Skipped a week so that the weeks in the diary were the same age as the new plants also took over my wife's aquaponics ak auto flower that is 4 weeks old so ill add that into the grow. The aquaponics plant was in rough shape it was showing huge signs of cal mag def. So I went to the local fish store and bought a cal mag substitute that's safe for fish also dropped the ph to 6.1 she had it at 7+ (not her fault ph metre broke )now plant looks great 1 week later after pulling off all affected leaves. Nothing new with the girl sco8t cookies except I fed today 1 gsc starting to lighting up

Start of week 3 of flower! 😍😜 She is looking fabulous! Pistols are starting to swell 🌲🌲🌲🌲 Started bIg bud coco and also added some BOOST Not applied anything defiolation or neither any training All the top are uniform without any training -------- -------- -------- Leave a like and comment below on your thoughts If you want to support us, take a look at our new diaries and if you like them remember to like, comment and follow us 👍 It will be appreciated! ❤️ Join our community https://discord.gg/2kXxgHTaCZ Thank you very much! 💪 🙏

Heute früh ist mir ein sehr unschönes Blatt und eine graubraune Stelle aufgefallen🙈 und was soll ich sagen der Headbud schimmelt🙈 also hab ich gleich ne Schere Genommen und großzügig abgeschnitten

Siamo all'ultima settimana.il flush è iniziato da un paio di giorni💪👍🕉️10/15 giorni ancora e via......in questi ultimi giorni sta buttando fuori dei profumi intensi e vedo che sta tirando fuori dei colori favolosi. ... non vedo l'ora di assaggiarla Pre sentire se funziona bene come terapia😉💪💚💛👍

Day 78. Girls is down. Dry trim chosen as to most smaller plants. Overall I am happy with Urban Legend brand, only Gorilla will be meh.... ;))) Day 88. Girl went to jars, amazing 62 g of her !!! Top quality all the plant through. We are testing it with my friend's wife and it's pure joy, think after cure I'll keep it pure for myself, no share ;))) Happy Growing !!!

OK guys I was away for a week so this week we are at the end of week 4 veg instead of the beginning of the week. There was a boom growth period and the taller Punch 2 has bent herself to avoid getting burnt as she is 30cm tall, the other Punch 1 is 20cm tall and seems fine. I have just given them a watering with calmag and just a drop of PK ratio 13-14. I have also ramped up the lamp to 100% and put it at 55cm from the top of the plants. I'm hoping they will soon switch to flower guys fingers crossed. 🎄

Nice growth and bud developtment. Hps still doing its thing while im sitting back and relaxing. Got to keep an hawk eye on watering. I feed full strenght going over to full canna so they were transplanted into soil. Clones doing good but the Lemon skunk clone dont feel to good. Lets see what the week will bring.

SO COOL! These plants are so remarkably tolerant!! They look so cool and are always a hit with guests. Every other watering I just blast the hell out of the root zone lmao Wish the perlite wasn’t there sometimes to help with the effect - or maybe rather I need some gravel to sell the effect? 🤔

Not sure if I did this LST training correctly, we’ll see. I’ve been following the fox farms 12 nutrient chart religiously along with their mackerel and Kelp me and they seem to be enjoying it. I’m noticing more yellowing than I’d prefer so I ordered some cal mag on Amazon which should be arriving soon.

Esa familia, ya estamos de vuelta, y es que me sorprende en primer lugar la intensidad de luz y lo bien que crece cada ejemplar, no da casi calor así que para el verano irá de lujo. Nuestras candi caramelo acaban de terminar su 3 semana y es que pronto trasplantaremos a macetas de 7L, controlamos la temperatura, y la humedad la tenemos algo baja pero sin problema alguno, estás candy caramelo de Zambezaseeds están comportándose muy bien en interior, pronto cambiaremos el ciclo a 12/12 , en nada pediremos la tierra y al lío. Www.mars-hydro.com web: http://bit.ly/2uJAjgy ts600: http://bit.ly/3cnv0Ev code: an420 La semana que viene más y mejor buenos humos fumetillas

Enjoy the queen 👑🌳💚🇯🇲☺️

Dear friends, I wish you a Merry Christmas and a Happy New Year! I can’t say that something more important happened to me this week than your time spent with your family)) But I’ll tell you a couple of things! Firstly, I set up a camera and recorded timelapses, enjoy)) Secondly, I started new pallets while both bushes are on the same, but will stand on different ones when they grow large. In third place this week, but the most important is automatic watering. One pump waters 2 plants with a solution at once, watering occurs evenly at 1100 ml per minute per plant. At this stage, I water the plant 4 times a day, 300 ml each. 700-800ppm. pH 5.8 is strictly observed. In fourth place is the news that I cut off the tops, after that I will try to bend them in different directions before installing the scrog mesh. Next week we will install a scrog net, a co2 tank, an automatic co2 supply system. By the way, I forgot to tell you, all growbox systems work from the application on the phone: watering, nutrient solution, fans, and we will add co2. next week. In the meantime, friends, I wish you happiness and great harvests in the new year! I love everyone!

Here we go. Week 8. I’d say my best grow so far. Girls are stretching as expected, they have plenty of room so not issue. There are plenty of buds forming so I can’t wait how this grown turns out. :)

Gracias al equipo de AnesiaSeeds, Marshydro, XpertNutrients y Trolmaster sin ellos esto no sería posible. 💐🤯 39%Thc Wham Boom: Sube al ring con WHAM BOOM de Anesia Seeds, donde la energía electrizante de Wham choca con las vibraciones frías de RS54, creando una variedad que es todo equilibrio, potencia y sabor. Este peso pesado feminizado ofrece una mezcla perfecta de 50% Sativa y 50% Indica, proporcionando una experiencia que te hará flotar de euforia mientras tu cuerpo se sumerge en una serena relajación. WHAM BOOM es una productora prolífica, con rendimientos en interior de 600g/m² y en exterior que alcanzan la asombrosa cifra de 900 - 1200g por planta. Con una floración de entre 65 y 70 días, esta variedad está lista a principios de octubre, lo que la convierte en una competidora ideal para los cultivadores que aspiran a una cosecha de campeonato. Con una imponente presencia de 100-140 cm en interior y 160-220 cm en exterior, WHAM BOOM se alza como testimonio de su destreza genética. 🏠 : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit 🌬️💨 Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ 💻 Trolmaster Tent-X TCS-1 como controlador de luz, optimiza tu cultivo con la última tecnología del mercado, desde donde puedes controlar todos los parametros. https://www.trolmaster.com/Products/Details/TCS-1 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 2: Fantástica semana, estan creciendo de una forma espectacular gracias a la ayuda de @marshydro, @xpertnutrients y @trolmaster. Se puede apreciar un tercer nudo aunque la aplicación de cola de caballo y el estar en una maceta pequeña ha influido en el tamaño de ella, parece una planta algo sensible,se recupera muy rápido 😁. Esta semana espero trasplantar a la maceta definitiva. Potencia de la lámpara: 50%

Toutes les 5 ont germées, 2 moyennes banana+ 1 grosse .(racine) 1 moyenne purple bud +1 grosse et une très petite (racine) Elles ont germées toutes en 3 jours. Puis je l'ai ai mise dans des pastilles de coco et chaque tourbe dans des petits pots en plastique, sur un plateau métallique devant ma fenêtre (je n'avais pas encore de box) du 2 mars au 7 mars.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.