Everything seems to be going alright, one of my plants has extremely shiny leaves almost like oily, and some odd shaped leafs but I’m very curious to see how things go when I switch to flowering considering these are all random bag seeds! Have been trying to look for signs of sex but still can’t see anything yet!

Buena planta la dichosa Hulkberry. La técnica de doblar el tallo todavía no está muy conseguida. No me gustaba el aspecto que tenían esta vez. Contra todo pronóstico, han dado más cantidad de lo que parecía a primera vista. En todo caso los resultados son superables. La planta más pesada ha dado 22 gramos, y la que menos, 6 gramos de cogollos.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

4 week done now and progression feels to be paying off. I found a way to get a consistent pH that works for me and by the end of the week the girls started to really start to grow and move. LST almost everyday but most of the time I like to wait a couple days. But the plants at moving quicker. Up the ppm to 400 + and top soil with a giant mix of things which will run it course for the next 2 weeks or more depending on how I want to handle it. Week 5 looks promising. Hoping for some prominent stretching and some extra foliage. This time I ran 6.2 pH but next time will get a perfect run with 6.4-6.5 pH to really have them drinking. Started introducing more air in the room to enhance the co2 more as well. The circulation was good already but, always nice to have an abundance of c02

Day 29 they recover from topping and they are not stressed anymore. Will remove some yellow leaves. Day 32 performed the second topping today.

Everything looking good this week happy with the vegetative stage with the th Seeds original Bubblegum. Says they are an ibl strain so the genetics should be stable, which is less chance of 10 different phenos ( update me If I’m wrong here, still learning ) Day 54 - today I did the last defoliation and lst 2 days before swith to 12/12. Middle growth was insane so spread them out abit by screwing small screws in to the pot and using the as a tie down Day 55 - all plants seemed to have bounced back the day after and look exactly how I planned so far so good Day 56 - final day of vegetative growth all seems good, all females showing white hairs , have raised the lights and turned Intake to full to maintain humidity levels and switched lights to 12 / 12.

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Let’s go Day 36 !!! This week went real great, girls built up a lot of structure, and stayed super healthy ! Today they got fed just water phd at 6.5 ! They been really loving that balance! I also removed a lot of under trim since we are going into preflower , so had to shave up those legs lol ! It’s really great for them , the love that airflow , but don’t take off too much or you could shock your plants so be careful ! Can’t wait to see what these girls do this week ! Hope you all enjoy an are ready for another amazing productive week ! Peace , Love , an positive vibes to y’all Cheers LetsGrowwww!!! 😶‍🌫️💨💨💨💨

Que pasa familia, actualizamos de nuevo estos ejemplares de dutchfem, Casey’s rollex O.G. es una variedad 100% indica. Temperatura va de 21/23 grados ya sea de día o de noche. . La humedad anda entre 50% y como siempre el ph , ya que es de lo más importante,está en 5,8/6,0. . AgroBeta: 1 ml x L Growth black line , vía radicular. 0,2 ml x L Tucán , vía radicular. 0,2 ml x L Flash Root , vía radicular. 0,05 ml x L Gold Joker, vía radicular. . Hasta aquí es todo familia, espero que os vaya gustando el proceso tanto de crecimiento como la futura floración 💨💨💨 buenos humos.

Pictures would be better if I took her out, but I’ll wait another week for some glamour shots. Just chugging along. 1-2 more weeks! Stacked colas

Day 15 wow they are growing so fast Day 16 all girls got a flush we thought we might be putting to much nuts in it Day 17 I still can’t believe how fast these girl’s grow Day 18 all girls growing good Day 20 girls got over watered yesterday so taking the day off Day 21- last day of week 3 and growing great girls got watered today

5/12/2024 happy Mother’s Day to all the ladies and buds 5/15 super frosty sugar leaves. I can visually see them standing, that they are so tall 5/18 end of week 4

weather is too hot here , girls is not happy and not going well but they are resist 😁 we will see what hapəned to end ...🙄 6 weeks done 🙏

Amazin genetics. Had to clean the plant for more light . Plant react good to the lolipop ,lets see how much it will be effective . Again the ec rise to 5 -6 well my only hope is flahing every couple days . Had to rise the aquarium pump time to 30 min at the lights up . Still shows a leaf burn ,last day of this week..

almost ready to cut 🤤🌱 Day 76 1-2 days and proceed with the harvest I am aspetrating that the soil dries up well. we'll see when they give us these little girls of fustbuds

Buenos humos! ya pasaron a la segunda semana de floración, sigo aun con el foco de haluro metálico, se ven bastante bien en realidad para no estar con un foco adecuado para su ciclo actual. (el foco de sodio viene en camino) Los nutrientes los he ido dosificando según el desarrollo de las plantas, esta semana use la siguiente dosificación por riego: Riego 1: Acti Vera (1ml) - Alg A Mic (1ml) - Bio Grow (1ml) Riego 2: Root Force (1ml) - Big One (2ml) Riego 3: Rhino Skin (2ml) - Bio Bloom (2ml) Agua usada: Agua de llave + Agua purificada, mezcla de agua reposada 2 a 3 días según requerimientos nutricionales de las plantas. Ventilación: 2 ventiladores (1 giratorio) + 1 extractor de aire + 3 ventanillas rectangulares pequeñas de ventilación pasiva propias de la carpa indoor. Posiciones de las plantas: Royal Gorilla: Inferior izquierdo. OG Kush: Inferior derecho. Critical Orange Pounch: Superior izquierdo. Purple Afghan Kush: Superior derecho.

Week 12 and we're still not there yet! However, the ladies are growing thicker every week. #2 & #3 are growing so thick, they can barely support the weight of their own buds. Really have to be careful when handling them, the risk of snapping a branch by accident is way too high right now. The reason for that is probably due to the crowded space in the growbox, in which there isn't much airflow despite having the little fan running nonstop when the light is on. Looking at the trichomes they can all take a few more weeks, just have to be patient now! This is plant #1/3. Still thickening slower than the other ones, but she's pretty fine otherwise!

if i grew this again i'd make a mother plant, take clones and veg for short time and flip sooner then I did this round. Although people say lec 315 can be 18 inches from canopy (exactly where the platinum yeti was when finished stretching) the trichomes were just milkying up at day 66 of flower prob can run this one a little longer then i did to increase yield a bit more to allow buds to fatten out more and go more to amber trichomes. Smell: Gas ⛽ on jar opening. Ground up has Vanilla 🍪 perfume Taste: Movie theatre butter 🍿 fuel⛽ baked buttery vanilla cookies 🍪 Medical Effects: night time smoke! crazy couch lock, very sedating, helped me sleep😴 strong pain reliever. narcotic type high, slows thoughts down to clear focus calm. I felt glued to my chair more then any strain I've ever tried before.  Great stress reliever Notes: refer to this one as the "purple pheno" One of the most attractive smelling strains I've ever come across👃👀 when you smell it you want to smoke it. after smoking it you want to smoke more of it... but its pretty potent so this one is likely to end your night once you start on it!😎👌💨😴😴 yielded 23 grams of flower dry #3 green pheno- Smell: Lime with subtle skunk & burnt rubber Taste: Lime peels and burnt rubber Medical Effects: Very Sedating, stress reducer. slows thoughts down Notes: stayed green entire grow, yield 22.4 grams dry flower