Everything looking great . Got new lights today Mars TS 1000w Never mind all the mixed reviews online regarding these lights .. they are great ! No notice and there is not much heat off these lights. Also LTS tieing down branches at this point to get an even canopy later .. I prefer to have more smaller colas then one big cola

A new light has been added to the tent. I noticed a bit of a droop for several hours after adding it. After 4 hours they started picking up again. They are quite healthy and seem to be very happy. PH Is around 6.0. It just seems easier to keep it around 6.0. The PH seems to rise to 6 a day after correcting it down to 5.7 so I gave up. PPM is around 1000 and cal mag is being added to help them cope with the higher light intensity during flower. Pk13/14 has been added in a very small amount and a slightly higher does will be used in the next week. Please feel free to comment if you have any info that may help :) please take time to like as it helps me know if this is worthwhile updating. Microgrowery

had some lockout issues due to the pk booster.. i never use pk boosters. live and learn.

The girls are fully in flower this week which of course is a GREAT sight to see when opening the tent. The smell in the tent is so sweet and fruity it's already making your mouth water!! So this week I feed the girls with a little of the living soil veg so they are still getting a helping hand. They were also given the living soil bloom and some barley which I will admit is the first time using it but so far me plus the girls are very much enjoying!! I have done the necessary light de-foliating on all 4 girls over the past 2 weeks and more LST. Happy toking guys and girls!! Will keep you all posted with more videos through the week so please keep checking back 🙂

This little monster is shaping up nicely. This is the budget tent and she is directly under a single 60 watt HLG 3k panel now. There is a mars hydro 300 on the other side of this 2x4’. So far it looks like I’ve got 8 or 9 cola sites and I can’t wait for the stretch. My nutes are nectar for the gods organic and I’ve been giving her full strength veg on the intermediate schedule. Next watering I’ll switch up to the flower at half strength. I’d like to note that I’ve also been using bloom khaos in that line as a foliage spray but like with my other diary I’ll probably have to finish with kool bloom powder to get any sort of swelling. #dinafem # HLG #foxfarms

Day 26 of flower, plants are looking beautiful, no signs of stress

Progress is amazing she growing more tops everyday. I think all of my problems are now solved still have purple stem does anyone know why? heard it could of been a magnesium lock out but she's healthy in every other way. I'm desperate to put her in flower but think she will he to big for the tent!

Prima settimana di fioritura,tutto procede più che bene,,anche questi altri giorni si sta riprendendo ogni giorno di più. ..

Eternity Grow Cup 2025 with Plagron and Zamnesia 🏆 – Runtz Automatic from Zamnesia Seeds 🌱 Our beautiful lady is now 30 days old and standing proudly at around 28 cm. She has officially entered the flowering stage, and we couldn't be happier with how she's developing. Her leaves are healthy and vibrant, and she’s showing great structure and strength. She's been loving the nutrition provided by Plagron—a brand we trust for its consistency, ease of use, and excellent results. Daytime temperatures are between 25°C and 29°C, with humidity levels between 50%–60%. At night, it cools down to 20°C–22°C with humidity rising to 60%–70%, creating an ideal environment for her to flourish. Huge thanks to Zamnesia Seeds for the amazing Runtz Auto genetics—this strain is already showing serious potential. And of course, our gratitude goes to Plagron for their top-quality fertilizers that help us grow strong, healthy, and beautiful plants. We’re excited to see what the next few weeks bring—stay tuned!

Day 86 - Major training session on both ladies today. Things were getting too crowded with 4 photos (80+ days veg) and 2 autoflowers that both seem to have around a month left in them. Topped all upper branches and stripped most of the undergrowth, leaving only 2-3 nodes on every branch. Took me over an hour to do all four plants but in the end tings look much better in the tent. Now onto a hopefully short recovery on all plants. Day 89 - Both ladies are recovering from last training session. New growth is all lush and green. Very happy with both phenos of the Strawberry Chemdawg OG that I got. Remembering that I still got another bean makes me even happier!

Let’s go day 43!!!! Week went real well , girls started preflower so sometime this week I will be switching up the nutrients for flowering! We are stable and looking super healthy! Can’t wait to see what these ladies do this week! Hope you all enjoy !

This one was a f igther all the way , but she stilll gave her best , i will try her out again, i have her drying for 15 days with temps stable at 18 and RH also stable at 60% and it took her about 2,5 weeks to get to a point i like and trim. she is a deligth and super easy trimming and amazing sweet smell all over wen you do it , i mean just look at them photos , amazing. Over all i wold say stability 7 resistance 9 bag appeal 8 smell 10 taste 10 with leave us with a over all of 8.8 with is amazing for a tester not yet out on the market As always thank you all for stopping by for the love and support and for it all, i truly appreciate it all <3 <3 <3 Genetics - Fast Buds Tester 2308 Ligth - LUMATEK ZEUS 465 COMPACT PRO 
Food - APTUS HOLLAND #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #fastbuds #dogdoctorofficial #growerslove With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 
All info and full product details can be find in can find @ https://2fast4buds.com/ wen released 

https://aptus-holland.com/
 
https://autopot.co.uk/ 

https://lumatek-lighting.com/ Have a few good more diaries going on fell free to drop by and say hi <3 <3 <3 <3 <3 <3 Growers love to you all <3 <3 <3

11/20/2020 An early harvest! Will update next week after dry is done Currently wet weight is 110 grams. (final weight) and smell report then cure and final report after curing. 4-5 weeks? 11/24/2020 2nd Day of drying she dropped to 60.9 grams, day 4 I de bud the stems and jar her up total weight dry (bud only) 21.6 grams, under taste I'm going by smelling will test a popcorn bud and update but not expecting much this early in dry/cure. Ps. I looked under my scope and still got about 20-30% clear trichomes. So not sure how strong she will be but smells really good!. Three noses used for smells Nose#1 Fruity candy Nose#2 Sweet fruity mint Nose#3 Candy catpiss old school. 12/18/2020 I am very pleased with the results she is sweet smooth slightly citrus and evergreen No cough on this girl, high last a good.............................................. 4 hours long, no extreme thirst or reddining of eyes overall quite happy with her and looking forward to hunt the rest of these seeds! Taste, Smell, effect. Seeking a sweet vanilla cherry tastes in next run.

So where I live we had a really bad storm it had knocked out my power for a full 24 hrs so I had to give them some extra attention to insure there was no mold or any pest with no fan rotation. Then I also had to put them back on there proper timer to get things back on a roll so far so good they still look and smell amazing wish me luck

Day 77. Harvest day for that amazing plant. Dry trim chosen. Will update after drying. Thank You Zamnesia for that chance ! Day 86. Girls has started curing process. Happy Growing !!!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.