m0useanswered grow question 8 months ago Simple answer "no"
This is why autos are recommended to new growers as they take the complication of photoperiods "light cycles" out of it. but they can add in a whole new set of complications from autos just being autos.
Autos don't require darkness to enter flowering stage likes their photoperiod counterparts. Flowering hormaons only build up in darkness in the Photoperios plants as the light disregulates one part of the hormones being formed. Autos do this normally regardless of light, how? I don't fully know but they do.
A note on darkness, Darkness for plants is not absolute zero photons. The moon casts a bit of light. One simple test is this. this was suggested by dr.Brusebugbee a cannabis researcher, if you hold a 10pt text book from your face at arms length and you can clearly read it, there is to much light. Our eyes are great at seeing small amounts of light and making it work, aka moon light, but shit with bright lights and registering the intensity. that dr has a whole set of youtube clips on lighting in cannabis, debunking green light does not trigger photosynthesis and alike. He has a number in PPF that has to be below for the cannabis plants he studied to be considered night. I can't recall it. but the book test is a good no tech test.
With that said, the plants that grow outside get uninterpreted darkness normally in the wild unless their is human involvement with street lamps and such, I have been in the country side where there is no town or city for miles aorund and it gets pitch back dark moons helps a bit but again we are great at seeing in low light conditions but ulta low light not do much, need cat eyes to do that.
there is a good point about the Calvin Cycle, I myself can say I don't know much about it and will look into it, it seems to be describing how the plants use co2 and turn it into sugar. I am a firm believer in giving plants darkness so they can rest and do their own thing. I have seen many plants on 24/0 not looking so hot, and then when the grower switches to 18.6 or 20/4 they start to bounce back and look healthier. nothing else was changed just the light schedule.
Take from this what you will. outside of your question, I find it interesting a greenhouse would grow 4000 autos at a time when photoperiods are much better yielders and can tolerate more stress without fucking up. can also clone them for stability in a mass crop and quality. Maybe that greenhouse does breeding or photo hunting or can't provide the darkness levels required for flowering. Very interesting non the less. I'd be curious to know why autos vs photos though.
Good Luck!