6/27-8/3: In my time away, Ms. Cookies did great. She was quickly drinking down her water through the self watering base and she ended up eating all of her nutes, leaving some signs of these deficiencies when I got home. In addition to that, the soil pH was a little high at 7. So with these 2 deficiencies in mind, I flushed her with plain 6.0 pH water to bring the soil pH down, and I also amended the soil with some All Purpose 4-4-4 and Power Bloom 2-8-4. I also did some light defoliation of some larger fan leaves Overall, the plant is looking great. Had a minor setback with the deficiencies but it was honestly expected since I wasn't home to watch her closer for 2 straight weeks.

9/8/2024 Plant B showing signs of female with hairs coming from the pre-flower formations. Looks to be stretching and starting to push into flower as well. Plant A is growing insanely well! Continuing to defoliate daily by removing larger fan leaves here and there to continue to open up the canopies on both plants. No complete water change yet since a few weeks ago. I have continued to add fresh nutrient water with an occasional pH-adjusted gallon of filtered water + cal mag. 9/10/2024 Both plants and stretching nicely and developing great canopies! Plant A is an absolute monster, plant B is small and compact but has really good node spacing already. I am expecting some nice flower development over the next few weeks! Topping off the reservoir with about 1 - 1.5 gallons per day of nutrient water at this point. Continuing to prune a good handful of leaves and smaller growth from the plants to open up the canopy leading up to day 21 final trim. Trying to stay ahead of a huge stress event for the plants. 9/12/2024 Continuing to defoliate handful of leaves and prune smaller undergrowth. Cutting only 2-4 stems per day to avoid over stressing. 9/13/2024 Raised lights over plant A a few inches as it continues to stretch. Also trying to keep the lights low enough for plant B without burning the side branches of plant A. 9/14/2024 All week I have been adding nutrient water to the system. No water change or replacement. However today, I decided to introduce just pH-balanced water to drop the nitrogen concentration in the system as we transition into week 3 flower. Highest PPM throughout the week was around 900, average 800, low in the PM after pH water 700. Had a minor flow issue with a root plug in one of the drain lines. Blew them out and flow returned. Humidity in the tent has been slightly higher than ideal this week, although VPD has been around 1 - 1.4 with the lights on. Likely due to the volume of plant matter in the tent. Doing my best to keep up with defoliation and trimming. Day 21 flower will be much needed!

Que grande es esta variedad, muy contento con El Progreso vegetativo de estos ejemplares. Iremos viendo estas semanas cómo florecen ya que cambiamos el fotoperiodo, la cosa os Aseguro que pinta muy bien.

ya ha empezado con el estiron

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

what a journey, such a beautiful result i should retire now how can it become any better. thx everyone watching by. have a nice day and an even better grow

Now she puts the weight on

This was the easiest to grow. Shook off light stress and hard water to produce more yield than any of the others. Will be growing again. Dense, golf ball size nuggets.

Bonjour à tous Comme vous pouvez le voir , RAS pour cette semaine , seulement deux gros arrosage , cela fais 4 jours qu'il fait plus ou moins 50 degrés dans la serre mais avec un bon sceau d'eau dans un coin , cela ne leurs poses aucun problème !!! Je suis toujours en près floraisons.... Pour l'instant c'est vraiment léger le passage en floraison Hormis la "Black Domina 0" et oui, le retour... Elle se porte très bien mais étant isolé ailleurs je ne peux pas la prendre en photo entière... Niveau engrais , Peau de banane à tout vas et Marc de café , je tiens à signaler qu'en cette fin de semaine dernière un important arrosage avait était fais avec du fumier de vache , cet engrais est naturel , gratuit et très riche en nutriment !!!! Je pense arrêter de fournir de l'azote pour la semaine prochaine afin de forcé "un peu" le passage en floraison Bon week-end !!!!!!

Sorprendiendo cada día más OGKush auto

I’ ve been really really excited in growing this 🍊. I really loved it to do ti in grow diaries. Community helped me and I hope I’ll be doing always better. I think next time I’ll do a foto to play a little bit more with structure of the plant. Buds smells like orange and cotton, chocolate pinus. Can’t wait to try it. I’ll update in some days when I’ll have some nice buds a also le to sm oke🕹️ Thanks to all the people the followed me and that helped me with important suggestions

This was amazing so for. Thanks for all the growers love and support🤙👍🤙🌱

Week 4 Broad Mite still making victim. On this week I watered she with water + diatomaceous earth Still waiting for better days, I hope hv succes 😐 Apparently the diatomaceous earth is working against broadmite 😅

Mit meiner Blueberry Zkittelz bin ich wirklich sehr zufrieden. SIe hat einen Strech von 20 cm in einer Woche hingelegt und entwickelt wunderschöne Buds😍 An Tag 51 hatten meine Ladys noch einmal Freigang da ich mein Gewächshaus etwas umgebaut habe. Ich war am WE unterwegs, daher gibt es da keine Bilder

Self composted Supersoil Grow. End of week 4 flower. Everything is on track. Spring Water every 2-3 days and recharge (every two weeks).

Langsam kommen die Blüten✌️✨️🌱