Big bud stop 32 day Overdrive start 33day Terpinator 1 of 10 days 4ml/l all flowering phase

Noch 1 Woche gebe ich der Pflanze, dann Flash Clean und Final Part von Terra Aquatica. Alles läuft fantastisch, hatte bis dato keinerlei Probleme mit der Genetik, auf Dutch Passion ist halt Verlass!

Continuing to water almost everyday as the pot size is too small and the buds are packing on the weight and frost. I am giving them almost 4 liters of water everyday. I am going to skip today (monday) just to make sure im not promoting any root rot, although I know I will be sacrificing some microbes. I will water them on my Live first thing Tuesday morning. Im noticing a little bit of bronzing on the leaves of one, its something I get almost every grow in flower. Im thinking it is beacuse I need to change my RO filters, as I live in the country and use well water and I think It is a buildup of iron. I have crazy high iron even when I use an RO. I believe my TDS was still 400 with filtration lol. If I change the filters I should be able to get it back down to 100 to 150 ppm. We are on auto pilot right now with this garden, just have to water and nurture and I know I am going to get an amazing result in about 3 weeks or so!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Super easy grow , i hst & topped this girl early & I think that really helped her out !!! She was grown in living soil so no NPK was given to this girl at all .. she smells just like lemonade, and finish on day 71 from seed !! Super happy with this girl ... she’s drying now , I can’t wait to smoke her!! She smokes super smooth, taste exactly how she smells!! Great job fast buds

Hello

Ok on flower day 65 i did a prechop of sticky smelly Buds. This is the Rest for Ripening. 04.10. Its boring to wait. Just added some water. Aphid shit on my buds (i wash), Or using insectoxids. Now you know why i can not use Insecticide. Another part chop. On day 71

End of week 3 Flower. She is spectacular!!! Buds are starting to ripen and the sweet grape aroma is heavenly.

This cultivation I lost a little bit the size that could reach . 😓 The light stayed too much close of the plant .

Going good 1 or 2 more weeks

Que pasa familia, aquí va la 3 semana de floración de estas Titan f1 de RoyalQueenSeeds. Tienen buen color y si siguen este ritmo van a salir fantásticas, a ver cómo pasa estas semanas cómo se forman las flores y cómo sigue formándose la planta en si. Temperaturas máximas de 27 y mínimas de 24 grados. La humedad relativa está en torno al 55% Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Hasta aquí es todo , espero que lo disfrutéis, buenos humos 💨💨.

Overall this girl was an amazing grow. I screwed up with her early on and she looked like she was on deaths bed. The soil was too packed and I didn’t put enough perlite in the soil mix so she was re-potted twice within a couple days and once I got the soil broke up a bit, she bounced back like nothing ever happened to her. Definitely looking forward to growing this strain again.

Overall everything is doing great. The Critical+ has the most trichrome coverage so far, but the DinaMed CBD+ has the thickest and densest buds. The Cream & Cheese CBD+ is somewhere in the middle - medium trichome coverage, medium density. But the Cream & Cheese CBD+ is the bushiest with the most amount of sugar leaves down the stalks with the buds. The 3 Cherry CBD Hemp plants are doing great also. They are the smallest 3 plants in group, but starting to show some red hairs. I think I'm about a month away from harvest. I'm not going to rush it, but that's what my guess is for now. I transplanted my babies from dixie cups to larger nursery pots. There are a variety of seedlings and clones going. I'll start a new diary for these babies soon with details on which strains, etc...

47th Parallel Crowly's Comet ╰⊰🔹Popped Seeds 03.10.24🔹╰⊰ 🌞Environment - 78F / 50% RH 🌾Training - Defoliating the buds as needed, a little at a time; they should be pretty clean by the time harvest comes. ⚱️2-Gallon 📊6.2 PH 💧 Feeding - Using Horti Late Bloom 0-24-26, Cal 12-0-0 🌞Mars Hydro FC-4800 🕷️ IPM - CannControl from Mammoth and Mosquito Bits ╰⊰🔹WEEKLY REPORT🔹╰⊰ 📝 Notes - I ran the High Fortified P&K of the Horti-Late Bloom 0-24-26 for 2.5 weeks; going forward, I will be watering with H20 PHed to 6.2. The flowers are becoming oily, frosty, and have a strong smell. The terpenes are producing a gassy citrus scent with notes of garlic and herbs. It's the strong, aging orange aroma - quite impressive! 🗓️06.08.24 Feeding 2x Dail with Horti Cal and Horti Late Bloom 🗓️06.09.24 Feeding 2x Dail with Horti Cal and Horti Late Bloom 🗓️06.10.24 Feeding 2x Dail with Horti Cal and Horti Late Bloom 🗓️06.11.24 Feeding 2x Dail with Horti Cal and Horti Late Bloom 🗓️06.12.24 Feeding 2x Dail with Horti Cal and Horti Late Bloom 🗓️06.13.24 Feeding 2x Dail with Horti Cal and Horti Late Bloom 🗓️06.14.24 Feeding 2x Dail with Horti Cal and Horti Late Bloom 📝Fertigation injects fertilizers into an irrigation system to supply dissolved nutrients and water to crops. ╰⊰🔹STRAIN INFO🔹⊱╮ Crowley's Comet / https://www.47thgenetics.com/product-page/crowley-s-comet-10-fem-birdseeds It's everyone's favorite piece of space rock, Crowley's Comet! The culmination of reversing our Matterhorn cut off Mr. Crowley to Intergalactic Runtz, and the results were out of this world (I had to do it). These ladies were slow to start, but once they hit their stride, they put any worries we had to rest. Compact, short-framed, and robust. They pack beautifully boulder-like flowers that reek of garlic and sickly sweet cotton candy. Dark green to a mosaic of purples, yellows, and silvers. Frost production is off the charts, the internode spacing is tight, and they certainly will impress in their last few weeks of flower. This is one of our favorite crosses in the new fem lineup. If you're looking for your hype fix, here you go. Yield: Heavy to XL Flower Time: 63 days Feeding Schedule: Heavy

I hung the full plant upside down for 24hrs. I then hung the individual branches. I left the branches big and I didn’t trim the buds as I wanted to dry slowly. They hung in the dark room with temperature at 21 degrees and humidity at 58% for a week. I then removed the buds from the branches and placed them in a drying net for another few day before jarring. Smell isfantastic.

All of the branches have been tied down for LST she is reacting super well so far. The smell on it is definitely becoming apparent. Love how they are reacting ti the nutrients from green leaf as well very responsive. Everytime i prune leaves of more just grow immediately pretty amazing. Cant wait to smoke this plant :)

10/12/22 watered with nutrient water. PH 6.6 TDS 400 10/15/22 mid-week plant is rebounding nice.

What’s up yall!!?? Week 6 and we got some serious buds growing in. Some of the sites that I thought wouldn’t produce actually surprised me. I haven’t changed much with this grow. I think the flush I did worked well and the plant seemed to react in a positive way. Trellis net and some LST just to get light down to the lower sites seem to have worked. No real change in nutrient and feeding. I do feed every watering so there I no skipping. I still use the General Hydroponics flora series and some cal mag. That’s literally all! See y’all in week 7

ABxRKO is about 42.5" tall, and has been top dressed with about 3tsp of grow dots to give it a phosphorus boost in flower HBSS is about 26.5" tall and has been top dressed with about 2tsp grow dots as I believe it is fighting a phosphorus deficiency. She is giving all the smells, a bright red starburst like fruity smell with a stark citrus note.

White Label Seed Company I hope this OG Kush makes it by the end of the week...I've been trying with some purple haze all 3 failed. This is 1 of 3 in the OG Kush I would love to see more leaf development for energy. Not a good start with her premature leaf structure.