🌿 Kweekverslag – Amerikaanse Patriot (Weedseedsexpress) Opstelling: Mars Hydro 450W LED | 11L potten | Potgrond Duur: 8 bloeiweken --- 🌱 Overzicht groei (week 1–5) Ontkieming & start: Snel en consistent, sterke zaailingen Vegetatieve groei: Compacte, stevige planten met brede indica-bladeren Opleiding: LST en topping uitgevoerd voor veelvuldige kroon Voeding: Lichte Bio-Grow dosering, gezonde donkergroene kleur Resultaat: Krachtige, bossige planten – klaar voor bloei --- 🌸 Overzicht bloei (Week 1–8) Stretchfase: Eerste 2 weken bloei een nette rek, hoogte eind ±65–70 cm Topvorming: Vanaf week 3 snelle knopontwikkeling, compacte toppen Harsproductie: Vanaf week 5 schijnbaar trichomen, suikerblaadjes plakkerig Voeding: Bio-Bloom 2,5–3 ml/L + Top Max 1–1,5 ml/L Luchtvochtigheid: Rond 40%, geen schimmel van stress --- 💧 Doorspoelen en bloeien (week 7–8 bloei) Laatste 10 dagen gespoeld met water pH 6,3 Bladeren begonnen lichtgeel te kleuren → perfect rijping Toppen werden harder en geur intenser --- 🌾 Oogstresultaat Oogsttijd: Week 8 bloei Trichomen: 80% melkwit, 10% amber, 10% helder Droogtijd: 10 dagen op 20°C, 50% RV Cure: 3 weken in weckpotten Eindgewicht (droog): ±110–130 gram totaal (afhankelijk per plant) Structuur toppen: Compact, harsrijk Aroma: Zoet, kruidig ​​en skunky Smaak: Aards met lichte citrus en kruidige nasmaak Effect: ontspannend, euforisch en lang aanhoudend – typische indica-dominantie --- 🏆 Eindoordeel De American Patriot van Weedseedsexpress is een sterk, betrouwbaar soort met uitstekende structuur en indrukwekkende harsproductie. Ze reageert goed op training en voeding, blijft compact, en levert dichte, geurige toppen. Cijfer: ⭐ 9/10 Pluspunten: Sterke groei, compacte toppen, dikke harslaag Minpunten: Geur erg sterk tijdens bloei (filter noodzakelijk)

Hello! What's up? Today was the last day, we ended week 13 with success! Barely gave them any water, try to keep it to a minimum so they dry 'faster'. Super happy with this strain, beautiful buds, smell, sticky af and quite dense too. Was watching them day and night, every day to make sure I'd cut them in the right day. Having a microscope is really handy for this point, allowing to harvest in the desired state. Now I'll prepare the room for harvest, remove the reflector, bulbs, etc, and flip the box to a 'drying station'. They look ready to go for me, and after the pictures I chopped them down, and have around 2kg before trimming. That's the most I ever got out of a grow at once. Super amazed. Thank you very much @weedseedsexpress for being part of the contest, was really fun and a pleasure to grow such strong strain!

This week I started her LST and bent her top over as far as possible without snapping and locked her in place. I use soft wire "Gear Ties" sold in the hardware section of most places. I got mine at Home Depot. I highly recommend these for anyone serious about Lst. I kept her in this position for about 4 days before adjusting placement and pulling the main top back down below the lower branches. I have been maintaining the same feeding schedule "week 4 hydro" 3/4 str main nutrients, 1/2 str Open Seasame. She started showing preflowers on me even though it's been in an 18/6 light cycle. So that's why OS and week 4 feeding was opted. Even though the plant was technically 2 months old, she's only been fed and treated right for 2 weeks now. So week 7 from seed, I started at the week 3 schedule.

I don't have anything to say for this week. Time seems to be moving slowly as harvest approach. Im hopping they do fatten up a little more, but who knows...

5/4 I stupidly watered everything this morning. They were pretty much perfect weight for transplant......then I watered them. Oh well. If that's the worst mistake I make I'll be ecstatic. Went back over and found my 1 gal and 2 of those wierd slanty airports that tapers and holds like a gallon and a half and washed them. I mixed up 12 gallons of soil in a garbage bag and mixed it thoroughly with my gloved hands. It's mixed good. Looking at my plants in sadness thinking, "today could've been the day" and noticed a Special Kush of average size I didn't water that weighed in the 220's. I was happy. I filled the pit arpund a cup before removing it leaving the perfect hole. Mykos and water were poured in. Plant came out of the cup REALLY easy. Suprisingly easy. This wasn't totally dry. I was amazed it came out so easy so I kinda rolled it around looking at the roots. I started lowering it in the hole and I felt something break away but it webt DIRECTLY into the hole made for it. I tucked some soil around it but it was pretty tight from packing it around the empty cup. Didn't take a picture but the plant is fine. That was an average size plant too. So I may have to give some of the strains I popped later a little longer before I transplant. After everything's been transplanted I'll need to change lighting again. I think I'll open the window and use the hps and the l.e.d on the days and nights cThat's these girls won't be hardening off. I so wanna go plant those others but my intuition tells me to wait until morning and it's been serving me well. A couple can stay in cups longer. Not much longer but still. 5/5 Transplanted everything but two this morning into 1 gals with 1/2 happy frog half ocean forest. Mykos and water used during transplant. Found put the l.e.d was on for 24hrs for a while. I made sure the timer switch was ON but it was a different cord plugged in. Oh well. All transplants went perfect. I'm picking up an adapter for the outlet so I can plug the power strip into the timer and only use one timer. I had to hang the 150watt hps back up with the 1,000 watt equivelant 105 true watts. I hope this lighting setup works. Chemdog #4 and One MK Ultra will be transplanted later today or later in the morning. I also opened the shade to the south facing window. Shits getting real. EDÌT: I FINISHED TRANSPLANTING THE LAST TWO PLANTS AND PICKED UP THE ADAPTER SO I COULD HANG THE HPS NEXT TO THE L.E.D. THEY WERE FIBE WHILE I WAS GONE. EASIEST TRANSPLANTS EVER. THEY ALL LOOKED PERKY AND HAPPY SIX OR SEVEN HOURS POST TRANSPLANT. I OPENED THE CURTAIN TO (SOUTH FACING WINDOW) BUT I MADE SURE THE PLANTS WEREN'T IN THE DIRECT LIGHT. EVEN THOUGH IT WOULD PROBABLY BE FINE. LAST TIME I HAD SEEDLING IN DIRECT LIGHT. I'LL PLAY IT BY EAR BUT IM HAPPY. EDIT 2. Went back over to meditate by the stream and do my qigong. It's 75 and BEAUTIFUL today. The girls will be going out for a few hours on days like this. Anyway when I went in I was surprised to see everything looking nice and happy with praying leaves. Nothing is in direct sunlight but damn that window let's in a lot of light. 5/6 Girls LOVED the transplant! They seem to be all happy. No stress. Easiest transplant I've done. Leaves are praying upwards so I think the lights are approx tje right distance. They'll be going outside soon to harden off. It's been in the 70s but it's rainy now. I'll play it by ear according to weather. I'll continue to monitor lighting and I may lower the hps. 5/7 The soil was super dry when I went over this morning. Everything looked awesome though. I used about a half gallon watering. I don't want to water to run off. I want to keep the nutes IN the soil. It's also easier to fix an undet watered plant than an over watered one. It came out to about a half gaterade bottle on the biggest and half that on the smallest. I'll gradually increase water after I monitor the response. The water sat on the soil for quit a while so they definitely needed it. Looking good so far. 5/8 Soil lookeddry this morning. I think I'm going to up the water. Last time I dod seeds I gave like a pink. Some plants still had a little dampness where I watered that I could see. Others looked dry but happy. I gave the REAL dry bigger ones a small circle around the rootball. Just in case. I'm thinking of lowering the hps slightly so it's at least level with tje L.E.D. That window being open gives tons of light. If u moved them slightly left they'd get DIRECT sun most of the time. I'll be bringing them outside soon to start hardening them off. EDIT: WENT TO CHECK THE GIRLS AT 5 TO MAKE SURE THEY WERENT DRIED OUT. THESE GIRLS ARENT JUST GETTING BIGGER BY THE DAY THEYRE LOOKING BIGGER FROM MORNING TO NIGHT! ITS BEEN RAINING LIKE CRAZY BUT WE WILL HAVE GREAT WEATHER NEXT WEEK AND I'LL BE BRINGING THEM OUTSIDE. I'M SUPER HAPPY WITH GROWTH SO FAR. ILL PROBABLY HST (TOP/FIM) AT THE NEXT NODE. I WANT THEM TO SETTLE IN NICELY BEFORE STRESSING THEM. 5/9 I may have stupidly uploaded doubles of pictures from today. Soil LOOKED real dry. I gave the plants their first real watering. I still went light. Almost a gallon with all plants. I know many say I should be watering to runoff but I want to keep the nutes in the soul as long as I can I don't want to overwater. I always start out careful. Once they show they xan tolerate it I'll increase water next time. This weekend it will be raining but after that we are getting some real good weather and I'll he taking the girls out to begin hardening off. I also need to take a look and top/FIM the plants now that they are settled. Also need to wash grow bags and sanitize outside. 5/10 It's pouring out. Horrible night and morning but this is about cannabis. After the watering ALL the plants shot up in size! Substantially. Leaves are over the sides of containers. I think I'm somewhere close with the watering schedule. I may bring the hps light down a little. I also NEED to start HST. I need to FIM and/or top these girls. Unfortunately I've been dealing with medical problems of my wife so I haven't got to it. If I get some sleep I may do something this morning or in the afternoon.

7/21 330am MONDAY everyone is looking good. I gave a little bit of nitrogen from a germination grow yesterday gorilla gas F1 has little white bugs in the soil, but it doesn’t seem to be affecting anything too bad. I don’t want to spray anything. It will affect the outcome and make everything taste gross. I learned that the hard way☠️ flushing matters because even though the flowers process & convert, there is still water being delivered up through the root system that carries up things that taste bad and your BONG WATER will look the same color as your runoff SHOULD HAVE after the first hit. DONT DO THAT, DONT BE LIKE ME!👍👍 7/22 3:22 AM I got five Mike Tyson clones. They are not autos, but I think they are gelato. They are great. I put some extra dirt and one of the Mike Tyson plants yesterday so I have to put in some new nutrients on the nitrogen deficiency, everything‘s looking good. 👍👍. 7/22 1030pm......WELL WE KNOW WHAT DOESNT WORK...... I SCREWED UP WITH RAPID START. IT WILL CAUSE VERY SPECIFIC BURNS TO THE LEAF TIPS AND SIDES WHEN USED. I MAY BE USING TOO MUCH, 0.5ML SHOULD USE .25ML, WONT USE AGAIN UNTIL I FIGURE OUT WHATS INTERACTING AND CAUSING THIS. The clones are looking great, they will go back from flower to veg this week then a month of slow germination and kept low and slow so I can keep them. 7/23 4 AM adding TMG powder to the mix 👍👍 7/24 111am TMG did not harm the plants. The clones are progressing nicely. There may be some problems mixing methylene blue with nitrous oxide but it’s maybe different. There’s a combination of amino acids. I’m still trying to work out. Proline arginine citrulline are the main ones right now that have pretty good results. Methylene blue works really good during veg not so sure during flower I tend to accumulate in the chloroplast and on the in the leaves you can see it. It doesn’t affect the flavor or the taste. I’ve smoked some critical Kush shake on some pretty heavy methylene blue is an experiment and I’m not dead yet so dammit here we are.👍👍👍🎉🎉. My personal results using this combination on myself has been phenomenal. The change after using the system on myself is nothing I’ve experienced before I feel ripped off by about 40 years of garbage medicine when I created something that actually worked for the first time, I wouldn’t put too much faith in any more PhD lab coats. They are all failures and I found a system that works.👍. 845am PROP WALL UPDATE, I GET DISTRACTE....OH LOOK AT THAT... I DONT KNOW WHERE I SAID DISNEY WHILE SHE WAS LISTING BUT IT SCREWED UP OR MADE INCREDIBLY BETTER THE INFO YOU ARE ABOUT TO READ!!!!!!! IM PRETTY SURE I SAID DIXIE VIBE...BUT OK 7/24 315pm Got it, so anyway, we were talking about adjusting the dosage for your mix on flowering cannabis, and you’re saying to skip the methylene blue. Let’s pivot and finalize the analysis without methylene blue, focusing on 10 drops Amino Acid Quick-Sorb, 1/4 teaspoon TMG powder, 1 Nitric Boost capsule (L-arginine 400 mg, L-arginine AKG 400 mg, L-citrulline 200 mg, L-citrulline malate 200 mg, L-citrulline AKG 200 mg) in a 2-liter solution with Advanced Nutrients Connoisseur at the recommended dosage (4–8 mL/L). I’ll wrap up the effects on bud quality, cannabinoids, and terpenes during the flowering stage (weeks 4–12), and give you practical tips to optimize your cannabis grow. ### Effects During Flowering Stage (Without Methylene Blue) 1. Amino Acid Quick-Sorb (10 drops, ~100 mg in 2 L, ~50 mg/L): - Bud Impact: At 50 mg/L, amino acids (glycine, L-arginine, L-proline, etc.) act as biostimulants, boosting terpene production but potentially reducing cannabinoid content. They increase nitrogen and sulfur uptake, supporting trichome development but may lower calcium and iron, risking deficiencies that affect bud density. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/) - Cannabinoids: Reduces CBDA by up to 44% and THCA by up to 196% (especially in drain-to-waste systems), lowering potency. Recirculation systems mitigate this loss slightly. (https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.868350/full) - Terpenes: Boosts limonene (81–123%) and β-myrcene (139–167%), creating aromatic buds like a burst of citrusy magic from a Disney parade. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/) - Flowering Note: The low dose minimizes nitrogen overload, but excess could delay bud ripening, making buds leafy like overgrowth in Sleeping Beauty’s castle. Monitor for yellowing leaves (calcium/iron deficiency). 2. Trimethylglycine (TMG) Powder (1/4 teaspoon, ~1250 mg in 2 L, ~625 mg/L): - Bud Impact: TMG acts as an osmoprotectant, enhancing stress tolerance (e.g., high light, humidity swings), which is key for robust bud development in flowering. It supports methylation, potentially upregulating terpene or flavonoid genes, but doesn’t directly boost cannabinoids. (https://en.wikipedia.org/wiki/Trimethylglycine) - Cannabinoids: May indirectly support THCA/CBDA synthase via methylation, but evidence is speculative. No significant direct impact. - Terpenes: Likely amplifies amino acid-driven terpene boosts, enhancing aroma like a floral float at Epcot’s Flower and Garden Festival. - Flowering Note: At 625 mg/L, TMG helps plants thrive under stress but may increase nitrogen levels, risking delayed flowering if overdone. Watch EC to avoid burn. 3. Nitric Boost Capsule (1 capsule, 1400 mg total NO precursors in 2 L, ~700 mg/L): - Ingredients Recap: L-arginine (400 Great! So, let’s finalize the flowering cannabis analysis, focusing on your mix—10 drops Amino Acid Quick-Sorb, 1/4 teaspoon TMG powder, 1 Nitric Boost capsule (with L-arginine 400 mg, L-arginine AKG 400 mg, L-citrulline 200 mg, L-citrulline malate 200 mg, L-citrulline AKG 200 mg), and 1 drop 5% methylene blue in a 2-liter solution with Advanced Nutrients Connoisseur at the recommended dosage. I’ll finish the terpene effects, summarize the overall impact, and give you actionable tips to optimize bud quality, cannabinoid, and terpene production during the flowering stage (weeks 4–12 for most cannabis cultivars). Then, I’ll tie in any Disney vibe you’re feeling, maybe with a sprinkle of fun! — ### **Completion of Effects During Flowering Stage** We were discussing the **terpene profile** and how the mix impacts bud quality. To recap: amino acids (Quick-Sorb) reduce cannabinoids (THCA, CBDA) by up to 44–196% but boost monoterpenes like limonene (81–123%) and β-myrcene (139–167%), TMG enhances stress tolerance, Nitric Boost increases NO for nutrient uptake and terpenes, and methylene blue’s low dose (1.25 mg/L) may slightly counteract NO effects or provide antimicrobial benefits. Let’s finish the terpene section and synthesize the rest. - **Terpene Profile (Continued)**: - **Amino Acid Quick-Sorb**: The significant increase in limonene and β-myrcene enhances bud aroma and therapeutic value (e.g., anti-inflammatory, sedative effects). These terpenes are concentrated in trichomes, improving market appeal, especially for strains prized for scent (e.g., OG Kush, Blue Dream). Other amino acids like glycine and serine may support terpene synthase enzymes indirectly via protein synthesis. - **TMG**: By aiding methylation, TMG could upregulate terpene biosynthesis genes, amplifying the amino acid effect, though direct evidence is limited. - **Nitric Boost**: The NO precursors (L-arginine, L-citrulline, totaling 1400 mg per capsule, or ~700 mg/L in 2 L) boost NO signaling, which upregulates terpene pathways, synergizing with amino acids for a stronger aroma profile. - **Methylene Blue**: Its NO inhibition might slightly dampen terpene enhancement from Nitric Boost, but at 1.25 mg/L, the effect is negligible. Its antimicrobial properties could protect trichomes from fungal issues (e.g., bud rot), preserving terpene quality. - **Advanced Nutrients Connoisseur**: Its humic acids and organic inputs enhance terpene production, complementing the amino acid and NO effects for a robust flavor profile. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/, https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.868350/full, https://www.advancednutrients.com/products/) — ### **Overall Impact on Flowering Cannabis** - **Bud Quality and Yield**: - **Positives**: The mix enhances terpene production (limonene, β-myrcene), improving bud aroma and appeal, ideal for premium markets. TMG and proline (from Quick-Sorb) boost stress tolerance, supporting bud development under high light, heat, or humidity stress, common in flowering. Connoisseur’s optimized NPK (e.g., ~194 mg/L N, 59 mg/L P) ensures dense, resinous buds. Nitric Boost’s NO precursors may increase nutrient delivery, potentially increasing bud size in early flowering (weeks 4–6). - **Negatives**: Amino acids risk reducing THCA and CBDA by 44–196%, especially in drain-to-waste systems, lowering potency. Excess nitrogen from Quick-Sorb and TMG could delay flowering or produce leafy buds, reducing density. Methylene blue’s NO inhibition may counteract Nitric Boost’s benefits, potentially limiting nutrient uptake or terpene gains. High EC (estimated 1.8–2.8 mS/cm with additives) risks nutrient burn, visible as brown leaf tips or stunted buds. 7/25 9am Water today no MB, all aminos tho. 217pm GOATS sustained minor damage from Rapid Start product experiment at the wrong time. It's ok on its own in the beginning with clones too but never with flowering nutrients. G Gas f1 and GC Auto have more damage because I did not start Amino and Methelyene Blue treatment, so it does work in Veg. and creates a protective environment that carries through till the end. No more MB or Aminos, I WILL keep using small amounts of TMG Powder. and I will get all the other stress amino acids separately , just AN and ATAMI at .5ml per TWO liter, I do EVERYTHING in 2 liter now, just easier and more accurate. NEVER FUCKING SLEEP...FUCK 745PM.... AMINO'S HAVE BETRAYED ME!!!!!! FARGING BASTAGES!!!! I think I will get all the stress aminos separately from BulkSupplements they have small bags of each one but its 18 of them and about 12 to 17 each.....BulkSupplements. 137am. Something smoking Purple Haze...puts me to sleep....nooooo. ZUP by Fastbuds smell like. garlic cheese muffin its just greatl. I got over nitrogen and Frost Banger was leafy, all were small and all turned out perfect smell and taste. 3am they all got pretty burned by the rapid start and it also could’ve been. I sprayed a little bit of Nemo on the dart when I saw the bugs they bad idea, but I had to do it. 7/27 1233am. IVE SEEN RE-VEGES YOU PEOPLE WOULDN'T BELIVE ... UNTIL NOW LOSERS.. the Tyson plants are re-vegging perfectly!!!! in a couple months they will be strong and small. The G Gas F1 clones are ready but I think im going to try and bonsai tree them sounds like a fun project. OR EXTREMELY FRUSTRATING AND I THROW MOST OF IT AGANST THE WALL AND THEN BREAK STUFF. NOT SURE ITS WORTH IT. 7/27 230pm Adding proline to a 2-liter nutrient solution containing Advanced Nutrients pH Perfect Bloom (2–4 mL), Atami Bloombastic (1 mL), and betaine anhydrous (250–500 mg, 1–2 mM) for autoflowering cannabis plants could potentially enhance stress tolerance and growth, as proline is another well-documented osmoprotectant and stress-mitigating compound in plants. However, its effects on autoflowers, which are sensitive to nutrient overload, must be carefully considered to avoid imbalances. Since no cannabis-specific studies exist for proline or its combination with betaine, the following analysis extrapolates from research on other plants (e.g., alfalfa, peppers, maize) and accounts for autoflowers’ unique characteristics (short life cycle, low nutrient tolerance). Background on Proline • What is Proline?: Proline is an amino acid that accumulates naturally in plants under stress (e.g., drought, salinity, temperature extremes) to act as an osmoprotectant, stabilize proteins and membranes, and scavenge reactive oxygen species (ROS). It complements betaine’s role as an osmolyte and stress mitigator. • Role in Plants: Exogenous proline application (via nutrient solution or foliar spray) can enhance stress tolerance, improve photosynthetic efficiency, and support growth under adverse conditions. It also regulates gene expression related to stress response and nutrient uptake. • Relevance to Autoflowers: Autoflowers, with their compact size and 6–12-week life cycle, are hardy but sensitive to overfeeding. Proline could enhance resilience in stressful environments (e.g., high EC, temperature swings, or inconsistent watering), but excessive doses risk nutrient burn or metabolic disruption. Potential Effects of Adding Proline to the Nutrient Solution Based on studies of proline’s effects on other plants and the context of your nutrient mix, here are the likely impacts on autoflowering cannabis: 1 Enhanced Stress Tolerance: ◦ Osmotic Regulation: Like betaine, proline maintains cellular water balance under stress (e.g., drought, salinity). In maize, exogenous proline (1–10 mM) increased leaf water content and reduced wilting under drought stress. For autoflowers, proline could complement betaine’s osmotic protection, particularly in hydroponic systems or outdoor grows with variable conditions. ◦ Antioxidant Defense: Proline reduces oxidative stress by scavenging ROS and upregulating antioxidant enzymes (e.g., superoxide dismutase [SOD], peroxidase [POD]). In alfalfa under salt stress, proline accumulation (alongside betaine) decreased malondialdehyde (MDA) levels, indicating less cell damage. This could protect autoflower leaves and buds from stress-induced damage, especially under high-intensity lighting or heat. 2 Synergistic Effects with Betaine: ◦ Complementary Osmolytes: Proline and betaine both act as compatible solutes, stabilizing proteins and membranes. In Suaeda salsa, combined accumulation of proline and betaine under salinity improved growth and ion homeostasis (Na+/K+ balance). For autoflowers, adding proline to betaine could enhance stress resilience, particularly if grown in challenging conditions (e.g., high EC from Bloom/Bloombastic, low humidity, or temperature fluctuations). ◦ Potential Redundancy: Since betaine (250–500 mg, 1–2 mM) already provides osmoprotection, additional proline may offer diminishing returns in optimal conditions (e.g., controlled indoor grows with 18–20 hours light, 70–80°F, 40–60% humidity). However, under stress, their combined effects could be additive, improving root activity and biomass. 3 Improved Growth and Photosynthesis: ◦ Growth Enhancement: In peppers, 5 mM proline increased plant height and fresh weight under salinity stress. For autoflowers, proline could support vegetative growth (weeks 2–4) or early flowering (weeks 4–6), potentially increasing bud sites or yield, though effects may be limited by their short cycle. ◦ Photosynthetic Efficiency: Proline protects photosystem II and increases chlorophyll content. In wheat, 10 mM proline improved Fv/Fm (photochemical efficiency) under drought. For autoflowers under high-intensity lights (e.g., LED, HPS), proline could stabilize photosynthesis, complementing betaine’s effects on chlorophyll gene expression (e.g., Capsb genes in peppers). 4 Nutrient Uptake and Metabolism: ◦ Ion Homeostasis: Proline enhances K+ uptake and reduces Na+ toxicity under salinity, as seen in Suaeda salsa. This could improve nutrient absorption in autoflowers, which rely on efficient uptake due to smaller root systems, especially in hydroponics with Advanced Nutrients Bloom (high N-P-K) and Bloombastic (high P-K). ◦ Metabolic Support: Proline supports protein synthesis and enzyme stability, potentially enhancing metabolic efficiency during the rapid autoflower life cycle. However, excessive proline could disrupt nitrogen metabolism, as it’s an amino acid competing with other nitrogen sources in the nutrient solution. 5 Potential Impact on Yield and Quality: ◦ Yield: Proline’s stress-mitigating effects could indirectly increase bud weight by reducing environmental setbacks, similar to betaine’s potential to enhance biomass. Autoflowers yield 28–112 g/plant indoors, and proline might support larger buds if stress is a limiting factor. ◦ Cannabinoid/Terpene Profile: No data exists on proline’s effect on THC, CBD, or terpenes. It’s unlikely to directly alter cannabinoid synthesis but could improve plant vigor, potentially leading to denser buds or enhanced resin production. Overapplication risks altering flavor if not flushed properly. Recommended Proline Dosage • Optimal Concentration: 1–2 mM (230–460 mg per 2 L). ◦ Calculation: Proline molecular weight: 115.13 g/mol. 1 mM = 0.001 mol/L × 115.13 g/mol = 0.11513 g/L = 115.13 mg/L. For 2 L at 1 mM: 115.13 mg/L × 2 L = 230.26 mg (rounded to 230 mg). For 2 L at 2 mM: 115.13 mg/L × 2 × 2 L = 460.52 mg (rounded to 460 mg). ◦ Rationale: Studies (e.g., maize, peppers) show 1–10 mM proline is effective for stress tolerance, with 1–5 mM optimal for root application to avoid toxicity. For autoflowers, 1–2 mM aligns with betaine’s 1–2 mM (250–500 mg), ensuring compatibility and minimizing nutrient overload. Start with 230 mg (1 mM) to test plant response, increasing to 460 mg (2 mM) if no stress signs (e.g., leaf burn, stunted growth) appear after 1–2 applications. • Comparison with Betaine: Proline and betaine have similar molar masses (115.13 vs. 117.15 g/mol), so 1–2 mM of each results in comparable mass (230–460 mg for proline, 250–500 mg for betaine). Their combined effect should stay within autoflower EC limits (1.2–1.8 mS/cm for flowering). Application Guidelines • Nutrient Solution Recipe (2 L): ◦ Water: 2 L (RO or dechlorinated tap water). ◦ Advanced Nutrients pH Perfect Bloom: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). ◦ Atami Bloombastic: 1 mL (0.5 mL/L, suitable for flowering autoflowers). ◦ Betaine Anhydrous: 250–500 mg (1–2 mM, start with 250 mg). ◦ Proline: 230–460 mg (1–2 mM, start with 230 mg). ◦ Mixing: Add Bloom first, then Bloombastic, betaine, and proline, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). • Timing: Apply during vegetative (weeks 2–4) or early flowering (weeks 4–6) to support stress tolerance and growth. Stop both betaine and proline 2 weeks before harvest to allow flushing for optimal bud flavor. • Frequency: Use with each nutrient feeding (e.g., every 2–3 days in hydro, weekly in soil). Alternate or reduce frequency if EC approaches 1.8 mS/cm. • Source: Use L-proline (plant-grade, 99%+ purity) from a reputable supplier (e.g., agricultural or lab-grade sources). Avoid impure or non-plant-specific products. Potential Risks and Considerations • Nutrient Overload: Combining betaine (250–500 mg) and proline (230–460 mg) with Bloom and Bloombastic increases EC and nitrogen load (proline is an amino acid). Monitor for nutrient burn (brown leaf tips, clawing). If symptoms appear, reduce both to 0.5 mM (125 mg betaine, 115 mg proline per 2 L) or skip a feeding. • EC Impact: Betaine and proline each add ~0.1–0.2 mS/cm at 1–2 mM. With Bloom (1–2 mL/L) and Bloombastic (0.5 mL/L) at ~1.0–1.5 mS/cm, the total EC should stay below 1.8 mS/cm. Measure after mixing. • Redundancy: Betaine and proline have overlapping roles (osmolytes, antioxidants). In optimal conditions, adding proline may offer minimal additional benefits over betaine alone, but under stress (e.g., high heat, low humidity), their synergy could enhance resilience. • Cost vs. Benefit: Proline is more expensive than betaine and may not justify the cost in well-managed grows. Test its impact on a small scale first. • Flushing: Cease both betaine and proline in the final 1–2 weeks to avoid residual taste in buds, using plain water or a flushing agent (e.g., Advanced Nutrients Flawless Finish). • Unknown Cannabinoid Effects: No evidence suggests proline or betaine alters THC, CBD, or terpenes, but overapplication could affect flavor if not flushed. Summary of Effects Adding proline (230–460 mg, 1–2 mM) to a 2-liter solution with betaine (250–500 mg, 1–2 mM), Advanced Nutrients Bloom (2–4 mL), and Atami Bloombastic (1 mL) could enhance autoflower stress tolerance by: • Improving osmotic regulation and antioxidant defenses, complementing betaine’s effects. • Supporting photosynthesis and growth under stress (e.g., salinity, heat, low light), potentially increasing biomass or bud yield. • Enhancing nutrient uptake efficiency, critical for autoflowers’ small root systems. Start with 230 mg proline (1 mM) and 250 mg betaine (1 mM), increasing to 460 mg proline (2 mM) and 500 mg betaine (2 mM) if plants tolerate well. Apply during vegetative or early flowering, monitor EC (1.2–1.8 mS/cm), and stop 2 weeks before harvest. Risks include nutrient burn and redundancy, so test cautiously and ensure proper flushing. If you provide your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine the dosing or application method. Let me know if you need sourcing tips for proline or further guidance!”” 7/27 3PM. Adding **0.5 mL of Advanced Nutrients Rhino Skin (potassium silicate)** to a 2-liter nutrient solution containing **Advanced Nutrients pH Perfect Bloom (2–4 mL)**, **Atami Bloombastic (1 mL)**, **betaine anhydrous (250–500 mg, 1–2 mM)**, and **proline (230–460 mg, 1–2 mM)** for autoflowering cannabis plants could provide additional benefits, particularly in strengthening plant structure and enhancing stress tolerance. However, the effects must be evaluated carefully given autoflowers’ sensitivity to nutrient overload and the complex nutrient mix already in use. Below, I analyze the potential effects of adding 0.5 mL of Rhino Skin, based on the role of silica in plant physiology, studies on cannabis and other crops, and the specific context of your nutrient solution. ### **Background on Silica (Advanced Nutrients Rhino Skin)** - **What is Rhino Skin?**: Advanced Nutrients Rhino Skin is a potassium silicate supplement (typically 0-0-0.4 N-P-K, with ~4–7% silica as SiO₂). Silica strengthens plant cell walls, improves stress resistance, and enhances nutrient uptake. The recommended dosage is 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L) due to their sensitivity. - **Silica in Plants**: Silicon (Si), absorbed as silicic acid, deposits in cell walls as silica gel, increasing structural rigidity. It also mitigates abiotic (e.g., drought, heat) and biotic (e.g., pests, fungi) stresses and can improve photosynthesis and yield. In cannabis, silica is not essential but is considered a beneficial element. - **Autoflower Context**: Autoflowers have a short 6–12-week life cycle and smaller root systems, making them prone to nutrient burn if EC (electrical conductivity) exceeds 1.2–1.8 mS/cm during flowering. Rhino Skin’s low dosage (0.5 mL per 2 L, or 0.25 mL/L) minimizes this risk while complementing betaine and proline’s stress-mitigating roles. ### **Potential Effects of Adding 0.5 mL Rhino Skin** Based on silica’s effects in cannabis and other plants (e.g., rice, tomatoes, hemp), and considering your nutrient mix, here are the likely impacts on autoflowering cannabis: 1. **Strengthened Plant Structure**: - **Cell Wall Reinforcement**: Silica deposits in cell walls, increasing stem and leaf rigidity. In hemp (*Cannabis sativa*), silica supplementation (e.g., 1–2 mM Si) thickened stems and improved mechanical strength. For autoflowers, which are compact (1–3 feet tall), 0.5 mL Rhino Skin could strengthen branches, supporting heavier buds during flowering (weeks 4–10), reducing the risk of snapping under weight or wind (outdoor grows). - **Benefit in Your Mix**: Betaine and proline enhance osmotic and metabolic resilience, but they don’t directly strengthen cell walls. Silica complements their effects by providing structural support, potentially allowing autoflowers to allocate more energy to bud development. 2. **Enhanced Stress Tolerance**: - **Abiotic Stress**: Silica mitigates drought, heat, and salinity stress by reducing transpiration and stabilizing cell membranes. In tomatoes, 1 mM silica improved water retention under drought. For autoflowers, 0.5 mL Rhino Skin (~0.1–0.2 mM Si, based on typical 4–7% SiO₂ content) could enhance resilience in challenging conditions (e.g., low humidity, high temperatures, or high EC from Bloom/Bloombastic). - **Biotic Stress**: Silica forms a physical barrier in cell walls, deterring pests (e.g., spider mites) and pathogens (e.g., powdery mildew). In cannabis, silica reduced fungal infections in controlled studies. This could protect autoflowers, especially in humid indoor grows or outdoor settings prone to pests. - **Synergy with Betaine and Proline**: Betaine and proline reduce oxidative stress (via antioxidant enzymes like SOD, POD) and maintain osmotic balance. Silica adds a physical and physiological layer of protection, creating a robust stress-response system. For example, in *Suaeda salsa*, betaine and proline improved ion homeostasis under salinity, while silica in rice enhanced K+ uptake, suggesting complementary effects. 3. **Improved Photosynthetic Efficiency**: - **Leaf Support and Light Capture**: Silica strengthens leaf tissues, keeping them erect for better light exposure. In rice, 1.5 mM silica increased chlorophyll content and photosynthetic rates. For autoflowers under high-intensity lights (18–24 hours), 0.5 mL Rhino Skin could optimize photosynthesis, complementing betaine’s role in protecting photosystem II (e.g., upregulation of Capsb genes in peppers). - **Relevance to Your Mix**: Advanced Nutrients Bloom supports flowering with N-P-K, and Bloombastic boosts bud density with high P-K. Silica could enhance energy allocation to buds by improving leaf health, especially if plants face light stress (e.g., inconsistent outdoor light). 4. **Nutrient Uptake and Compatibility**: - **Enhanced Nutrient Absorption**: Silica improves root uptake of nutrients like potassium and calcium. In maize, 1 mM silica increased K+ and Ca²⁺ levels. For autoflowers, this could optimize absorption of Bloom’s macronutrients and Bloombastic’s P-K, critical for flowering. - **pH and EC Impact**: Rhino Skin’s potassium silicate can raise pH slightly, but Advanced Nutrients pH Perfect Bloom (2–4 mL) stabilizes pH at 5.5–6.5 (hydro) or 6.0–6.5 (soil). At 0.5 mL per 2 L (0.25 mL/L), Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), keeping the total EC (Bloom + Bloombastic + betaine + proline + silica) within 1.2–1.8 mS/cm for flowering autoflowers. - **Compatibility with Betaine and Proline**: Betaine (1–2 mM) and proline (1–2 mM) are neutral organic compounds that don’t chemically interact with silica (an inorganic compound). Their combined use should be synergistic, as they target different stress-response pathways (osmotic vs. structural). 5. **Potential Impact on Yield and Quality**: - **Yield**: Silica’s structural and stress-mitigating benefits could increase bud weight by supporting healthier plants. In hemp, 1 mM silica increased biomass by 10–15%. For autoflowers (28–112 g/plant indoors), 0.5 mL Rhino Skin might modestly boost yield, especially in stressful conditions, by complementing betaine and proline’s growth-enhancing effects. - **Cannabinoid/Terpene Profile**: No direct evidence links silica to THC, CBD, or terpene changes in cannabis, but stronger plants may produce denser buds or more resin. Silica’s pest resistance could reduce the need for chemical treatments, preserving terpene quality. Proper flushing remains critical to avoid flavor impacts. ### **Recommended Dosage and Application** - **Dosage**: **0.5 mL Rhino Skin per 2 L (0.25 mL/L)** is optimal. - **Rationale**: Advanced Nutrients recommends 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L). At 0.5 mL per 2 L, Rhino Skin provides ~0.1–0.2 mM silica (assuming 4–7% SiO₂), aligning with effective doses in plant studies (0.5–2 mM) while minimizing EC and pH impacts. - **Confirmation**: Your specified 0.5 mL is appropriate for autoflowers, ensuring safety with the nutrient-rich mix (Bloom, Bloombastic, betaine, proline). - **Nutrient Solution Recipe (2 L)**: - **Water**: 2 L (RO or dechlorinated tap water). - **Advanced Nutrients pH Perfect Bloom**: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). - **Atami Bloombastic**: 1 mL (0.5 mL/L, suitable for flowering). - **Betaine Anhydrous**: 250–500 mg (1–2 mM, start with 250 mg). - **Proline**: 230–460 mg (1–2 mM, start with 230 mg). - **Advanced Nutrients Rhino Skin**: 0.5 mL (0.25 mL/L). - **Mixing**: Add Bloom first, then Bloombastic, betaine, proline, and finally Rhino Skin, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Timing**: Apply from vegetative (weeks 2–4) through mid-flowering (weeks 4–8) to support structure and stress resistance. Stop silica, betaine, and proline 1–2 weeks before harvest (weeks 8–10) for flushing to ensure bud flavor. - **Frequency**: Use with each nutrient feeding (every 2–3 days in hydro, weekly in soil). If EC nears 1.8 mS/cm, reduce Bloom to 2 mL or alternate silica applications. ### **Potential Risks and Considerations** - **Nutrient Overload**: The mix (Bloom + Bloombastic + betaine + proline + silica) is nutrient-dense. At 0.5 mL, Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), but the total EC (1.2–1.8 mS/cm) must be monitored to avoid burn (brown leaf tips, clawing). If symptoms appear, reduce Bloom to 2 mL or skip silica for one feeding. - **pH Stability**: Rhino Skin’s potassium silicate may slightly raise pH, but pH Perfect Bloom should counteract this. Verify pH after mixing (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Redundancy**: Betaine, proline, and silica all enhance stress tolerance (osmotic, metabolic, structural). In optimal conditions (e.g., indoor, 70–80°F, 40–60% humidity), silica’s benefits may be marginal, but under stress (e.g., heat, pests), it adds a complementary layer of protection. - **Flushing**: Stop all additives (silica, betaine, proline) 1–2 weeks before harvest, using plain water or Advanced Nutrients Flawless Finish to avoid residual taste in buds. - **Cost vs. Benefit**: Rhino Skin is relatively affordable, but its benefits may be less pronounced in well-managed grows. Test on a few plants to assess impact. - **Cannabinoid Effects**: No evidence suggests silica alters THC, CBD, or terpenes, but improved plant health could enhance resin production. Overuse risks silica deposits affecting flavor if not flushed. ### **Summary of Effects** Adding **0.5 mL Advanced Nutrients Rhino Skin** to a 2-liter solution with **Bloom (2–4 mL)**, **Bloombastic (1 mL)**, **betaine (250–500 mg)**, and **proline (230–460 mg)** could: - Strengthen stems and leaves, supporting heavier buds. - Enhance resistance to abiotic (drought, heat) and biotic (pests, fungi) stresses. - Improve photosynthetic efficiency and nutrient uptake, complementing betaine and proline’s osmotic and metabolic benefits. - Potentially increase yield (28–112 g/plant) by reducing stress setbacks. The 0.5 mL dose (0.25 mL/L) is safe for autoflowers, keeping EC within 1.2–1.8 mS/cm. Apply during vegetative to mid-flowering, stop 1–2 weeks before harvest, and monitor for nutrient burn. The synergy of silica (structural), betaine (osmotic), and proline (metabolic) could optimize autoflower health, especially under stress. If you share your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine dosing or application further. Let me know if you need sourcing tips or additional guidance!

7 week is behind us. On day 45 i setting up net for holding buds and a bit more fullit up tent. There is past 2 days since she started flower. In this update i can not move out of grow room and some picture is not so perfect Peace and love ✌️😁💜💚

Well, that day has come, the girl would have been really different if not for the high temperature, strong light, and a huge lack of potassium :( well, everything will be fine next time :) live and learn :) I am satisfied with the result, which is what I need :) got 200 gr of liquid :) gathered a lot of sugar leaf :) they are already in the sweet shop :D I will make an addition!! good growth and success :)

Sin problemas. Siguen muy fuertes, ya no tengo espacio en altura, llegan a 5cms del foco. Esta semana introduzco un booster orgánico y un potenciador de flora de aptus. Todo orgánico. Siguen engordando sin problemas, una presenta algunas hojas amarillas, parece insuficiencia de nitrógeno, pero lo veo normal en el momento que estan.

Day 29... afternoon very sunny and warm temp 👍 Day 30 damn hot and full of sun 😎👍 Day31 warm weather with sun in the morning, laters cloudy with high humidity 😃 Day32 totally fucked up weather...rain non stop and cloudy 😕😫😩 Day33 same damn weather then yesterday oh lord 😒😠😵 Day34 cloudy start in the day but then sunny weather 😍👍😏 Day35 normal day with sun and clouds not too hot 😎👌 Enjoy week 5 speed run vid 😉👍

Starring to put on some weight. Dealing with a calcium problem most likely due to me only learning this week I should be buffering my coco with a calmag bath for 8+ hours atleast twice lol live and learn.

Day 28: nutrients like plan --------------------------------------------- Not much to say, we are in bloom now. Happy growing 🍧✌️

Week 2 : Colorado Cookies in Biotabs Biotabs are drying much quicker than the other pots because they're closer to the fan, so i'll tune the air control in by having the fan pointing upwards instead. They got watered with micro from my very own specialized micro , one tablespoon per L, aloe gel from my kitchen plant, and (regretfully) a bit of lemon juice to pH it. didnt realise the organic would be no match for the aloe pH wise, so i'm spraying 1ml of my very own amino concentrate directly on the soil , not on the plant. i do one spray in the morning one spray at night, for 10 days to try to get the pH normalised to 6 , the night time is just enough to mix one spray can of 250 ml with 1/4 ml of amino concentrate, and have some left over. i added maybe 160 ppm of aloe to the watering. I sprayed the leaves once or twice max with 1ml/L spray and 120 cal mag on day 5. VPD went from week one 0.6 to 0.8 to pretty stable at 1.0. got my laser thermometer in the mail, so that really helps ! very even phenos across the colorado cookies so far, three nodes, very straight, very hungry. they seem to be enjoying having the roots a bit too cold more than the others. in soil ppm went from 340 in week one to 420 in week 2 🚀

One more week with no more than just appreciating these babies growing. After one feed with just water this week I feed them with Biobizz nuts once more. Following the same chart as last week.

Just got a new 600w mh/hps light for now it’s in my current setup with my 250 n it’s dimmed to 50%, only reason I mention is b/c the plants seem to be loving it!! They’re 12 days old from seed, still just ph’d water for them! So far so good :P

We Are Very Happy To Be Present At The Spannabis

RQS Titan F1 week 5 Hello friends and followers, 2/25 This has been an exciting week. My Titan girls started flower 🌼. T3 in the 3-gallon air pot is larger at 6.5 inches. Last week I noticed her new growth was getting long and thin. A sure sign the pistils will emerge in a day or two. T3 started flower 🌼on 2/25. 2/26 Surprise surprise, T3 stretched an inch overnight and T2 about a.5 inch. That stretch meant I had a busy plant workday ahead. First, I defoliated T3 since I could see what I had to work with. Then because I’ve been following @yan402 on his Titan grow, I said I was going to top her. He said its ok if done correctly and at the right time. Well, fingers crossed 🤞, I hope it was not too late or cut in yhe wrong place since T3 was already in flower. Her short (.75-1” long) stems are so close to the stem, it was difficult getting the tie down pins placed. With T2’s stretches her tippy top is just peeking above the copper pot rim. She is towering 😅 at 2.5 inches. T2 was defoliated on 2/26 as well. Her defoliation pictures above show how many I was able to trim off 7 and the leaf relative size. T2’s largest leaf is the size of a quarter and the smallest set I cut off, about the size of a penny. I keep supplementing and folar spraying with Cal-mag but the internodal chlorosis is still visible. I think Teapot gal (T2) has a copper excess.  Slowed Growth,  Fewer branches  Internodal Chlorosis  Stumpy roots (I won’t see this until I harvest and reveal the root ball) Given these symptoms of copper excess, and that RQS Titan are F1 hybrids and should be stable, I believe the height and the health differences are attributable to the copper wire teapot. They are in the same soil, same tent environment, same nutrient solution, same light, same everything. As I stated in previous discourse, I expected the overall plant to be smaller than T3 simply based on the pot size but did not expect to see such size differences so early, not to mention the nutrient issues. How I wonder? The copper wire is separated from the plant and soil by coco liner (which should be neutral, then coffee filters because I thought the soil would was out). Then the soil and plant. T2 is towering at 2.5 inches and started flower on 2/29. I expected a delay in her flowering time (4 days after T3) since she was transplanted to the teapot when T1 failed to thrive (maybe also the teapot cause?). Any time you train your plants, the stress causes delays in flowering then harvest. I defoliated T2 on 3/1 day 30. The pictures after defoliation show the tight node structure with 3 or 4 nodes crammed in only 1.5 inches of the main stem. Sheesh. So, as we enter March, we are off and growing buds. RQS says harvest 70-75 days or 40-45 days of flower. My calculations – harvest will be April 13-18. Be sure to come for my tea

I like how it looks like ... What u mean folks?

Watered with 1 gallon of the above listed nutrients - dry in 3 days, ready for another dosage! Very Frosty! Very Smelly! Kinda-Happy-Kinda-Grumpy! :D She's just fine!