08/30/2021 - Noticing more gnats than usual. Could be overwatering, anaerobic soil conditions, or both. - Going to set up fly traps, starve both the plant and the gnats of water for about a week. - Will observe any stress responses due to an extended dry period.

did this beautiful girl a topping, depilation, and LST, she feels good💚

She's in flush, bulking up and smelling citrusy

week intel: we getting close to end of ripening , this week i raised drought , Nitrogen and E.C stress by feeding them 2 times per week and raised e.c to 3 to cause real stress that will help increase terpenes production stresses : Nitrogen deficiency Stress by removing the fertilizers that has N in them Drought stress via removing one meal in week high E.C stress around 1.7 and the other feeding day 3.0 , 2 times a week feeding: no more Nitrogen i feed them 2 times this week with this order : day 1 : i feed them high with Bio-Bizz Top-Max + Feeding Booster about 850 ppm - 1.7 e.c to cause a medium e.c stress. day 3 : no more feeding this day day 5 : i feed them very high dose of Bio-Bizz Top-Max + Feeding Booster around 1500 ppm - 3 e.c to cause high e.c stress guide of the week : be patient in the last weeks, never hurry for harvest because its the time of ripening in last 2 weeks you should let plants to do their work last weeks of ripening is the time to break the limits we should cause 3 stresses as i said above Nitrogen stress , Drought Stress , E.C Stress

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

February 20, 2019 update: The good news is the babies are all growing, the not so good news is there seems to be a slight bug infestation. Since taking these pics and video I have sprayed them with a mild Neem Oil & Water mix (about 20:1), hoping that helps. I also have a few clones looking a little funky, but that's OK with me, out of these 16 babies I really only have room for 9-12 in my flowering area and 12 would be pushing it. The MediKush & DinaMed seedlings are doing great. DinaMed's are still taller, but the MediKush has some beautiful tight nodes.

Raising up this solo cup

The gelato 45 is becoming enough big 🤓💚

Hands down she has raced to be the first plant that will be harvested, which I do not mind because this cheese scent she has is driving me crazy, my senses are drooling 🤤 and crying, patience is a skill reserved for the brave. Love how the LST has given me about 3 main stems, the crystallisation on this plant is incredible, it looks like I am growing it in an indoor environment. All she’s doing is fattening her buds, I hope she gives about 50grams of dry bud. I need to start formulating a questionnaire, I am going to dedicate 10grams to researching how well I grow bud, that is six strains, 10grams each, that is about 60 joints weighing a gram each. At the end I want to open my own community weed forum.

exelentw

01/27/25. Welcome to the start of week 4. Well, fatso is doing great now. She’s perky and happy.. AND she’s darkened up quite a bit. No more pale girl. She was just a hungry little hippo 😸😸 She was just watered yesterday, slowly, until a bit of run off. I’ve upped her food to 2.5 across 3 parts, as well as upped calmag (1) and silica (2). I pulled a dip shit move and left my measuring tape in the flower tent and it’s lights off, so I’ll have to come back tomorrow to update that. She’s at 4 nodes and as soon as she’s got 5 I’ll be topping her. Update: 1/29/25 got measurement updated and watered with food. 1/31/25- topped and plain watered and added picture/video Thanks for lookin. ✌️

Jetzt war für mich der optimale Zeitpunkt zum toppen. Sie hat es gut verkraftet, was man auf folgenden Fotos sehen wird. Ich habe versucht so gut wie möglich im 45 Grad Winkel zu schneiden. Aus allen Cuts mache ich aktuell Clones. Ich liebe Clones aus den Main Cuts. Daraus mache ich dann Mutterpflanzen. Die Mutterpflanzen von den besten Phenos bleiben.

While I was away in Mallorca, enjoying the sun and smoking some Sativa Landrace, the girls were given a more simple feed to make things easier for my friend whilst he was taking care of the garden. The feedings came to around 700ppm (Including 150-190ppm tap water). As you can see in the video I've been bending her down for a couple of days instead of tying a string to her. It's just a lazy LST that should basically do the same thing. This Royal Cookies girl has progressed well since her topping and LST. She's a bit stretchy but a few more toppings should make her bush nicely. Every second feed from here on out will also contain Mammoth P for the first time in my garden! 🐺

Total was 10 and 1/2 weeks. Final week was good and filled out a little more. it looked to get a little more sticky. Trichomes started turning Amber last week that's just how I wanted it. Smells is toxic and sweet if that's even possible. All in all, a great little plant, I would like to see it go free in a larger space, I bet the yields would be amazing!!

I'm still struggling with that temperature. unfortunately, the substrate once dried up for me.

Beautiful pair of phenos of Badaz og cheese very excited about what strain is gonna offer me in terms of quality buds, so excited to see what is every of this 2 phenos gonna be like, I really hope that both of them have the exact terpenes profile and the same potency! Let's keep on working! We'll the ladies have been Transplanted on February 2nd after 17 days since planted, they were very big and the pot was completely conquered by strong roots as you can see, now both of them are in their new 11l pot home let's see how they keep developing! 💛❤️💚🔝💎

17 sept. Please let there be sunshine, Mould had been spotted mainly in the northern part of the plant. Rain had been pouring down for nearly a week and air humidity was around 80-90 % Plants are way to far in their flower cycle for these kind of showers and you can see it has taken its toll. Pistils are turning brown and buds need to be checked every other day to stop mold from spreading. She still needs a week more maybe 2 if weather allows it, but she will be chopped before the next rainy days. I will lose a part of the harvest to mould weekly but for me the maturation of the harvest is more important than the quantitity. As you can see there are plenty of buds left that still have chance of maturing. Harvesting now would be a shame. Simply hoping for some dry warm days for her to swell and put on some weight.

HOLY SMOKES!! Y’all seeing this?? Look how fast they are growing!! Wow 🤩 I’m so impressed both with the light and the superponics!! The light is giving perfect node spacing and the superponics is speeding up the growth 💯.. so what I been doing is 4 days lotus nutrients than I empty out the res and add hygrozymes and hyclean. Both by the same compmany! Both helps with salt build up and cleaning the roots/res… Plz follow my YouTube channel (Familytrees19)