Day 106 12/25/2021 being of a new week and this will be more than likely the last week this lady gets any nutrients thinking Wednesday. After that will start flush with collected rain water. After checking trichomes they were about 85% cloudy about 10% clear and 5% amber which is right where she should be since the end is coming fast. Day 110 12/29/2021- Started flush today and will continue to just feed rain water until next Wednesday. Will cut her down next Friday 01/07/2022.

~ FastBuds PAPAYA SHERBET Photoperiod~ Well here we go on another journey together through 'Canna-land' and this one's kinda special... forget that, it's VERY special because this is FastBuds newest surprise for the community, a FEMINIZED PHOTOPERIOD STRAIN!🤯 I was fortunate enough to score these seeds, of which only 1000 were available and can't wait to see what this strain can do! This Papaya Sherbet, according to FastBuds, is a hybrid with a 9-10 weeks flowering period. For a more detailed and accurate description of this strain the following from FastBuds says it best: "Combining the massive stature of Papaya (Oni Selection) with the strength and resilience of one of our best keeper cuts (Sunset Sherbet), this strain develops into a big, expansive bush adorned with numerous bud sites that later transform into a generous harvest of medium-sized buds. Papaya Sherbet flowers deliver a signature flavor of premium cannabis with subtle citrus undertones that emerge upon inhaling. During growth, her aroma makes for a sweet yet pleasantly bitter fragrance, giving you an idea of what the smoke will taste like. Notably stress-resistant, Papaya Sherbet is a great choice for growers working in challenging environments. She is very forgiving and rebounds quickly from any adversity, allowing growers the freedom to experiment with confidence that she will take everything like a champion she is. This strain embodies resilience, flavor, and abundant yields in every grow cycle." Sounds like an epic strain and I personally cannot wait for this lady to strut her stuff!😍 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ The Setup: This is going to be an outdoor grow, but I have started the Papaya Sherbet photoperiod indoors as our weather is still a bit too chilly to put a newly sprouted seedling outside (nighttime temp's dipping regularly into the 40's℉). The plan is simple... let her grow inside under a 19/5 light schedule until the nighttime temperatures are in the mid 50's℉, which shouldn't be long. After which, she'll be moved outside and transplanted into the soil which I have already setup and inoculated with beneficial microbes from BioTabs and slow release dry amendments from Gaia Green. Once she's established herself outside she'll be given periodic top dressings of Gaia Green 4-4-4 and 2-8-4 along with worm castings and Compost Tea's. Her grow area is approx. 5'x5' and I have posts and a trellis net set up already for when she gets bigger to aid in training her. Let the fun begin!🤪💚 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 9/4- The 4th week of flower is here and the FastBuds Papaya Sherbet photoperiod is looking fantastic! She's loaded with flowers that are all beginning to get frosty! 9/6- My daily watering campaign continues with me giving the Papaya Sherbet approximately 5 gallons of well water from the garden hose. The flowers on the Papaya Sherbet are stacking away and the frost continues to accumulate! 9/8- Today, before watering the Papaya Sherbet, I did some needed plant maintenance, removing a bunch of old dead leaves, along with a handful of shade leaves that were yellow. 9/10- Well with four weeks of flower behind her now, the Papaya Sherbet photo is almost halfway through with another 5-6 weeks to go. I only hope and pray🙏 that the weather cooperates and stays cool and dry! Thank you for checking out my diary, your positive comments and support make it all worthwhile! 💚Growers Love!💚😎🙏

Looking good. Couple weeks left!

WEEK 16 - (06/13/2021 - 06/19/2021) Week 8, Day 50 - FLOWER: 06/13/2021 After inspecting the trichome development for the last 7-10 days or so, I’ve decided that today would be the ideal time to begin flushing. The American Pie #1 does not look like it’s ready to begin flushing quite yet, as she appears to be about a week behind in maturity. Despite that, I’ll still begin flushing her today too. Recycled water: 70ppm, 69℉ + pH Up: 7ml Mixture: 95ppm, 6.4 pH, 69℉ ppm/ppm = runoff/soil JB#1 - 895/800, 6.1 pH AP#1 - 529/434, 6.5 pH AP#2 - 365/270, 6.7 pH GC#1 - 624/529, 6.3 pH -------------------------------------------------------------------------------------------------------------------------------------------- Week 8, Day 51 - FLOWER: 06/14/2021 Did a trichome check on the plants today. Spent more time with the Jelly Banana to better determine her maturity level. She actually looks like she is ready to harvest today. I’d say trichomes are 75-80% milky, with an amber trichome here and there. I’m going to try and push her through at least one more low-ppm watering and test the runoff. If I start seeing more amber trichome development, I will cut her down. -------------------------------------------------------------------------------------------------------------------------------------------- Week 8, Day 54 - FLOWER: 06/17/2021 Recycled water (8 gallons): 69ppm, 5.8 pH, 70℉ + HydroGuard: 8ml + pH Up: 3ml Mixture: 82ppm, 6.0 pH, 70℉ ppm/ppm = runoff/soil JB#1 - 688/606, 6.5 pH GC#1 - 602/520, 6.5 pH AP#2 - 633/551, 6.9 pH AP#1 - 685/603, 6.5 pH -------------------------------------------------------------------------------------------------------------------------------------------- [SUMMARY] I expect this to be my last full week of flower. All the girls are looking ripe for the chopping. The aroma that bursts out of the tent when I unzip it every time is heavenly. American Pie #1 is still about a week behind in maturity, but she will get cut down with the other plants regardless. Jelly Bananen started to turn a lime green color so she'll probably get chopped and hung up in the next few days when my second tent arrives (4x2 AC Infinity). That wraps up this week! See you ladies and gentleman in the next entry. 🙏

6/1 I'm anxious to get my plants in their final homes. Another 90 degree day today and then it will be cooling off with some showers. I plan to take advantage and utilize that to make the transition. I have some small burns on a couple plants bit considering the weather we've had its nothing. Temperature swings 50 degrees. I hope these girls do OK outside. I was tired and stoned last night and I accidently topped a plant I had already FIMed. Oh well. Transplants soon. Supports screwed in after the heat. Plants have been handling this weather like a champ. Big thick healthy stalks. UPDATE PUT THE PLANTS IN AROUND 730. Cutting down on light hours I'm poverty close to where we are. It was 95 today. Plants have suprisingly little burning. My fan was taken so they go without that tonight too. In the next few days it's going to cool down and that's when I'll transplant. Only thing left before transplant is sanitizing trellis and supports. Easy peezy. Especially if it's supposed to be in the 50s. And people dent climate change lol. I'm hoping that all this adversity makes my flower a higher quality. I'm glad to have the quantity to pick the best phenos for the BIG POTS. Im trying to stay excited but it's just so dumb hot. Oh well. If the plants can do it so can I. I'm planning to give them some kelp me kelp you next watering and may start some light organic nutes. 6/2 Phone doesn't like uploading my videos. Broke record high temp yesterday 95 and tomorrow we will break the record low 50. Plants withstood the heat well and are doing great. Transplant soon. They were a little light but I held off on the water. Plan on it tomorrow. May transplant tomorrow. I have several videos that haven't uploaded. I thought they did but I was wrong. Oh well. UPDATE: Forgot to water. Some were light and others could've held off. Did the half a powerade bottle ahain. A litt ke less on the heavier ones. However I'm looking to transplant into final homes tonight and into this weekend. I sanitized a fifty and a forty so my bags and two pots are sanitized. Cage is sanitized and ready and pallets are sanktized and in place. The 40 and 60 fit PERFECT in the small space left from the pallets. Plus the plants always lean the opposite direction so things should go smooth. Only thingvleft is sanitizing stakes, bamboo, plastic garden stakes and all the other plastic training stuff. I'll just soak it while I transplant. Gotta mix the soil first too though. UPDATE. Heat went higher than expected and hit 90. I figured I'd go back around three and screw in suppirt stakes and begin getting everything ready for transplant (i.e. supports, mix soil, sanitize trellis) before I transplanted tomorrow MORNING was what I had planned. Unfortunately we got a huge thunderstorm with a shot ton of rain and it's gonna continue throughout the night. I think this is God's way of telling me to take a break. I can get up early and get those support stakes screwed in. Then when I get back from the wife's doctor's I can do the transplants. I'm trying to break the labor up into manageable chunks. Looking at my other diaries I can see that my plantscare fine in their three gallon homes. It'd a little disheartening seeing such big clones that I started so much earlier on the other diaries. However I always was fighting SOMETHING (usually more than one thing) and last year was a shit show. At least this year I stay away from anyone else's cannabis any clones and I'm extremely cautious. I've seen my cousin out perform me multiple times with healthy plants like this I'm excited to see how it goes. I really needed the break anyway but tomorrow morning the stakes will be in. Hopefully they'll be outside. 6/3 Broke the record for low days after breaking record for highs. I saw it 94 yesterday and the day before. On the way to my grow at 6am it was 44 degrees and it's not warming up much. Fifties at best. Rainy. I left the door closed. The big door on the otherside got opened as the other end of the garage neededcto he used. If the rain let's up I'm going to set up the grade stakes so I can transplant in the morning and throughout the day. I think I'm going to light dep a couple of them and leave them in the 3's. We'll see. UPDATE: At 12:30 the temp today was 44. Yesterday it was 94. I can't believe this. Plants are doing great though and are in the garage under lights for the day. The BIG door was open part of the day. Didn't take any pictures (or even enter the grow area) as I sanitized the last 40 gallon pot and a bunch of grade stakes. I drilled the grade stakes to the pallets in the front. The first row is done. Just need to sanitize the plastic trellis itself and finish attaching the other grade stakes. I don't need the fill trellis up by transplant. I just need the bags to not knock over and have SOME support. I'll be back over there either to tonight to put more supports in or tomorrow when I actually plan to start transplanting. I'm getting excited. After dealing witb the stiff that I sanitized I didn't dare go fuck with my plants so I looked through the window. I'll finish sanitizing the net tomorrow. These plants look great. I'm wondering if doing the two diagonal I poles with zipties actually works. It would save me some kone for sure. I'll definitely update in the morning. 6/4 It's warmed up to a balmy 44 degrees. My plantsxare in the garage still. Weather sucks this whole week but after this heavy rain it should be perfect weather to transplant these girls. A little to chilly but it will warm up and I need to get this shit done. I'm trying to do to much at once. I need to just out dirt in bags and get them outside. I'm glad I waited until after this craziness. Breaking temp records for high one day then breaking it for low the next can't be good for the plants. I'll update what gets done today. I may mix soil or I might wait until tomorrow. It will let up some then. Plants still look fabulous 👌 6/5 50 degrees still raining like hell. Rain until Friday. Plants are inside under the onecworking light. Lots of indirect light through the massive windows though. I'm planning on mixing soil today and tomorrow and getting bags and pots half filled. Just trying to break the labor up into manageable pieces. It's probably a good thing I didn't put them in their huge containers before all this rain. UPDATE: MIXED SOIL 1/3 of each (FOX FARM OCEAN FOREST, HAPPY FROG, AND ROOTS ORGANIC 707). I prepared I believe 11 containers for transplant. I need to sanitize two more containers and I'm good. I got a lot done. Might go back over. Set up extra cams too. I'm excited. Talked to my commercial buddy amd he motivated me. No reason my plants can't handle this shit. I've just had massive personal shit going on. Friday is the next GOOD day but these girls might go out earlier. I'm checking the forecast. I'll sanitize the other containers in the morning and fill them. 6/5 Today is usually my watering day but shit still seems heavy so I'm going to hold off. Despite that i need to transplant. I think they're wet enough. I could do it today. Plants are looking good and weather takes a better turn Friday. It's not raining hard though. It's just sprinkle right now. I may transplant some today depending on how things go. I'll keep the diary updated. UPDATE: I went back over with the intent to just mix soil and let it acclimate. I decided to try to transplant one. I grabbed a 10th planet and started to go. I had done a bunch of manual labor already so i wasn't thinking. Of course the fucker was dry and didn't wanna come out ofcthe pot and the root ball pretty much decintigrated in my hand so I'm not sure how that will go. Tried again with the Blueberry Cheese Cake. I thought the roots just might not have been rootbound abdcthat could've been the case. After having another rough transplant I recognized I was tired, all the hard stuff is done and it's gonna keep raining until Friday. I wanted to transplant three and put them outside but with the wind and rain and the difficult transplant I let them sit under the light in the garage in indirect light. I had the doors open for a while. Should be open now but i don't want to have to go back over and shut it. Plus I'm not to keen on getting water on my light. Let's hope for a better day tomorrow. This was the only thing I had going good. Phrple punches are still behind. I've decided I'm oing to light dep ttwo of the plants in the 3 gallon pots. The two shittiest or smallest plants (probably purple punch will be light deped. WENT BACK OVER AT FIVE AND IT APPEARS EVERYTHING IS ALRIGHT. WILL HAVE CHECK IN MORNING. Vid won't upload. Tried multiple times. I'll try again tomm. 6/6 Waiting for a doctor's appointment when I should be transplanting buy whatever. Everything is looking really good. Despite me disint9grating the rootball in my hands the two transplants look great! When I get back I'm planning to get some more work done. It's still lightly raining off and on but the door is up. I'm lettingvthe soil mix acclimate a little bit too. Hard labor is mostly done. Now the intricate part. Wish me luck. Not sure if it will be today bit I'm hoping. WENT BACK OVER CAUSE IT WASNT RAINING BUT AS SOON AS I WAS CLOSE IT STARTED POURING SO THE PLANTS ARE UNDER THE ONE WORKING 150HPS AND THE AMBIENT LIGHTING FROM THE WINDOWS. TOMORROW IS TRANSPLANT DAY. I WONT HAVE A VEHICKE SO IT WILL BE TRICKY BUT IM GONNA MAKE IT HAPPEN. Opened doors at 530. Video still didn't upload 6/7 Still raining. It's off and on and a nice rain though. It's 53 degrees at noon. I transplanted two 10th planet's into 20 gallon pots. I transplanted these two a little differently. I put the bags in there final spot I side my cage andctransplanted in the misting rain. The first one came out and seemed to be heavier on the bottom and broke off. I tend to "throw" them in the hole before they collapse. With my anxiety I suck at transplanting. Plus these plants haven't filled the 3 gallon pots with roots yet but i want hem in their final homes and its time to go outside. I transplanted the other two plants indoors and let them have days in the garage with the door open and the light on then protection from the elements at night. I've been doing this for awhile. They would all be outside if the weather wasn't so shitty. This rain is supposed to stop. I'm curious as to what will be the fate of the two plantsci transplanted and left outside. I'm praying and hoping for the best. I also put my back tarp up so I have a wind break between rhe buildings. I stopped working because I didn't want to fuck anything up. Anxiety disorders suck. TOOK A BUNCH OF VIDS BUT THEY WONT UPLOAD. GOES FROM 9% TO 65% BUT ALWA7S TURNS RED "FILE FAILED TO LOAD". I'LL KEEP THE DIARY UPDATED. ESPECIALLY IF I GO BACK OVER. WAS ABLE TO LOAD A COUPLE VIDEOS. I GUESS ITS NOT AS BAD AS I THOUGHT. AT LEAST I HOPE. WENT BACK OVER AT 3 AND CHECKED THINGS OUT. THE TWO REXENT TRANSPLANTS I LEFT OUTSIDE SEEMED TO BE IMPROVING. DESPITE THE FACT I DUMPED A SHIT TON OF SOIL ALL OVER ONE OF THEM. IT SNAPPED A LITTLE BRANCH CLOSEST TO THE SURFACE. ITS STUFF I'LL REMOVE ANYWAY BUT STILL. IM REALLY CROSSING MY FINGWRS AND PRAYING THESE GIROS WILL MAKE IT. With everything i have going on tjis is my respite.IM GOING TO TRY TO SEE WHAT I CAN ACCOmplish TOMORROW. SOME OF THE PURPLE PUNCHES ARENT READY FOR TRANSPLANTING THOUGH. I ALSO ADDED TEO TARPS TO THE CAGECTO COVER THE WIND TOWER BETWEEN THE TWO BUILDINGS. THIS IS GOING TO BE A GOOD YEAR! Shut the door at seven. For some reason the two 10th planet's I transplanted (that fell all over the plant and I thought mightve broke it) look awesome lol. Despite the ridiculous pouring rain. It's going to let up soon and we are do fir some good weather. I'm trying to get everything (that isn't getting light depoed) into the cage. Four transplanted. Ten (I mean 8 more) to go.

Another 19cm vertical growth this week and she got a lot bushier. She has started flowering at last. For nutrients I'm using 15g of 20-20-20 powder mixed with 10L of water. Also added 4ml/L of both Cannazym and Terpinator. This brings it to around 1600ppm including ~300ppm for my clean water. Timelapse Sequence: Blue Dream *** Auto Jack *** (too short - out of shot) Pineapple Express CBD Compassion BLue Dream *** Auto Jack *** (too short - out of shot) Pineapple Express CBD Compassion

She's starting to get frosty!!!!!!! Pistils just turning flame orange 😍 One she goes!

Vamos familia, actualizamos la primera semana de floración de estas Frosty tooth de Seedstockers, salieron las 5 de 5, 100% ratio éxito. Aplicamos varios productos de Agrobeta, que son increíbles para aportar una buena alimentación a las plantas. Temperatura y humedad dentro de los rangos correctos dentro de la etapa de crecimiento. La tierra utilizada es al mix top crop, por cambiar. De 5 ejemplares seleccioné los 3 mejores para completar el indoor y trasplanté directamente a macetas de 7 litros, también cambien el fotoperiodo a 12/12 y aplique una poda de bajos, se ven bien sanas las plantas, tienen un buen color y progresan a muy buen ritmo por el momento, las plantas ya marcaron preflores. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨.

FBA2507 Light mix finito✔️BANG To the guy behind this strain : THANK YOU it's a one-way to the past when I was harvesting peaches in my grandpa's garden👨🏻‍🌾 Once again the quality of the buds 😳!!! In coco she s huge🚀 Same feeding + Green Sensation RoOoOaAaaADDD I've ever been down👻 Take care 💚

Cruising along, adding more fermented inputs, and WSC, will be flipping into preflower aug 1st and reducing my greenhouse light schedule from 5am to 6am and adding more red to the spectrum.

Harvest came quicker than i thought it would. Taken awhile to update as I've had a few smokes to test after a heavy harvest so sorry about that. Pretty easy one to grow overall, didn't give this grow my full 100% as had a lot going on in my personal life but back to it now! Beautiful looking girl.. She had a 14 day flush as usual!

Hello sweeties🌿❤️ This is my babe BDSM plant😂😂😀

The beginning of Week 6 of Flower and boy is she getting stinky. I have high hopes for this plant to live up to her name .

Gente !! sono arrivate alla 4° Settimana di fioritura...pensavo diventassero hermafrodita, per il forte stress subito,ma invece ho visto che ha solo fatto un paio di nanners(bananine)...sto controllando giornalmente che non c'è n'è altri e specialmente che non rilasciano polline altrimenti mi impollina tutto.e per ora non ho bisogno di semi,🤣😂💪.cmq stanno bene

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

Chopped one already. These 5 are finishing.

Day 1 of flower

So die Woche wurde ein wenig entlaubt und die haben ordentlich an Größe zugelegt. Ich Denke mal in 7-10 Tagen ca. werde ich sie in die Blüte schicken und ab da beginnt für mich persönlich der spannendste Teil🍀 Vorher werde ich nochmal düngen und vielleicht ein paar sonnen segel und zu kleine Triebe entfernen👍🍀

Fruity Pebbles autoflower growing in the Blue Planet is going huge. I am pretty sire I had 2 different phenom types growing. She is starting to bulk and has lots of bud sites to fill. Everything is going well at the moment. Thank you ILGM, and Medic Grow. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g