2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Nugs gettin fat

23.11.25 So our last week started, she is looking nice and ready and she is gaining weight. I love the pink hints on it she looks very beautiful 😍 ❤️ Can't wait to try it out. Hopefully she is as delicious as she looks 💦 24.11.25 So today I made some small amounts of char by rubbing her a bit as the tircomes are still clear so I decided to add some extra food and maybe stress here a bit. To make it grow more tircomes hopefully 😅 And Manged to up the humidity by closing the windows in the room, not much and I have to run the fan at the lowest! German growers how it's for you ? I feel the winter is abit dry. 25.11.25 The char was out of this world 🌎 tasted so delicious 🤤 . The smell is getting stronger. I can finally see some red tircomes in the lower buds And hopefully hopefully she can fully recover them and get more fatty and sticky. And for today feed I just used only water. 26.11.25 So finally getting humidity under control, I started misting with water which helped with getting the humidity up. And for feeding pH adjusted water. Tircomes seems to be recovering. I would not recommend making char at this stage of flowering maybe early stage or mid will be better, as it really damage the tircomes heads.

Idhdb

growing rapidly, next week I plan to add fertilizers, at the current stage the plant has gone through LST and topping and pruning large fan leaves, in my opinion this stress did not affect the growth this is my first experience in the style of Maine Lining, waiting for 4 week!

Sky Walker ~~~~~INDOOR~~~~~ DAY 148 Above Dirt 💬SATURDAY 01.20.24 - The girls are starting to hum again after last week's harsh defoliation, today will be using Real Growers Recharge for their weekly Tea Feeding, will be top dressing again next week, and this may carry the girls until the end of thier grow cycle. 💬 MONDAY 01.22.24 - 6 solid weeks into flower and these ladies are starting to fatten up their buds, they are already becoming hard as rocks, I don't think they liked the heavy defoliation I put them through and may have to be content with smaller flowers, or, maybe not, we will see. The smells are amazing with a dank and earthy fragrance also some prominent citrus and fruity notes. 📝 REAL GROWERS RECHARGE -is a mix-and-pour soil microbe superpack. It works like an instant compost tea. The soil microbes in Recharge go out and grab the nutrients in your soil, help break them down, and bring them back to your plant's root zone. ~~~~~~~~~~~~~~~ 🌱Sky Walker 👨‍🌾Sativa Jones 🌤️@Medicgrow420 📝SeedBank Seeds 📝@gaiagreenorganics 📝Bokashi Biochar 📝Real Growers Recharge 📝 ProMix-HP ~~~~~~~~~~~~~~~ 📝 Skywalker OG Strain is a top-shelf Indica dominant hybrid. It is a cross of Original Amsterdam Blueberry, OG Kush, and the indica strain Mazar from Afghanistan, and is best known for its super potent effects and sky-high THC levels.

MrJones Girl Scout Cookies ╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰WEEKLY GOALS╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰ 🌞Environment - 75/80℉ and 55% Humidity 💧 Feeding - Using "Super Coco" amended soil. compost teas, Dechlorinated H20 PH/6.5 Fish Fertilizer, about 4 ounces of water per day. 🍃Training / Finished the LST yesterday, keeping side branches pulled down, also working on the single plant that was topped to produce 8 colas. 🕷️ IPM - Will be using Green Cleaner" 1 OZ per Gallon, and CannControl from Mammoth alternating between product each month for Integrated Pest Management. 💡240W QB288 V3 LM301H 4000k 660nm Red ╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰🍪╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰🍪╰⊰🍪╰⊰🍪⊱╮ Rambling - Moving onto week 4 these ladies are doing great, just growing vertically nicely, they are about to flip into flower. ╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰🍪╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰🍪╰⊰🍪╰⊰🍪⊱╮ 🔶Saturday 12.12.20 / Watered w/ Dechlorinated H20 PH to 6.2/6.5 🔶Sunday 12.13.20 / Watered w/ Dechlorinated H20 PH to 6.2/6.5 🔶Monday 12.14.20 / Watered w/ Dechlorinated H20 PH to 6.2/6.5 🔶Tuesday 12.15.20 / Original Sensible Seeds Girl Scout Cookies is rocking it, they are growing like crazy, the One plant that I decided to mini mainline is doing well for all the pruning and training done in a short period of time, she is vegging back to life nicely, her H20 consumption is down, could be lack of foliation, or maybe stunted, the next few days will tell a lot. 🔶Wednesday 12.16.20 / Watered w/ Dechlorinated H20 PH to 6.2/6.5 🔶Thursday 12.17.20 / Adjusted the tie downs today, did light defoliation, pots were pretty full so no water today - tomorrow will be feeding with Recharge. The plant that I topped is looking great and growing out, will have 8 amazing cola's!! 🔶Friday 12.18.20 / No watering today. ╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰🍪╰⊰🍪╰⊰´🍪⊱╮🍪╰⊰🍪╰⊰🍪╰⊰🍪⊱╮ SOIL MIX COCO - 70/30% Tupur Royal Gold 30 GALLON WORM CASTINGS 5 GALLON LOBSTER COMPOST 5 GALLON PERLITE 6 GALLON INSECT FRASS 2 CUP ORGANICALLY DONE GROW 5 CUPS BUILD A SOIL COCO MINERAL MIX 6 CUPS

She is growing bigger and bigger buds are everywhere, did not expect that size and my room size is on his limits with this plant, she would be perfect for heavy LST. Once she will be ready for harvest she will be a beast covered in fat juicy bud shining like diamonds.

The strain is not the biggest yielder but its quite promissimg,the smell is top and the effects are a very mellow creative smoke,tastes like sweets,will grow it again ✌️✌️

Well she has definitely recovered, the frost is starting to appear.... Day 64 - Tuesday 18/12/18 - Added 3 litres of water, 6ml A+B - ph is stable. Day 65 - Wednesday - changed reservoir for fresh solution Day 66 - Thursday - added 1 litre of water , smells lovely Day 67 - Friday - added 2 litres of water and 6 ml A+B Day 68 - Saturday- added 2 litres of water and 6 ml A+B Day 69 - Sunday - added 2 litres of water and 6 ml A+B Day 70 - Monday - added 2.5 litres of water and 2 ml A+B Looks very frosty ,smell s very strong lemon

Raspberry Ripple Number One and Two (Flowering Plants) This week is the final week of Flower, I am noticing milky coverage on both plants and some amber trichomes as well. I gave the last watering today of distilled, dechlorinated water, with no P.H. Adjustments at 7.2 PH. I plan to harvest April 11th. Number One Pheno- The Number One Pheno is fully swollen and ripe now. The colas are densely stacked on the canopy. The foliage is quite over-faded, with some leaves being so dried out they crumble to the touch. I don't know if this is because the plant was in a bit too small of a pot or if my light was a little much these final weeks of flower. It is definitely ready for harvest! It's smells are still heavy in the raspberry candy department, with those hints of earthy leather on the back swing. Giving the number one pheno a very unique musk. The resin is greasy and plentiful! I can't wait to take this one down and trim it up to see how it looks in a jar! Number Two Pheno- The Number Two Pheno held out a little longer in its 5 gallon pot, it's a bit smaller of a plant. It's about 65% faded now but it's trichomes look finished! It's trichomes are very stacked out and coat this plant in a snow film! It may look a little boring next to its sister color wise, but it still sports some purple and pink hues under all the frost and I still think it'll be a worthy smoke as well! It smells of a raspberry wine but with that hazy fruity power. Almost like a strong mimosa strain. Clones- The larger clones in the 55 inch 2x2 MarsHydro veg tent, were starting to drink through water quickly! Mostly the larger number one phenos. Which would consume the entire dryback of their full pots in one day. I transplanted all the plants in the 55 inch tent into square pots (2 gallons for the Number 2 Phenos, 3 gallons for the Number 1 Phenos) this should hold them over for 3 to 4 weeks, so I can harvest and dry my flowering plants. I was unable to transplant the clones in the smaller Mars Hydro 30 inch Clone Tent, due to space constraints. I'll be feeding them worm castings and Down to Earth Crab Meal tomorrow. All the clones are looking healthy and look like they would be great candidates for flowering or mother plants. Tech Upgrade- This week I was able to purchase a Photobio Phantom Par Meter at a local grow shop's going out of business sale. With this tool I'll be able to get a dead on map of my light intensity and adjust accordingly. This will be great to have when setting up my new HLG Blackbird next cycle. I also got some 15 gallon grass roots fabric pots to try in the flower room next cycle. I also reached out to AC infinity and have an upgraded Cloudray S6 Gen 2 Oscillating Clip Fan on the way. My Gen 1 fan broke and would no longer oscillate correctly. AC infinity replaced it free of charge with the latest model. I think that about sums up this week! I'm very excited to take these plants down and begin preparing for next cycle. I was extremely lucky to get such amazing results of my first Pheno hunt. I can't thank Mosca Seeds and Tiki Madman enough for selecting such great crosses. It's hard to believe I got these two seeds as a freebie pack, with another order. I'm super thankful to have Grow Diaries to use and rely on for charting my week to week data, questions I have, and comparing my findings and results to other growers with similar strains, like @THCpapa grow of Raspberry Boogie! Thank you for checking out this week's entry of my growdiaries! See you for the harvest! -Starfish

Day 93 - Fattened up good, getting really heavy....posted pic of test bud.... vid of the led uv globe i got on ebay... works awesome.... highly recommend...... 😎 maybe 1 more week.... they smell delicious, not very strong smell.... just normal water in bottom drip tray, dutch master one gold ....next week, flush em and next sunday pull em..... idk.... Day 95 - had a good look at both girls and worked out a harvest plan...... i've started flushing/watering on the smaller one, and sunday (day 100) will be her last. i'll leave the larger one (she's still quiet white) for another week and re evaluate her fate next week. Day 97 - 4 vids .... looks good 😎 getting a 1600x Camera Endoscope USB Digital Microscope from ebay, see if it will help with harvest........ 😎

Adding video

they are all growing great and starting to get taller. a very robust plant and very easy to grow and maintain. they are getiing 1/2 the recommeneded dose of nutrients 3 times a week

Looking like she’ll be done very soon.... I’m giving her one week and then I’m coming back with my machete. 😎 Watered last night for the last time, she’s done receiving handouts 😂😂😂