2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Hi guys So end of week 7 entering week 8. Blue cheese stinks buds are solid the dark phoenix scrog is frosty asf!!! They still have a way to go yet tho. I reckon by day 70 all will be well and done There under a 600watt hps light now to finish them off as using the led lights in my pot noodle grow off with wifey. I just started a YouTube channel I will be doing daily videos on my current run, like subscribe and comment on there for shout outs. Happy growing guys! 😁

2cm vertical growth this week.

this grow stretched a t least an extra week my last 2 grows were ripe in 8 weeks .There re no hermi flowers and its going to be at minimum 9 weeks mabe 10 to get ripe .dont know why it stretched into week 6 of flower when its normally 4 weeks stretching the buds are now getting filled in and solid just have to wait i guess

I'm not a big fan of gelato strech, she's a tall girl, had to supercrop a few more tops.

I hung dried for three days and then bagged them up in brown paper bags for two days due to the low temps and humidity before jaring them.

Budding like crazy under the new light,getting frosty. Plant 2 now catching up a bit,no purps though 😡 dropped temps. Around 20 days since first pistils No issues 🤞🏴󠁧󠁢󠁷󠁬󠁳󠁿🍁 🍁🍁🍁🍁 Breeder-White label purple bud auto 🍁🍁🍁🍁 Light-Voost VST 240(discount code TightNugs) https://voost.com/

-Seeds were germinated directly in soil 2cm deep with 60ml of pH'd mineral water and covered with cling wrap for humidity. -Lights cycle: 18/6 - Seeds popped after 3-4 days, 100% germination -Cling wrap removed after germination -Day 1 of grow is 8/10/2020 -Day 1-4 seedlings were feed pH'd mineral water -Seedlings received their first nutrient feeding on day 5 -Nutrient water pH'd at 6.3 and oxygenated with air pump + air stone -Mycoroot was fed via top dressing Struggled to get the pH with in range, I had to mess around with pH up and down to get it in the range of 6.3. The growth looks good so far just a bit worried up about the colour of the leaves. Temp varied from 26C-28C and humidity ranged between 42-52%. I had to play around with the oscillating fan speeds and positioning as well as the amount of air inlet vents. I found that just having one opened helped get the humidity up and the temp had only a slight increase. I let the oscillating fan run on its lowest setting and put off its rotating function, I also made sure the fan was not directly hitting the plants which assisted with the humidity micro-climate. The distance of the HPS was at 90cm from top of plants after the popped out of the soil, this was readjusted to 60cm once true leaves were showing. They got the first nutrient feed on day 5.

Day 14, Two of the Orange Sherbets are a little behind & the one I’m trying to pull back from nutrient burn.. So far the White Widow Autos by Seedsman have been doing the best! Day 17 two of the Orange sherbet are a little behind the rest of the plants but overall I would say everything is going good! Seems like the orange sherbet that had the nutrient burn is starting to bounce back! Day 19 They are all really starting to take off! Probably gonna do some LST tonight or tomorrow!

Day 16: What a good week for these girls. They initially looked like slow starters but soon have picked their pace up and now appear the biggest group of the strains growing as a whole. They are nice and green looking throughout and it looks like the LOS may be the leader so far. I am stoked with the strains I have running now and their speed is phenomenal. Seeing how quickly they have all changed in such a short time gives me a lot of confidence for their finish. Bring it on fast buds. Your impressing the hell out of me

Moved these 2 girls into flowering tent, on 11/1/24. Changed them from 15ltr pots to 20ltr insulated buckets. Gave them a defoliation & lollipoping today .

Flush at day 51. 10 days and I cut

A lot happened this week but she seems to be recovering and not worsening. I fed plain ph’d water end of last week but got a little too cavalier with too much top feeding and adding stuff without adjusting ph. All the top feeding is how debris and runoff kept getting into the base and mucking it up. I was out of homemade calmag so I had a bottle coming in the mail. I ended up adding calmag to not a lot of water and not ph’ing it and top feeding/pouring rest into the watering base later in the day after adding plain ph’d water. Wrong move. She appeared to slow down drinking but I chalked it up to a full base and plenty of top feed. Topsoil dried out very fast but I let her sit assuming she would start drinking eventually. This continued for 3-4 days, and also was happening to one of the photos I did the same thing to. Starting smelling something rank around this time so I checked the bases and found what is in pics 9+10. Ph was 8.5 and had this brown chunky film all over!! Took out all 3 bases and scrubbed thoroughly with soap and water and rinsed with some vinegar. I was worried about how dry and hard the soil/roots must be but I also didn’t want to do a full flush so close to her finishing. I opted to put 1/2 strength tiger bloom/big bloom and full strength calmag into full 1.8 gal water can ph’d to 6.5 and top feed slowly over ~20 min so it didn’t just rinse everything out. About 20% runoff which tested at 5.8 which seemed a little low but not as terrible as I feared. Cleaned the base back out after no more runoff and refilled with normal strength tiger bloom and calmag, ph’d to 6.5. This seems to have been the right move, 60 hours after flushing/cleaning/refilling, she has drank 75% of the base and topsoil is also not dried out. But we’re not done!!! The 2 photos also got a clean base and proper refill. The next day I checked the bases and found some insects on top of the water in ALL 3 bases shown in the last 2 pics. Teeny white things that kinda jumped on top of the water and seemed to stay underneath the fill port to get some of the light coming through? Hard to identify but I think they might have been springtails and not something destructive. Either way I made a weak vinegar/water solution and sprayed them aggressively over 24 hours and they seemed to die and not return. Only saw them in the bases and not anywhere on the plant. A lot of the leaf tip curling and spots spread from last week but seems to be contained and not worsening at the time of writing this. It seems contained to the top ~30% of the fan leaves and minimally on sugar leaves. While her buds did grow and thicken, I’m sure this set her back a little. All in all she had the water ph imbalance, definitely some light stress, nute burn and a little potassium deficiency. Got a little cheap digital microscope as well, trichomes still maturing with at least 30% still clear, plenty of white pistils as well. Some purple coming in on sugar leaf tips. Smell is much stronger. Idk if this is indicative of the buds as well or the “right way” but if I remove any small fan leaves I swipe the stem between two fingers to smell the oils. Still giving a very strong funky citrus scent with an earthy finish. Flipped lights to 12/12 for the photos at the end of the week. I just couldn’t wait any longer with how big the photos are. It also didn’t help that when I adjusted the light for the mimosa and moved it to the middle, the photos were getting much less and resulted in them stretching a lot with too much internode spacing. Not the end of the world but supercropping is definitely in their future.

Blue Black x Maple Leaf Indica x White Rhino x Ruderalis 70I-20S-10R So far no nutrients in the water as of yet. Starting to stink around day 19 of flower. Nice smooth skunky smell. Not exactly skunk. But smooth and sweet. Heat is not as bad but still pretty bad. pH is good. No visible signs of pests* or pathogens. *pests* little fruit fly looking bugs that don’t land but hover around. And a tiny spider trying to catch them. Web production from this spider has been extremely minimal, and when present, only existing connecting from leaf tip to leaf tip; On the fan leaf tips furthest away from the center. I haven’t removed the spider as it appears as an overwhelmingly insignificant problem, in fact: maybe a potential deterrent from other unwanted pests, like a protector/guardian. Either way I easily clear away any webs with my hands without even feeling or noticing anything. I think in the video, he is blurred out of focus on a leaf tip. Anyways. If the lil arachnid gets closer in towards the buds, I might consider moving him, but he is literally staying all the way to the outside perimeter of the plant. He is very considerate.

***** Week 11 growth - February 20 to 26, 2021 - Week 3 Flower ***** These girls sure catch my eye every time I open the tent! MM1 has some awesome hybrid vigour and is looking F’n awesome 👏 👏 Stacking up beautifully and based on the diameter of their tops there is some swelling to come. Getting the underside cleaned with a good leaf strip has really changed her look and lower sites are getting of lots light now👊 I could have gone harder but that was good for me.....I will deal with some of the lower shit that may remain. Light is at 20” and pulling 480 watts at the end of the week. Stated off with 400 watts at the start of the week but keep pushing it up this week. I don’t want to break 1,000 to 1,100 PAR by very much as I am not supplementing with true CO2. I have an Exhale bag to raise it a couple hundred ppm. Feel the need to keep the intensity high for penetration lower in the canopy. Will lower the amount of time in daylight if stress gets too high. Push to 11/13???? Nutrients are following IPP HOG 3 Part pretty closely. Just playing with macro nutrient ratios but otherwise supplements are at full week dosages following week 3. Have been seeing great resin production last runs so staying with Rezin as part of their feed schedule in flower. Terpinator......I think I see a lot of sulfer benefit here so it stays in the grows as well. Recharge seems to be working out well but we did the change mid grow from Microbial Mass. Since in coco have to feed the microbes so they reproduce and really dig Nature’s Candy with Remos additives in there👍 To help keep the root zone clean we add Enzymes😃 I have been debating a lot lately about the addition of CalMag to these nutrient lines and it’s necessity but since in coco I keep adding some......noticed a couple of spots on MM2 so have added it back in a bit more this week. Little more detail....... Feb 20/21 - Day 15 - Full feed today.....it’s been five days. - tap water with Silica @ 1.5ml, IPP line at full strength. - 1400ppm and 6.1pH - 4L for MM1 and 3L for MM2. Feb 21/21 - Day16 - Dry out day Feb 22/21 - Day 17 - watering with supplements and light feed today. We are in mid flower now.....need to keep the macro nutrients up. - Epsom salts @ 1 Tbsp in 20L. Purple on stems is getting prominent......could be cold temps but also giving magnesium. - Terpinator @ 3ml, Rezin @ 1.5ml, Nature’s Candy @ 1ml, grow @ 1ml, micro @ 2ml, bloom @ 2.5ml, Recharge @ 1 Tbsp in 20L. - 1400ppm and 6.0pH. - MM1 - 5L and MM2 - 4L. Feb 23/21 - Day 18 - keeping up the silica this watering - silica @ 1ml, CalMag @ 1ml, Ultimate B+ @ 1ml - 600ppm and 6.0pH - 3L for each girl. Little to no runoff this watering. Feb 24/21 - Day 19 - full feed again today.....girls are big and growing hard......keep it going💪 - IPP line as listed for full line. - Terpinator @ 3ml, Rezin @ 1.5ml - 1425ppm and 6.0pH - they are taking the high ppm feeding well. Feb 25/21 - Day 20 - Had some feed water left from yesterday. Added plain tap water to get 20L. - 810ppm and 6.1pH - 2L for each girl......don’t want to over saturate the medium. Feb 26/21 - Day 21 - Nothing today. - Will feed early tomorrow as it’s Saturday. - Let the root zone get some oxygen is my thought and water to nice runoff first thing in morning. Week three behind us looking forward to week 4. Expect to see more swelling and hopefully little stretch🤞 Frost levels are coming on strong and they have great signs for things to come💪😎 Pheno 1 is Turing out to be fire.....hope the terps to come are going to be as impressive. Thinking that these girls are going to primarily be turned into concentrates......or take a good size sample and see how she squishes👍 Glad we rolled the dice on this strain ETS.....now for patience😉

live now! FLOweek 6day41 she so cool! #fallen angel #clones #thaiweed #420bkk #livingsoil #organic #growergreenroom #ganja

Dia 47 de vida y tercera semana de trasplante se puede ver en sus enormes hojas, y en el tallo que la planta esta bien servida de nutrientes y elicitadores, van a ser unos verdaderos monstruos en 5 meses que va a durar el ciclo y vamos a dar mucha guerra !!

well, another week goes by and it's growing not like I expected hehehe but that's ok, living and learning, and for now I'll just wait for this cycle to end to start a new one and post it here and I'll tell you right away that I ordered a kit advanced nutrients starter and will test it on the new cycle. =D