent net (pas pliables). 2. **Curing** : Minimum 2 semaines (idéalement 4+) avec burping quotidien au début. 3. **Pesée finale** : Une fois le poids stabilisé (après 2 semaines de curing),
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KRITIC AUTO by KANNABIA Week #5 Overall June 23rd-30th Week #2 Flower This week she's doing well no issues with her feeding she's a good looking plant. Stay Growing!! Kannabia.com KRITIC AUTO
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[ Information ] For all grow information, including strain and room details, please see the first week of veg. [ Updates ] (Flower) Day 1 - Light intensity increased to 65%. C02 increased to 1300ppm average. Day temp/humidity 85/70 (1-1.2VPD), night temp/humidity 75/65 (.8-1VPD). Fed a diluted compost tea mixture before lights on. Base water was R/O and tap water mixed lightly with silica and Tribus microbes. Tea was a mixture of Fish Hydrolysate, Bat Guano, Molasses, Fulvic/Humic, Kelp, and Earthworm Castings. 8 gallons of tea were added to 72 gallons of water mixture for an 80gal batch total. The batch was mixed for a half hour before feeding to the room. I did not PH or PPM test the mixture, organic material is hard to get an accurate PPM reading so the numbers are useless to me. I will be working to bring the lights to 100% power over this first week of flower. Day 4 - Light intensity increased daily, currently at 90%. Will raise to full power tomorrow. C02 increased to 1800ppm average. Temp and humidity for day and night are still the same. Lights were raised slightly to maintain 12 inches from canopy height, and a few growth nodes that were above the canopy got topped. Watered today with an 80gal (1.25gal p/pot) mixture, 10% tap 90% r/o water. 6.8 PH, 2.2ec. Foliar sprayed yesterday before lights off with a neem mixture for weekly IPM. Canopies are stretching relatively evenly, though I will be adding in support nets within a few days to help maintain the even spread. I've run this strain before so I'm fairly confident that I know what to expect during these few stretch weeks. Day 7 - Lights have been at 100% since day 5. C02 still 1800-2000ppm on average. Plants are stretching quickly into the lights, I have yet to readjust their height. The best growth usually happens when I do nothing, and I've done almost nothing the past couple days besides enjoy the unusually warm spring weather my area is currently experiencing. Watered today, 100gal (1.5gal p/pot) mixture, <1ec. Mainly an organic feeding for microbe health, also wanted a bit more runoff than normal due to the high ec feeding previously.
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Resultado muy bueno, fue con la tabla completa de Advanced Nutrient y funcionó la maravilla, volveré a repetir las cepas, sacaron tonalidades preciosas.
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sto facendo una fine di ciclo pessimo...pochi risultati dovuti a vari errori... 1 non aver misurato mai ph di acqua(fatto pochi giorni fa era a 8) 2cattiva irrigazione(temperatura acqua soprai 28 gradi) 3 una un uso scorretto dei fertilizzanti mai misurato niente.. 4 arrivare ad agosto non e il massimo da me sto toccando punte di 30 gradi in su 5 stress termici sbalzi termici tempertura giorno e notte uguali 28/30 gradi non riesco a rinfrescare la stanza dove c'è grow
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@yan420
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FERMAKOR BARREL MIX – BASE IN USE (Testing on the Fantasy Feast girl we pulled out of another diary https://growdiaries.com/diaries/274722-grow-journal-by-yan402 ) (FERMAKOR BASE SYSTEM KOH VERSION diary https://growdiaries.com/diaries/278391-grow-journal-by-yan402) (Urea & Micros on the way — first week running without them) 🍶💧🍶💧🍶💧🍶💧🍶 💧 30 L Barrel – Current Working Mix 🍶💧🍶💧🍶💧🍶💧🍶 Step 1 – Calcium Nitrate (Part A) 7 L warm water (~35–40 °C) → added 45 g Calcinit, stirred until fully clear. That’s the calcium + nitrogen backbone for the feed. Step 2 – FERMAKOR PK Base (Part B) 15 L water in the main barrel → added 30 ml FERMAKOR PK Concentrate, mixed well. This forms the main P + K part of the formula. Step 3 – Combine Solutions Slowly poured the Calcinit mix into the barrel while stirring — no reaction, still crystal clear. That confirms the mix is stable and precipitation-free. 🌿 Step 4 – FPJ / FFJ Batch Added 30 ml homemade FPJ (fish + veg batch) ≈ 1 ml/L. Color shifted to a light-amber tone — looks alive and active. 🍋 Step 5 – Citric Acid Balance Added 1 tsp citric acid after everything was blended to fine-tune pH and help chelate micros later on. 📦 Step 6 – Top Up & Check Filled to the 30 L mark with plain water → pH tested with drops, showing yellow-green — roughly 5.8 – 6.0 range. Nice clean look, stable smell, no residue. 💧 Current Base Ingredients (Active Mix) Warm Water ≈ 22 L total Calcinit 45 g → N + Ca foundation FERMAKOR PK Base 30 ml → P + K support Citric Acid 1 tsp → Chelation + pH balance FPJ / Fish Emulsion 30 ml → Organic enzyme booster Result: clean amber mix, mild and balanced. I’ll let this version run for a week before adding anything. 👀👀👀👀👀👀 Observations and changes 👀👀👀👀👀👀 27.10.25 VW27 noticed some min burnt tips so I decreased Calcium Nitrate 45 to → 40g, decided to add two more elements micros and Epsom salts just to make sure they got everything, Fetrilon Combi 1 (Micros): 0.5 g, Epsom salts: 8 g 28.10.25 VW27 she seems devoid of any deficiencies, seems ready for the flip to 12/12 02.11.25 VW27 girl is looking good so I decided to stop making daily videos and do a standard once a week update. 09.11.25 aVW28 7 days since flip,stretch in full swing, first pistils showing, leaf color deep and healthy. Slight tip burn early week → gone after pH stabilized. Feed stayed clear, no residue, roots clean and sweet-smelling, did what I hope is a last cleanup and pruning🎥 10.11.25 VW29 added Phosphoric acid pH down to the schedule for flowering stage. 14.11.25 FW1 FERMAKOR PK Micros 40 → 50 ml 23.11.25 FW2 got some burnt tips, observe and act accordingly in case it worsens, diluted by 25% for this week. 05.12.25 FW3 about 2 weeks ago Calcium Nitrate 35 g → 25 g, FERMAKOR PK Micros 50 ml → 60 ml 12.12.25 FW4 Calcium Nitrate 25 g → 20 g, FERMAKOR PK Micros 60 ml → 80ml 16.12.25 FW4 Calcium Nitrate 20 g → 17 g, FERMAKOR PK Micros 80 ml → 110 ml 🌱💦🌱💦🌱💦🌱💦🌱💦🌱 🌿 Day to day tasks & actions 🌿 🌱💦🌱💦🌱💦🌱💦🌱💦🌱 Fed about 5l a day of #1 and on the weekends I do a pure FERMAKOR PK flush 2l runoff (*RUNOFF reused for tomato plants) 💧💧💧💧💧💧💧💧💧 🌱 Nutrients in 30 L #1 Veg – FERMAKOR 💧💧💧💧💧💧💧💧💧 Calcium Nitrate (Calcinit / Nitcal): 45 → 40 g → 35 g → 25 g → 20 g → 17 g = 1.50 g/L → 1.33 g/L → 1.17 g/L → 0.83 g/L → 0.67 g/L → 0.57 g/L = 207 ppm N / 253 ppm Ca → 184 / 225 → 161 / 197 → 115 / 141 → 92 / 113 → 78 / 96 PK Concentrate (FERMAKOR Base): 30 → 40 ml → 50 ml → 60 ml → 80 ml → 110 ml = 1.00 → 1.33 ml/L → 1.67 ml/L → 2.00 ml/L → 2.67 ml/L → 3.67 ml/L → balanced 1:1 P:K + light micros (from extract) Home-made FFJ/FPJ (Fish + Veg): 30 ml = 1.00 ml/L Epsom Salt (MgSO₄·7H₂O): 8 g = 0.27 g/L → ~26 ppm Mg + ~35 ppm S Fetrilon Combi 1 (Micros): 0.5 g = 0.017 g/L → Fe 0.7 ppm · Mn 0.7 ppm · Zn 0.3 ppm · Cu 0.3 ppm · B 0.1 ppm · Mo 0.02 ppm Phosphoric Acid (pH down) + Citric Acid (chelation): as needed → First set pH with phosphoric acid, then add a little citric only if you want extra chelation Target pH: 5.8 – 6.0 (drop test yellow-green) 📦 TOTAL: Liquids: 60 → 70 → 80 → 90 → 110 → 140 ml per 30 L = 2.00 → 2.33 → 2.67 → 3.00 → 3.67 → 4.67 ml/L Solids (CaNO₃ + Epsom + Fetrilon): 53.5 → 48.5 → 43.5 → 33.5 → 28.5 → 25.5 g per 30 L = 1.78 → 1.62 → 1.45 → 1.12 → 0.95 → 0.85 g/L YouTube Link: https://youtube.com/-m8h?si=A7x4Zlr2kj-_ga31
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@valiotoro
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A wonderful genetic thank you Fast Buds ❤️‍🔥🤩 The trim was super easy the buds are FAT & FROSTY full of resin 🤤 I’m very happy with the harvest 142g !! For the light i’ve used the Mars Hydro SP-6500💡
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2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.
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Still lookin healthy as she starts week 2 of flower. Middle branches are growing faster than the rest and I’m running out of room in the center to tuck branches in net.
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@Xabii
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Did a reservoir change. Found a bunch of seeds at the top buds, did not find any nanners or balls, most likely somewhere hidden in the bud or non at all and still seeds. The other plant in the same tent does not have a single seed on it. Values are average of the day. DATE - EC(us/cm) 20250528 2009 20250529 2030 20250530 2117 20250531 2125 20250601 2217 20250602 1396 20250603 1533 DATE - PH 20250528 6.13 20250529 6.13 20250530 6.15 20250531 6.15 20250601 6.17 20250602 6.16 20250603 5.92 DATE - ORP (mV) 20250528 174 20250529 163 20250530 142 20250531 131 20250601 108 20250602 151 20250603 116 DATE - °C - RH% (Tent Temp/RH) 20250528 24.8 54 20250529 24.7 51 20250530 25.7 55 20250531 24.3 60 20250601 26.4 55 20250602 24.3 48 20250603 25.2 46 DATE - °C (Reservoir) 20250528 21.1 20250529 20.8 20250530 22.0 20250531 21.5 20250601 22.5 20250602 19.9 20250603 21.9 DATE - CF 20250528 20.09 20250529 20.30 20250530 21.17 20250531 21.25 20250601 22.17 20250602 13.96 20250603 15.32
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C99 – Week 1: The Sativa Rocket Launches! 🍍🚀 Welcome to the solo-run in the mini-tent! While the high-end sponsor strains are taking over the big setup, this report is all about a legendary, fast-flowering classic: C99 (Cinderella 99) by Female Seeds! Running in my 40x40 cm tent, this project is designed to test how much punch, flavor, and efficiency you can pull out of a compact, single-plant Sativa setup under targeted lighting. The Setup for this Run: Tent: 40x40 cm (Solo-Tent) Lighting: Viparspectra XS1500 Pro (tuned to an efficient sweet spot) Medium: Coco-Perlite (50/50) Goal: A tightly trained, highly optimized single-plant setup focusing on structure, massive headbud development, and those iconic sweet pineapple terps. Current Status (Week 1): The seed is officially soaking in its pH-adjusted water bath, getting ready to crack. Once the taproot shows, it’ll move into its starter medium to kick off the journey in the small lab. Excited to run this absolute Sativa masterpiece alongside the big tent. Let's see how fast this rocket takes off! 🍍✨
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@SuperSuMo
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Growing up so fast
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Wo soll das hinführen ? Ich wünschte man könnte Gerüche festhalten.