Germination issues

Hello You have to be careful with the temperature and don't leave it below 22 Celsius. Also, if you have poorly stored seeds, they may never open.

I doubt its the 24hrs as Iv done it a number of times with no ill effect.You could try scarifying them,not sure on spelling.The shell can get hard so need some help.Old hippies used to crack them withtheir teeth.Scarp them with a fine mettle file,nail files work well.Make sure you leave your wtaer to stand for 24hrs from start bto finish.The seeds have microbes and chlorine kills them and that includes the microbes in the soil.Seaweed helps with gemination.Iv not grown without it for over 10yrs.

Ya might have drowned them. Try 14 hours next time for the soak. Also you said tissue. Hope you meant paper towel. Use spring water Sermon: Double paper towel, fold like a burrito, place towel on a ceramic plate, open folded flaps, pour glass of seeds into towel, fold back up, pour spring water on paper towel, drain excess and put on a heat mat in a warm dark room. Check moisture morning/evening. 80% 1-2 days SORRY FOR TAKING YOU TO SCHOOL!

I find soaking for 24hrs is to long and sometimes it hurts the seed. also if you are soaking them in really wet tissue it could be to much water. Like others I try and simplify the process. soak 12hrs then into soil. It does me well. also done direct to soil but last run I had a lot of issues with my soil. I also like to keep my seeds in 21c or more for germination. after they are established they can drop down to 18c if need be. Give it some more time. if nothing happens document it and present it to RQS for review.

patience grasshopper. Some seeds take a while - usually older seeds. when you get to 10-14 days (maybe less with this paper towel method) then you can worry about it being inviable. I'd definitely suggest simplifying your process. Especially if you have a hankering to fiddle or look at it constantly. You need to learn to plant it and walk away so it can do its own thing. you have no influence over this process beyond the simple things. don't change this midstream.. keep doing as you were for this cycle. Temprature helps. A 75-80F substrate definitely improves consistency of spawn times. Again, it will not put lipstick on a pig, but for anything healthy enough to sprout, it'll shorten the range of time it takes for several seeds to sprout. A heat mat with a thermostat is useful, but don't go cheap and skip the soil probe thermostat option. A humidity dome can help you avoid watering during germination process. Once it's sprouted, no worries, but if it is still below ground having to water before it sprouts can cause some shifting around that isn't always ideal. water slowly and arouand edges to allow it to soak in as opposed to causeing anything to float if you do have to. Remove humidity dome when they sprout. You'll want this for clones, too (both dome and heat mat), so it'll get used plenty. Soaking for up to 24 hours is fine. Might help with older seeds. Simply fill a small seedling pot and tamp it down. Press finger down in middle about 1/2" -- this compresses the substrate a bit where your seed will rest. Gently push the substrate over from sides and tamp down lightly. that's it... none of this 5-step wet tissues with bleach and other chemicals in it. no exposing tap root to light. no touching a very delicate and recently germinated seed to plant it. All of those behaviours add needless risk of a seedling failing. i don't worry about crown orientation too much. i just make sure that it is horizontal as opposed to upside down. vertical tends to leave more seed shells on when they sprout, and that's a delicate procedures thats best avoided. how many seed shells you see stuck on a sprout will help you adjust how deeply you plant the seed. Too deep it takes forever, too shallow it has a seed shell or worse the taproot dries out.

I agree it's probably temp related, if you're absolutely sure the seeds aren't cracked or anything. I like to just do an overnight soak to soften the shell and then it goes straight into my rooting pucks(I use Rapid Rooters). Generally they're popped up 24-36 hours later but cool temps can stall or even stop the process. Sometimes seeds can take awhile though, especially if they're old or something. 1-2 week germination times aren't unheard of.

Plant straight to soil. There is too many variables, that could lead to mistakes while you manipulate seed a lot ... keep it simple.

What temperature is it sitting at? I find if I put on top of my fridge the heat speeds it up a bit. How old are the seeds?