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1. The workbench and all gears were cleaned with water. Then sprayed and wiped with 99% isopropyl alcohol to sterile.
2. A 400ml of 0.5% H2O2 solution was made by diluting 4ml of 50% food grade H2O2 with 396ml of Reverse Osmosis (RO) water.
3. Seed was submerged into 15ml of 0.5% H2O2 solution in a corresponding glass jar for 12 hours to soften its shell and prevent it from bacterial attack.
4. A Root Riot cube was also submerged into 0.5% H2O2, but in separate container. It was submerged for 12 hours to sterile.
===== 12 HOURS LATER (12/13/23 11:16 PM) =====
5. Germinating temperature is recorded at 21.7c. RH is irrelevant since a humidity dome will be used.
6. Root Riot cube is picked out off the H2O2 bath and drained off excess liquid by shaking (!!!NEVER SQUEEZE THE CUBE!!!).
7. Seed is sowed ~0.5cm into the opening hole of Root Riot cube.
8. The cube is placed into the tray and labeled along with two other seeds.
9. Humidity dome is put on with all ventilation holes closed.
===== 72 HOURS LATER (12/16/23 10:56 AM) =====
10. Seed reoriented itself. Good sign. Expect a sprout within 24 hours from now.
===== 86 HOURS LATER (12/17/23 01:20 AM) =====
11. Young sprout is supplied with 120 µmol/m2/s of light from two T5 LEDs under 24/0 schedule.
===== 95 HOURS LATER (12/17/23 10:35 AM) =====
12. Humidity dome is opened 10%.
===== (12/17/23 07:06 PM) =====
1. Seedling is moved onto DWC system running 0.01% H2O2-treated seedling strength nutrient solution @ 340 PPM, PH 5.9.
2. Seedling is supplied with 140 µmol/m2/s of light from the main grow light under 24/0 schedule (DLI: 12.1 mol).
3. Climate is recorded at 22.2 degrees Celsius, 75% RH, 1555 PPM Co2.
===== 28 HOURS LATER (12/18/23 10:51 PM) =====
4. Nutrient parameter is recorded at 370 PPM, pH 5.8.
5. Climate is recorded at 23.1 degrees Celsius, 66% RH, 1520 PPM CO2.
===== 25 HOURS LATER (12/19/23 11:39 PM) =====
6. Nutrient parameter is recorded at 370 PPM, pH 5.8.
7. Climate is recorded at 23 degrees Celsius, 68% RH, 1425 PPM CO2.
8. Leaf temp: 21 degrees Celsius, VPD 0.58 kPa.
9. Light intensity is increased to 190 µmol/m2/s (DLI: 16.42 mol). Light height 19.5 inches.
10. Co2 intensity is increased to 550 PPM.
===== 21 HOURS LATER (12/20/23 09:00 PM) =====
11. Nutrient parameter is recorded at 360 PPM, pH 6.1.
12. Climate is recorded at 24 degrees Celsius, 63% RH, 870 PPM CO2.
13. Leaf temp: 22 degrees Celsius, VPD 0.82 kPa.
===== 39 HOURS LATER (12/22/23 12:45 PM) =====
14. Nutrient parameter is recorded at 370 PPM, pH 5.6.
15. Climate is recorded at 25 degrees Celsius, 73% RH, 1135 PPM CO2.
16. Leaf temp: 22.5 degrees Celsius, VPD 0.54 kPa.
17. Co2 intensity is increased to 600 PPM.
18. Leaf symptom observed. Probably because humidity too low and the root does not reach water line yet / humidifier ran out of water for around 4 hours during 05:00 - 09:00 AM.
===== 11 HOURS LATER (12/22/23 11:20 PM) =====
19. Leaf temp: 22 degrees Celsius, VPD 0.48 kPa.
20. Climate is recorded at 25 degrees Celsius, 70% RH, 1055 PPM CO2.
21. Nutrient parameter is recorded at 370 PPM, pH 6.0.
===== (12/24/23 11:50 PM) =====
1. Light intensity is increased to 235 µmol/m2/s (DLI: 20.3 mol). Light height 16 inches.
2. Nutrient parameter is recorded at 370 PPM, pH 6.0.
3. Climate is recorded at 26 degrees Celsius, 73% RH, 1170 PPM CO2.
4. Leaf temp: 21 degrees Celsius, VPD 0.62 kPa.
5. The new set of serrated leaves (3 fingers) looked normal as it should.
===== (12/26/23 11:28 AM) =====
6. CO2 intensity was increased to 650 PPM.
7. Nutrient parameter is recorded at 370 PPM, pH 6.1.
8. Climate is recorded at 26.5 degrees Celsius, 67% RH, 858 PPM CO2.
9. Leaf temp: 23 degrees Celsius, VPD 0.51 kPa.
===== (12/28/23 12:45 PM) =====
10. CO2 intensity was increased to 700 PPM.
11. Nutrient parameter is recorded at 370 PPM, pH 6.1.
12. Climate is recorded at 26.5 degrees Celsius, 57% RH, 825 PPM CO2.
13. Leaf temp: 22.5 degrees Celsius, VPD 0.75 kPa.
===== Entering WEEK 1 of LATE VEGETATIVE stage (01/12/24 5:39 PM) =====
1. CO2 intensity was raised to 1300 PPM.
2. Grow system was flushed and filled with 0.01% H2O2-treated late vegetative strength nutrient solution.
===== 01/13/24 02:36 PM =====
1. Nutrient parameter is recorded at 840 PPM, pH 5.6.
===== 01/15/24 06:25 PM =====
1. Nutrient parameter is recorded at 840 PPM, pH 5.7.
===== 01/17/24 12:27 PM =====
1. More pruning and LST
2. Nutrient parameter is recorded at 900 PPM, pH 6.0
===== 01/18/24 09:53 PM =====
1. More pruning and LST.
2. Nutrient parameter is recorded at 950 PPM, pH 5.8
===== 01/19/24 09:05 PM =====
1. More pruning and LST.
2. Nutrient parameter is recorded at 980 PPM, pH 6
===== ENTERING WEEK 2 OF LATE VEGETATIVE STAGE 01/20/24 =====
1. More pruning and LST.
2. Nutrient parameter is recorded at 980 PPM / pH 6.0
===== 01/21/24 08:51 PM =====
1. ScrOG net is placed.
2. Nutrient parameter is recorded at 1010 PPM / pH 6.1
===== 01/22/24 06:49 PM =====
1. 1 gal partial reservoir changed.
2. Nutrient parameter is recorded at 1020 PPM / pH 5.8
===== 01/23/24 09:57 PM =====
1. Nutrient parameter is recorded at 1010 PPM / pH 5.9
===== 01/25/24 12:29 PM =====
1. More pruning.
2. Nutrient parameter is recorded at 1030 PPM / pH 5.9
3. 1 gal partial reservoir changed.
===== 01/27/24 07:32 PM =====
1. More pruning and scrogging.
2. Nutrient parameter is recorded at 1060 PPM / pH 6
===== ENTERING WEEK 3 OF LATE VEGETATIVE STAGE 01/28/24 09:07 PM =====
1. Light intensity was adjusted to 465 umol/s/m2 (DLI: 40.18 mol).
2. Nutrient parameter is recorded at 1050 PPM / pH 5.9
3. More pruning and scrogging.
===== 01/29/24 05:39 PM =====
1. More pruning and scrogging.
2. Nutrient parameter is recorded at 1030 PPM / pH 5.9
===== 01/30/24 02:51 PM =====
1. Full reservoir changed and topped off with 0.01% H2O2-treated late vegetative strength nutrient solution.
===== 01/31/24 06:47 PM =====
1. Nutrient parameter is recorded at 1000 PPM / pH 5.8
===== 02/01/24 11:16 AM =====
1. Nutrient parameter is recorded at 990 PPM / pH 6.2
2. Light schedule is toned down to 23.2/0.8 to limit the DLI to 38.8 mol.
===== 02/02/24 10:46 PM =====
1. Nutrient parameter is recorded at 1030 PPM / pH 6.2
===== 02/04/24 12:11 AM =====
1. Nutrient parameter is recorded at 1090 PPM / pH 6