Ladies are smelling strong and ready to be harvested. Chopping Venezia to be hung out to be dried tonight. Cheech can still go another day or two under the light with the lower pistols still a little blush.

Hello friends Still good with this plant, For the moment no problem with her, easy grow, she like be touched, and fertilizer. This week I've start pk 13-14. She have a really nice smeel and lot of pollen, I think bud will be not really big, because short vege, next time I'll put more plant. All continue to be good, I hope harvest for end of this month or start December. Next week I'll start to publish pic of trychromes. I'm in late in my diaries, my phone was broke and needing to got my pics in my old phone. Have a good week, grow and smoke. See you next week 😊😊

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Day 14 she’s loving life. I learned our ph was coming out at ph 8.5 or over so we got that handled and since she’s exploded. I’ve very excited to have her in the arsenal again.

Elle sent la mangue, le sucre, les tropiques 😍 Et ce champs de trichomes est superbe...

Zamnesia, obrigado por fornecer a genéticas para testes. Entrega segura, dentro do prazo e muito discreta, como você pode ver nas fotos. Experiência para possível plantio em massa, dependendo dos resolvidos. 1 BISCOTTI (ZAMNESIA SEEDS) FEMINIZED Já germinada 1 JEALOUSY (ZAMNESIA SEEDS) Já germinada 1 SUNSET SHERBET AUTOMATIC (ZAMNESIA SEEDS) FEMINIZED Será germinada no próximo mês, devido temperatura elevadas, temos dias chegar 40c⁰, não quero automática stressada, logo ao início. O diário será atualizado toda semana, espero que gostem (eu também).

Apricot looking healthy as , nice growth this week she’s loving the 15L pot I transplanted into this week and is a nutes pig , looking to double size again this week .

En la 6ta semana de vegetación hicimos una poda de bajos y desfoliación en general para potenciar el crecimiento de los brotes superiores, mejorando la iluminación y ventilación en el cultivo. También hicimos unos cuantos amarres (lst).

So heres the heavyweight Fast and vast. Id say im abit disappointed for the false advertisement on the heavyweight seeds range. It states that in good conditions the plants will be ready in 8-9 weeks with outstanding yileds. But fingers crossed, i cant talk big. Never know what these will produce! Day 35 from seed now and it’s already showing its pre flowers. I will update week 6 on tuesday! One Love

Will update with all the in

Iniziamo il risciacquo delle radici .. è carica di nutrienti ci vorrà un po' per smarrirsi tutti ora speriamo di aver azzeccato i tempi. Due settimane di acqua totale e poi si taglia!!

I've started decreasing my CO2 dosage as I have reduced my nitrogen load. I'm going to slowly keep removing any unnecessary vegetation. I have turned off my space heater at night to get colder temperatures. I started feeding extremely heroic doses of calmag (Rooted Leaf 0-0-0 CalMag plus) These buds are extreeeemely dense and covered in trichomes with a really gassy smell. Some pistils are starting to change colour to a deep amber.

Flowering Day 22 - They still growing very fast, the routine now is watering 3 times a week (2 with nutrients), on this week I add Azomite on soil, but they becoming thirst very fast. F Day 26 - Down leaves are becoming yellow very fast, I think Cinderella99 (the left one) Its more hungry. Water routine it is - 4 times a week, 3 with nutrientes (EC 1150), 1 only water, both pH 6.4 😁

I backed my light up a little and let my nutes sit for longer this time. She’s improving. Think I’ll up my ph on the next watering. Trichs are starting to come in I added a dish of water to up humidity. Don’t know if it’s helping. One of the pics has a blemish. It’s from me, not spotting from deficiency. She’s looking yellow and skinny. Stems got really dark

She’s starting to bush out started some LST great growth and healthy just add water

I’m excited to see what RQS have to offer with this little Gem! I’ve been waiting to try another of Royals Automatics, Fat Banana is a strain Im growing along side Gelato and Gorilla 😁 It’s been an eventful first few days.. (arnt they always!) she also poked her head up yesterday so Day 1 is 30/03/20. Let’s see how this week treats her, I plan to keep a close eye on the environmental conditions and to ensure she’s being fed some gentle nutrients, CalMag to begin with followed by some BioBizz Grow for the extra nitrogen needed at this delicate time 👌 See you all next week, I hope with more leaves to show 🌱✌️ Peace and love, stay safe