13 Agosto 2024. selección de semillas y colocación en vaso con agua ph 6.0 con agua Oxigenada. 14 de Agosto se ponen en agua las semillas por 24 horas para hidratación. 15 de Agosto, con las semillas con muestra de radicula procedemos a pasarlas a papel absorbente. 16 de Agosto se muestra la radicula avanzada. 17 de Agosto en la mañana se puede apreciar el crecimiento de la radicula. 17 de agosto en la noche trasplantamos a "Peat Pallet" y una a maceta de cartón ya que la radicula creció bastante. 18 de agosto ya en los "Peat pallet" se pueden mostrar qué las semilla se cayo por completo e iran abriendo en el transcurso de las horas, las tenemos en un domo germinador dentro del Indoor. 🌡️ 27.1 ⁰C 💨 75% Humedad 🌫️ 0.9 VPD kPa.

This grow was pretty simple ! They went a total of 103 days from seed! Very trichomy dense buds with the smell of Berries vanilla and skunk ! This is a must try for you all Fastbuds has the greats!!!

Space is limited and I'm struggling with the time to keep up with these plants since things are back to normal on my parts of town. Just gonna keep about eye on em at keep defoliating. Plants flipped after 1 month veg

Was just using water with the last 2 plants this week. They both plumped up a bit and got more color going on, which is exactly what I was waiting for. Overall, it was a nice grow with only a couple hiccups.

Nach 18 langen Wochen ist die letzte Pflanze endlich fertig! Alle Sorten waren richtig lecker Am besten waren euforia und Passionsfrucht. Auf zum nächsten grow 💪💪

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

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Week 7 update on these beautiful ladies. They're progressing perfect and swelling on a daily basis. Wild to see the colas change from day to day. Some are a lot frostier then others but they all have that amazing fruit, sweet, and pungent terp. All in all Happy

we are at the beginning of the 5th week. the LST training is working quite well so far. it has become a bit caotic. it is possible that i have overfertilised her a bit, she has got two yellow spots. but nothing wild yet. she still gets canna rhizotonic, biobizz grow and biobizz bloom. i don't do any exact fertilisation, more like a guess. she has the first flowering starts. let's see how she develops further. :)

ladies have started to take off much more this week. I added heavy 16 finish to schedule of nutes. may be adding fire and roots also over next weeks. no huge issues this week, felt pretty dialed in for most part. using grow room glasses ive found i can get decent video of the girls under lights vs having to take them oit find ok lighting and taking a pic. noticed smell coming off the plants this week, its weak right now, but definitely starting to stink! going to top at the 3rd node once they reach 8 inches tall. after day 28, I transferred into 3 gallon fabric pots, which will be their final homes til harvest! (bottom leafs on plant #1 got some spotting, one side was windburn from earlier in grow, the other side I got a few drops of nutrient mixed water on them, and even after wiping away it left spots on leaves, its all good they are going to be removed after topping)

9/14 Week 9 and they are doing fine Little vid Hero Left rear Bea rt rear They are almost identical now Bea a bit larger Nuets not changing for now 9/16 Doing well but some white tips are saying we gotta reduce the salts so will be dropping CT nuets 10% and upping PK to 2.5 Extra space is really doing them well Defoliation again tonight the war on stemmed leaves has begun 9/17 Held a meeting of the minds and decided the best course of action is to flip the light schedule so I can feed them more appropriately, twice a day. Leaving nuets as they are as this will drop pot EC. Setting the alarm for the AM before lights out at 7:30 and changing the sched to 6AM-6PM summer is done no need for current schedule. Lights be out from 7:30AM to 6AM long I know but should not bother them. Made a bouquet of all the sucker branches, seems to be a lot on FF11 9/19 Lights on girls feed buds look fine Video enjoy

Ça prend forme, et ca prend des couleurs, rien à signaler à pars que je m'attendais à pire vu les problème en croissance, cette semaine on a progressivement baisser les engrais et la température. Mon chat tiens a vous présenter ses excuses pour les 2 poils sur la calypso sunrocks, c'est entièrement de ça faute 🙈🙀😻🤣

Nothing to say about this week. They are growing very fast. A strech is noticeable and first signs of buds are visible. Hope they develope fast. The next week i will add BigBud

Ooooooweeeeeee they’re all doing so good even the extras that I threw in flower with out cloning are doing great .. in just 2 more weeks I’ll throw the donor plants into flower

Bene bene scusate il ritardo 😌 di un giorno come nell' altro diario.. purtroppo ho problemi in famiglia con mia nonna che nn sta molto bene e non ci sto molto con la testa e nn riesco a stare dietro come meriterebbero l attenzione massima tutti i progetti. Anche nelle descrizioni , considerazioni, opinioni , mi piacerebbe avere più tempo x farlo x descrivere davvero bene come le ragazze stanno esplodendo di vita ! Apparte questo velocemnte posso dire che la ragazza che sta crescendo con un soil dinamico sinergico creato con micorizia e funghi benefici, melassa e comunque tutto completamento organico, della linea di Bio Tabs , sta crescendo davvero bene , un verde intenso sintomo di prosperità, ho solo aggiunto 3 ml di pk bio , ma 😅 potevo anche evitarlo dato che stava già benone!! E si vede nelle foto non c'è nulla che posso dire di negativo su di lei per ora ! Mentre la secondo ragazza , io nn avendo mai usato il nutrimento della linea RQS, probabilmente sono stato un po' leggero con i nutrienti e si vede che è andata in carenza di calcio e magnesio . Ora ho provveduto ad aggiungere al nutrimento solito che gli do, quindi vediamo come va!! 🙏 Ringrazio come al solito la luce fantastica che fa prosperare le mie piante come fosse un ☀️ in miniatura!!! Mars hydro la migliore luce che posso consigliare a tutti se avete un grow box piccolo come il mio o cmq anche più grande da un metro x metro e altro 180 cm o 200 cm sarebbe l ideale.. ma 😄 purtroppo ora nn posso permetermela quindi va bene così ! Ringrazio RQS dei semi spettacolari che consiglio a tutti! Buon 420 a tutti! 🐱😽💪👊🧙‍♂️🌱🌿🌲💨

had to change nutrients bc i didnt had enough so i had to buy a good bloom fertilizer in panic i chose hesi bc its relieable left side acid dough right side black domina

Update of week 2

Future is loving the tent to herself, room enough to spread out and phatt up . She’s pungent as , sticky, and colas are like 3 ft long . This strain promises high THC and by looks of it I’m gonna get a bumper yield in around 2 weeks . Can’t wait for my time space continuum to be fucked up 😂😂😂😂😂