Day 35: Good say growmies. I hope we are all well in these odd times. The ladies have been very busy this week with plenty of action on the L.S.T training and constant resetting of the stakes to hold them down. Rhe only girl that won't be trained is the sweet cheese. I am curious to see how she does on her own as she has lots of growth tips poking out all over main. She is still low to the pots surface and only about 10 inches at the moment. Most of that has come about from this last week. She is still only displaying her sex pistils ( no not the band!) , at present but is approx 1 week behind the other plants. Gorilla Girl #3 is doing well and not showing any issues. I did snap her main at the 4th node while doing the video but am hoping she can offer some repair time to the clean snap with my quick repair job..( ooops). Thankfully it is where she would have been topped anyway so the two nodes have now got a chance to flourish more. Gorilla Girl #2 is still the biggest of the group and has been pushing all her stakes out with her strength. She is in flower now without doubt but only by a few days. I am hoping the stretch period will boost her height now too. she has a lot of growth tips to become buds too. Gorilla Girl #1 is chasing the #2 for top dog and looks good. she has also just about started to show flowers properly now too. I expect she will put a lot more growth on before she settles into her mid flowering so I have high hopes for this lady. Gorilla Girl #4 Still the smallest of the bunch but I have given her a nice bend to help get her lower limbs active and wanting.. The whole group had a full watering to runoff a few days ago and so far , are more than happy to be left alone a few more days. Be safe and well

Harvest soon 🙂

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

She is fully in flower now and starting to stretch. She wasn't very thirsty, she only needed 2,4l of water this whole week. I again used 4ml of biobizz grow and bloom. I also removed a few leaves to make sure that every budsite gets enough sunlight, other ones I just tucked away.

La planta crece muy sana. Decido hacer poda apical en meristemos laterales, en 4 nudos. Al ser pequeños no sé si será apical o FIM. También un poco de LST en inferiores para que busquen mejor el sol. Estoy haciendo pruebas a ver como reacciona. Si veo que sigue en altura doblare un poco la a punta. Me planteo malla scrog. El riego continua siendo lluvia, una pasada los días de sol con pequeños momentos de lluvia.

Ginger Nut Cookies & Aussie Music Videos Fastbuds GSC x Barneys Red diesel 6 weeks of flowering completed 9th April 2020 Hi Everyone I hope you are all feeling well and keeping busy during the lockdown. It's raining cats and dogs here (lucky there's no chinese around LMFAO) and the humidity is 100% so today Ive chopped this little lady down and she is now drying up in my shed with fans going 24/7. I was quite pleased with the amount of bud I got off her and it's smelling fantastic and is very sticky.The trichomes were very milky with a few amber as well. 😍 very easy to grow no fuss and no problems but we'll have to wait and see as to whether she is a good smoke or not . Now for this weeks Aussie Music videos 👊 Because we are almost at the end of this grow I thought I had better add a few extra songs this week to make sure I get a decent selection of Diffrent music videos for you all to check out. So for this weeks selections I chose Empire of the Sun 👊 Walking on a Dream https://youtu.be/eimgRedLkkU Big Pig 👉 (from Bill n teds excellent adventure) 👊 I can't break away https://youtu.be/f_rfXVdYelA Peking duk 👊 Reprisal https://youtu.be/4GboBAg2Syo The white stripes 👊 Seven nation army https://youtu.be/0J2QdDbelmY Grinspoon 👊 Chemical Heart https://youtu.be/58mXxWuUqYw The hilltop hoods 👊 Leave my lonely https://youtu.be/yKifJ4Q5ph0 And Silverchair 👉Not bad for a bunch of 16yr olds which they were at the time this video was made https://www.youtube.com/watch?v=RZD982yrmx4 The Next update will be the Final harvest update with the dried n trimmed buds etc. I will also have to add another heap of tunes in that I had wanted to play , so keep an eye out for my next Update of Ginger Nut Cookies & Aussie Music videos .😀 Until then Stay safe and Keep lit And we'll all get thru this Together 🙏 👍

Day 50 😍welcome to week 7 😃👌 Day 51 i like the colorchange 😍🙏👍 Day 52 everything seems to ok 😃🙏👍 Day 53 and a long weekend 😎🙏👍 after 3 weeks a new SF SE3000 has to build in 💪😃👌

Der Start in Woche 8 ist regnerisch und bewölkt bei kühlen Temperaturen und hoher Luftfeuchtigkeit 😳 The start of week 8 is rainy and cloudy with cool temperatures and high humidity 😳 Day 62 "Haircut" for "Hilde" 😏😉

Week 3 of flower, can see already some nice bud production. Terpenes are building up and making the room stink, has a dark berry chocolatety gassy og smell to it, just how I remember choc mint. The bud structure isn't exactly what I was hoping for but cant complain! Was a free seed sent to me after months of searching for the strain at a time before the v2 was a thing. Buds will be going through the trim bag at harvest so will probs give them more a uniform look to them.

Hey everyone 😀. These weeks they have continued to develop very well 👍. They have made a huge leap forward since they were repotted 😃. They will be topped again this week, and will then move to the flower tent next week 😍. I am very curious to see how it will develop until next week. I wish you a lot of fun with the new update, stay healthy 🙏🏻 and let it grow 🌱🍀 You can buy this Strain at : https://sweetseeds.es/de/cream-caramel/ Type: Cream Caramel ☝️🏼 Genetics: Blue Black x Maple Leaf Indica x White Rhino 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

The first pistil showed up! Nice! I look at it 24 hours a day hahaha

🙊

The lady went into the drying tent this morning. I exceeded the suggested 6-7 weeks (flowering) by 4-5 days because I wanted to wait until she reaches 20-25 % amber this time. Despite all the issues caused by my mistakes, she managed to grow healthy looking frosty buds. This pheno was definitely not the biggest producer thoguh but the smell is absolutely top notch.

The start of week 5 is going well. Blueberry is smelling nice and my seedlings should be showing preflowers soon.

09/02/2019 Week 5 of flowering, got really sweet candy like smell,almost floral. Looks like she getting fatter fingers crossed!

Let's start growing the Z' Gelato😙 I place my seeds in water for 2 hours before planting them in a 0.5L pot of universal potting soil. 3 days later, I see the seedling emerging 🌱😄 I let my seedling grow for a few days before replanting it in its final 7L pot. she seems to like it there.😉 ------------------------------------------------------- My Instagram 🌱❤️️ : https://www.instagram.com/hou_stone420/ ---------------------------------------------- Equipment used : Light FC3000 Mars hydro. power 25% at 30cm Extractor 6 inch Mars Hydro. power 1/10. ON 24/24h No other fan this week Heating mat Romberg 95x95cm. ON half an hour every hour ------------------------------------------------- Thanks for your visite mate 🙏💪

Привет друзья. Наше знакомство продолжается с новым фотоцветущим растением от Smail_Seeds сорт ORIGINAL CHEMZKITTLEZ F1 reg. Сегодня растению 92 дня. Шишки наливаются уже тяжело ей стоять) Перевёл на 12/12 1.10.2023 Растение очень хорошо развивается, ни каких сбоев в генетике не наблюдается😀 Сорт выводим сами. Смотри мой профиль, у нас всегда есть что то интересное. Не забудь поставить лайк❤️, если понравилась как прошла неделя И читайте наш TELEGRAM: https://t.me/smail_seeds #Smail_Seeds 😀

Let’s Go Day 67 from seed !! So this week went real great! 2 Ogreberrys an Bruce Banner started getting flushed! The rest will follow up and start flush next week ! Today the 3 will continue getting flushed while the 3 others get there dose of nutrients, PHd at 6.5! Let’s grow lil ladies let’s grow!!! You all have an amazing productive day as well as a a great safe week !! Peace love and positive vibes to y’all Cheers 😶‍🌫️💨💨💨💨🤙🏻If there’s any questions please ask, I’ll be opened up to answer at best of my knowledge! Thank you all have a dank day !!

*Soil PH looks like it increased from 6.1 to 6.3 *Will check next day for more accurate PH *Increased PPM 350 to 450 *If size increases might top next week.