2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Blue Mystic buds are starting to develop. Left lemonade nearing completion, right lemonade is several weeks behind left lemonade on bud development. Left lemonade has purple hues in the buds.

This week I’ve noticed a couple of fungus gnats on site. Not so happy, but I will keep my eye on the situation.

Flowering is developing well. Genetics Very very greedy in fertilizer... After a written exchange with the breeder, he recommended a mineral fertilizer, and not an organic organic one. The flowering is developing very, very well, but small spots have appeared on the foliage for no apparent reason. If anyone can give me the beginnings of a solution, I'm interested.

This week has been great ,today is day 78 and the girls are just doin their thang , 1 looks ready for flush already and the rest look about another longer then flush for them aswell,, can’t wait to see how these girls finish out over the next couple of weeks 😍!! Y’all keep them eyes peeled an stay tuned for next week! Peace love an positive vibes to y’all Cheers.💨💨💨

big and beautiful, she is starting to frost up!

Starting a new episode of the experiment, 16 flowers 4x4 models. Half organic using florian organic fertilizers. And the other half classically anorganic using canna nutrients.

**** Week 3 Veg - December 26/20 to Jan 1/21 ***** That is two weeks in solo cups and this will be transplant to 1 gal pots week. The girls are doing fine and not always perked up but I think a new home will help change that. They have been happy and noticed that when they were given a stronger feed at the end of last week they responded very well the next day and looked very healthy. Add that I am starting to see some purpling on the stem and I think they are ready for full on veg cycle to kick in. Not a lot of excitement her either Growmies😀 just to early with nothing go on other than making sure they are alive each day 😂😂 So for this week we will give the roots a new home, step up the ppm on veg feeding. Add in microbes to help the root development and keep the environment in the same 82 degrees and humidity in the mid 60's.......I am not supplementing with CO2 so keeping everything a little lower with feed, light, and humidity. They are growing veg well with the blue light and are very happy with the current 28" distance. Will maintain and let them grow into the light more until I see negative signs and then start raising the light with them as they grow. That's what I have to add at the start of the week.......see how the days unfold😀 Little more detail........ Dec 26/20, Day 15 Veg - Dry out day - Let the microbes in the media do their thing and start pushing root growth harder than what has been happening. Dec 27/20, Day 16 Veg - Gave the girls a little splash of the left over nutes from 1 gal girls two days ago to add a little oxygen and they can take the extra microbes and bacteria right now😀 - 100ml with Microbial Mass and Piranha @ 2ml/L = 650ppm and 5.7pH Dec 28/20, Day 17 Veg - 100ml each girl with the full line for this week. No other additives, just the IPP line = 700ppm and 5.7pH - This was also from the big girls.....I am thinking that they are ready for transplant so will give them a little high feed today. Dec 29/20, Day 18 Veg - It is transplant day.......we need to keep this party moving along. She may not have the root mass I am after but I am moving forward. - Prepared 1 gal pots with Mycorrhiza added to the holes. - Gave each girl 500ml watering with full strength IPP line for week 1 veg = 700ppm and 5.8pH - Root mass on these girls was very poor......there were roots.......keep stepping up the microbes with all of the girls!!!!!! Dec 30/20, Day 19 Veg - they were just watered heavier yesterday........dry out day today - think it is a CalMag only feeding tomorrow for all the ETS girls. Dec 31/20, Day 20 Veg - feed day today since it has been a couple of days. - 500ml with CalMag @ 2ml, Gold Storm @ 7ml, Sea Storm @ 5ml, Blackstorm @ 2ml, Vitamin B @ 0.5ml = 525ppm and 5.7pH - keeping the humic and fluvic acids going in, plus Kelp and CalMag👍 - the girls are happy and seeing forward movement everyday, looking for more aggressive though from the girls. Jan 1/21, Day 21 Veg - doing very well......like really well💪 - didn’t push them today and left them alone for watering, pots of course still had some weight and I didn’t expect them to need today. - Optic Folier Overgrow given today. Folier spray to give them just an extra boost since everything is otherwise dialed in👍 Finishing off the week very strong!!!!!! They have been looking very healthy and perky everyday. The roots are a concern and will be taking it easy on the nutrients for another week yet and keep up the microbes and beneficials. Will get them in line......my hope is too keep their time in the 1 gals down to two weeks max......maybe 10 days?? Looking very nice ETS.......you find yourself just staring at them and realize you just waisted 5 minutes😂😂😂

Day 9 - These plants are growing immaculately. Dark green, thick, healthy leaves, no stretching, they're growing like an absolute dream. Since removing them from the run-off catching orange cups, they have happily air pruned themselves and are continuing to beast out. 💪 I've only watered them once on day 5 so far. I've been warned by other growers that M3 is very easy to accidentally over water, so I am keeping that on my mind with every check. Day 10 - Noticed some pale spots on one of my seedlings. It's only on the second true leaves of one plant. Gave them a watering with 6.5 pH water Day 11 - Spots on one of my plants still there, taking up a little more space on the leaves. Still only present on the second true leaves of this plant. The plant appears otherwise happy and healthy. All three others are green and mean. 💪 Day 12 - Transplanted these little lovies into 1 gallon pots. Gave a light watering to just the outer ring of the pot with 6.5 pH water. My Step-Veg program for my California Lightworks Solar System 550 has been gradually increased to R80 W80 B99. First time transplanting anything outside of a seedling before, I was embarrassingly nervous. Made sure to get a ton of pictures of each one because I'm Mom af. 😂 Anyway - excited to see how many ladies I can score this round! Please give me at least one! 💜🙏💙 Day 14 - Closing out a beautiful week. I'm still unsure of what is causing the spots on one of my plants. I've gotten many suggestions of pests, but I've spent at LEAST a cumulative 4 hours over the past 2 days with her under the microscope, combing the leaves like an overprotective mother, and I have seen zero suspicious microscopic gang activity. As a precaution, I mixed some neem oil and dish soap and sprayed all the plants at lights out. Storytime was a big hit with the kids. 🤣💜

Trainwreck also doing great, plant is healthy in the same living soil setup like the others, only water no fertilizer. I did the same type of defoliation, removing fan leaves and leaves that grow to the center of the plant to get more light penetration. I also removed small lower branches and leaves to create a better airflow at the bottom of the plant.

She has been doing really well in such a small pot the roots are packed very tightly and am worried about root problems like root rot or lack of oxygen in the center being so condensed in such a small space i still have at least a week and a half to go before harvest hope it will make it to the end

- End of week 5 I topped them and they seem to react very well to it. Looking nice healthy and green , exept for the blue zushi on the right. It's growing really weird, but it seems to keep up with the other plants. I don't know if i will put it into flower. The 2 blueberry cupcake ( back row) on the right, are bigger then the rest. I bend one of them so it will be more even . Most of the plants have a different structure. They are f1 seeds , So there's a good chance you'll have different phenotypes Now i will train them a litte untill they are big enough, and then place a trellis net.

Die Kabul leitet langsam aber sicher die Blüte ein. Sie wächst schön buschig und gleichmäßig. Find ich echt stark 🤘🏻Sie riecht auch schon schön. Bisher gibt es nichts negatives zu berichten und das ist auch gut so. Die Palette an Dünger hat sich natürlich erweitert und BioBloom und TopMax sind jetzt auch dabei. Die Kabul nimmt den Dünger gut auf und verwertet ihn ordentlich. Bis die Tage ✌️

Strain: Chill Out OG F4 Harvest Day 64 Flower Day: 113 from sprout Medium: Fox Farm Ocean Forest Light: Vivosun VS2000 at 100% VPD: 1.5 kPa Light Distance: 12 inches Watering: By hand, ~oz daily Nutrients: pH Perfect Advanced Nutrition Grow, Bloom, Micro 2 ml / L, 1 ml / L big bud 30 inches

Harvested at day 76, after 72h of darkness. Very good yield! 3.3 pounds of dry buds + 1 pound of trim The 2 keepers yielded 366 and 342g of premium quality smoke. Very uplifting and energizing high :) Love it!

Hi liebe Community! 💚 Der zweite Strain von Good Times Genetics. Entwickelt sich wunderprächtig und es sind sehr schöne große und Breitgefächerte Blätter zu erkennen. Ein schönes Wuchsbild verreint mit einer starken Indica Dominanz. Dieser Genotyp entwickelt sich sehr homogen mit gleichmäßigen Abständen zwischen den Seitentrieben. Es ist allerdings ein starkes Blattwachstum erkennbaren, welches hoffentlich dann bei der Leaf to Bud Ratio nicht so übertrieben aus wuchert. Wir sind mega gespannt, wie sich die Pflanzen weiter entwickeln. Am Freitag, den 02.01.26 würde der Lichtzyklus auf 12/12 umgestellt. Die Wachstumsbedingungen im Growzelt sind weiterhin am Optimum und Stabil: ——————— 🌞 Temp: 23°C 🌚 Temp: 20 °C 💨 RH: 58% VPD: 0,86 kPa 😎PPFD: 330 mqm ——————— Stay Tuned! 💚

so I've been stalling transplant till I finish my set up and the plants aren't quite with me on this one. ran into some over feeding issues on both plants. one caused a lockout in Pheno 2 and the first Pheno is the dark green with my j on it. they slowed down a bit so I dropped way back on nutes. my little Pheno hunt is going good altogether and I'm learning a lot. thanks for staying with me. give me a like. 🙏🏿❤️

This was the first grow of my own, and it went pretty smooth overall. I'll update the outcome, tastes & effects after it has dried. growdiaries.com has been a great experience so far, very happy! 😀 Final dry weight was 2.4oz!!!👍