17.05. I don't have anything special to say, of course the weather is terrible... it's 6-7 at night, 15-16 during the day, so they're inside... the temperature inside is 20-21 during the day, at night from 15 to 17 when it's this cold... the plants were exactly 3 weeks old yesterday, today they entered the fourth week and most likely they will wait inside for the fifth week as well because the weather is terrible and there is no point in transplanting yet. Stay High!

Buenas a todo compañer@s,otra semana más de cultivo. Amnesia haze Hoy día 3 Finalizamos la semana 5 de floración. Cambiamos a la semana 6 Aumento de la EC hasta los máximos valores que acepte la variedad,proabado este fenotipo de amnesia haze en varios cultivos con una EC2600 en sustratos. Una semana bastante jodida de agua,muchas lluvias,con lo que conlleva alta humedad en el exterior y con ello más alta en el cultivo. Pero seguimos luchando! Vídeo subido en la madrugada . Dia 5 Todo bien ,exceptuando una de las niñas que parece tener alguna deficiencia,magnesio pensé en primera estancia,pero otros compañeros me dijeron que podría ser que el magnesio bloqueó el potasio en unos riegos atrás. Le reduciré el magnesio,y le seguiré dando la misma dosis de potasio que le doy a todas las demás.. El riego es por goteo y no queda otra, a esa pequeña le añado lo que pida de más con una pequeña pistola de agua por debajo. Va afinando el cultivo con respecto a los olores. Día 6 Al fin,puedo volver a decir ,tengo todo bajo control!! Al menos por ahora.. Correji,el problema de potasio de la planta con la deoficiencia que no sé cómo llegó a ese punto,tan pronunciado.. hoy quedó la cama regada con una buena cantidad de abono EC2200 En una cantidad de tanque de agua de 44 litros Un ph6,2 controlado como en todo el ciclo de floración.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welcome to 📅 Day 1 of veg 3/15/2021 as you can see the seedling has grown a lot she had a 1 inch root sticking out the bottom already so I know the root starter was working. If you look close you can see she has her first true leaves and that's when I say day 1 of veg has started. I will do the video tomorrow germination under the mars hydro TS 1000 was a dream the dimming gives it a hand up over my Aglex light. I am growing the plants side by side this one is under the TS 1000 and the other will be under the Aglex 1200 to see how they compare. I have high hopes for the TS 1000 🌱🌱🌱 Update 📅 Day 3 of veg 3/17/2021: she is coming along very nicely her first true leaves are getting very large. I have the TS 1000 putting out 13253 lux. I'm top feeding her for about the first 2 weeks so the roots get down in the soil far enough for the auto-pot to do its job Update 📅 Day 6 of veg 3/20/2021 she is growing fast and strong under the TS 1000 the second set of leaves has started and progress is great I adjusted the lux down a bit

10/11/24 she had gotten so overly dry that she was impossible to water. i had to scrape and break of the dry spots all around the soil and root ball so that water could penetrate. she was root bound from a solo cup and then as well from 1 gallon to 3 gallon. once the rootbound spots got overly dry, they became super hydrophobic and i didnt realize how bad it was. that may be what's been affecting the over all health of the plant. finally able to get her to hold weight in water so im hopefull 10/15 pistils are getting thrown out everywhere. a bit of a stretch so far but not too much. maybe a couple inches or

Hey good morning Growers hope all is well with everyone and their family and plants Well there’s a lot going on over here at the 48219 Krud Buddy’s Grow House I found out when I transplanted my Wedding cake and Sherbertlato I put them into super soil I feed them twice lightly thank god cause I don’t have any burns or anything n their growing good to me everything is growing good to me way better than the auto flowers I started with but I did some cloning of my own and I’m loving how their taking on as well So here’s what’s going on strain by strain Gorilla Cookies & Peanutbutter Breath : as of Today April 25th they are on day 60 since I got them and have been sitting in the dark since last night for their 24hrs of darkness before flip to flower starting tomorrow morning thinking a good timing would b from 6/6 or 7/7 also I have them under my 600w budget led hanging 12’ above I can’t wait to see how they out and I have them under an scrog net I made Wedding Cake & Peanutbutter Breath: on May 5th it’ll be 60 days since I’ve had them I pretty much told everything bout them at the beginning Grape Pie & Girl Scout Cookies: it’s Been about 3 weeks And I must say they stretched fast their already 15’ tall the grape Pie is spreading faster any of the other clones I got I’m loving it I recently got Cherry Pie & Guava Cake x Cookie it’s bout week one going on two weeks and I must say when I got them I was kinda skeptical about them because they were so weak looking and the cuts on them were terrible but they look like they might turn out into something amazing idk but the pictures show it all as well as the clones I cut myself in clear plastic cups ands their on their second week of life I noticed roots on the 10th and put into cups on the 11th if I’m not mistaken I’m not around my chart so I’m not for sure but everything is going swell over here to me

Nothing major happening I just started the mainlining process I'll keep you guys posted

Week 8 (17-3 to 23-3) 17-3 Temps: 17.4 to 21.9 degrees Humidity: 60% to 72% 18-3 Temps: 17.5 to 22.2 degrees Humidity: 54% to 65% 19-3 Temps: 17.9 to 22.4 degrees Humidity: 52% to 61% Watering: Both 500 ml. EC: 0.4 Watering: Both 1000 ml. EC: 1.4 Defoliated both plants and attached some shoots to the rack. She got really big fan leaves! I also supercropped some branches. 20-3 Temps: 18.2 to 22.8 degrees Humidity: 59% to 69% 21-3 Temps: 18.5 to 23.4 degrees Humidity: 57% to 67% Watering: Both 1000 ml. 22-3 Temps: 19.4 to 24.1 degrees Humidity: 55% to 67% Watering: Both 1000 ml. Set the strength of the light to 50% 23-3 Temps: 19.8 to 24.8 degrees Humidity: 58% to 67% Watering: Both 1000 ml. As you can see, instead of removing the leaves, i tucked down all the leaves to open up the canopy. I will see if i like this method as its the first time i try this. I also attached some more shoots to the rack, its looking nicely spread out now :) The #2 makes my fingers very sticky and smells really strong! I love it!

Hello. This is the end of week 9 and the beginning of week 10 of flowering. Everything is going good here. The plants have had 2 good watering's with just water, to use up that extra nutrient that is in the soil. It's not enough food for the plants left in the soil, so they start pulling some of their sap from the fanleaves and the leaves go yellow. The buds are sure bulking up now. They are twice as big as they were 2 weeks ago. One more week of flowering. then a couple of days of dark and then harvest. I sent an email to [email protected] asking if it's OK for me to give the 50% off code to someone else. They said... Yes.. it's OK to give it to someone else on grow diaries. They would love it if diaries were made for them. But... Yes. So if your interested... send me a privet message... so I can find out how many people are interested. That's a 50% off your first order, code... and it has to be used by May 1st 2026. That's... www.ekhta.es/ to check out their strains. OK. Be Cool. Chuck.

Another awesome week as these buds are getting dense and Frosty

02/11/2021 Doing water cure

Hallo zusammen 🤙. Sie wächst sehr schön und macht keine Probleme.

Critical x Animal Cookies is growing great under the Hortibloom Solux 350. She just had a solution change today. I turned the light up to 100 percent as well. Nothing else much is going on at the moment with her. Thank you Hortibloom, and Ripper Seeds. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Day 35. Topped her yesterday above the 3rd node and spread the branches out with ties so the lower growth gets more light. Photos are from today, one day after the cut. The big fan leaves had started to go pale and mottled between the veins, which I took for a magnesium deficiency, so I gave her Epsom salt. The leaves in the photos are still showing it, I’m watching the new growth to see if it comes in green. Tent has been sitting around 24 °C and 64% RH for the last week and a half, air VPD around 1.1 kPa, soil moisture 49%. She’s in a 10 L fabric pot with the organic mix, watered through wicks. A new 14 day timelapse is running.

📆 Week 14, 5-11 August 2024 5-11 Aug - Observed and let the plant grow. 📑 Pablo continues to grow! She has not slowed down in water consumption so she may need more time than expected. I’ll have to observe for another week before a decision can be made when to harvest. Being 90% sativa this is not unusual. Pistil hairs are about 75% brown (with new ones developing). Buds are bulking out, continuing to grow and swell, and trichomes are building. The smell keeps intensifying with that of a lemony sour pine. 🍶 6, 10 Aug nutrient solution changed 🍽️ 6, 10 Aug feeding schedule updated 💧 Using reverse osmosis water with EC/TDS at 0 🐉 Nutrient Solution EC 2.0 at 75 degree F 🔆 Light power at 65%, DLI 40 canopy coverage at 12hrs 😤 Using PYPABL, Air Pump, 400GPH That is it for this week. Thanks for the look, read and stopping by.

Beautiful blooming Golf ball size..🏑⚾️ Nutrients Plagron on bloom is very very good. 👌 Alga Bloom 4ml/l 🍡🥜 Royal Sugar 1ml/l 🍭🍯 Green Sensation 1ml/l 🥦