Ca deficiency are yellow or brown spots. Google image search a marijuana leaf symptom chart -- high res so you can zoom in and look at each individual example.
Your RH is probably a bit high. While VPD is the primary target of Temp and RH, you don'tn want to go over 65% for prolonged periods of time. A spike is manageable, but consistently doing so will increase risk of mold.
Adjust temp and rh based on VPD and current life stage - lots of references for suggestions on this. FYI, they usually reference leaf surface temperature, not atmospheric temperature -- any professional source will be referencing leaf temp. User-submitted infor could be either. The offset is 3-5F cololer than atmosphere. While i wouldn't put any weight into exact recommendations, the gist is you want a bit lower vpd in vege than in flower. Should vege be .8 kPa? 1.0kPa? This is species specific and i don't see any super dependable data about this specific plant and VPD, but it may exist if you look harder than me.
It may not affect all plants equally, but what it definitely does is increase consistency of a wide range of plants. Less volatilty is good.
A little chlorosis in new growth is fine as long as it quickly fills in, which seems to be the case here. Plants looks healthy. Looks like these pictures are taken with normal lighting. Be aware under grow lights everything looks paler than reality.
Leaves do more than capture light. Get the idea out of your head that you need to remove a leaf because it doesn't get much light. If the plant deems it a 'sink' instead of value-added, it will shed that leaf and you can pull it off once it sucks all the nutrients out of it. Also, if you don't over-crowd the canopy, there's no reason to remove leaves for light penetration reasons. Light capture is much more efficient closer to the light than further away, so maximingn light at lower levels results in less energy from photosynthesis produced, or worse... creates holes in the canopy where light completely misses the top of leaves --- tops of leaves is the key for light absorption, not other plant material, like stems or flower with 1/100th the chorophyl.
With proper canopy management and not over-crowding, you should never have to do any major defoliation. Pruning off growth that you have no use for and selectively pruning off a leaf causing a real problem may happen. The latter of which can be mostly avoided. The previous depends on how large you grow a plant. The larger you grow, th emore wasted growth you probably have to prune off givin dynamics of indoor grwoing -- A limited depth of good buds resulting compared to outdoors.
LST -- what is your target canopy? If you have no idea what the end goal is, any training you do is whimsical in nature. Have a plan. That will answer most of your questions on its own. You do what is necessary to reach the number of colas per sq ft that plant is responsible for. If it's an auto, i wouldn't do much training as it really doesn't help much. Spreading out an auto to avoid congestion and maximize light absorption is as good as it gets.
3 colas per sq foot is all you need. A little less or more due to math of number of plants and area of coverage is no big deal. Even 2 colas per sq foot will maximize yield in many cases. The only time you'd want more is those rare plants that stretch like a mf'er and only produce smaller bud sites. Tend to get a deeper zone of decent buds, but still need more branches per sq ft to get a decent yield. OVerall, the garden should give you 50g/sq foot or more... less if you have a herm or weak plant, etc. If you got a room full of killers, yo might hit 70g/sq ft with ambient co2. Tracking yield will help you assess your methods. When growing from seed, you need to consider genetic diversity, too. Any one plant not up to snuff hurts these metrics significantly. But, when you make adjustments and see it generally trend upward, you know you made good choices... or vice versa if results trend down. No biggie if oyu don't hit it when you start growing, but it's achievable with limited experience if you avoid bro science.