2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Now we are in week 8. Plants stopped growing. I will let them grow two more weeks and then I will at least harvest two of them. Depending on the trichomes I will keep the other two for 4-5 weeks more, because I will be away ... traveling lets hope that the sun is coming out again and that the buds get bigger and bigger in the last two weeks. BTW I stopped giving any type of nutrients, just water.

LIMETTA MARMALADE by JOLLY POND FARM (Cotton Candy Kush / Mimosa) x (Koffee Cake / SkyCuddle Kush F3) Bred by @landrace_bureau Hunted/Selected/S1 by Jolly Pond Farm @jollypondfarm jollypondfarmacy.com Sativa Flowering Time: 68-73 Days ~~~~~ 1/14/2025 res were all swapped yesterday except for #3 , roots barely reaching through, still top watering, as soon as i see its 5 finger leafset, i'll switch to veg nutes. VPD @ 0.9kpa ish DLI @ 30 ~ ~~~~~ 1/21/25 i have noticed that the limetta marmalade are a litttle more sensitive to light at this stage than most of what i am rocking, but that could be because i've gave them a little light to begin , so i can't say for certain. however everything is looking good. Follow me on Instagram @growmoregaas & Twitter @growmoregas

Smells just like the chocolope i know.Love this strain but i hate the fact its so PM prone as ive started seeing some spots on her.Pretty pissed but ima try keep it under controls for the next 2/3 weeks.I might even chop the main cola as its starting to look ready

She's looking very healthy and ready to give all of her, looks beautiful,let's see how she performs the rest of the way! 💚🌱💎🔝

Gold Sativa is doing well. She is bulking now. Has a nice frost. She got a solution change as usual and ready to go for a few days. She is in a New Level Hydro bucket, under a Spider Farmer SE5000 light. Thank you Spider Farmer, New Level Hydro, and Quebec Seeds🤜🤛🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g SE5000 https://amzn.to/3qFpAML Spider Farmer Official Website Links: US&Worldwide: https://www.spider-farmer.com UK: https://spiderfarmer.co.uk CA: https://spiderfarmer.ca EU: https://spiderfarmer.eu AU: https://spiderfarmer.com.au Coupon Code: saveurcash Www.newlevelhydro.com Www.hygrozyme.com

Temps at 78-84 f, humidity inside dome around 68-76 depending on temps. Direct planting into coco jiffy plugs, charged with a light seaweed extract. Took 2 days exactly for seed to pop out of plugs. Another day to remove its shell, will start counting as day 1 from here on out. Check out my other diaries for the other strains. 1 gelat.og auto (seedsman) and 2 extreme impact auto (heavyweight seeds)

GreenHouse seeds è sempre una garanzia, super lemon haze più volte vincitrice di premi non delude mai, una pianta fantastica

Veg box is starting to flourish. Both lemon and sativa clone are very bushy and healthy. All others are healthy as well, and will be topping these soon. Once they start shooting again they’ll be transplanted once more and thrown into flower to start my first “sog” grow. Two ladies in flower are well. One still showing burnt and curled tips and I think flower growth is slowing down. The other is happy and well. There were two babies out in flower last week, one turned male. The other is happy and healthy. Until next week...

Chopped on day 133 - trichomes look swollen and ready - leaves faded out. Cut this according to the biodynamic moon gardening calendar and the dates did seem to align with the plant aromatics being more noticeable. Very excited to do the smoke report on this one - focusing on 60% humidity @ 60 degrees for 21 days to retain terpenes and quality.

Balanced compare tea given this week, but nothing else amendment wise and no other watering needed still. Nice stretch gone on in the first week of flowering, plant still looking nice and healthy, will see how things are looking for a defol on day 14 or so.

Vamos familia, actualizamos la octava semana de floración de estas Black Rainbow de Seedstockers, salieron las 3 de 3, 100% ratio éxito. Aplicamos varios productos de Agrobeta, que son increíbles para aportar una buena alimentación a las plantas. Temperatura y humedad dentro de los rangos correctos dentro de la etapa de floración. La tierra utilizada es al mix top crop, por cambiar. De 3 ejemplares seleccioné los 2 mejores para completar el indoor y trasplanté directamente a macetas de 7 litros, cambien el fotoperiodo a 12/12 y aplique una buena poda de ramas bajas, se ven bien sanas las plantas, tienen un buen color y progresan a muy buen ritmo por el momento, cogieron una tricomada seria. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

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Week 4 My girl reaches her final size. This was the plant that suffered less from unbalanced soil, the damage is almost imperceptible. I took the opportunity to put the scrog that will be more to support the buds weight. Despite the initial deformity of the leaves, the plant has a very healthy aspect and a very balanced coloring, so I believe that this adventure will not influence the flavor of the flowers. In few weeks... HARVEST TIME 😋 Day 22 Hard defoliation on fan leaves.

Les filles ce sont pas mal sortie de cette canicule, certaine ont souffert d'autre pas du tout.C'est partie pour la Flo.

Alguna ya esta ready para meter las ✂️ y las demas .. será cosa de días 🙊🙊🙊

This week the Chicken n' Wafflez continued to bulk up steadily. Buds gained noticeable size and density while resin production increased across the entire plant. The sugar leaves are now heavily coated in trichomes, giving the flowers a very frosty appearance. Environment: 25-26°C • ~60% RH 🌡️ Feeding: Water, CalMag & 1x Orgatrex The aroma became more complex this week. Alongside the sweet fruity and jam-like notes, a more herbal and spicy character started to emerge, adding extra depth to the terpene profile. 🍓🌿 Water consumption remained stable throughout the week and the plant showed no signs of stress. The foliage stayed healthy and green while the flowers continued stacking along the main cola and side branches. Feeding was kept simple with plain water, CalMag and one application of Orgatrex. 💧 Overall, this week felt like a transition into the final stages of flower development. Buds are swelling nicely, resin production is ramping up, and the terpene profile is becoming richer with every passing day. ❄️🔥

Day 28 week 4 of flower plants looking good I dropped a 3L water bottle on a string which snapped a whole branch from the bottom 😳 on right plant 😭

Shes chunky as nugs are solid shes got a nice fade leaves dropping and turning yellow been in flush since 17/11 ran 8l through her and will be watering with 2ml of terpinator to 1l water at 2l per day for the next 10/14days she smells really sweet a little earthy aswell really pungent now trichs are near enough all cloudy with some ambers should be nice by the time flushing ends . tied a few of the top buds up for support during flush will get some better pics first plant in the video is the cut the next is 2 gorilla then blue mystic two further to the right are gsc and another cut last one in the vid is the critical thought id put a little vid of the whole room thanks for reading happy growing guys