10/10 would grow again.

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

6.12.25. 6th week flower started on 6.9. Gorilla zkittles still looking like a Frankenstein after being stunted by tying her down and being way stressed out! Still growing away though putting on bud but this is the smallest plant i have ever grown! Little disappointing but can’t wait for next grow! I’ll never stop growing been going strong for 15 years! Thanks for checking out grow I appreciate it!

Hello everyone week 2 of flower has passed for this Mint Jelly auto ❄️ Mars hydro FC-E6500 75% have a great day and wish you all happy growing 😎👨‍🌾🏻

Fun little grow, but battled with WPM early on. I'm sure the flavors are buzz is similar to last grow of this strain.

Had some hair style and off to get fat lol

She has hit week 5 and I have to say she is looking healthy and green, the growth has been so rapid considering she had a rough start in that propgation box. My earlier concerns regarding a stump in growth has started to disappear as she now sits at 8 inches tall and now leaves are start to grow in the middle. Very happy with the current outcome ☺️

Cosmic Noodles — Week 4 Report (Draft Framework) 1. Week 4 Context (12/12 Cycle) • Plants are now well established structurally. • Stretch is active but controlled. • Environment remains stable and unchanged, which is clearly paying off. ⸻ 2. Training Progression (End of Week 3 → Week 4) End of Last Week • Introduced gentle lateral bending using light wire. • Goal: break apical dominance early without inducing stress. • Response: immediate redirection of growth, no negative reactions observed. This Week (Week 4) • Introduced metal clamps to reinforce and stabilize bends. • Purpose: • Hold branches closer to horizontal / toward the floor • Encourage more even stretch and node activation • Maintain structure without repeated manual adjustment • Training intensity remains controlled and intentional, not aggressive. 👉 Important note for education: This is guidance, not force. Plants are being shown where to grow, not pushed. ⸻ 3. Plant Response & Vigor • Overall health: Exceptional • Lush green coloration • Fast growth rate • No stress markers (no clawing, no discoloration, no leaf deformation) • Training response: • Trained plants are accelerating growth and showing earlier developmental cues. • Untrained / lightly trained plants remain relaxed and expressive. • Leaf management: • Only gentle leaf tucking • No defoliation at this stage • Inner node spacing remains excellent, so intervention is unnecessary. ⸻ 4. Pre-Flower Observations (Very Important) • The plant grown in the plastic recipient pot, which experienced: • A different early light behavior • Slight vegetative overlap • More active training ➡️ Is showing early pre-flower signs. This is not surprising and is actually educationally valuable: • Training + structural stress (positive stress) often accelerates maturity signals. • Slight differences in early light exposure can shift hormonal timing. Meanwhile: • 12/12 from seed plants • Still in a clean transitional phase • No visible pre-flower yet • Strong branching (notably under ~6 nodes with good lateral expression) • Internodal spacing is ideal • Development is uniform and calm This staggered response is normal and healthy, not a concern. ⸻ 5. Nutrition & Environment (Week 4) • Nutrition: • Same recipe • Same measurements • No changes introduced • Plants are responding beautifully, so stability is prioritized. • Flower nutrition: • Not yet introduced. • Decision point clearly defined: • Full transition only once the entire room enters pre-flower / flower. • If a few plants advance earlier, a localized, light flower feed patch may be applied — cautiously and intentionally. This shows discipline, which is what separates dialed grows from reactive ones. • Room temperatures: • Stable • Supporting vigorous metabolism without stress • No signs of heat or cold response in leaf posture ⸻ 6. Grower Philosophy (This Week’s Key Message) “Everything is the same because everything is working.” This is one of the strongest educational points you can make: • No chasing symptoms • No premature feeding changes • Letting genetics + environment express naturally ⸻ 7. Outlook for Week 5 • Expect wider pre-flower expression across the room. • Training effects will become more visible in canopy distribution. • Nutrition transition will be timed, not rushed. FOR DISCOUNT CODES AND MORE JUST FOLLOW THE LINK https://website.beacons.ai/dogdoctorofficial 📲 Don’t forget to Subscribe and follow me on Instagram and YouTube @DogDoctorOfficial for exclusive content, real-time updates, and behind-the-scenes magic. We’ve got so much more coming, including transplanting and all the amazing techniques that go along with it. You won’t want to miss it. GrowDiaries Journal: https://growdiaries.com/grower/dogdoctorofficial Instagram: https://www.instagram.com/dogdoctorofficial/ YouTube: https://www.youtube.com/@dogdoctorofficial ⸻ Explore the Gear that Powers My Grow If you’re curious about the tech I’m using, check out these links: 🔆 Lighting & Environmental Control • Future of Grow — Advanced LED lighting technology https://www.futureofgrow.com/ DISCOUNT CODE: DOG20 • Lumiflora — Under-canopy LED lighting https://lumiflorade.com/ • TrollMaster — Environmental controllers and automation gear (past collaboration) ⸻ Genetics • Zamnesia Seeds — Genetics used in this project https://www.zamnesia.com/ ⸻ 🌱 Soil, Substrates, Boosters & Root Support • Plagron — Substrates, bio mixes, and supportive products https://plagron.com/en/ ⸻ 🎒 Storage, Curing & Preservation • Grove Bags — Curing and storage solutions https://grovebags.com/ ⸻ 📸 Photography Equipment & Tools (Not sponsors, but part of my creative toolkit) • Sony A6700 • Sony full-frame macro lens + few more • Stacking photography workflow - learning • iPhone (for behind-the-scenes shots) We’ve got much more coming as we move through the grow cycles. Trust me, you won’t want to miss the next steps, let’s push the boundaries of indoor horticulture together! As always, this is shared for educational purposes, aiming to spread understanding and appreciation for this plant. Let’s celebrate it responsibly and continue to learn and grow together. With true love comes happiness. Always believe in yourself, and always do things expecting nothing and with an open heart. Be a giver, and the universe will give back in ways you could never imagine. 💚 Growers love to all 💚 📸 P.S. – The Eye Behind the Lens All photos in this diary (for now — except for the ones showing the camera, which I took with an iPhone) are taken with a Sony A6700 paired with a Sony full-frame macro lens and a few more. Photography is part of the story — it’s how we share the fine textures, the glow, and the quiet details that words can’t always capture. I’ve also started experimenting with photo stacking — a technique where multiple images, each taken at a slightly different focus point, are layered together to create one perfectly sharp image from front to back. It’s not digital enhancement or AI; it’s pure photography — a way to reveal the plant’s beauty in microscopic depth, from trichome to petal. You’ll even see a few shots of "ghost me" capturing the shots — camera, lens, setup — because every grow deserves not just to be cultivated, but documented like art. FOR DISCOUNT CODES AND MORE JUST FOLLOW THE LINK https://website.beacons.ai/dogdoctorofficial NEW DISCORD - Official Server Invite Link : https://discord.gg/ksjAkA5T74

The 3rd week was a big one all plants finished stretch period and it was becoming a little wild for a second. I moved 4 of the bigger girls into their own room with their own 1000w LED plus a shared 250w HPS in the center. I will be tying down branches to get flat canopy then each of the 4 will be pushed more towards the center for better light coverage do not mind the setup its not fully there but will be finished before the end of the week. In the main grow room there is a single 1000w HPS cool tube running at 100%. To remove the 4 bigger plants I had to cut the entire trellis off the canopy but will be placing a new layer this week as well. As for nutrients feedings well clearly from the size of these girls you can tell they have been eating. In about 2 weeks I will defoliate one last time and then its them vs. the world til havest. Happy Growing!

I flushed the 3 girls with approx. 30l each and of course stopped feeding them about 1 week ago. Therefore, as you can see in the pictures, the older leaves turn yellow. Most of trichomes are milky. We'll see when the girls are ready for the harvest. GG#4 No.1: 100 cm GG#4 No.2: 100 cm Runtz F1 Auto: 45 cm

Sunday, May 15. 2022 Plants really looking good and happy to me 😁. The roots are sucking much more water out of the pots than my previous grow and I am giving them a gallon every two days now. They wilted slightly a couple days this week because I didn't realise how much water they were using. Supplemented feed yesterday with Recharge and Cytokin, and foliar spray of same solution this morning. Both plants started showing pistils a couple weeks back but now both are full furred out. Plant #2, the one with whorled phyllotaxy, has red and white pistils; I am not sure of the significance of that, if any.

✌️🎃Thank you for checking my cultivation.

pistils start to turn brown maybe 2 more weeks before harvest. some topleaves show calmag issues because i gave too luch bloom booster 2 weeks ago looks wors then it is

Woche 2 der Blüte hat begonnen. Die Pflanzen haben in der letzten Woche noch einmal ordentlich an Grünmasse hervorgebracht. Außerdem wurde wieder jede der der Pflanzen mit dem BioEnhancer von GreenHouseFeeding gedüngt. Die Automatische Bewässerung ist auf 5x 5min, über den Tageszyklus verteilt, eingeschaltet. Auch ist immer mehr ein Geruch beim öffenen des Zeltes wahrzunehmen. Im Laufe der Woche wird wieder entlaubt. Sobald der größte Teil des streches abgeschlossen ist, kommt ein Netz ins Zelt.

V4, están creciendo rápido, desde este punto empezaré con una EC de riego más alta 1.5~1.8, hace 3 días que aparecieron las primeras hojas verdaderas, por lo cual la etapa de vegetación ha iniciado. V6, el día anterior tuvieron un riego con pH 6.2 y EC 1.7, se me pasó el agua y quedaron algo tristes, pero ya se recuperaron hoy, todas las stash presentan puntos de decoloración en las primeras hojas, las cookies están en perfecto estado. Están comenzando a salir raíces por el fondo de los vasos(algo que nunca me había pasado con otras genéticas) además se ven muy gruesas las raíces, están pidiendo transplante ya, en unos días serán transplantadas.

💜🤜 Purple Punch OG by Sweet Seed 🤛💜 🤜19.9 🤜20.9 🤜21.9 🤜22.9 🤜23.9 🤜24.9 🤜25.9 I haven't been motivated this week, but I'm ready to go again. New updates tomorrow for now these 😀 __________________________________________ ❓ Are you new to the world of the cultivation and don't know where to safely buy your seeds? 😮From SweetSeeds you can find award-winning, sweet, fast, fragrant, beautiful, and delicious genetics !!! ✅http: //bit.ly/SweetSeeds_ __________________________________________ 🌱Did you know that Green Buzz Liquids fertilizers are 100% vegan? A complete line of products ready to give the best to each of your plants! Visit the site and see my journals to see how they work 🦄 🤯And with the code "dreamit" you will immediately receive a 15% discount on your purchases ✅https://bit.ly/GreenBuzzLiquidsPro __________________________________________ 👀 Are you looking for a good lamp to start with? 👀 🌞Viparspectra has something more than the others, take a look at their site. ⏩ Use "GDVIP" for an extra discount or "ViparDreamIT" for an extra 5 %% discount 👀 Search for it on Amazon ✅Amazon US: https://amzn.to/30xSTVq ✅Amazon Canada: https://amzn.to/38udUVe ✅Viparspectra UE: bit.ly/ViparspectraUE ✅Viparspectra USA: bit.ly/ViparspectraUS ______________________________________________ 📷🥇 Follow the best photos on Instagram 🥇📷 https://www.instagram.com/dreamit420/ https://www.instagram.com/dreamit4200/ 🔻🔻Leave a comment with your opinion if you pass by here🔻🔻 🤟🦄💚 Thank you and enjoy the growth 💚🦄🤟

No hermi flowers from these two clones from a hermi.This grow has been hard to keep contained ive been bending branches for 4 weeks and it sill stretches 4to 6 inches every few days i am out of space under the lights its been a jack n been stalk grow

FBA2509-week 1 of Veg This week has been wild. Once the roots made a foundation the upper growth has been explosive the last few days. Really cool to watch and see the daily growth. I introduce calmag and grow nutrients this week starting at a 1/4 strength. All in all they've responded well showing no burn what so ever. Happy Growing