Coming along nicely now, some weight starting to be added to the buds. Leaves are quite frosty. Smells very sweet like candy.

Indoors flowering nicely, brown hairs starting to show. Outdoors still one of the Ultimate still hasn't started to flower. I'm starting to wonder if it's not an auto at all. The gelato is forming nicely, with a little visitor a bee. The other ultimate is also flowering nicely. Day 75, due to hothouse damage the three outdoor plants are now indoors

Everything going good just have to get these humidity shifts under control other than that no complaints this week I love growing wish I could do this for a living 💚

Really good week but the plant is still stretching. It really needs to stop otherwise I expect problems. Really loving the extra feeds made possible by the automatic watering, which will really be tested this week as I'm away for work. If anything goes wrong nothing can be done to rectify until Saturday. Fingers crossed it's smooth sailing anyhow. Bud sites a plenty anyhow, just hoping they continue to grow and finish off fat and juicy.

Tag 58: Die Blätter der beiden Pflanzen sehen zwar nicht so gut aus, aber die Blüten um so besser!😍

Welcome back to yet another update on my summer grow 😎🤙🏻 Things are still going smooth, a bit defoliation and nematodes was used and hopefully they will do their job in a few days😁 Started to add bloom to the mix, so let’s see how things turn out. As you can see the red spectrum is really working well, and soon I´ll add some small amounts of IR/UV daily. Looking forward to see the result 😊 _____________________________________________________________________________________________ SpectrumX LED🔥 @medicgrow 880 Watt⚡️ 2x UV/IR LED panels 💥 2.7 μmol/J🔥 Full Spectrum V1,F1,VS,FS🌈 Display with PPFD & Live spectrum Light measurement: Apogee MQ-610 & Apogee DLI-600. Fertiliser: Organics Nutrients https://www.organicsnutrients.com/en/ Green Buzz Nutrients Discount Code: GD42025 Grants 25% with a minimum Order value at 75 Euro. https://greenbuzzliquids.com/en/shop/

🌱Day 36. Start flowering.💚 🌱Day 38. Keep watering with clear water. Super soil working well.💚 🌱Day 40.💚 🌱Day 42.💚

Ultima semana de crecimiento en la que seguimos sin aplicar ningún fertilizante y hemos ido reduciendo las horas ya mañana la dejamos en 12/12 y cambiamos a periodo de floración. Saludos y buenos humos!

Harvested the Clones! I'm stoked how #4 grew out, I think she's the winner! With limited space in my grow, I'm only going to be able to bloom out the rest of the phenos clones to week 3-4.. The show must go on.. It's the price I must pay for using non-ideal cloning methods... Next round of phenohunting will have 100% cloning success, using my new aeroponics cloning machine.

🎩🌈🍬

Día 40 me ha dado carencias de calcio magnesio y posforo y potasio, me he dado cuenta un poco tarde... Las hojas estaban caídas y se me estaban poniendo amarillas con manchas marrones...les puse calmag y empezaron a buscar la luz ... También me di cuenta, que las Lili que no veía se me ponían las hojas moradas, creía que era exceso de nitrógeno, pero no era posforo y potasio... Así, que lo que no quería hacer, lo he hecho he metido a tope de nutrientes y más... Espero no liarla más...

With one away I thank god for the space as you will see from pics and videos the one on the right doesn’t look so great but hopefully with the space thinks will get better onto next week ✌️🏻

Buenas a tod@s... Bueno otra semanita más de estas hermosas nenas, ya más grandes y empezando a desarrollar sus frutos, la verdad q están creciendo muy bien, sin problemas, aunq hay veces q la temperatura este un poco alta las voy controlando y van muy bien, este cultivo yo creo q promete para bien... Me gusta ver buenos resultados cada vez q empiezo un cultivo nuevo, super contento y las niñas tanbm... 🙌🏻🙏🏻💀😎💪🏻 Buenos humos para tod@s...🔥🔥💨💨 🇦🇷🤝🏻🇪🇦

Flowering day 42 since time change to 12/12 h . Hey guys :-) A lot happened this week :-). The buds develop really nicely . The scent wafts through the whole room when I open the tent 💚. This week was poured 3 times with 1 l each (nutrients see table above) This week there was the maximum amount of fertilizer, which will be slowly reduced from next week :-) . Have fun with the update and stay healthy 🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : Clearwater Seeds Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany

Deep Forest Auto is still moving along. She is still bulking. She has started growing faster with the ph fixed. Starting to see a brow pistils here and there. I can do nothing, but wait. Thank you Doctor's Choice, Gen1:11, and Medic Grow. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g.

Room continues to pump out growth. HEat and humidity were an issue this week, but was managed with multiple garden visits and some air diversion. Extremely impresed with all genetics in the garden and there has not been any growth issues to date. 5 weeks complete, another 5 more to smoke. Thanks for following. See GreenMachine's diaries to see how to really grow In House Genetics

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.