11/16: I had to move a bunch of seedlings into the closet and decided to just go ahead and run the lights at 24/0 for these last several days. I've got the lights to within 8 inches on all of the flowering plants and as little as 6 inches on a couple of them😈. It's been cold out, so I've been able to keep my temps nice and low. I also have a 6500k floodlight on in there to "winterize" the environment a little more. 11/18: (bugles sounds) Introducing....The Frost Queen and Princess! I harvested the last two Orange Sherbets today...Wow, just wow!😍 11/20: Today I harvested the last two Wedding Cheesecakes and all three Gorilla Cookies. Kind pressed for time, so I just did a cursory trim before taking some photos and washing them. The Gorilla Cookies are every bit as dense as the Wedding Cheesecakes. The gigantic WC has a few seeds here and there, so if I don't spot any spent nanners on her, I'm gonna assume the pollination was done by the short/bent Strawberry Pie that I deliberately left a few male flowers I spotted on. That would make for some kind of special auto hybrid right there.. Well. that's a wrap on this crop. I'll do harvest reports on each strain as soon as they've cured a little bit, but I can tell you already that I'm gonna love most of them. Aromas are incredible!

Hallo zusammen 🤙. Sie wächst sehr schön und macht keine Probleme.

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------------------------- | BLUEBERRY HARVEST | ------------------------- After two sunny days it is harvest time !! - Finally, 16 days of flush - At first thought, the trimming would be easier compared to my previous plants, but when removing more leaves, more buds appearing too hahaha so it took like 4 hours or more..It is not the most accuurate trimming but i had not much time that day, i removed all the leaves and branches after weighting it all.. Like i said on the last post, im harvesting having in mind my bud preferences (the later the better), weather, humidity and sunlight, so the first of the cloudy/rainy days she was chopped in the moring, attending the principle of thc regeneration at night.. Got to say that, i wasnt expecting such production (8 days until it was well dried), this plant started as the season saver because the Cheese started to flower in march, revegged etc.. and i have been rewarded with this BB, a true genetic portent !! If i could, i would keep her as mother forever ^^ The quality is awesome, she looks more like a WW talking about that coat of thc.. Flavour without proper cure, is deep earthy at first, transitioning to a fruity, sweet one, it is a pleasure to smoke, delicious sweet aftertaste.. Maybe, this is the last year with this awesome climate, because im planning to leave Spain.. Thank you for follow and check !!! I hope you liked the diary :) Song of the week : Radiohead - Codex Good Vibes GrowDiaries !! ---------------------------------------------------------------------------------------------------------------------------------------------- 000000000000

Was another easy week - lots of healthy growth. Simply watered when dry, did some daily LST and leaf tucking. The green crack is definitely the fastest growing out of all the plants, and the one runt LSD25 is a bit smaller but still chugging along.

Watermelon auto is hurting this week. The neem oil spray is always so harsh on my plants. I am trying a different a way of spraying them, spray, wait a half hour, and rinse off. But leave on the under side of leaves. Since they like to hide there. Hopefully it will help. I have not seen new ones on this plant. They were on another. It has been a horrible year for issues in my grows. Thank you RQS, Gen1:1, and Medic grow.. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

2/26: Harvested both Black Jacks and both Gorilla Girls tonight. They got a rough live trim, then chopped, washed, and hung to drip dry. Big, heavy, frosty buds on all 4 of them!👍 3/2: Still drying in the closet...amazing aromas!

Requiere mantener bastante baja la humedad en las últimas semanas ya que lo grueso de las colas las hace propensas a la botrytis, ojo ahí. La planta en si es resistente pero es el grosor de sus flores, lo denso de las colas lo que te obliga a tener muy controlada la humedad en etapas avanzadas, creo que es más para tirarla programando la flora para el verano

Forbidden Runtz is coming down soon since ambers have started showing. I am just waiting for some groce bags to show up to cure in. Sour Stomper has surprised me will a burst of growth. She's still atleast a week over more.

~ FastBuds PAPAYA SHERBET Photoperiod~ Well here we go on another journey together through 'Canna-land' and this one's kinda special... forget that, it's VERY special because this is FastBuds newest surprise for the community, a FEMINIZED PHOTOPERIOD STRAIN!🤯 I was fortunate enough to score these seeds, of which only 1000 were available and can't wait to see what this strain can do! This Papaya Sherbet, according to FastBuds, is a hybrid with a 9-10 weeks flowering period. For a more detailed and accurate description of this strain the following from FastBuds says it best: "Combining the massive stature of Papaya (Oni Selection) with the strength and resilience of one of our best keeper cuts (Sunset Sherbet), this strain develops into a big, expansive bush adorned with numerous bud sites that later transform into a generous harvest of medium-sized buds. Papaya Sherbet flowers deliver a signature flavor of premium cannabis with subtle citrus undertones that emerge upon inhaling. During growth, her aroma makes for a sweet yet pleasantly bitter fragrance, giving you an idea of what the smoke will taste like. Notably stress-resistant, Papaya Sherbet is a great choice for growers working in challenging environments. She is very forgiving and rebounds quickly from any adversity, allowing growers the freedom to experiment with confidence that she will take everything like a champion she is. This strain embodies resilience, flavor, and abundant yields in every grow cycle." Sounds like an epic strain and I personally cannot wait for this lady to strut her stuff!😍 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ The Setup: This is going to be an outdoor grow, but I have started the Papaya Sherbet photoperiod indoors as our weather is still a bit too chilly to put a newly sprouted seedling outside (nighttime temp's dipping regularly into the 40's℉). The plan is simple... let her grow inside under a 19/5 light schedule until the nighttime temperatures are in the mid 50's℉, which shouldn't be long. After which, she'll be moved outside and transplanted into the soil which I have already setup and inoculated with beneficial microbes from BioTabs and slow release dry amendments from Gaia Green. Once she's established herself outside she'll be given periodic top dressings of Gaia Green 4-4-4 and 2-8-4 along with worm castings and Compost Tea's. Her grow area is approx. 5'x5' and I have posts and a trellis net set up already for when she gets bigger to aid in training her. Let the fun begin!🤪💚 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 5/31- Week 4 from seed and the FastBuds Papaya Sherbet photoperiod is doing a lot better now that she's going through a growth spurt! The leaf chewing has mostly subsided now since I have been spraying Neem Oil on her every 2-3 days. She's also beginning to increase in height about an inch per day now and has great color and vigor. 6/2- Yesterday I top dressed the Papaya Sherbet with 2 cups of Gaia Green 4-4-4, 1 cup of Down to Earth Bio Fish along with 1/2 gallon of worm castings. After top dressing and working the amendments into the soil by hand, I watered it in via garden hose with straight well water. 6/4- It rained hard yesterday, so I skipped watering the FastBuds Papaya Sherbet photo today as the ground was still pretty wet. 6/6- The last two days have been sunny and warm so I watered the Papaya Sherbet photo with straight well water from the garden hose today. Another week gone by for the FastBuds Papaya Sherbet photoperiod and I'm looking forward to next week, as well as the weeks that will follow to see how this lady progresses! Thank you for checking out my diary, your positive comments and support make it all worthwhile! 💚Growers Love!💚😎🙏

Transplanted early this week. This girl showed her pistols under 24hr light so I'm guessing the genetics for her are strong. I plan to let her go and take a few clones. She also got switched to 18/6 light. She is absolutely Killin it

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Solid week!! Fed her bloom nutrients on day 50. Responding nicely. I pulled down the main 8 to allow for better light and airflow throughout. Once I did that she really started to fill in again. This also seems to help stop the stretch, or maybe it was just timing and stretch was coming to an end anyways. Either way, glad it did as I am am running out of room to raise the lights and have no more options for setup. Bud sites are all starting to fill in good and fatten up a tiny bit. Looking forward to seeing how these shape up!!!

Everything is ok I transplant the sluricane to the greenhouse with the sleepy Joe :)

Week 18 Middle August : weather is changing. Return of heat and humidity: 28 degrees, a little bit rain. A heat wave is coming but near the ocean the maximum will be around 32 degrees. It’s good to grow in greenhouse during this period : -ventilation control: I adapted the rythme: for now it’s around 18h/24h intermittently -pests and diseases control: insect’s traps natural and artificial, less heat -humidity control: no rain problem Daylight 14h: 7h/21h Flowering time 😁. Healthy plant, beautiful green color, just a few yellow leaves on the base. More watering: 1,5L every 60 hours Same cocktail: Calmag (0,5ml/L)+ FloraGro (2ml/L), FloraMicro (2ml/L), FloraBloom (1ml/L) + Root booster + Cannazym Feeding soil with guano bat (NPK 3/6/10) for a slow dispersion + dried horn and blood (NOK 13/0/0) for nitrogen add. Protecting plant: sprayed Neem Oil, added diatomaceous earth on soil. I will make a defoliation soon.

Starting to take shape and smell😂 was able to take a fastbuds down this week to help the ramp into harvest. 😂😂 gréât strains - spring is here - on Twitter now @greenleopard420 - all good. Variation if feeding in the tent. Those little fleas still there , nematodes didn’t work so disappointed there.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

2024-12-25 to 2024-12-31 With less than 2 weeks remaining to this grow, I started the flush - straight water w/ CALMAG for one more watering, then straight water for the last 10 days. With the high humidity from all the rain we have had, as well as higher than average winter temps, it has been difficult to find that sweet spot for Vapor Pressure Deficit (VPD). However, we are predicted to have cold and dry weather starting New Years Eve and lasting for a couple of weeks. This will allow me to control the lung room environmental aspects to try to hit that VPD sweet spot for the last couple of weeks of flower. 12-26 Day 47/101 Flushed w/ water and CALMAG - 1.5 gal each 12-30 Day 51/105 Trichomes are mostly milky, with less than 5% amber 12-31 Day 52/106 .5 gal water + CALMAG each. Leaves are starting to show nutrient deficiency from the flush. Water overflow in trays is reddish in color, indicating a large amount of nutrients still in the soil.