Die Ladys haben sich gut gefangen und erholt. War zwei Wochen in Urlaub, mein Kollege war nur zwei mal gießen und die wachsen super… bisher scheint die Topfgröße und das Volumen von 300L + gut zu laufen 😎 bin mehr als gehyped wenn die fertig sind… denke mache morgen ein scrog Netz drüber und breite die maximal aus bevor es in die Blüte geht

Flowers are still putting on weight. Very frosty and lots of bud.she is still drinking 3.5 liters a day

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Hello and welcome to the last week of vegetative growth. The ladies have been in the flowering tent for a day now and feel refreshingly comfortable at DLI 37.23 . Enough wind is provided . May Gaia have a productive effect . Day 32: +Pantine ++ 2.5 L Bottlewater (EC 0.315) (PH 7.5) +++ Rhizotonic +++ Terrar Vega +++ Cannazym +++ CalMag ++ Final PH 6.0 + EC 0.425 Day 34: looks like the ladies have a problem, the leaves are hanging

Germinazione effettuata tramite Jiffy, piantato il seme il 13/11/2022 alle ore 16:00 Primi segni di vita dopo circa due giorni, il 15/11/2022 Il giorno successivo praticamente tutti i semi iniziano a farsi vedere fuori dal jiffy Il 19/11/2022 Sono stati trapiantati nel loro vaso finale, 6 vasi da 20 litri e 3 da 11 litri Peso dei vasi da 20 litri senza acqua 6kg circa Peso dei vasi da 11 litri senza acqua 4kg circa

Posting the phenos when I do them

------GENERAL COMMENT------ Girls are moving forward, a lot of growth for the week, the two strains are absolutely different in terms of structure. Unfortunately the three Candy Kush did not sprout, so I will run only this two girls for this cycle. Down a bit the watering mainly doing a feed every other day, trying to manage the root mass to expand. Choose to do natures candy also in veg since i'm using recharge regularly, is 0-0-0 not noticing a lot of EC raise with it, velo kelp came in to play also. One dose of sensi Cal Mag this week since i notice some paling going on, my medium is inert by the way. ------SM.BLUEBERRY COMMENT------ She is creating a lot of foliage compared to the AK, both are in the same hight. May top in a week, this will depend.

Hope you all have a blessed holidays

Meno piante. Non metterò la rete ma topping più varie legature per mandare in fioritura tutte alla stessa altezza.. Speriamo di non sbagliare di nuovo perché tengo particolarmente a tutti questi semi e sarà l'erba che fumero' a Natale🤶Giorno 1/8 ore 4 del mattino messo a germinare 6 semi. 2 Kosher Kush (Reserva Privada) 2 Apex R1(Ethos Genetics) 1 Banana Sundae (Cannarado Genetics) e 1 Rainbow Belts (Archive Seed Bank). Giorno 5/8 ore 4 del mattino nati tutti i semi 😊😋🖐️

The ninth week of flowering is now over and the last days of the ladies have begun. The stated 9-10 weeks flowering time is definitely correct. One plant of the Hardcore Zoap was ready a little earlier, but as I want to harvest everything at once, everything is left standing until the majority looks ready. I also preferred to harvest the plants a little more mature than too early. In terms of appearance, I like the Miracle Gushers flowers best, as they have a fine and compact structure. The Banana Creamz seem to be able to develop very thick flowers, whereas the Hardcore Zoap seems to have the lowest yield. I will be able to say more after drying and weighing.

Harvest video Part 4. Please check it out and let me know what you think. Peace ✌️ Mr.PersonalGrow

Bueno ahí van arrastrando la defizcencia de potasio ya veremos a ver qué sale

Everything going great Auto daiquiri got healthier and they are climbing good. Made smooth defoliation, trying to keep schedule and humidity and temperature right. I AM happy and they look too.

Top strain, very easy 2 grow with amazing results in the end. Didn't try it yet, but when I hit it , I will definitely update on here. 1 more tree still waiting in line 2 be chopped. Will update pics once it's ready and final weight ✌️💚 So final results are 260gr of quality solid buds🤩✔️ that's not include the bottom trim and small buds. The whole harvest of 54 trees has the weight 5kg of solid quality buds, and around 2 kg of bottom trim and small buds🤩😶‍🌫️✔️

Has been a good week for the girls. They really exploded this week. I can't keep up with the ties. They got too big to have a level canopy. Will have to adjust later this week after defoliation and lollipopping. Will be a huge week and the girls will look different next time you see them!

Very good looking bush but wrong cal mag...

So far so good. Going into the final week of VEG. This week I have been busy with lollipopping the bottom some more and kept tucking multiple times a day to try to get that net to be full. I'll give it maybe 5 more days before flipping to flower. One of the plants was a bit too dark green IMO, so decided to flush out that pot to prevent EC running too high an risking nutrient burn. She seems to have been recovered from that.

it's amazing, this growth looks much better than my first attempt, my decision was to put a smaller pot and change the light - the girl really liked it, FastBuds goriilla cookies with a small space manage perfectly:).

I was worried about low humidity but both seeds popped on the third day! This is my second grow. I mixed some dried rabbit manure into my soil to release slowly. I have kept domes on the babies for now until they get a bit bigger.11/16 removed domes added a humidifier.