Hey again my fellow growmies, Tomorrow being the 13th will be my last water change before i harvest on Sunday 17th. Then she would have been flushed for 14days and should be clean and nutrient free! The discoloration to her leaves show me that she is ready!!! Her smell is absolutely amazing I can't explain it. Apart from she smells like a candy store! Absolutely beautiful! Once harvested she will go into glass jars to be cured and then I can place a review and pics etc. Cannot wait!! Excited 😁 😄 💗 💖 😁 😄 Till the next time peace and love to you all 👍✌️🤜🤛🌱

Overall the best herb I've grown!

Week 12 . Tricomes are nicely starting to form everywhere. They are starting to look daam sexy! . The smell gets stronger and stronger and the leaves are getting real sticky to touch and when cut. Overall conditions are golden! And each time i change my water i get amazed by how clear it is!

Semana con nutriente de floración al 100%, se inició control biológico preventivo para la botritis, C4 de Mills y Bio Bud de GH. - Luz: Bestva Pro 1000W / 18 h/d -FloraNova Bloom G.Hydroponics: 0.7ml/l - Bio Bud G. Hydroponics 2.5ml/l - Armour SI G. Hydroponics 0.4ml/l - C4 Mills 1ml/l - Everest: 0.5 ml/l Control biológico: -Solution (bioinsecticida generalista: Beauveria, Metarhizium, Lecanicillium y Cladosporium + esencia de ajo y chile). -Acaridox (bioacaricida: Metarhizium e Hirsutella + esencia de canela) - Scamin (control escama y cochinilla) Cladosporium Herbarum -Bioprotection BD Plus (microorganismos bioestimulantes y bioprotectores: Trichoderma + actinomicetos + Lactobacillus) - Bioprotection TR + BSLIN (biofungicida generalista: Bacillus subtilis + Trichoderma y metabolitos).

Leaves are getting more yellow. Light burn? Turning the light down to 18 hours soon

11/1/2023 😂

31 Aug. I start the third week of my autoflower. The plant is growing well, but I noticed small yellow spots on the leaf tips, which seem like a mild calcium deficiency. This is probably due to the fact that so far I have been giving water with a slightly low pH, around 6, while the ideal range would be 6.5–7. At the beginning, to lower the ppm of the tap water (which was around 211 ppm), I mixed it with demineralized water. This brought the ppm down to about 109–140, making it harder for the plant to absorb the available calcium. Additionally, the soil I am using, Biobizz Light Mix, is lightly fertilized but does not contain enough calcium to correct this small initial deficiency. For this week, I will not add any fertilizers. I simply want to raise the pH to 6.5 and use water with higher ppm, so the plant can better absorb the nutrients already present in the soil. The lamp is positioned about 35–36 cm from the plant and so far there are no burn issues. Temperatures range between 25 and 30 °C depending on the day, while humidity in the room stays between 40–60%. The humidifier is always on because I keep the window open, but the dehumidifier is not being used, as it is not necessary at the moment. I also have a new oscillating fan arriving to replace the current fixed one, and I’m waiting for a regulable extractor (90–145 m³/h), which will allow me to control the air perfectly during winter if needed. This week my main goals are: Increase the water ppm without overdoing it Adjust the pH to 6.5 Keep observing the plant without adding any fertilizers Everything else seems fine and growth is regular. With these corrections, I expect the new leaves to grow healthy and free of spots. ==UPDATE 31/08== To control the calcium deficiency, I will administer an eggshell solution until I reach 300 ppm (I will boil some eggshells and administer the solution in tap water).

Well been a interesting week to say the least. The one problem plant looked worse than ever so had the experience of a lifetime trying to take that springy net off of the plants ! Don't know who's more traumatised me or the plants ! But got there in the end and did a big flush through the ph was out of ballance at the root zone reading 5.0 /5.1 should of done it a little sooner I suspect. Unsure what caused it as there both of the same res ! But here we are, frosts really starting to build had to supper crop the other more sativa she was looking for taking off through the roof haha! Hopefully that's the end of the drama. These are some tough plants thats for sure and the potential as you can see is huge as I'm only just a begginer in coco and autopots ! See you all next week

Day 71 I didn't label the plants so I don't know which is Sheryl or Candace anymore. I'm also not sure what I did different to have such varying results. One is thick, the other is not worth trimming. Based on camera zoom photos it looks like we're at 10% amber trichomes, which means it's likely the last week. Lesson: Label each plant, take better notes. I bottom watered 6.5ph 1tsp of Terp Tea Bloom in 15oz each. Possibly the last watering. I ordered a digital microscope so I can be sure on the trichomes. Day 72 Microscope came in. It's not great but it was $15 so... anyhow I could see that the chunky girl is ready with high amber trich count (30%) upper plant and about 10% in the lower buds. I put her in dark and will chop tomorrow. The skinny girl that pissed me off last week has about 5% amber trichomes so she got fed and watered. Additionally, she stacked up and put on some weight. Nothing too crazy but it's noticeable. I'll give her another week or two. By the time the chunky plant is done drying (HerbsNOW dryer) and in a jar, the skinny plant won't be skinny anymore and she'll be ready to come down.

Almost Ready to incorporate fish into the res to feed the Few roots hanging out! Cover crop coming in nicely! Seedlings looking super happy! Can't complain so far so good can't wait to start Feeding fish!!!

Realmente muy feliz con lo obtenido, 307g en 0.8me me parece genial, estas flores tienen un aroma genial entre el típico haze mezclado con cacao, un efecto muy agradable y confortable, rica al fumar y muy poco picante gracias al lavado de raíces con flawles finish 👌👨‍🌾🏻 Totalmente recomendable esta genética de royalqueenseeds muy agradecido por la oportunidad 👍👌👨‍🌾🏻

Day 44 -

You rated, we passed it to Serious Seeds with Adam iLL!

Time to spill it! Serious Seeds just got their rating️ from the growers over at growdiaries.com—total respect for their killer work in the grower's world! #SeriousSeeds #growdiaries

🍼Greenhouse Feeding BioGrow, Bio Enhancer & BioBloom ⛺️MARSHYDRO The ⛺️ has a small door 🚪 on the sides which is useful for mid section groom room work. 🤩 ☀️ MARSHYDRO FC 3000 LED 300W ☀️Also special thanks to VIPERSPECTRA P2000 (200W) & XS2000(240w) LED growlights 🌱 Ganja Farmers

These two plants have given me the experience and the desire to do even better! Next genetics I thought I would bring another pure Indica...

Did a top dress of some grow powder dry nutes from botannicare. Just giving her rain water for now. Again—- temps, humidity, and clouds have been aalllllll over the place. One day it’s 86 and the next is 66 with nights in the 60s or 40s—- humidity and rain have been 100% down to 50%. All within a week.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 58 - As you can see, buds are looking full but still looking like they want to go a bit longer, checking the trichomes frequently and seeing a lot of cloudy.. think i saw the first few ambers too. Any how, we shall keep going. Flush time soon. Day 62 - Looking good, smelling better.. really fruity and sweet. Seems ripe too. Feeds lowered and a little flush. Buds are rock hard, very dense and sticky. Looking forward to harvesting. She will be ready by day 63, but I'll see weather or not ill let her go longer. Stay tuned. Day 64 - trichomes ready, little flush and shes ready for the chop. Beautiful smelling strain and easy to grow. Very hard dense gassy and sweet buds. Once dry and cure, will be back with a little update.

I hung the full plant upside down for 24hrs. I then hung the individual branches. I left the branches big and I didn’t trim the buds as I wanted to dry slowly. They hung in the dark room with temperature at 21 degrees and humidity at 58% for a week. I then removed the buds from the branches and placed them in a drying net for another few day before jarring. Smell isfantastic.