2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Week 9 Didn’t do anything this week except water the lady’s. My telescopes batteries died so I can’t take pics of the tricomes. Rest assured they are nice as hell. They are a massive mix of colour. You see purple, cloudy yellow and amber. Super excited to puff on these nugs!!

Start of week 5 today, everything seems to be going great! The Gelato OG is by far the biggest plant of the bunch so far but they all seem to be taking well to LST, HST , Topping & defoliation! Will be going in the flower tent as soon as the other ladies harvest so they will probably have atleast 2 to 3 more weeks of veg!

She's doing so well not doing much but feed her and got her a wee defold.

**400 Gallon** fabric pot (Not 80 Gal) Yes that’s right, no nutrients in the water. The soil is rich. So right now we are just getting her established with her new fixers. (Field Peas)

These are doing pretty damn good for the most part. I figured out with the green buzz the only thing in bloom that raises ppm is the base bloom and the more PK which is good to know. Also been using lower ppm for the autos and they seem healthier than usual. These pics and the video were taken on Day 36.

This girl has been one of my favorites. Pineapple express is true to it name looking like a pineapple once it fades the yellow, and the green are very beautiful together smells gassy with pineapple busting out of every tone

Both plant are doing well for the lil that is being done....going to take one or two cutting in the upcoming week so they can live up to the mothership name n allow more vertical branches

Oh Ya Baby!! I’m so impress

This girl is exploding in the last weeks. The buds are getting so thick finally and our expectations for harvest are getting better than last week. We are not feeding her a lot as we already want her to suck up everything the soil already has. 👇 The weather here is also pretty humid, she is not showing signs of water lack. I would love to hear from anyone opinions on the maturation point. How ready is she? 🙌 This is our first time with a lens so we can't really tell just by looking at it. hope you enjoyed, see you next week! 😛😁 18-10 leaves are looking really bad, looking for some advice

Day 0. I got new light and tent from Mars Hydro, so maybe ill make it in time for two rabbits ;))) Bubba Cheesecake challenge started ! Light is great, has dimmer, in user manual youll find distances, so TS1000 is great grab for starters and CHEAP ! Again was imaging way lower quality from photos. Tents are great help too, plus i really think they are great bargain for price. Last couple years without any major issues. Happy Growing !

💩Holy Crap Growmies We Are Back💩 Well growmies we are at 49 days in and everything is going as good as it can👌 Afraid she's had some major issues but that's just how it goes folks 😉 👉 Shes a short chunky little plant 👈 We got some very pretty colors😍 👌 She's got some odd colors kinda like tiger leafing,😉 Lights being readjusted and chart updated .........👍rain water to be used entire growth👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

OMG I am beyond excited to talk about my Medusa Girl in her first week of flowering. Let me tell you, she's an absolute goddess in my garden. She's been loving the autopots just as much as I have. I mean, who wouldn't love having their water and nutrients taken care of automatically? It's like a dream come true for any gardener. And my Medusa Girl seems to be thanking the gods for it every day. Speaking of gods, have you ever heard of Medusa? I can't help but make a little joke about it, considering the name of my plant. But instead of turning people to stone, my Medusa Girl is turning heads with her perfect bud formation and gorgeous growth. I'm in love with every little thing about her, from her deep green leaves to the way she seems to be always praying to the gardening gods for a bountiful harvest. It's like she knows how much I care for her and wants to give me the best possible buds in return, or this is what i imagine is going on hihihihi <3 <3 <3 As always thank you all for stopping by and for supporting me on this journey, i am super passion about growing and fell blessed to have you all with me on this new journey <3 <3 <3 Genetics - RQS MEDUSA F1 Ligth - LUMATEK ZEUS 465 COMPACT PRO 
Food - APTUS HOLLAND 
 
All info and full product details can be find in can find @ https://www.royalqueenseeds.com 

https://aptus-holland.com/
 
https://autopot.co.uk/ 

https://lumatek-lighting.com/ With true love comes happiness <3<3<3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3<3<3 <3 <3 <3 Growers love to you all <3 <3 <3 Medusa F1 Medusa is a true F1 hybrid created from pure, inbred cannabis lines. She boasts uniform grow traits, mouthwatering aromas and flavours, and plenty of potency. If you're looking to bring stable, elongated plants into your room, tent, or garden, look no further. Mouthwatering Aromas, High Potency, and Mid-Size Plants Though she won't turn you to stone like the mythical goddess after which she's named, Medusa F1 is bound to get you plenty stoned in her own special way. Combining genetics from inbred lines deriving from Sugar Magnolia, a thick and sweet indica, and American Beauty, a fast, fruity hybrid loved for its well-balanced, positive high, the result is an autoflowering F1 variety that produces fresh flavours, a unique cannabinoid profile, and large yields. A very aromatic cultivar with a vibrant concentration of terpenes bearing notes of fresh mint alongside an upfront peppery kick, backed up by hints of fresh fruit, berries, and tangy fuel. Her thick, frosty flowers consistently produce high levels of THC and CBG, as well as high concentrations of myrcene, ocimene, farnesene, and caryophyllene. As a result, Medusa F1 has strong effects that relax and stone the body from head to toe (brought on by particularly high concentrations of myrcene and farnesene) while motivating the mind with an uplifting, creative, and motivating kick (thanks to high concentrations of ocimene). Medusa F1 seeds produce elongated autoflowering plants with long, strong branches and well-spaced internodes. Plants regularly grow up to 80–85cm tall and boast a fast flowering time of just 42–45 days (72 days from germination to harvest). Thanks to her genetics, Medusa F1 produces plenty of thick flowers with big, swollen calyxes that make for very easy trimming. Medusa F1 is very stable, handling stress and unfavourable growing conditions with ease, though she is somewhat susceptible to Botrytis, so make sure to keep tabs on the humidity in your grow room/garden

This week will be the last one for these babies, already started flushing them with water. Plan to continue so for 2-3 more days until reach around 700-600 ppm and Bob is your uncle. It was my first experience with auto flowering strains. It gives much less amount of products comparing to photo, but less stress as well, easy growing, plants are quite are unpretentious. Let's see how potent they will be.

She’s getting frosty and dank 👃🍪🍊🍓🍰🍌🍒🍇🍭👃 Day 29 almost half way , happy with all the phenos so far all frosty so many terps .. started feed the canna nutrients plants canna pk1314 .. 2 flush’s Got the remo plants all most on the proper Ec again

Simply put I am so very pleased with the way this plant has performed my only worry is how to properly dry and cure this magnificent specimen

01/09/2022 Finally time to flower, and all girls went into flower room at lights out.The males will be next week, don't want to put them in to early so I will carefully check every day and separate before they open their flowers. I'm guessing week 3-4 I Will select two branches on each female to selectively pollinate. 01/11/2022 Blue Matter #5 (update #5 culled male) repotted with lemon og seed Will continue in her own evolving diary. My hope is to eventually graft different cuts for a multiple variety mom plant, I can have 6 total plants And would be nice to be able to maintain only one mom.

Very ple4ased with this plant so much so that she will migrate into the other tent where she will have more space to flourish, I am growing in a mixture of biobizz allmix plus coco and its going well . She is only 34 days old from seed and the growth has been fantastic. I must be doing something right for a change !!!