For weeks 9-10-11-12: It was now May 2020 and the ambient temperatures combined with no exhaust fans were raising temperatures to almost 30 degrees celcius. Way too much. So I rushed to find an oscillating fan and also a so-called inline fan with carbon filter for the smells that started to develop and for exhaust of hot air, trying to control too high temps.

I wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smile

@twenty20mendocino Ateam R&D Update ~ Let’s go day 91 an these girls are doing amazing! Looking nice an healthy we will let these girls recover for a few more days from the heavy defoliation and on Sunday we will be giving them the big flip , Let’s go flower!! Updates coming soon ! Sangria has been doing her thing , been drying for12 days now so Sunday we will be giving her the trim and jar her up, but updates coming soon yall!! Let’s have an amazing productive day as well as a great safe weekend, peace love an positive vibes to y’all Cheers an blaze on 😶‍🌫️💨💨💨💨

Signs of flower is becoming more noticeable on mental rainbow F1 have noticed more bud site development and more clusters of stigmas on the tops of main side branches as well as other setts of smaller side branches streaching upwards

After a hard week, you can finally see the effects of my efforts, if you look at the photo of my girl from day 43 and you compare it with 45, you can see the differences in the color of the leaves. The new set of fertilizers works and my darling seems to be happy, let it be like that all the time without any problems :) This is the first time that I am growing a photopyriodic girl, after changing the light cycle to 12/12 you can finally see her reactions. I am excited and waiting for the situation to develop 😍 By the way, she drinks 1.25 liters a day, 5 liters in 4 days. If I drank as much as she did, I wouldn't have liver anymore All The Best, You Lovely Girls Growers :D

Strain: Fast Buds – Mixed Photoperiod Trio Grow Type: Indoor Pot Size: 3 Gallon (x3 plants) Medium: Peat Moss & Perlite Nutrient Line: Athena (Bloom) Week 7 Update These three are doing excellent strong, healthy growth across all of them. Each one has its own little character, but they’re all showing great structure and consistent development. The canopies are even, the leaves look lush, and there are no signs of deficiencies anywhere. They’re taking their nutrients perfectly and stacking up nicely. Really happy with how they’re progressing can’t wait to see how these three look as flower kicks into full swing. Feeding 1.9 L Athena Bloom nutrient mix per plant (steady from this point). Indoor Climate Conditions Temperature: 24–27°C Humidity: 45–55% VPD: ~1.3–1.8 kPa See y’all next week! And don’t forget to use my discount code ISLANDT for 15% off your next order at Fast Buds!

Like how things are going👌🏾 Also i top dressed dry amendments on week 1 flower so i’m just feeding water with a tiny bit of megacrop and fish shit

Things going well

Day 67: Increase in flower size phrase has been finished now they about to go into the mature phrase. I will eighter flower them for 1 or 2 weeks depending on how it goes. Might only give them water with enzymen,regulator and humic for now on, that Is basicly flushing ( ec 0.7 ). or Ill just slightly feed them( ec 1.4-1.6) for a few times before I start flushing them( ec 0.7). Again I took the pictures before doing work on them ( watering and defoliation), so they look a little sad. I did notice that many of them got burned tips, indicating that they get more nutrients then needed. It does make sence since In this week and weeks to come, they need less nutrients and having a harder time taking it. Yet they do drink everything in 1.5-2 days, so they still do like to get nutes. Thats all for this week.

Since the second week I started doing LST on this strain. The plant grew very well, it was easy to train and had a very small amount of leaves, so I didn't defoliate it. Today I have done some lollipopping, taking off the branches that were too small. The buds start to grow in dimension and also start to smell. Also the resin production it's very good, if you try to touch the buds, your fingers will stick together.

KRITIC AUTO by KANNABIA Week #5 Overall Week #3 Flower This week she's is doing great no issues she's getting a nice aroma to her she's getting weight to her and you can see the trichomes on her buds. Stay Growing!! Kannabia.com KRITIC AUTO

Am Samstag direkt in die Erde gesteckt. Mit der Blumat Classic Bewässerung und etwas zusätzliches bewässern der Topfe durch ne Gießkanne, von oben nur mit Wasser. In den Blumat Tanks sind die Nutrients. Nach 5 Tagen kamen die ersten 2/6. Nach der Pause waren dann alle 6/6 von da. Voller Erfolg.

she slowly begins to sweat and also develops her characteristic color. i have not used any cropping or topping techniques, or defoliation, except that she will develop 2 head buds. i still don't have any uV or iR light supplement, just using a sanlight led and senmatic fl300 grow with adjustable colorspectrum and adjustable poweroutput. wondering. still in the same environment. all the pests have suddenly disappeared. i did not say any prayers.lol my mouth is already watering.lol

Day 64 the queens got some fertilizer 💪😃👍

End of Week 4 Trying to make sure the soil stays nice and moist to keep the microbes happy and letting the top 3 cm of the topsoil dry before each watering. . Turned the wattage up on my ballasts to 600W each. The ladies have gained some strength and they can handle the extra wattage. I also performed LST by tucking fan leaves and softly pinching and bending some of the bud sites also used some tie downs and topped here and there whilst keeping the stress at a minimal.

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.