Semana 5 y ya en prefloracion, he usado una enmienda orgánica que contiene muchos minerales y también apliqué lactobacillos, los que ayudan a soludibilizar el fósforo en el sustrato.

Boas growmies! Esta semana apareceram mais folhas amarelas mas penso que seja normal, penso eu, visto que já estão caminho da 10ª semana. O cheiro delas é fantástico! 17 Agosto - O gráfico representa a nutrição misturada em 2,5L de água da Royal Gorilla 1. EC = 1,55; pH 6,4 17 Agosto - Alimentaçao da Royal Cheese (2L): CE = 1,5mS; pH 6,3 -BioGrow - 3ml; BioBloom - 4ml; TopMax - 2ml; BioHeaven - 5ml; Activera - 5ml; Calmag - 1ml 17 Agosto - Alimentação Royal Gorilla2 e royal cheese 2 = Só água . ph 6.3) ----------------------------------------------------------------------------------------------------------------------------------------------------------- 21/08 - Alimentação Royal Gorilla #1 (2,5L): CE = 1,64; pH = 6,3 -BioGrow - 4ml; BioBloom - 6ml; TopMax - 2,5ml; BioHeaven - 7ml; Activera - 6ml; Calmag - 1,5ml 21/08 - Alimentação Royal Gorilla #2 (2,5L): CE = 1,33; pH = 6,1 -BioGrow - 3ml; BioBloom - 5,5ml; TopMax - 2ml; BioHeaven - 5ml; Activera - 5ml; Calmag - 1,5ml 21/08 - Alimentação Royal Cheese #1 (2L): CE = 1,36; pH = 6,1 -BioGrow - 3ml; BioBloom - 5ml; TopMax - 2ml; BioHeaven - 6ml; Activera - 5ml; Calmag - 1ml 21/08 - Alimentação Royal Cheese #2 (2L) - EC = 1,38; pH = 6,3 -BioGrow - 3ml; BioBloom - 4ml; TopMax - 2ml; BioHeaven - 5ml; Activera - 5ml; Calmag - 1ml

Fed once this week with a weak feed including top max. I use kombucha vinegar as pH down. Water from my tap is ph6.8-7.0 and 38-42ppm so I might start using calmag supliment as the ppm at my old house used to come out of the tap at ph7. 2 and 200-220ppm. I had 2 plants that didn't like the light at 30000 lux and started bleaching so I took them out. I've defoliated today day 35 as humidity was getting over 80% and also to let light to the lower bud sights. Pre flower pistils showed on some plants around day 33 (some still have none) so I'll consider this the end of veg start of flower. The kalimist indica pheno is still not showing pre flower pistils but is always hard to force flowering when it's not mature for some reason. It's always 7-14 days behind everything else 🤔

I'm really excited for her to finish..this is slowly becoming my favorite strain.. it's sativa dom but feels like a knock out indica (my ideal smoke)

Hi all 🧑‍🌾 Welcome to my 🍌💜👊 week update. Hope everyone keeping well and having a great week😁 Thank you so much for so much support on this bananas ride 🙏💜💚💜💚 Everything still going pretty well. Girls are developing and stretching like crazy. Athena currently Dec 14 90cm and main stem of Xena is not far of this height either. Unfortunately noticed first signs of calcium deficiency(my opinion) on Athena. Thankfully started feeding both girls with calmag weeks back but the dosage wasn't not enough for Athena. Started small treatment very next day on Dec 11 (see description below) I belive got my baby back on right track. Week 9 Dec 11-17 Dec 11 Watering was planned for Tuesday but due to calcium deficiency decided for a little treatment. Watered both girls with mixture of reduced content of growth nutes by 50% calmag at 1.2 ml per ltr. Reduced solution PH to 6.2 as apperently it's ideal for calcium consumption. Over period 4h I have watered Athena with 5ltr with couple runoffs. Xena 3ltr. Total of 7-10 leaves affected mainly at top colas close to the light. Dec 12-13 Purely observation and all looking well. Of course all affected leaves will progres of showing signs but good news is that growth and development booming and don't see any new leaves with symptoms. Very nice trichomes development in recent days and more and more colours. Dec 14 Second watering for this week 6.5l Increased calmag to 1.4ml per ltr and if still things will be looking well reduce to 1 or 1.2 on next feeding. Dec 15 Very nice respond on yesterday feeding. They definitely fatten over night and getting more and more frosty. Will need to remove some foliage soon as getting extremely bushy again. Dec 17 Xenas main stem top cola almost touched the light today. Tied her to left side of the cabinet, applied selective defoliation on both girls, tied few branches to netting and on the end of this busy day a third watering for this week. 7 ltrs beetwen both. Runoffs Xena PH6.4 Athena 6.3. Very nice week have to say. I belive that calcium deficiency on Athena is no longer a case here and both girls developing very nicely. Both are still stretching, buds are gaining in size and both girls started exploding with trichomes, Thank you all again for such amazing support 🙏peace and love brothers and sisters ✌️💚💜💚💜💚 Stay tuned for new week update 😉🧑‍🌾✌️💚

Semaine 12 : quelques moiavant la fin de la floraison.

She is flowering more and more each day and getting bigger than I expected haha. I have had to construct a makeshift "scaffold" around the tent so I can hang the light higher. The leaves are now slightly to big for the tent, so I hope she dosent get much bushier. Lots more pistels, and lots more growth, quite happy. # new tent coming 😁

01/03/22 Watered plants 2L of nutrient water each , assessed plants to determine what LST I'm doing, looks like these plants have potential to be monsters. Let's hope I don't fuck it up now. 01/01/22 Topped and defoliation was done, didn't water that day even though they needed it I wanted to give them a day or two from the stress they went through, watching to make sure they didn't wilt. Its paying of not listening to my ocd mind thinking I need to do something everyday, I didn't even want to top them, but they where looking very kushy type of plant. And I have trellis going in later two tiers. 01/08/22 Did a full water threw tested run off , it's sitting 800ppm above what I'm feeding, next watering straight 6.0ph water. Lights at 💯%

So guys , after 4 days o germination in 0.3L pots , i decided to move them for 10-14 days in 2L plastic pots . I use the BioBizz Light Mix Soil , first time also , but until now i ve used beside the feeding scheudle also Soil with High Levels of NPK , so we burned the plants and stoped them grow. A LUMIX 250 W vegetative light bulb keeps them warm and in 2 weeks abot , i will do tge last transplant in the 15L fabric pots . Gold Label Nutrients and Supliments , whole professional line in small bottles ( think some of them will last for years ). Visible Mistakes and worries : - dont know if the Light Height is good now - when should i start the spider-mites and other acarians treatment or pre treatment -what level of humidity should be inside in diffrent growing stages -I m so scared about Male plants , i have no experience at all how to spot them I had a hard week , hope you had a better one . See you later !

Not actually much to report here! Some slight bleaching to the main colas and clearly the leaves are being sucked dry of thier nutrients despite my feeding. Clear indication of her finishing up now, slowly the frost is appearing and the stank Fast Buds warns us all about is very much apparent! 😁 a beautiful smell to be welcomed to when ever I’m inspecting! I’ll give her water after her final feed today of nutrients, next week should be harvest week looking at the trichomes current maturity and pace of development. See you all next week with some much anticipated pics and smells! ✌️👊👍 stay safe and stay growing guys and gals! Much love as always 🙌🙂

I cut them down the night before the open houses started. The White Widow looks really good. I put them in darkness for 72 hrs, then cut and hung the whole plant, then wet trimmed hung for 18 hrs/day and put in paper bags for about 6 hrs/day for 4 days with a fan oscillating close by. Then in bags for one day, now they've been dry trimmed and put in jars. 50g for the White Widow and it looks great. I can't wait to smoke some.

flush time.

First put them in a cup of water then paper towel

Week 15 / 6th week in bloom :) One more week done, and even closer to harvest! Both my girls are looking great and starting to smell quite strong, I just love it!

Hermanos y hermanas no suelo hacerlo, pero he colgado un video sencillito solo para que miréis lo increíble que va la cosa. No acostumbro, pero os dejo ohir mi voz y en castellano y más... Dios Jah siempre en la guardia de todos. 420 siempre y que disfrutéis de más una semana muy buena y con la mejor vibración.

Ninguna observación más por el momento

Die Ladys beenden ihre 9. Lebenswoche ( Tag 63) und starten in die 10. Ich denke dass die Ladys in einer Woche reif zur Ernte sein werden, Sie Faden schön aus und auch die unteren Budds werden eine gute Qualität haben. Bin sehr zufrieden mit diesem Grow. Terpene sind super, Gasy/diesel/candy/ bubblegum/

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.