Day 27- day 1 flower Flip to 12-12 Day 28-day 2 flower Calmag -0.8ml Coco A - 1.5 Coco B- 1.5 Roots -0.2 Day 29-Day 3 flower Calmag -0.8ml Coco A - 2.5 Coco B- 2.5 Active boost -2ml Roots -0.2 Day 30- Day 4 Watered Coco A - 2.5 Coco B- 2.5 Active boost -2ml Roots -0.2 Day 31–day 5 Watered Day 32– day 6 Watered Day33- day 7 Watered Day 34-8 Watered last night so nothing today Day35–9 Watered Day 36-10 Watered Day 37-11 No feed, Day 38–12 Fed 1.5L Day 39-13 Watered Day40-14 Fed pk Day 41-15 Fed 1.7 coco a 1.7 coco b 2 ml boost 1 ml Pk Day 42-16- started adding pk Fed Day 43-17 Fed Day 44-18 Fed 600 ml just before lights out 1L lights on Day 45-19 Fed 1.6 L Day 46-20 Fed 1.5L Day 47-21 Fed 1.5L Day48-22 signs of potassium deficiency so keeping the food levels up for few more days , started with light feeding but now doing heavy feed chart . -Starting to give off a berry and gas smell . Day48-23 Fed 2L She’s thirsty today . Usually start to see runoff after 1.2 Lbur nothing Ml/L Watered 2.5 coco a 2.5 cocoa 2 sumo boost 2ml by accident instead of 1 Day 49-24 Fed 1.5L Day 50-25 Skipped feed to try lower humidity Day 51 Fed 2 L but no run off . Think she’s thirsty from no water yesterday . Will do another 500 ml to see run off if not I’ll leave it at that .

Day 36 flowering: This lady is my superstar. her growthbhas been excellent and never slowed at any point. Her buds are solid and orangey when pressed. Size wise she is huge at 2ft from the pot top. 5 weeks left for her and i know she is going to be one of my personal bests. A beautiful Keeper strain so far.

Welcome back to yet another update on my summer grow 😎🤙🏻 Things are still going smooth, a bit defoliation and nematodes was used and hopefully they will do their job in a few days😁 Started to add bloom to the mix, so let’s see how things turn out. As you can see the red spectrum is really working well, and soon I´ll add some small amounts of IR/UV daily. Looking forward to see the result 😊 _____________________________________________________________________________________________ SpectrumX LED🔥 @medicgrow 880 Watt⚡️ 2x UV/IR LED panels 💥 2.7 μmol/J🔥 Full Spectrum V1,F1,VS,FS🌈 Display with PPFD & Live spectrum Light measurement: Apogee MQ-610 & Apogee DLI-600. Fertiliser: Organics Nutrients https://www.organicsnutrients.com/en/ Green Buzz Nutrients Discount Code: GD42025 Grants 25% with a minimum Order value at 75 Euro. https://greenbuzzliquids.com/en/shop/

Stretch 12 centimètres Jour52 légère defolliation et arrosage avec deux litres d'eau PH 6.3

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Big girls. Usually don’t let them get this big. They are starting to yellow a bit on the bottom. Not enough water me thinks

Forgot to record last week (due to being too high) but here’s week 4 they seem to be doing great😄.

Ernte. 3 Wochen Fermentation/Curing. 62% RH mit Regulierung über Boveda Hygro-Pack 62%

She is so tall...cant raise my cob anymore! Lots of sites...smells stanky!! In a good way...sort of.

it's too hot, buds could be fatter... we,ll see what happens towards the final weeks. Day 69, foto 1. Caramelo upside down 🙃. The main stem still standing up. (see foto 2, day 69)

Watered and fed on 19.1.2024 and dli around 45 She’s definitely stretching and is growing a lot day by day . Overall since last feeding she’s overperforming again and I hope I don’t run out of tent soon 😂 Overall she’s doing good and I have to adjust the light on a daily basis to stay on her heels . But damn she’s going all out for me it looks like 😍. Starting to smell a little like some kind of pineapple/skunk kind of way but not unpleasant at all rather very pleasant so far . It looks like she’s about to develop some big buds and I’m ready for the journey .

This week it's all about the 8 White Widow clones, rooted well in shallow water bath with .5ml pH perfect Grow in the water. Long roots sprouted through the rockwool and they didn't seem to be inhibited by the 50w led. Now transplanted into their 1L pots with fresh soil substrate, no additives. Transplanted 2 clones into rockwool now in 1 pot, see how they (Cerberus) develops. Harvested the clone cuttings on 24/8/2020, took approx just over 2 weeks (17 days) to develop the long roots seen in the pics above. Kept the water feed fresh every 3/4 days to prevent algae build up. Switched to 12/12 11/9/2020 Unfortunately, I had to pull this grow, it was painful to ditch the plants as the grow was going well but i had to move house and couldn't take them with me.

From indoor to outdoor :)

Stretch hört langsam auf - leider bissl zu spät obersten Buds werden zu nah an der Haupt-led (klebt bereits an der Decke mit Gurten) sein - das ist mist. RH war diese Woche mist - konnte es einfach nicht runterbekommen (trotz starker Bechneidung) und somit keine hohe VPD fahren. Durst und Transpiration waren enorm. Weiter gehts mit Sativa Langabenteuer

I'm really happy with the gas tax x obama runtz plant is doing. It started out so small, I wasn't sure if it was gonna make it. But lately it's taken off. The leaves are a lot bigger and the colors are so vibrant. I can tell it's happy and healthy now. Well, it's Christmas again. This year feels a little different, though. Maybe it's the snow blanketing everything, or the way the tree lights up the whole living room. Either way, it's cozy and warm, even when it's freezing outside. I'm really looking forward to seeing what Santa brings. I hope I get that new video game I've been wanting. But most of all, I can't wait to spend time with my family. We always have so much fun This past week was a good one for the plants. They started the first week of their stretch, and they've already grown a decent amount. I can really see them filling out. It's cool to watch them grow so fast. I can't wait to see how big they get by the end I always get a little worried at this stage, like they're not gonna get as big as I want them to. But then I remember how they always end up surprising me. By the end, they're always perfect.

Good news everyone, we reached week 6 and everything is going smoothly. I did add some trellis rings to keep her spread out and the automated will probably be set up tomorrow. 11.07. My pump is weak, but water is flowing. Did add some shelving to make sure the hydro halo is not siphoning the reservoir. At the moment I am watering for two minutes every 12 hours, will tune the interval over the next few days. I did stop the foliar feeding for now. 14.07. The watering interval is down to 90 seconds every 12 hours, I still need a stronger pump, it is working but the halo should splash out way more water and all the substrate should be rinsed, room for improvement. 15.07. not a lot happened today, the watering interval seems to be okay, will probably add some more support braces, just to have done something. 16.07. Did nothing, I am occupied with renovating our house.

Pushed EC a bit this week. EC Target 1300-1600 1730 1679 1637 1831 1852 1991 Things is growing well. Bud nodes are tight and getting hairy!

Week 5 flower going well. Nothing much to report. Increased the drip as she is taking in more water. Turned up the fans to 100% for maximum flow, and due to that, the tent got a bit warmer and a bit dryer so the exhaust fan and humidifier are working a bit harder. 75-77 deg F, 50-60% RH. Not sure if the lights are to intense or if it’s purple coming through, but at the highest tops there seems to be a bit of discolouration on the newest growth, just at the tips of the new buds. But I’m looking really close and it may be purple, not brown. I’ll keep an eye on it and turn the lights down if symptoms worsen. This plant is growing strong and getting chunky. Hopefully she keeps growing well until the end. Thanks for the views…keep calm and grow on!

07-09-30 Esta semana no se ha rectificado PH, pero al 7 día no había agua a tenido que tirar de reservas. Ya están las 3 floración pero se nota que la planta que se hizo apical florece antes y más uniforme. Se cambia de solución y se lava las raíces. Se baja 0.2 ms y PH 6.5. Luego se vuelve alimentar con una solución como hay en los datos de esta semana. 09-09-20 Conduc: 1.92 ms PH: 6.05 Temp: 24 °C 10-08-20 Conduc: 2.23 ms PH: 6.15 Temp: 25 °C