2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Well into flower now,no issues so not much to report.day 15 of bloom 🤞🍁

✌️🎃 Thank you for checking my cultivation. 🌰 #1 and #2 (31/05) #3 (02/06) 💪 #1 survived 🔥 #2 died burned ⚰️ #3 didn't complete germination ☘️ I wasn't very lucky with the wedding cake. I don't think they like much nutrient - Buffering on coconut EC 0.6

Today They have pop in my new room is completely ready for a New girls season I am going to use a rosin press for a large quantity off all the flowers That we produce And I will be reviewing that brand ones it arrive

su primera semana fotoperiodo 12-12 y mis bellas se han adaptado a todos sus cambios, una vez mis bellas indique que comenzo su floracionn hare cambio de nutrientes.

11March (VegDay24) Start of week 4 of Veg and with some minor defoliation on both girls, going to stabilize PH at 5.8/6.0 in the coming days. the girls are having 1lt each every 24h RR1 looks like she could do some more so I will start doing 1.2lt for her and keep RR2 at 1lt. 12March (vegDay25) Din some more LST on RR1 and took off 2 big fan leaves also RR2 have some more LST and small defoliation. 13March (vegDay26) increased watering for RR1 to1.5lt also increase nuts and stabilized PH at 6. 14March (vegday27) increased amount of water for both girls today and got a decent amount of runoff! slightly increased a bit more the nuts for one last time before the addition of bloom nuts end of this week. both girls continue to stretch and look healthy. RR1 50cm+3cm RR2 41cm+7cm 15March (Vegday28) as of today, bud candy of AN is part of the team as from now PH is steady and plats look amazing! 17March start of pre-flowering both girls have heavy signs of buds, will mix both grow and bloom nut as from this feed.

Hey guys, I'm in bud heaven right now. Everything is maturing, lots of dense crystal covered buds forming everywhere. The Cinderella 99 is the slowest to flower and is still stretching. Do si dos is in full flower. Beautiful tight resin covered nugs. Going to be a nice early finisher, finishing mid-late September. I am keeping an eye open for mold and mildew. Treating the plants weekly and closely monitoring. The slower flowering plants won't be ready untill mid October. Happy with the results and mother nature so far. Until next week farmers!

Watered today everything is looking good! All strains developing distinct scents now. Fat Banana has a Sprite like candy smell. The Blueberry Swirl has more of a lemon zest smell, was hoping for blueberry scent but hopefully it will come later. Tropical Cooler has the smell of a rotten fruit salad. Lemon Haze x Garypayton has more of a berry scent. Defoliated Blueberry Swirl, their were too many popcorn bud sites so I cut the branches off.

I sowed cantaloupe bag seeds directly to flower box drip irrigation.I transplanted my white widow seedling to flower box. So the new line up this run is cantaloupe, blueberry, Girl Scout cookies and white widow. Cantaloupe seeds sprouted and i will keep the most vigorous grower.bb is throwing pistils.GSC is producing first set of true leaves. At the end of week 2 cantaloupe has first leaves so does white widow. My Gsc has first set of true leaves and my blueberry is putting out pistils and trichs.Things are going well

Time for flower. Cleaned my flowering tent & equipment & transferred the girls from veg tent. Developed really nice structure on these plants. Use the promo code GROWEED for a 15% discount on any Fast Buds purchase!

08/26/2019 Week 4 of flowering gonna give 5-10-5 last dose and she what she does, hope it helps put on some weight cause she is looking a little bit smaller than I hoped for, but she smelling like sweet candied OG.

Día 37 (08/07) Ajustes de LST en todas las plantas! 🚀 Día 38 (09/07) Ajustes de LST en todas las plantas. Las ramas inferiores están creciendo como un cohete! 🚀😍 Riego con 250 ml H2O pH 6,5 Día 39 (10/07) Las plantas se muestran sedientas! Creo que el cepellón está lleno de raíces y piden el trasplante! El crecimiento no se ha visto afectado de momento Riego con 500 ml H20 pH 6,5 Día 40 (11/07) Paso a regar con 500 ml / día, ya que hace mucho calor (30 ºC) y efectivamente las plantas ya han copado la maceta de raíces Las ramas de nodo inferior se han quedado enanas tras el entrenamiento, de modo que las elimino y aplico canela en polvo en los cortes Riego con 500 ml H20 pH 6,5 Día 41 (12/07) Riego con 500 ml H20 pH 6,5 Clones! Mi experimento vuelve a demostrar que NO necesitas complejos productos de clonación ni sistemas para obtener clones! Hace 15 días puse 2 clones con el tallo sumergido en miel durante 5 minutos en una maceta de 400 ml con PRO-MIX HP BACILLUS + MYCORRHIZAE He mantenido la humedad alta con una cúpula de humedad casera hecha con una botella de agua PET de 5 litros cortada por la mitad y pulverizando a diario. Han estado los 15 días en mi terraza con luz indirecta, y temperaturas que rondan los 30 - 32ºC durante las horas centrales del día Hoy los he trasplantado a una maceta de 1 litro porque ya habían formado raíces y empezaban a formar nuevas hojas! 🐥🐥🚀🐥🐥 Día 42 (13/07) Riego con 500 ml H20 pH 6,5 Día 43 (14/07) Trasplante a maceta definitiva de 21 litros de ROOTPLUS Pot de GSKOREA GLOBAL! Estas macetas son una maravilla! Proceso de trasplante: Se prepara con 17,85 Litros (85%) de sustrato PRO-MIX HP BACILLUS+MYCORRHIZAE + 1,05 Litros de Humus de Lombriz (5%) + 2,01 Litros de Insect Frass (10%) + 210 gramos de Earth Vibes Super Soil (10 g/L substrato) Se llena la maceta de sustrato con las manos (limpias) y rompiendo los trozos más gruesos, para que el sustrato esté aireado y esponjoso, sin presionar Se coloca una maceta vacía de 6,5L para que quede la forma perfecta de la maceta donde están actualmente Se espolvorea la parte proporcional de la probeta de microorganismos sobre el agujero de trasplante Se saca la planta de su maceta actual (bonitas raíces 😍) y se coloca en la maceta final Se riega muy lentamente hasta percolación profunda con H2O EC 0,5 pH 6,5 Una vez asentada, complemento con un riego de 500 ml con 25 ml/L de Humus de Lombriz Liquido Se coloca mulch (acolchado) de paja para evitar traspiración excesiva y cuidar a los microorganismos del suelo A ver como reacciona al trasplante! Aplicación foliar Kelp hidrolizado de Lurpe Solutions a 0.25 ml/l Realizo ajustes de LST aprovechando el trasplante a la nueva maceta. De momento tienen una canopia muy bien formada! 💦Nutrients by Lurpe Solutions - www.lurpenaturalsolutions.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

We are on week two of flowering and the ladies are loving it .. we defoliated and pulled some branches around increasing air movement and light penetration.. The cheese has been a little picky .. lighter on the nutes and doesn't like training very well..The team @seedsmen seeds have bomb genetics and I can't wait to stArt my next seedsman diary sooo good ..keep an eye out for week three ..

now im start to using many kind of sugar till harvest, beer-fpj-molasses-coconut water...

Hallo to You all my familly and usual visitors. Thank you for stopping by and all the likes and comments....You make my day! I gave the Blue Cheese a trim on 5th december and gave her four days of darkness, the chop was performed on december 9th I took a lot of photographs and videos commemorating these events for you to enjoy :) I think she came out quite decent especially considering that she had some monster/deformed leaves till the 4th week of veg. also looking back I should have moved her to the bigger place in that time...she would be far more symmetrical ;) It was a joy to work with Mars Hydro and their equipment in this grow. A big thanks to the Mars Hydro team for this opportunity, you truly provide first class grow kits and lamps. That's all i have for you till the drying & curing process is over I will update then. I want to thank you all for accompanying me in this journey, all the likes and comments...You growmies make me feel here at GD at home thank you 🙌👊😇

Heya👍🤙👍🌱 Apricot stopped drinking. Check out my Facebook profile: https://www.facebook.com/share/v/H6PnoahTHrEzm63U/?mibextid=oFDknk Big BIG BIG thank you to Sebastien, Heather from Fastbuds420. You guys are the best. Can't wait for the next live. Even Bigger shout out to Hydroponic.co.za. My local Hydro Shop and Sponsor. Thank you Sir. 👍🤙👍🌱

He dejado la semilla 24h en el vaso con agua de osmosis hasta que se abrió, luego la pase a un taper con discos de algodon humedecidos.

Apple Fritter is doing well. Has one mutated Cola with tons of pistils. Pretty neat looking. I just did a solution change. She is in a New Level Hydro bucket, under a Spider Farmer SE5000 light. Thank you Spider Farmer, New Level Hydro, and Amsterdam Marijuana Seeds. 💪❄️🌱 Http://amsterdammarijuanaseeds.com Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g SE5000 https://amzn.to/3qFpAML Spider Farmer Official Website Links: US&Worldwide: https://www.spider-farmer.com UK: https://spiderfarmer.co.uk CA: https://spiderfarmer.ca EU: https://spiderfarmer.eu AU: https://spiderfarmer.com.au Coupon Code: saveurcash Www.newlevelhydro.com Www.hygrozyme.com

03/27/2020 start of week #4 flower this girl is in full flower mode can't wait for next week. Did final defoiliating today. All is looking well should start filling out soon. Thank you for checking out my diary🙏 Going to raise lite to see if she will stretch more, pistils popping out like crazy can't wait for Calyxs to swell