Things are moving along in flower. Really pleased how these Rainbow Pie girls are really bulking up. They all seem to be praying up to the light and happy. the wedding cake pheno ended up being a real stretcher. She was the shortest plant before flipping into flower.

Zum Pressen waren beide Strains super. Angenehm zum Rauchen und ertragreich. Terps sind hauptsächlich Citrusnoten.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Will be doing a 12/12 as soon as my pineapple x Wilson and sweet zombie get to week 4. Nothing but rupees in this grow tent.

This plant is getting really big it looks lovely through out about to start feeding a little lighter do when the buds come in the smell is beautiful it’s smells gassy & stinky. Week 5 check out my other grows (Jungle Verde, The New) & follow my IG @therealterpio

Thought I would of got more but the terps from her are unreal can’t wait to smoke

Always rock hard buds💥 Sticky glue👀 The smell is divine & refreshing : imagine a walk in a pine Forest in Summer with hint of lemon🤤🍋🌲 Moon safari effect

Venerdì 12/01/2024 Inizio controllo ec 778 ph 6.6 Non Aggiungo acqua perché c'è ne in abbondanza. Aggiungo alla soluzione dose NPK per 25lt 45 Grow 30 micro 15 bloom Ottenendo ec 1150 ph 6.3 Integro: 30 sensizym 30 calmag 30 rhino skin 30 nirvana 30 ml tarantula 30 ml voodo juice 30 bud candy Ottenendo ec 1335 ph 6.2

Love this lemon strain , starting to get some nice colour and phatting up real nice . 2 weeks to go. Legendary genetics

Week 8 day 3, preparing for flush for week 9 and chop week 10

Welcome to the start of week four. We join her royal majesty as she begins what I suspect will be a big week. She has continued to impress every day and I am increasingly excited to see what she can do. I'm beginning to dream of my next grow [G3] a tent full of princesses. I still do not know what effect the attempted FIM has had but I suspect by the end of the week it will be obvious. Day 23: Secondary shoots have overtaken primary. I'm curious as to how the FIM pans out. Day 24: LST on lower nodes. Day 25: Citronella continues to show mortals of lesser breeding what royalty can do. Day 25.5: Scalpel! Wire! LST! HST! Defoliate! Defoliate! Defoliate! Her majesty has been adjusted (per royal decree). Day 26: Her majesty is thirsty... I'm having to feed her twice a day. I need to move her into a bigger pot. Day 27: Accidentally snapped off a side node attempting HST. Have dipped in rooting hormone and potted. Perhaps this will be the first Princess. Day 28: Photo before lights on hence wilting. Weekly summary: Because I took off four really big leaves Citronella's progress doesn't seem as impressive this week. However let me assure you it has been transformative. Her stem is thick, her colour is perfect, she responds willfully to my attentions (training). She is a mother plant so everything I do is geared towards producing more and stronger side stems.

--- Day 43 --- 15/06/2021 Starting to water 3L today, she's looking really good. Shes finally starting to stretch out a bit, up till now her canopy was quite crammed and close. Some of her branches are starting to grow up and the whole thing is stretching out a bit, which is nice. She's now 51cm/20" tall. I've watered her again with 3l of 23C, 1000PPM, 6.0PH water with the above nutrients --- Day 44 --- 16/06/2021 I'm really happy with the way her canopy shape is looking, it's all pretty even and level, considering the accident I had, I think she's doing just fine. I've watered her again with 3l of 23C, 1000PPM, 6.0PH water with the above nutrients --- Day 45 --- 17/06/2021 She's now just over 23" tall and she's filled the tent. The light is as high as it can go, and she's touching the walls of the tent. She can't get any bigger, else we have issues. I'm going to have to get a bigger tent... I've watered her again with 3l of 23C, 1000PPM, 6.0PH water with the above nutrients --- Day 46 --- 18/06/2021 Shes now over 24" tall and she's filling the tent quickly. Also, I noticed today the first signs of her going into preflower, you can see really small white pistils showing 😀 I've watered her again with 3l of 23C, 1000PPM, 6.0PH water with the above nutrients --- Day 47 --- 19/06/2021 Shes growing really well, there are so many bud sites growing on her now, it won't be long before I'm watering 4L. I've watered her again with 3l of 23C, 1000PPM, 6.0PH water with the above nutrients --- Day 48 --- 20/06/2021 Shes starting to get way too big for the tent, I'm going to need a larger one. The humidity is also worrying, it's constantly at 98% even with the dehumidifier and ac on. I've watered her again with 3l of 23C, 1000PPM, 6.0PH water with the above nutrients --- Day 49 --- 21/06/2021 So, I got a new tent. I went from a 60x60x160cm one to a 90x90x200cm one. Ive also upgraded to the AC Inifity T4 extractor Fan system to help with the ventilation and humidity issues, it's really cool. I'm going to start watering 4L tomorrow. She's looking really good. I've watered her again with 3l of 23C, 1000PPM, 6.0PH water with the above nutrients

So I changed lights this week to 600 w hid Hood is air cooled so we’ll see how this finishes! Everything is going great, buds getting bigger every day.

Cherry is quickly making me forget all about the tragic death of her sister. She is really bulking up and starting to really put on the frost even some fan leaves are starting to become frosty. She may have started slow but MAN is she making up for it now.

Sulla più piccola sono arrivate le mosche bianche per sfortuna, spero che non invadono le altre, non da nemmeno segni di sviluppo la Cherry, peccato.. Le altre in grande forma, soprattutto il mostro di skittles! Ho annaffiature solo 2 volte con noci questa settimana il resto solo acqua, non voglio stressante troppo, ormai è ora della fioritura per loro ❤️ Spero che non continuino a nutrirsi delle mie piccole..per ora gestibile, stiamo a vedere. Le infiorescenze iniziano a ingrandirsi, il profumo anche, meravigliose.

Plantas sanas creciendo a full. Satisfecho hasta el momento con el resultado.