9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

She fully recovered from the heavy defoliation now i hope she puts on the weight now August 13th: plant got flushed and right after fed ripen untill 300ppm runoff, the hairs start turn brown so in 10 days she will be ready. It has sativa shaped buds so i dont have to wait for swelling anymore

Im doing a bit of defoliation, moreover the leaves that covering the buds, i cut some small branches on the bot of the plants, to keep energy for the distal parts of the branches. Every 2 day approximately, to no stress a lot the plants, I pinch the apex of MD1, MD2 and BB1, that my secondary branches go up, until the height of the apex central. Nutriments : 2,0 mL/L of rhizotonic, 2,5 mL/L of cannazym every watering. 2,0 mL/L of terra vega every 2 or 3 watering in case of they need (N) until the final of the strecht (first 2 weeks of bloom). I will put 5L every watering (pots of 18L) and I will analysed the drain of every plants to check pH and the fertilizer amount. Im really proud of theses plants, they are strong, resistant (the trunks are huuuge, moreover the Moby dick from Dinafem) and so healthy !! The MD 3 has reacted really really good with the topping ! Im impressed and quite sure do topping next time ;) I hope they will give me the best of themselves !!!! Lets wait the final of the strecht 😉

What’s growing on everybody?!?! Exciting week in my Banana punch grow! The plants are really growing quickly now. Got the light cycle switched over to 12/12, got my first layer of trellis screen put up so I can start weaving branches through. I also started the bloom feed today, which just substitutes the grow for the bloom as far as my botanicare line goes, I like to use a small about of MOAB to really get my plants to start flowering. I’ll have more boosters coming on in the later weeks of flower. I will continue with my IPM regiment of spraying Athena IPM twice a week until the third week of flowering, which at that point I will cut off all sprays. I plan on running this strain until my planned harvest date which is 3/13-3/20. I vegged until my plants were telling me they were ready to go to bloom, I let the shape and hardiness of my plants dictate when to flip them, I wasn’t rushing them. I’m excited to see how everything looks in the coming weeks! Thanks for looking, As always, Happy Growing everyone!

Day 3 - not much training needed.. SO3 is a giant. Smaller buds than the other 2. Lollipopping needed

Development is good, plant smells very nice Not too skunky, i like it ;) Plant is loving the nutrients...

Week 1 complete and on to week 2. Some sort of issue has occurred. There is a some dark green on the vains of the leaves. Not sure what it could be. Otherwise everything is going well.

🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀 Welcome to week 12, dear growmies!! 😘 I'm back and everyone survived haha! 😎 From now on only boosters. 💧 Thanks for showing up and supporting me and the girls, bless you, growmies!! 😘 ❤️ Shouts go out to my sponsors @GreenBuzzNutrients, thanks so much for your support!! 💚 💚 💚 If anyone would like to try their amazing organic products, you can find a generous discount code of 25% in my weekly comments! 😜 🙏 If you want a recommendation which product to get first.. BIGFRUITS!! 😍 I love the tasty terps with this one!! https://greenbuzznutrients.com/ Thanks also of course @Kannabia, for the beautiful genetics!! 🧡🧡🧡 Pouring all my love into this grow, thank you for joining in, growmies!! 💚 💚 💚 😘 ❤️ ❤️ ❤️ Wishing you all the best for your beautiful gardens!🌱 🙏 Grower love!! 💚 _________________________________________________________ Strain Info: https://www.kannabia.com/en/feminized-cannabis-seeds/break-up-cake We need to talk You’ve got to celebrate everything in this life, and that includes love and heartbreak. Since you’re all familiar with Wedding Cake feminised marijuana seeds, we’ve put a Break-up Cake in the oven at the Kannabia Seed Company – because things don’t always go well, and we sometimes need a friendly seed to lean on. Break-Up Cake has a base of Girl Scout Cookies, one of North America’s best-known strains, which is earthy with a very potent indica effect. And we top it off with a delicious Cherry Pie which, in addition to giving it an elegant dark attire, adds a silky, deeply sweet flavour of cherries and berries. Look no further – it’s the perfect cake. This seed will be celebrated for its ease of cultivation and abundant production. It’s one of the best investments you’ll make this year. But if it’s going to be memorable for one thing, it’s for its sweet complex flavour that’s full of contrasts. If we have to break up, let’s do it sweetly and amicably, right? How to: Break-Up Cake Break-Up Cake is an “all-terrain” marijuana seed, which will adapt to the place you allocate to it. An ode to independence, it’s a plant with only the most basic needs that knows how to grow by itself. It’s a pale branching plant with tremendous dark green leaves, purple tips, and an icing sugar coating of trichomes. Its immense buds are some of the stickiest ones we’ve found in our seed bank recently. Indoors, it needs 60 days of flowering to give no less than 600 grams per square metre. Use a Screen of Green method without hesitation, or plant it using hydroponics if you want to obtain the full benefit of its potency. Outdoors, it reaches two metres in height with a harvest of 800 grams per plant, which is reason enough to give it a go. Like a good break-up cake, this plant doesn’t need to live with a partner, and knows how to live well in a Guerrilla plot. Between 25th and 10th October is its moment. Taste and effect of Break-up Cake Very relaxing but not causing lethargy, it’s a good indica-dominant plant. Its effect is mellow (we’re too old for childish games). It calms the consumer but it also gives rise to creative moments. Head on up to the top floor, later to make your way, little by little, down to the kitchen later (where you might want to give in to temptation if you need to stimulate your hunger). The flavour is full of nuances. It will remind you of a vanilla sponge or cheese cake: you’ll notice a cherry topping, something sweet that you can’t quite distinguish, and an earthy undertone on a biscuit base… You’ve got to sample it to know it. _____________________________________________________________________ SETUP: 80x80x180 cm Zelsius 240W Full Spectrum LED IR UV dimmable DW240H-A6-HS Heatsink color red LED Chips: 512pcs SAMSUNG LM301H + 24pcs Osram 660nm + 8pcs Osram IR 730nm + 8pcs UV 385nm Color mix: 2700K + 4000K 2,8umol/J Driver HLG-240H-C2100B Coverage: veg 5x3ft / flower 4x2ft Product size: 628x205x68mm Green Buzz Nutrients Shouts go out to my sponsors @GreenBuzzNutrients, thanks so much for your support! 💚 💚 💚 If anyone would like to try their amazing organic products, use code GD42025 for generous 25% discount (for orders of minimum 75€) ✨ https://greenbuzznutrients.com/ Biobizz Lightmix custom exhaust fan 320/270cm³/h Carbon Active Granulate 240cm³/h tab water pH 8 - EC 0,25 with Calmag to 0,5 Advanced Hydroponics pH minus Grow + Bloom to pH 6.2 🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀🍪 🍒 🍰🍩 🎀

2022-09-03 I planted all 3 Seeds, started one indoors and 2 Outdoors 2 Outdoors were attacked by the Cat- and did not survive The indoor Girls developed very fast, and after 4 Weeks she went Outdoors too. There she showed great Performance. Easy to grow, no big attention needed. She was pleaded with her light prefertilsed Soilmix, and n eeded only afew Feedings in Stretch and i think one Bloom Feeding in Flower. After only 6 full Weeks of Flower, she war ready to Harvest. harbvesting was very easy here. Buds are thick, and dense. ( and sticky). The ratio from leaves to Buds are Phenomenal--- economic Plant---- nearly to no waste Only some branches, a handfull of leaves, and a big amount of lovely smelling Flowers The smell yes,,,, she smells like Pink grapefruit, and Peaches...... Stay tuned for smokingreport

Temp, Humidity & VPD are all controlled by a Mycodo System to reach a VPD Range of 0.5 - 0.7. Light is at its lowest setting 04.04. I started to add nutrients to the water. 2.5ml of Plagron Terra Grow per 1L.

Week 6 starts 12/31/21 but I happened to notice a weird leaf the night before. The Northern Light X Blueberry auto is very dark green and has sharp downturned tips. Which starts down the rabbit hole of google search to see the cause. I decide that is is Nitrogen toxicity caused by underwatering. At this time I was not really paying attention to when I feed them just whenever the pots were light. I watered them I later realize that while it may have started with underwatering it was so many other problems at once. I forgot to check the ppm or ph going in one day last week so I checked them going out. The run off was 2700ppm and I forgot to look up what that meant. I also doubled the strength of the nutes. 1/2 Saturday I flushed all the plants with 5 gallons of fresh water each that read 6.43 ph & 51ppm. I have Fox farm sledge hammer but for some reason I decided to use just water. The run off was consistently 6.2ph and I rinsed until the ppm was about 450 on NB. 250 ppm on Gorilla Glue and Pineapple express. I then decided that since it was nitrogen toxicity from underwatering that I should not starve the plant so I gave her week 1 of flower nutrients from the Foxfarm feeding schedule at 1/2 strength. I did not use Calmag since it's nitrogen was 2 and I didn't add the boomerang because that has nitrogen as a 1 and I thought since this plant has nitrogen toxicity I should skip the extra 3 nitrogen for now. I set each plant up on blocks so air could totally circulate to dry out the pots. Turned the fans on and waited. 1/6 There has been almost no growth since the flush. NB started off at a little over 9 inches and if I stretch her she is 10 at most.

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Nourri a l'eau uniquement.

She has reached day 70. I gave her water stress this past week. Weather has been warm with no rain. Larger fan leaves started to go limp and fade. Plant will be harvested in the next couple of days at this point in the diary.😍

second week of flowering done. They were stretching like crazy. Installed a 3rd net to keep the stretch under control. Jumped from 35 cm to 83 cm in 2 weeks. They giving me some headache because of the hight. Should have send them 1 or 2 weeks earlier into flower. But it’s a lesson how to handle or not handle hybrids and sativas. Over all in was quite relaxing just adding water/ nutrients and doing some defoliation. So far so good. it’s quite easy to keep them on the same level with 3 nets. Still learning how to handle certain situations and conditions. But so far I am happy. They have got a massive need of Kalium at the moment. Should have given them more TA -Grow nutrients. But that’s party of learning. Really happy about the aeroponic setup. Really like the full control of the grow medium. (Compared to soil it’s easier for control freaks). Really water efficient compared to dwc/hydro/soil and changes made take effect pretty fast. Really nice oxygen supply at the roots and overall nice control possibilities regarding to roots and medium/solution. A possible downside could be that u can only use mineral nutrients and no organic nutrients. (We want some bacteria and fungus but not these ones that break down organic nutrients. That is more a preference thing than anything else. Good made hydro can compete with soil easily but most ppl put to much in there and ruin it any way. (Don’t want to trigger its good that everyone got its own style and evolves it in his needs and conditions.) The maintenance effort that is required for aero is a bit higher than soil and dwc but it pays off. (Personal opinion but numbers proof it) Looking forward to the next weeks and flower build up. Really excited about. Conditions of the Grow: 💡 PPFD: 900 umol/m2/s ☀️ DLI: 38.8 mol/day 🌓 Lightcycle: 12/12 h 💨 Humidity: 50-55 % 🌡️ 🌞Daytime Temp.: 22-24 *C 🌡️ 🌙Nighttime Temp.: 19-20 *C 🌡️🌱Leaf Temperature: 21 *C 🌡️💧Water Temperature: 19-20 *C Water TDS: 1100ppm | EC: 2200us/cm 🚰Watersource: Rainwater (TDS: 15ppm | EC: 0.03 us/cm Vegetation Equipment: 🔦 Lamp: Mars Hydro FC-E6500 📤Container: 3x DIY 63L Euro-Box with lid Pot: Netpot 2 inch 💦Pump: M.R.S. Ultimate Whisper Powerpump 22 bar 🚿Nozzles: 12x 0.3mm 🫧Waterfilter: 150 microns 🌬️Fan: 3x Clipfan 🌪️Duct-Fan: AC Infinity 200 mm with Controller and Carbon Filter 🍶Nutrient Brand: Terra Aquatica 🔬Sensors: Waterstation (EC, TDS, Temp, PH, Salt), CO2, Air Temp. & Humidity (VPD), Temperature-Sensor Root Container

2022-11-13 All Solocups took grest, happy and green hey get a Feedingsolution packed with Nutes and beneficals, daily around 100ml each Solocup-or more, depends in their Needs The skullformed Bottle is wrapped to keep the Roots happy The Plant in the purple Cup is save by colored plasic hiding the roots website for for my Fertilisers: https://greenbuzzliquids.com/en/shop/ Code: mrs_larimar Breeder Info: 👉Black Sugar is an indica dominant hybrid of Black Domina, L. A. OG and Critical. It has kept the medicinal properties of L. A OG, a good flowering rate on the Black Domina lineage, and the compact size of the classic Critical strain. With its small growth, the strain produces large yields of up to 600gr/m2 on bushy branches. The buds are very dense and stinky. Black Sugar has a high THC level. The variety has a fruity citrus aroma. Dense smoke gives the effect of relaxation and body buzz. Good for Smoking before going to bed. It can become one of your favorite Seedsman strain Genetics Black Domina x L.A OG x Critical Harvest 450 - 500 g/m² Flowering 50 - 55 days THC 20.0% CBD 1.0%

They are an amazing plant to work with easy and robust they love the inviroment and are starting to smell amazing combined with the lights and climate control its a breeze don't have to do nothing the un topped one is a monster 3.5 ft hopefully fill right out thanks fast buds 💕

The Critical Mass CBD is lookimg good, tric's are clouding up. Keeping flushing with straight tap water. I am not ph'n the water, I figure this is the end and nute lockout should not matter. Would like to know everyone's thoughts on that. I am still waiting for some amber tric's before harvest, looks like it may be another week yet to harvest. I will have to move the plant to the edge of the lights again, new round going in. Thanks for looking.