Hello. This is the end of week 7 and the beginning of week 8 of veg. I took another look at the males and found them to be average. Not that smelly or resinous. So, no good for breeding. That's ok though. Let see what these females can do. They are topped already and ready to be repotted and put in the tent. There's still 1 plant undecided. Has small male looking flowers. I'll leave it for a few more days before deciding. Man, there are lots of large female orb weaving spiders around here this year. Huge webs, when you walk into them in the dark, they don't break off like the little webs. What a difference in design though. These spiders are all in my yard. I find them fascinating to look at. Hope your enjoying the comics. Keep Growing Straight. Chuck.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Day 85 23/09/24 Monday De-chlorinated tap water pH 6 and calmag today. Day 87 25/09/24 Wednesday Feed today using de-chlorinated tap water pH 6 only. Day 89 27/09/24 Friday De-chlorinated tap water pH 6 with Plagron PK13-14 today. Picture and video update 😎 Day 91 29/09/24 Sunday (End of week) De-chlorinated tap water pH 6 only today. Will try get another nutrient week out of her before flush 👌💚

7/15/2023 Day 11 Veg: watered again today, and added some Pure Protein Dry, BuildASoil Big 6, Rootwise Bio-Catalyst, and Yah-Whey Thrive. Also did a light foliar feed with the Pure Protein Dry as well. She still looks healthy so far. 7/17/2023: I appear to have a shore fly issue. This is a first for me. Delt with fungus gnats before, but these look like little flies. Need to start some IPM. Brewing a neem seed and mosquito bit tea, which I plan to do a soil drench with tomorrow. Will probably throw in some Dr. Zymes as well. I also have some beneficial nematodes I plan to add. We'll see how that works. 7/18/2023: watered with the tea I made yesterday, but instead of adding Dr. Zymes I added Lost Coast Plant Therapy. Also added the beneficial nematodes. 7/20/2023: the shore fly population seems to have declined, but it is still too early to tell. They are definitely in the earthbox, so I'm going to let it dry down more to see if that helps too. Roots are growing out of the bottom of the solo cup, but I'm going to wait another couple weeks before transplanting. 7/21/2023: watered with just plain water at a 6.2 ph.

I shouldn't have started the lst training on her because she started flower very fast and early so she ended uo being very small, which I hate because It has a very skunk citric aroma very powerful, I had a little harvest of 10g counting the little flowers which I used to make cannabutter to make some edibles.I wish I could have a huge harvest of this amazing strain, looking forward to grow her again. 🤞💚🌱💎

Had my run-in with powdery mildew midway through flower. But bud-washing was my savior! Completely killed all the pm and left me with a still incredibly strong end result. Its seriously some of the smoothest stuff I've grown to date, when smoked in a pipe.. I will be taking away 2 stars from the review because of the susceptibility to pm, however. This grow was a success as far as the autos go, overall... The photoperiod gal still has a few more days. Either way, I highly recommend this strain. Don't make my mistakes, make sure you keep your tent clean the whole grow through, ALONG with the air quality(just as important as the tent itself). That's where I honestly failed. After reading how spore transference works, I felt like such a knucklehead keeping my tent in a... 'homely' condition. Either way, lessons were had and I'm not one to make the same mistake twice! Cheers everyone, hope you all harvest some dankness as well 😀

my first coco grow...2 plants 1. northern light auto untopped in spider farmer tent 150x150 cm (5×5) with Aptus nutrients...this one goes out of control...let see if I can repeat this crazyness 2. northern light auto topped in spider farmer tent 70x70 cm and mainly TA nutrients Nutrients mentioned above are for NL topped (2.) in smaller tent (pictures follow) NL untopped (F-MILF) got this week the following: Aptus Topbooster 6 drops/l Aptus P-boost 12 drops/l Aptus K-boost 6 drops/l (this week added new) Aptus regulator 3 drops/l Aptus All in one 0.6 ml/l Aptus CaMg 0.6 ml/l TA Silicate 0.4g/l End of week 10 installed some self-watering (3 days off)...with coco problematic let see...final two weeks will be completely without any manual influence (2 weeks off)...hopefully staying alive

DIVINE OG KUSH / DIVINE SEEDS WEEK #23 OVERALL WEEK #10 FLOWER This was her last week she gave her all producing some dense frosty buds that have a slight purple hue to them. She smells of sweet goodness and gas!! Stay Growing!! Thank you for stopping by and taking a look it's much appreciated!!! THANK YOU DIVINE SEEDS!!! DIVINE OG KUSH / DIVINE SEEDS

:-)

Un terpeno que me recuerda a los años 90 Aquellos maravillosos

01/04/2022- Hello and welcome all been away on some family matter. So today was the last day this girl got watered and will also be the last day she got light as well, she will be cut down Friday morning. The smell has gotten a lot more stronger since flush was started last week Wednesday. Will update before I chop her down.

F week 4.

This is a very easy plant to grow, in my case i had some problems the last 4 weeks with heat that i couldn't resolve. I have had temperatures around 34 degrees Celsius when my light has bean on and around 25 when off.

It's pre flowering period, All organic, No nutrients, 50 w led bulb. See you soon in Mexico 👊😍🙏

Gave a light defol on the 21st, mostly on the California Orange Bud. It's been a pretty bushy strain this go around. Flowers are progressing on the younger plants and the older plants are starting to fatten a little.

DÍA 35 DEL esta es su última semana de crecimiento pinta bien 💚💚

So far so good , girls are coming on nicely . Nothing to report really. Afgooey is looking as fat as I hoped for