Aroma has begun as the flowers are forming. Pulled alot of the large fan leaves to get light to the bud sites. Lots of tops from LST training so hoping she will be a good producer.

3 semana en flora ya mostrando varios brazos con flores así que contento con mi primera siembra, floración anticipada y tamaño adeudado para mi espacio..

Muchas gracias por dejarme participar BsF y grow diariaries y compartir este cultivo gorila glue 4. Fue un hecho en scroog en un metro veinte cuadrado usando dos masetas de 23 ltrs usanso microorganismos y productos orgánicos súper contento con esta sepa, fueron criadas con dos focos galoponeros en un armario con poco a lo q tengamos podemos hacer mucho lo importante es aprender un poco todos los días y disfrutar de nuestras cosechas un fuerte abrazo para todos @gorilaweed420

📆 Semana 7 La Lemon Cherry Runtz sigue su avance imparable en floración. Los cogollos se vuelven más densos y pesados, cubiertos de una capa de resina cada vez más brillante, que refleja la luz como un pequeño tesoro. Los pistilos comienzan a oscurecerse, anunciando la llegada de la madurez final, mientras el aroma dulce y afrutado se intensifica, envolviendo el espacio con notas golosas y envolventes. Las ramas laterales mantienen su fuerza, soportando sin problema el creciente peso de los cogollos, y la estructura general sigue equilibrada y aireada, perfecta para un desarrollo óptimo. Las hojas grandes ceden aún más espacio, dejando que la floración ocupe todo el protagonismo. 🌸 El espectáculo visual y aromático sigue en pleno apogeo, y lo mejor aún está por venir. Seguimos creciendo fuerte 💪!

OG 4Q24 Flower Week 10 Orangegasm (Fem) [ IRIE Genetics ] 12/12 @ Bolt (Day 21) Germination: 20 November 2024 #3A Earliest Harvest Date: 9 February 2025 #3B Latest Harvest Date: 19 February 2025 _________________________________________ Start of Week: [Wed Feb 12, 2024 CR2 4Q24 64:F:9:1] End of Week: [Tue Feb 18, 2024 CR2 4Q24 70:F:9:7] OrangeGasm Fertigation: - MAX: EC: [ 1.6, mS] - LightIntensity MAX: [ 850, µMol/m2/s] ______________________________________ __ Wed Feb 12, 2025 OG 4Q24 64:F:9:1 Refresh Reservoir - Amount: [ 5, gal] - EC: [ 1.6, mS] - Primer A&B: [ 48, ml] - SLF-100: [ 15, ml] Reclaim Dehu - Amount: [ 4, gal] EC: 1.6 Runoff - Amount: [ 1.25, gal ] - EC: [4.3 , mS/cm2] - EC∆: [ 1.6 , mS/cm2] # Danger!!! (I don’t think so - Happy Plants) __ Thu Feb 13, 2025 OG 4Q24 65:F:9:2 Runoff - Amount: [ 2, gal ] - EC: [ 4.6, mS/cm2] EC: 1.5 Refresh Reservoir - Amount: [ 2, gal] - EC: [ 1.5, mS] - Primer A&B: [ 32, ml] - SLF-100: [ 10, ml] Reclaim Dehu - Amount: [ 2, gal] If we see the pistils of the last two plants shrink/collapse in the next 3 days, we may be ready Tuesday/Wednesday . . . I also note we’re about 2 days after from OPTIMAL BUD Structure, Foxtailing is becoming less well formed at largest top and some of the sooner ready buds … __ Fri Feb 14, 2025 OG 4Q24 66:F:9:3 EC: 1.4 1.5 gal dehu 3 gals+ refresh Reduced Fertigation Time 50% as Tank is at refill after 4+ gal yesterday, again. :-{ __ Sat Feb 15, 2025 OG 4Q24 67:F:9:4 Reclaim Dehu - Amount: [ 2.5, gal] Refresh Reservoir - 2 Gal - Primer A&B: [ 17.9, ml] - Resin Bloom: [ 9, ml] EC: 1.3 Runoff - Amount: [ 0.5, gal] - EC: [ 3.0, mS/cm2] - EC∆: [ 1.7, mS/cm2] __ Sun Feb 16, 2025 OG 4Q24 68:F:9:5 Refresh Reservoir - 2 Gal - Primer A&B: [ 16.5, ml] - Resin Bloom: [ 8.3, ml] Runoff - Amount: [ , gal] - EC: [ , mS/cm2] - EC∆: [ , mS/cm2] EC: 1.2 __ Mon Feb 17, 2025 OG 4Q24 69:F:9:6 EC: 1.1 __ Tue Feb 18, 2025 OG 4Q24 70:H:9:7 1000 - Remove Plants from Tent - Remove Drip Tray - Remove Light - found water unseen in bottom tray - the reason for unreasonable humidity - Removed Drip Rings - Dried bottom drip tray - Resealed Tent - Low RH _______________________ Harvest Log Max Min Average Height (in) 40 21.5 29.875 Weight (g) 700 157 385.5 Secondary Stem Count 30 17 21.125 Est Dried Trimmed Wt 140 31.4 616.8

Bonjour les amis. Cette semaine le stretch a bien démarré et est bien sensible 😻. J'espère qu'il sera limité, mon placard est petit. Aucune odeur pour l'instant. La plus petite des 3 plantes fait 15 cm de haut. Les 2 autres font 29 cm et développent plusieurs têtes. Pour l'instant tout va bien. À bientôt 👋

GORILLA COOKIES 🍪 FF / FASTBUDS WEEK #17 OVERALL WEEK #9 FLOWER This week she's doing good buds are looking good they are mostly dense decent trichome coverage and she's got a nice aroma to her and her buds are looking good she's got a few big buds going on. Stay Growing!! Thank you for stopping by and taking a look it's much appreciated!! Thank you FASTBUDS!! FASTBUDS / GORILLA COOKIES 🍪 FF

Another week closer... The girls are doing well, nothing really new, besides the day we changed our clocks back I forgot to plug my control box back in and for all day they didn't get any water..didn't really do any damage but for one, where I got a little leaf dry out on a couple of leaves on one plant.. Besides that happening, everything else is going well. Until next week, smoke a fatty, help out your fellow grower.

Hello everyone 👋 Week 6 of flower for the Banana Purple Punch auto from Fast Buds 🍌😈 She grew fast with a beautiful color,for the nutrient 4ml/L terra bloom & 1ml/L power buds & Green sensation 1ml/L from Plagron one more time then only plain water 💧 Mars Hydro SP-6500 100% Have a nice day 😋

I recommend everyone try this strain, couldn’t be happier and it’s a freebie, I should have more photos coming soon, I’ll also update effects once I have smoked

26.02.2024 Clean and disinfect tanks and filters with h2o2+vinegar acid +bleach Mix new batch with 1.7ml AN connoisseur AB + Silica @ 1% H2o2 - total 30l Donne one pass off long misting 2m with diluted nutrient solution 0.7EC. Flush buckets with diluted solution PAR 350 PPFD, VPD 0,8, RH 60-62%, Temp Night-21c, Day-24/25c, Leaf temperature 22c, Light distance 75cm, CO2 650+ppm 340w@75cm distance 45s ON time 15min OFF time PT-Pressurized tank - PH- 5.9 , EC -1.05, Temp-21c NT-Nutrient Tank - PH -5.85, EC -1.0, Temp-19c 27.02.2024 Topping on all girls, 4 cut par plant Detailly clean grow room floor, benches and main room floor, From this week start feeding more, runoff is lower than input Lower humidity to 58-60% Girls looking great so far PAR 380 PPFD, VPD 0.9, RH 58-60%, Temp Night-21c, Day-25c, Leaf temperature 22c, Light distance 75cm, CO2 650+ ppm 360w@75cm distance NT-Nutrient Tank - PH -5.95, EC -0.9, Temp-19c 28.02.2024 Have timer malfunction, watering didn't work for some time, not affected by roots, still wet when realize defect Replace all filters, great condition so far, no need for changing After topping girls looking healthy, PAR 360 PPFD, VPD 0.9, RH 58-60%, Temp Night-21c, Day-25c, Leaf temperature 22c, Light distance 75cm, CO2 650+ ppm 380w@75cm distance NT-Nutrient Tank - PH -6.02, EC -0.84, Temp-19c Nutrient strength still drooping, next top-up will be a higher strength, around 1.1EC 29.02.2024 Check roots, they start to the bottom off the bucket, still nice and white Slowly continue to give more power to light, still have more than 1/3 off Diablo strength PAR 410 PPFD, VPD 0.9, RH 58-60%, Temp Night-21c, Day-25c, Leaf temperature 22c, Light distance 75cm, CO2 650+ ppm Nutrient Tank - PH -6.04, EC -0.81, Temp-20c Ph is stabile so far, i don't adjust anything at this point, lets swing a little bit. If start to going above 6.2 i will bring it down with PH- Humidifier operate less and less, girls make humidity in room 400w@70cm distance 01.03.2024 Again have issue with watering timers (DH48S-S), stop working again, noticed that early so no damage Will need to invest in better timer for misting, this one is start to malfunction😡 Girls growing fast this week, no problem at all😇 Every day their progress can be seen 😀 Like that girls are almost same height and structure, very uniform growth by any mean👏 Replace all filters and clean UV-c bulb PAR 460 PPFD, VPD 0.9, RH 58-60%, Temp Night-21c, Day-25/26c, Leaf temperature 22c, Light distance 70cm, CO2 650+ ppm Nutrient Tank - PH -6.05, EC -0.86, Temp-22c 414w@70com distance 02.03.2021 Girls start to drink noticeable more water and nutrients, EC drop in tanks by 0.2 in last two days, PH is stabile, going on base side by little Replace all filters , set Diablo to 440w without changing light height, let it shine 😀 Start to manipulate branches and tucking big fan leaves, recovery from topping is apparent, two new shoots are larger and larger every day If growth continue with this speed, flowering probably in middle off next week, probably don't want to wait more than that All three girls looking very similar, really great genetic , very uniform growth and structure Hot and humid outside condition, thin this year we hit several HIGH Temp Record! PAR 490 PPFD, VPD 0.9, RH 58-61%, Temp Night-21c, Day-25/26c, Leaf temperature 22c, Light distance 70cm, CO2 650+ ppm Nutrient Tank - PH -6.07, EC -0.80, Temp-22c 03.03.2024 Top up tank with 5l off fresh solution @ 1.2EC Nutrient strength still dropping significantly compared with starting , PH rising slightly. Need to adjust at 6.2 to 5.95 Next nutrient mix will be 2ml AN connoisseur AB @ 1.2EC Replace all filters, little participation on them, mostly from Kelp Remove couple off leaves from all girls, preparing for next week defoliation Forget to refill Humidifier so for couple off hours RH was 48-50%, girls drink more than usual, water and nutrients. Maximum PPFD at center is 600, at edges 440 PPFD PAR 560 PPFD, VPD 0.9, RH 58-61%, Temp Night-21c, Day-25/26c, Leaf temperature 22c, Light distance 70cm, CO2 850+ ppm Nutrient Tank - PH -5.95, EC -0.84, Temp-22c 480w@65cm distance

Grow cycle definitely does not correlate to manufacturer’s timeline, so be sure you plan accordingly. Although she’s not a bad plant doesn’t get too tall, odor isn’t too bad and she looks great!! After the cure I definitely love this strain wish she yielded a bit more but I’m happy with her. I definitely recommend this strain and any other Royal Queen Seeds strain.

It was a slow week, peeking at a fungul infection'

Hello Diary, Milky Way F1 is the most impressive of the three on the farm, at least for me. It is the tallest, 54 cm, with a beautifully pronounced main cola and branches filled with flowers. She is so beautiful. The leaves are of a healthy green color, the flowers are large and cover the entire ends of the branches. The smell has become more intense, which gives me special pleasure when I open the grow box. Watering has become more frequent, every three days each plant on the Farm receives about 2.5 liters of water. I continue adding Bio Flowering Booster and Easy Bloom tablets. Conditions on the farm are good. The temperature is around 28 degrees, while the humidity is around 45%. Here's what it looked like last week. 06/06/2023 - Day 38. Watering. I added all three components from the Bio Flowering Booster. pH I lowered it to 6.0. Each plant received about 2.5 liters of water. 09/06/2023 - Day 41. Watering. This time I put one Easy Bloom tablet in 7 liters of water and watered all three plants on the Farm with that amount. 10/06/2023 - Day 42. End of the sixth week. I photographed the plants and removed three or four yellow leaves. Milky Way F1 - 54 cm That's all for this week, thank you all for your support and see you soon.

Holaa, 6ta semana y ya vamos superando los problemas de temperatura. Recibí la manta calefactora y la temperatura con el led apagado subió a 16-17ºC, es un gran avance con respecto a los 13-14ºC que alcanzaba. Los esquejes van avanzando bien, el esqueje con la poda apical va desarrollando los tallos del centro y esta tomando una buena forma. El esqueje con LST esta tomando más fuerza en sus tallos principales y se vuelve más frondosa. Empece a regar agua con EC casi nula y no eh podido agregar los nutrientes básicos para llegar a un EC base de 0.4. Por esto es que creo que los esquejes están presentando una deficiencia de calcio para esto estoy a la espera del producto SENSI CAL-MAG XTRA de Advanced Nutrients con este voy a corregir la EC base del agua de riego y aplicare una fertilización foliar para las carencias presentes. Espero que los resultados sean los esperados. A los esquejes los acompaña la Negra 44 que tiene un diario aparte y un palto(aguacate). Cualquier recomendación sera bien recibida!!

I would definitely recommend the White Widow Max Auto to a beginner grower! I hope it tastes as good as it looks.

Bloom 42

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

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