Heute wurden die Sämlinge eingepflanzt in den finalen Topf von Terra Aquatica, Cultimate, 15 Liter. Die Steinwoll Würfel gieße ich dann nach 3 Tagen mal,das System habe ich eingeschaltet. Die Sour Jealousy von Fast Buds ist ein Ticken größer wie die Orange Bud von Dutch-passion. Mal sehen wer das Rennen macht.. ppfd liegt bei 215, bei 20 Stunden Licht haben wir einen DLI von 15.48.

Dane, We all should help one another. Human beings are like that. We should live by each other’s happiness - not by each other’s misery. We don’t want to hate and despise one another, share the Joint. And mother earth is rich and can provide for everyone. We can Grow enough Happiness, In this paradise, there is room for everyone. We only exist to bring joy into the world and The way of life can be free and beautiful, but we have lost the way. Grow High and Give the world A smile. At the end we own nothing more then all our memories, lets make them amazing for everyone, nothing to loose only everything to win. A last kiss goodby, a second one, softer and long as a sign, that you are woth it. That Everyone worth who loved and give. Enought Hippie Talk, now have a nice day and an even better grow, thx for watching by. ((From Seed 🌱 week report: (58 refil 10L

SUMMARY: Switched up to Mid Bloom strength nutes this week. I have also started adding 1 tablespoon of organic blackstrap molasses per every 5L. Though some argue this is purely for use in soil to feed the bacteria there is argument that it also acts as a bloom booster due to its active ingredients. She is fattening up nicely though not many tops and so I need density from these. DAY 51 ------ Sunday 21st February 11:30 I made 5L of early bloom nutes at a pH of 5.8 and 5L of mid bloom nutes at a pH of 5.6, both made with tap water. Sunday 21st February 18:30 I fed the NL 1.2L of early bloom nutes which produced roughly 300ml runoff (25%). DAY 52 ------ Monday 22nd February 11:45 I made 5L of mid bloom nutes with rain water at a pH of 5.9. I am adding half a tablespoon of molasses to every 5L now by adding just enough boiling water to liquify the molasses and then pour it into the feed. Monday 22nd February 18:30 I fed the NL 1.4L of early bloom nutes with molasses which produced roughly 335ml runoff (24%). [22°C/55%] DAY 53 ------ Tuesday 23rd February 18:30 I fed the NL 1.4L of early bloom nutes which produced roughly 400ml runoff (29%). With the dehumidifier set to 50% RH it seems to keep it at around 50% RH though the temps between 25-30°C. If I turn the dehumidifier off the RH climbs to 60-64%. Removing more leaves will lower the humidity and I do pluck the odd one every day that is blocking light (I rotate the plants daily 25° per day to ensure all sides get a fair share of light). [25°C/50%] DAY 54 ------ Wednesday 24th February 18:30 I fed her 1.4L of early bloom nutes with molasses which produced 200ml runoff (10%). The temps have risen to 30°C due to the dehumidifier. It is currently set to 50% RH which keeps the temps around 28-30°C and the RH at around 38-40%. I have increased the setting to 65% to see if that helps lower the temp and increase the RH up to 45-50%. [30°C/40%] DAY 55 ------ Thursday 25th February 11:30 I made 10L of mid bloom nutes with rainwater and molasses (level tablespoon per 5L). 5L at a pH of 5.9 and 5L at a pH of 5.8 Thursday 25th February 18:30 I fed the NL 1.6L of mid bloom nutes which produced roughly 610ml runoff (38%%). [22°C/55%] DAY 56 ------ Friday 26th February 11:30 I made 10L of mid bloom nutes with rainwater and molasses (level tablespoon per 5L). 5L at a pH of 6.0 and 5L at a pH of 5.6. Friday 26th February 18:30 I fed the NL 1.4L of mid bloom nutes which produced roughly 420ml runoff (30%). [22°C/55%] DAY 57 ------ Saturday 27th February 18:30 I fed the NL 1.4L of mid bloom nutes which produced roughly 410ml runoff (29%). [22°C/55%]

No issues so far.

Letzten 7 tage unglaublich an masse und höhe gewonnen echt atemberaubend das anzusehen ,hab das zelt gut voll bekommen mit wenig plants bis jetzt alles super. Habe heute meine neue Led lampe bekommen bin positiv begeistert.Habe 600watt durch eine 1200watt ausgetauscht.

We start week 3 of bloom!! Goofiez 2 amor compound genetics it’s the champions!!

Lot of eain

Everything’s fine so far. The plant is looking great and takes up the space it gets. Stretch is just perfect and very manageable.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Erstmal zum Trocknen aufgehängt. Ergebnis kommt noch.

Week 8 - we are getting so close. The bud smells so amazing. It’s a citrus earthy smell that I can’t wait to taste. The weather is cooling down and she seems to like that. I wish I had more hours of light, but she’s as big as she is going to get.

The end of the 5th week of flowering. Just like the other 2 weeks before this was a cold and rainy week. The buds are really struggling to grow/fatten. they grow thought, but slowly because of the colder climate. Because of the struggle and colder climate, they don't drink as fast as they used too, so I give them less to affoid overwatering. I might have to lower my EC as well to make sure they will keep drinking properly.

Once I got the 200 watt light things took off

Some trips problem

Dried and now in Curing