Can't wait to blazeeee Feeding💪 10/17 Water30L+Cleanse80ml+calmag@190ppm Ph6.3 Ebb 10.42/11.32/12.26/13 Clone 11.35/12.21/13.05/14 Average runoff ec1.5 Keeper 300ml per pot 10/18 Water26L+Flawless90ml+calmag@300ppm Ph6.3 Ebb 11.38/12.24/13.17 Average runoff ec1.2 ph6.5 Clone 12.38/13.56/15.20/16.10 Average runoff ec1.5 ppm750 10/19 Water26L+Cleanse80ml+calmag@200ppm Ph6.3 Ebb 11.12/11.48/12.24 Average runoff ec1.4 Clone 11.50/12.12/12.37/13.37/14.40 Average runoff ec1.3 ph6.6 10/20 Water30L+Cleanse30ml+Calmag@190ppm Ph6.4 Ebb11.42/12.22/13.20/14 Clone 12.40/13.20/14.20/14.45/15.15 Average runoff 1.2ec ph6.4-6.7 10/22 last light day 10/23 2 Pm Cut and hang Plan 21day at 23temp 55rh

💩Holy Crap Growmies We Are Back💩 Code Name FBT2307 Well growmies we are at 14 days in and everything is going great 👌 👉 Started to do some light low stress training👈 We all ready got , some Pre-flower showing already 👌 Lights being readjusted and chart updated .........👍rain water to be used entire growth👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

22.09.23- 84 день жизни замечательного растения ! Это финальная прямая 10 дней только вода , 4 из которых уже прошло )) 6 дней воды с пш, день засухи и харвест . Шишки очень нравятся , жду не дождусь когда попробую . Очень плотные и твердые. Для достижения большего количества трихом , за 2-3 дня буду царапать или раздваивать ствол в качестве эксперимента . 23.09.23-Продолжаю давать только чистую воду. Смотря на трахомы я вижу , что почти все облачные ! 6 дней до харвеста 26.09.23- 88 дней от прорастания Все еще получает только воду ! Сегодня я оборвал все веерные листья, чтобы растение получило стресс и выкинула трихомы. Завтра последний полив и 2 дня засухи перед харвестом . 28.09.23- 90 дней от всхода ! Вчера положил лед для лучшей выработки трихом и 1 день засухи 🙃

Lst on two plants. Leaving one to grow without any training method. She's doing good. D 31-received the mars hydro 2000w. Will be replacing the 1000w D 33-will let these girls grow without any more training.

In der 7ten Woche wurde Entlaubt um auf die Blüte vorzubereiten. Leider etwas zu wenig, also war am nächsten Tag quasi schon alles wieder voll gewachsen :( Insgesamt lag das Problem in der zu langen Vegetation. Danach lag der Fokus weiter auf Erholung und dem gleichmäßigen ausfüllen des Blätterdachs. Den EC habe ich nach dem beschneiden auf 1,2 gehoben. Voodoo Juice war nach dem Schema dann erstmal raus. Also habe ich auch den Bakterien etwas zufuttern gegeben, damit nicht alle sterben und ich dachte die komplexen Kohlenhydrate und anderen Zusätze aus Bud Candy kommen der Pflanze auch jetzt vorallem nach dem entlauben zugute. Einen Tag nach der Entlaubung habe ich wieder Foliar B-52 ausgebracht mit Break Thru zusammen, um Stress abzubauen und schneller erholen zu lassen.

Week 6. Im watering every 2 days. Fish shit and mammoth p every 3rd water. Rest just ph'd water. Alot of tricombs on the Purple Punch. Getting fat and juicy. Probably gonna ha4vest the Purple Punches in a week. GMOs finally coming along starting to fatten up and become a bit crystally. They still got 4 weeks or so left.

Lámpara: Bestva Quantum Board LED LM301B Sustrato: 2 Jiffy fino : 1 Floragard Especies: Charlotte Dream CBD - Auto Dark Purple - Purple Kush - Triping 25 - Gelato Ice Acontecimientos : 1. Las automáticas que me enviaron de regalía empezaron a florar muy pronto y van a dar muy poca producción. No vuelvo a poner automáticas gratis en mis indoor. 2. Las Charlotte Dream han crecido de forma brutal comparadas con las demás. 3. Se está empezando a hacer LST a algunas de las plantas, otras se quedaron al natural. 4. Luz 20h/día, nutriente y viento. Buenos humos

Making an addition to the diary to be able to join a contest

Can you believe this is just one plant!? She may only be 12 inches tall but she be over 20 inches wide. Looks like we're done with the webcam because this week the plant has grown to fill in all the space and swamp the camera. Did a few more LST bends as new shoots would start bursting through the canopy. It really seems to stunt their growth once they get pulled away from being the solo star in the limelight. My light is just two COBs so once things get off to the side you can really see the effects of the light dropoff. Plant still looks healthy A.F., I saw one leaf that had a couple brown spots on it but it disappeared beneath new growth so fast I don't really care to do anything about it-- I think my pH might be a little low. The little sucker is growing almost an inch a day, so I've been having to raise the lights every morning. I've decided to switch to flowering light schedule to 12/12, even though I've heard that a mature plant will have nodes with leaves that alternate and this plant seems to have them still coming out at the same place. To this end I've added 10x CREE XP-E HE Photo Red diodes to supplement my 4000K COBs. COME ON BLOOMS

Lots of LST and I started using a soil microbe hollar spray I made up for dr earth organic dry fertilizer.

day 49 just pure water phed down. it is day 51, morning before lights on, I might chop today. I use a zero water filter pitcher got off walmart. My humidifier is only a sonic wave. If you use regular tap water it will get gunky in a sonic humidifier. If you have a heat humidifier that boils the water the mist should be clean. These are the 2 types of water humidifiers. Researching best water filters this "zero water" also comes with a free ppm meter! yay! Other water pitcher brands; the water still has ppm. So water misted will still have ppm contamination. You can easily used distilled water only, and reverse osmosis. I just fill a 4gal office water jug with the pitcher and fill my humidifier when needed. I do not use a humidifier in my entire grow up to this point when I chop, I need the perfect rh or this whole effort can fail. growing up to this point only requires 20% effort, chopping now for the 2 weeks dry is this 80% of crucial determination, succeed. A lot of tasks are required today for a clean chop. I feel confident, observed my cross seeds with pink paradise and one with a slightly open caylex looks dark enough for successful germination. I figured 8-16 seeds may be created, 3-4 buds were potentially pollinated. Many growers don't understand when creating genetics with female/female there is a slight chance of herm. You lose 50% of your genetic function. Just water last 2 days, no humic no folvic, 1st time entire grow ha! There is a lot of cleaning to do, remove the trees, clean the entire tent and environment with bleach water, hacksaw. I am very proud of my grow. I have sampled 3 of the strains, dr. thunder legit made my lips numb but it could have just been the terps removing my skin cells from the sticky filter, no lie. This is my best grow to date. I did this for you, and to document hype or not under the best conditions I could give my plants, full max potential, although I wish I had more lights, I am not looking for industrial lol, this is how I grow. Learn a lot on youtube and podcasts there is many good content and I may dab into it. You can have a room full of growers and you have 1 seed in your hand, they will all tell you that their way is correct ;) and the best way. Oreo is stinky AF, Bridal is just amazing glad its massive, just not into sativa but the test seemed slight sativa with majority indica, pink untested, dr, thunder just a lip numming plant aha, its very nice, all 4 amazing appeal like advertised, and excited for next run. Either crosses from this or other copycat genetics. I will give you beautiful glamout shots today, the sizes of the plants, My guesstimate bridal 1lb, thunder 1lb, pink, 0.4lb, bridal 0.5, I am ambitious off 640w lm301h, and 4x 20w blurples daisy chained. day 51 4 chopped see you in a few weeks :D

Ya boii, you know the ABC sound! Playground Coming from the playground take it straight back to the streets You know what it is Well im Roro coming in third Nows about that time that i get heard, None of my rhymes have ever ever been heard Word to herb play tricks on ya mind, a come on Everybody here is real superb, and ill be right back with part 2 of rhis funky rhyme , B , ya... Yo red, i dont wanna flow no more man, yall go aheadz im just gonna sit back on this bad old beat Cuz this is what i do At the playground

Dec 8th Put 3 seeds into distilled water PH = 6.0 Soaked seeds for 18 hrs Dec 9th Put seeds into RapidRooter plugs Placed seeds into starter tray with cover and heat pad Dec 13th 2 of 3 have popped up ⬆️ today Dec 15th Looks like all 3 have germinated Started using fluorescent light pic above 18/6 schedule Dec 19th All three are up Two have there first set of leaves The third to sprout is just starting to make its first set of leaves Three for three 100% germination Dec 21st Planted into 4” baskets filled with hydro clay pebbles Dec 30th Second set of leaves Jan 3rd Planted seedlings into a 30 l res

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

It's happening! It's going to be a high of 32C/90F and so very sunny, so why not train them today? They're both 22 days from seed as of training start. #2 got FIMmed: So I was planning on topping one but ended up choosing to FIM it instead because it's lower stress. I have no idea what will happen now. I've never done either before. I chose #2 because it seems to be having an easier time producing leaves so far. I have a video of the cut. I used a pair of bonsai shears. I was really second guessing that I actually had to cut the leaves? I missed one, and it just felt weird to go back in and cut healthy growth, even though I'd already took off most of the newest leaves. I am not convinced I did this correctly because I don't understand what happens with the tiny new node I didn't cut. We will see. #1 got LST'd: With LST, I maybe should have done it sooner. The issue is the leaves are wide enough that they would be touching the soil if I really bent it like I was planning. I even undid it and tried the other direction just to see that it was even wider across. I propped a leaf on the tie and will have to adjust it again tomorrow once the crown has righted itself. I also read that it's not good to keep them in ties once they've gone into flower for autos because it stresses them, but with two plants I had LST'd, removing the ties just resulted in them eventually straightening the stems back out. So I'll not do that again for this one. I'm also a bit obsessive about measuring my plants, and that's harder with LSTing. I have a ribbon tape measure I use to measure the main stem length instead of the actual height of the plant.

Well, this strain is surely flowering for a long time, it's still stretching. I wonder if it will stop stretching and start fattening the buds this week... Anyhow, one of the reasons why I don't defoliate much is because the plant does that by itself. When the lower leaves receive almost no light (because all the light is blocked by the thick growing canopy) they start to send their nutrients to the top. When they are donez they just fall off on their own. You see, leaves are like solar panels AND nutrient reserves! Just make sure this process is slow and the leaves that receive light are still very healthy and green. If many leaves are falling or if they fall really fast you gotta make sure is not a deficiency or nutrient lockout! ... Again just water your plant with bloom formula (and a bit of calmag) to an EC that allows you to maintain the plant heathly and the runoff not to high (in my case 1mS/cm). Remember to alternate nutrients and pHd tap water. That's pretty much all left to do from here to harvest. Obviously if you see something wrong try to correct it. Day 66 update. I dropped something on my plant. It bended one branch very badly and severed some leaves and a bud :( Well, accidents happen. Luckily cannabis is a very very resilient plant, one might be surprised at how much this plants can take. Anyhow, taped everything together loosely, pulled the most bent branches upwards with a thread and I will wait for it to heal... If everything goes well, this branches should be even stronger than the others. Let's hope that's my fate! Update day 69. Although I have been feeding with 1.0 mS/cm once, then pHd water then feed again, the run off from today measured 3.0 mS/cm ! It's climbing still. I flushed again, until the runoff was at 1.5 mS/cm. I think I understand what happened, my theory is that the plant needs a lot more water now, but not as many more nutrients. So it takes the 2.5 daily liters of water, but it might only eat the nutrients in 1lt of water, so the rest stays on the roots and accumulates, raising the EC of the soil. So far, by checking EC on the soil once every week or two I have avoided nutrient toxicity and lockout. I think there are two options, either lowering the concentration of nutrients I feed it (so 2.5 lts a day will contain less dissolved nutrients), or spacing out the feedings with more intermediate waterings of plain water. I think I am going to go with the former, next week I will try feeding an EC of 0.8 mS/cm on 3L then tap water and so on from next week on. I prefer to underfeed than to overfeed, which is what I recommend for other boobs, it's better to have some yield than to risk killing the plant. We will get better with time...

Day 29 - Things going along nicely .... i trimmed down some of the bigger bottom leaves, i didn't have enough soil to fill the pots, and the plants are short, bushy, weird little things, that filled out the inside of the pots..... they are only 15cm tall, but the leaves are 10+ cm .... really weird little plants.... haha.... i decided to trim the fk out of one of the ones in the double pot, and practiced topping on her.... i'll cut her down maybe trimmer her of the pot, and focus on just the 2........ they seem to be still growing upwards, so i'm not in a hurry to put em into flower mode......... on the main one i noticed some neut deficiencies ... so i upped the neuts.... should be ok, i put a good heavy neut and it stopped the spots etc..... i think some bug or something got in the double pot and is livin in there, but he doesn't eat much, haha....... the main stalks are as big as my thumb and under each canopy is all the branches..... it's really strange, to go in under all the growth and see it all .... they look kinda small, but the entire under canopy of leaves is all full .... i'm moving the huge sized leaves out and pulling each branch up over them as they grow..... this is 21 THC and 19 CBD, so it's all the good stuff for medicine ... the smell is getting abit noticable and i'm hoping the exhaust fan arrives from china soon.... just got positive ventilation into a carbon fil. ... and daily air out........ i think i had the led lights too low..... so i'll raise em abit more and see if i can get em to stretch .... Day 30 -- added some video's...... daylight vids... worth watchin for a laugh ...... first grow, so idk what i'm doin, ... any help/comments appreciated Day 32 -- added some pics of the late night trim and LST,... idk looks ok.... Day 34 -- added a video...

So start of week 9 and start of my flush only using flawless finish at 2ml per litre every time I water 🍒🍒🌱🌱☮️☮️🍒 still using lst added another net to open her up and they have exploded with buds this ones defiantly a giver 🍒🍒🍒🌱🌱🌱coming to the end of week 9 had to put some more netting up to open these plants up there literally staked with bud from to to bottom opening them up has increased airflow through the plant after a couple of hours I could see this had helped they look geat I think