Some good defoliation down low. And, a whole lot of scrogging this week. Not much to report. Time to sit and wait for the magic to happen now.

12-16-2024 - Plants continue to do well. Found some rust spots on several of the leaves for the Granite Runtz...could be a bit of over-feeding as I top dressed recently despite being on Grow Dots...not too worried about it though. These guys are drinking like crazy so there is plenty of time between feedings where they're getting a little flush action. I'm going to guess that we're about 3-5 weeks out from chopping these down and I'm impressed with their size so far. Now I'm really just waiting for the buds to continue developing and see what kind of size we can put on these gals. This grow has gone unbelievably well and I feel like I have the tent dialed in pretty good. More to come! 12-17-2024 - Well this morning the girls got a bath in Recharge and Sweet Berry. They were very thirsty! The nuggs on these plants are getting massive and I'm expecting to see branches start to struggle to carry the load. At this point I'd say they're probably 3-4 weeks out from chop...but that is a very loose estimate. I'll keep a close eye on them as we go. Having so much fun watching these girls grow huge. CANNOT wait to weigh these things dry. I really think I'm gonna be close to a pound with this tent. 12-19-2024 - Got some nutrient burn going on with the Granite Runtz...just the top leaves and other than some orange speckles on the leaves the flowers look good and overall the plant looks healthy. The GR's have by far the thickest/most dense flowers of the three. The Frosted Cherry O's look very balanced and is the most attractive plant in the tent. The Mystery Seed, which is the only one I topped, is just growing in every direction and has tons of flowers but is clearly behind the rest in terms of maturity. I'd say in a few days to a week it'll be much thicker. Today I watered each plant with 3 Liters of dechlorinated tap water PH'd to 6.5. I'll take a look at them on Saturday morning and see how dry they are. Basically they're drinking a half gallon of water per day. Probably 4 weeks out from harvest is my best guess right now.

All good this week too. The strawberry lemonade turning purple!:)) gg#4 it’s a hermy… the other plants are good! White widow xxl it’s the first to harvest…In 3 weeks…. Fxxk

Spray evolution 2.0 once a week biosys instant microbe tes once a week

Esta semana ya estamos casi terminando, al menos nuestra auto gelato estará lista para el corte posiblemente en unos 10 días mas. Si bien no engordo mucho por el ciclo 12/12 (es lo que esperábamos y el por que no sale nada rentable poner autos 12/12) aun así promete dar una producción interesante y bastante fuerte por la cantidad de cristales que esta mostrando. Respecto a nuestra sublimator y Amnesia Molotov, están prácticamente bañadas en tricomas, con un olor dulce muy intenso y que al parecer tienen mucho mas por engordar y crecer, por lo que todo indica que tendremos una producción bien generosa de ellas. ya la próxima semana posiblemente estemos compartiendo la cosecha de nuestra automática y mostraremos que tal fue el resultado de esta niña sin darle todas las horas de luz que necesitábamos (insisto, no buscabamos el mejor resultado, buscabamos mostrar por que es mala idea poner automáticas y feminizadas juntas al mismo tiempo que aun que parezca loco, es de las preguntas mas recurrentes que me llegan).

Day 30 Feb. 18th- I feel like everything is going great -all plants are now in final 7 gallon containers..have used a lot of soil! -I have been tying the plants down as much as possible -March 2nd will be 42 days and I think that is when I will switch to 12/12 -Day 35 Feb. 24th- I need trellis netting soon I retie branches constantly for inner growth HULKBERRY and GREEN GELATO are growing similar...LSD and ROYAL GORILLA are growing similar! -Very interesting!! * I have color coated the plants like I should have a couple weeks ago -GREEN GELATO has all green pipe cleaners now...HULKBERRY has all yellow pipe cleaners. very long branches and small inner growth LSD is in all purple pipe cleaners. tying it down has done wonders! it has awesome inner growth! Now the ROYAL GORILLA in all orange pipe cleaners! This plant is a pain in the ass lol. The inner growth and spacing is insanely close!!! so many shoots all tightly together. I have tied down anything I can. Unsure how it will work in budding next week

She's surprisingly short compared to Selena (Super Auto Lemon Haze) but her buds are turning out to be nice, purple, and covered in trichomes so far. Had to cut down the lights to 12-12 cause one of the other plants turned out to be a photoperiod. Sucks but it definitely saves me the electricity bill. As usual, looking forward to next week!

SO UPDATE GANG!!!! Everything is going well so far, i stocked up on water for the girls nutrients are doing well but im going to upgrade them to the FOOP line of OMRI nutes other then that will keep everyone updated so far thanks!

Couple plants at doffeent weeks here's a quick tour of the tent

Everything looking good...

Week 9 of Flower - Beautiful colours and a crazy smell

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Sunday, May 29, 2022 Plant #3 is stretching away by a foot over Plant #2. Both Plants looking well but displaying different leaf behaviour (particularly of the upper growth). When well watered, in full sun, Plant # 2 has perky, praying leaves that go down somewhat at night. In the same conditions, Plant #3 has droopy leaves that get even more tucked in at night. It was only from feeling the strong turgidity of the Plant #3 leaves when under good water and comparing them to Plant #2 that I accepted that it was ok and that's just how it wears its' clothes. The red and white pistils are filling out from top to bottom on all stems on Plant #2. Even though it's not as tall as its' neighbour, the six main stems on plant #2 seem proportional to, hopefully, support nice thick colas. Plant #3 is furring out much slower and though I am happy for the height, I am concerned the stems aren't thick enough to support much weight.

Week 1 - 01/01 to 01/08 2021 HUGE THANKS TO @MARSHYDROLED and @VIPARSPECTRALED! New surge protectors/heavy duty timers/the good cords. The Red Poison by @Sweet_Seeds is right on track with the Dark Devil and Purple Lemonade from my other diaries. The LSD is a little behind but the genetics seems to keep it small if you get the wrong pheno. Will give it a week or so for it to really settle in with a root base before cleaning out my 5gal RootPouch pots from the last grow and getting them transferred into their final homes. I have all my seedlings under the P1500 from Vipar as my veg light for the first few weeks to a month. It's SO nice to not have a blurple anymore, the light is way better in my room and on the eyes. The new diodes had every single seedling grow more in 6 hours than it did in the first 7 days under the blurp LED. The Red Poison perked right up and threw another set of leaves in the first 6 hours under the new light just like the Dark Devil and Purple Lemonade. I noticed a bit of spotting on the Dark Devil in my other diary so I added some Hydrogen Peroxide to the spray bottle and it cleared up. All four have been getting constant spritzing from the spray bottle to keep humidity up. Excited to see how big i can get the autos under the new lights, will switch over from the Vipar to the Mars by end of week 2/mid week 3 once I transfer them and give them a day or so settle in. Thanks for stopping in! Throw down a like and/or follow so i can return the favour 🙌 -- Check out my Diary of the Year 2020 entry 🙏 https://growdiaries.com/diaries/54258-fastbuds-original-auto-sour-diesel-grow-journal-by-glazedgrow IG: @GlazedGrow CannaBuzz: @GlazedGrow

Week 11 Light cycle=12/12 Light Power=130w Extractor controller settings High temp= 24c Low temp= c Temp step=0c High Rh= 40% Low Rh= % Rh step=0% Speed max=10 Speed min=1 Smart controller settings (during lights on). Lights on=10.01-21.59 Radiator on= below 22.0c Radiator off= above 23.0c Dehumidifier on= not in use Dehumidifier off= not in use Smart controller settings (during lights off). Lights off=22.00-10.00 Radiator on= below 18c Radiator off= above 19c Dehumidifier on= not in use Dehumidifier off= not in use Seperate feeding program. #1 and #2 go onto dragon force. #3 and #4 stay on schedule. Thur 7/3/24 #2 (Day 71)(Day 39 flower) 📋 Fri 8/3/24 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 Method= Manual Feed=ripen nutes. Dragon Force=4.0ml/L Sumo Boost=1.0ml/L Easy Ph up=0.1ml/L Ec=1.45 PH=6.2/6.0 Total volume made=5L(L) Total volume left=0L Total volume used=5L Volume per plant=2.5L Runoff. Total runoff=1.5L Ec=1.9 PH=6.2/ 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 #2 (Day 72)(Day 40 flower) 📋 Ripen nutes. Sat 9/3/24 #2 (Day 73)(Day 41 flower) 📋 Sun 10/3/34 #2 (Day 74)(Day 42 flower) 📋 Lowered light power from 130w to 120w Small defoliation. Mon 11/3/24 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 Method= automatic Feed=water Neutralise=0.1ml/L Easy Ph down=0.008ml/L (3 drops total). Ec=0.2 PH=6.0/6.4 Time start=12.00pm Finish time=13.45pm (11×5 minute runs with 5 minute gaps) Total flow rate=190ml/min Flow rate per plant=47ml/min. Total volume made=13L Total volume left=3L Total volume used=10L Volume per plant=2.5L (Est) Runoff. Total runoff=2.5L Ec=2.25 PH=6.1/ 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 #2 (Day 75)(Day 43 flower) 📋 Tue 12/3/24 #2 (Day 76)(Day 44 flower) 📋 Wed 13/3/24 #2 (Day 77)(Day 45 flower) 📋 Lowered light power from 120w to 100w H=100cm D=13cm DLI=50 This is going to finish 1st, the development has been really rapid, even lower buds look good already. Because of the light stress she has been getting a few higher leaves have suffered, I may remove a few to get a bit more light penetration to some lower foliage. I have been real happy with this one all grow. Back soon. Take it easy.