Defoliated her a bit. Now I have even canopy but I am concerned if I defoliated too late in the game because this is an autoflower. Only time will tell. Increased her feed frequency to twice a week. Seeing several cola sites. 01/25/23 : Did a second round of defoliation yesterday. She ended up being as bushy as before defoliation. Little concerned that the plant is vegetating but not growing buds. She is about to finish her 7th week. Almost acting like a photoperiod. 01/27/23: Nearing end of week 7. It feels like she is still vegging. I see some flower sites and some pistil hairs but no real bud yet. I am reading that some autos bloom really late. Trying hard to be patient with her and not just flip the lights to 12/12. Will give her couple more weeks to start blooming, if not then I will go to 12/12. The hardest part about extended vegging is light and air management. 😫. Oh the joys of growing your own!!

Gracias al equipo de Sweet Seeds, XpertNutrients y Marshydro sin ellos esto no seria posible. 💐🍁 Red Hot Cookies: Excelente genética resultado del cruce entre un clon seleccionado de una variedad “Super Strong” americana de la familia Cookies, la Tropicanna Cookies (Girl Scout Cookies x Tangie), y un clon seleccionado de una potente variedad también americana de excelente aroma a mandarinas, la Tangie. Para el cruce se han elegido exóticos parentales con fenotipo de flor roja. El color rojo en las flores se presenta en un 60% de la descendencia y suele aparecer en las últimas semanas del periodo de floración, comenzando desde las ramas y flores inferiores hacia las superiores. Variedad de marihuana “Super Strong”, muy productiva y resinosa, que alcanza niveles de THC de hasta el 25%. Excelente aroma y sabor, dulce, profundo y denso, con marcados tonos de mandarina y mango, y pinceladas de frutos del bosque. 🚀 Consigue aqui tus semillas: https://sweetseeds.com/es/the-red-family/3066-red-hot-cookies.html 💡FC6500: Eficiencia líder en el mercado: la lámpara de cultivo LED FC-E6500, que ostenta un estatus líder en el mercado, es una solución rentable con un PPE de 2,8 µmol/J y un rendimiento máximo de 2,5 g/vatio. Ofrece un PPF alto de 2026 umol/S y es adecuada para una cobertura de vegetación de 1,50 m x 1,50 m y una cobertura de flores de 1,20 m x 1,20 m. Iluminación versátil y uniforme. https://marshydro.eu/products/mars-hydro-fc-e-6500-730w-commercial-led-grow-light/?gad_source=1&gclid=Cj0KCQjw1qO0BhDwARIsANfnkv9IIrYSbmJqz8PqpJOIyWwJfp5bc3SGJgUV68USLQ4tjmXDYwoBuAsaAue3EALw_wcB 🚥 MarsHydro ADLITE UV/IR/RED: Para lograr un crecimiento óptimo de las plantas y maximizar los rendimientos es un arte simple que depende en gran medida de las condiciones ambientales adecuadas. Reconociendo las limitaciones de la iluminación natural y las soluciones de iluminación tradicionales para satisfacer estas necesidades únicas, lanzamos ADLITE. Estas luces especiales UV, IR y roja están diseñadas para llenar áreas del espectro, proporcionando las altas longitudes de onda que las plantas necesitan para un crecimiento y desarrollo óptimos. 🚀 Consigue aqui tu Adlite: https://marshydro.eu/collections/adlite-supplemental-lights/ 🏠 : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit 🌬️💨 Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 11: Última semana para uno de los ejemplares, la otra se va a quedar una semana mas todavía. Los tonos rojos en las flores son muy notables. Estoy muy contento con esta variedad.

Switched the LED to 'Flower' mode following 5 weeks of just 'Veg' light, she is showing signs of coming into flower now. She is short but bushy even after some defoliation. Looks like she has a few good bud sites developing. Bit of white tip on the edge of the leaves has appeared, not too concerned yet, will monitor and try and figure out what the problem could be...Possibly slight sign of 'nute burn' or N toxicity but i have been feeding her lite, i'm assuming the substrate is pos still 'Hot', rich with guano and mycorrhizal fungi. Took a cutting to clone from the Auto OG Kush earlier when i topped her just to see what she would produce. She rooted well and has filled the 1ltr pot. As expected going into flower the same time as the OG Auto mother. 17/9/18

*Week 3 forgot to add Humic Acid to PH Perfect nutrients. *increased nutrients by 1ml of each making it 2ml per gallon of micro,grow, and bloom. PPM seemed the same. *20-20-20 PPM from 300 to 350. *Lowered lights to 20 inches “Forgot to lower them” *increased light intensity by 5%-10% untill I reached 100%. *soil PH seems steady 6.5-6.8 *Cant really tell the difference between both nutrients both look identical. Can you? *watered about 32oz for each plant did not dry out previous week. So I did not water in betweeem feeding.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Muy buenas amig@s!! Preciosos los colores de esta cepa! Empieza a tener su olor tan unico, la que cultive en exterior olia igual y seguro que de sabor estara igual de bien o mejor!!

Massive growth on week 5 no any trouble

2020-02-18 (Day 81) - I spoke too soon. This girl is stretching WAYYYY more than any other girl in the tent, I have to raise 1 light daily it seems. All in all even with the asymmetry this is the most beautiful plant I've grown. As stated in my other journals my heater crapped out and I am not buying another one so she'll have to deal with the cold night time temps. Hopefully the stretching slows down and she can start putting all her energy towards bud development. This is the thirstiest of all the other plants in the tent drinking roughly 1 gal every other day. Stay tuned to see this girl develop!

wow!!!!! she's amazing :) her buds already look stiff, and she's still ripening :) Zamnesia Runtz auto is great for small spaces, I can also smell her amazing scent, I have no doubt that she'll have a great smoke :) the girl will only get 6.3ph water from now on, -washing :) I think she has about 2 weeks left until the finish :) I can't take all the credit for it, my Cat helps me :) watch the video :).

She looks ok 👍 Looks like beginning fase of amber, not yet, but still, very close! Lil bit.... Got a few days more

WC1 - 579g wet weight WC2 - 559g wet weight

Big buds nice citrus smell well THC coated.

everything changed on week 4 grow tent and cob led came to show how a good light is important. from now til last 2 weeks, only indoor growing goodbye CFL's, you worked well. + LST and ferts (2ml + 0.25) Defoliation started Removed branches from 1st node

All plants look happy, healthy and green. Added some cheap Wilkos Organic Plant Feed because why the hell not? Next 2 weeks will increase PK for peak flower. This plant is on the left of the timelapses.

Hello everyone 👋 She grew fast and with a beautiful green colour on the leaves! She has responded superbly to low stress training and topping & i defoliated her aswell and seems didn't even notice 😎 Wish you all a good day and happy growing 😁

Contando ya los días.. Prácticamente está todo hecho y sólo queda esperar. Ya sólo estoy regando con agua pura con el ph regulado. Estoy haciendo un lavado de raíces de dos semanas ya que busco el sabor lo más limpio posible. El clima del exterior está ayudando mucho y he podido mantener la humedad en los parámetros correctos. Espero cortarlas sobre el finde que viene Muy contento con lo que veo, la calidad promete 🤩

Blue Sunset Sherbert – Week 5 of Flowering The Blue Sunset Sherbert ladies are thriving! 🌿✨ Now at 4 weeks of flowering, they are developing beautifully. I noticed a slight nutrient deficiency in one of the plants, so I added a bit of Greenhouse Feeding Booster to balance things out. It seems to have worked well, and everything looks back on track. From now on, I’ll cut back on chemical boosters and switch to a more natural approach. Starting tomorrow, I'll be using my homemade calcium booster made from eggshells and vinegar to keep everything in balance. 🌱🔬 Exciting times ahead—stay tuned and don’t forget to follow for more updates! 😎 --- Blue Sunset Sherbert – Blüte Woche 5 Die Blue Sunset Sherbert-Ladies machen sich prächtig! 🌿✨ Jetzt, nach 4 Wochen Blüte, entwickeln sie sich wunderschön. Ich habe einen leichten Nährstoffmangel bei einer Pflanze bemerkt, also gab es ein wenig Greenhouse Feeding Booster, um das auszugleichen. Das scheint gut funktioniert zu haben, alles sieht wieder top aus. Ab jetzt werde ich die chemischen Booster zurückfahren und auf eine natürlichere Methode setzen. Ab morgen kommt mein selbstgemachter Calcium-Booster aus Eierschalen und Essig zum Einsatz, um das Ganze weiter zu stabilisieren. 🌱🔬 Spannende Zeiten stehen bevor—bleibt dran und vergesst nicht zu folgen für mehr Updates! 😎