2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Sorry for the delay on the updates guys I am a daddy of a newborn baby boy and it's really time consuming plus the Mary ladies but we keep on the great work nothing wont stop us we go all the way up! Alright here you go Day 87 Starting week 13 Day 88 Gorgeous grapefruit smells like heaven Day 89 Again sorry for the late update but being a first time daddy leaves you without time Day 90 She's gorgeous isn't she? (alright guys so what happened here was that I started flushing too early and took advise from other canngrower and instantly added again all nutes, that's why I left her a lil longer until week 14 when I started to flush again, we are going to show you very well what we did, since it was our first time we were unsure if flushing at week 11 was alright but then we decided at week 12 that she could stay longer and she did) You might say we wasted some nutes but in terms of reality we did not, because we have a full bottle of 3.7gal mix with flora nova grow and flora nova bloom in the other, measuring ppm always to make sure we got the correct amount, otherwise we add more water or more nutes depending on the plant needs but we never waste away all precious nutes are precious resources, in this part of Latin America is really hard to find anything good, so we got to bring everything from Europe or United States that's why we doing the best we can with the money we got. Hey any donations are more than welcome if you guys like what we do, yeah it's risky around here but we don't give a F. You know that patients need their medical cannabis and Id say I'm one of them And by the way very proud Peace and love canna growers We will continue to fill the grow diaries

For LIQUIDS & NUTES ******GREEN BUZZ NUTRIENTS***** organic. Also i’m using their LIVING SOIL CULTURE in powder form! MARSHYDRO ⛺️ has large openings on the sides which is useful for mid section groom room work. 🤩 ☀️ MARSHYDRO FC 3000 LED 300W 💨MARSHYDRO 6” in-line EXTRACTOR with speed-variation knob, comes complete with ducting and carbon filter.

Let’s Go Day 81!!!! This week went real great 2 Ogreberrys are hanging to dry and also the Bruce Banner is too after there 48 hr of darkness! The 3 other Ogreberrys began flush on Monday so finish out this week of flush and one more week of flush after that an they will get the chop too! But stay stunned for next week , we’re not done yet so keep them eyes peeled!! Y’all have an amazing productive day as well as a great week ! Peace love an positive vibes to everybody Cheers 😶‍🌫️💨💨💨💨💨🤙🏻

Todo correcto por ahora 🤟💚

The heavy rain and winds are coming. And yes they have struck at my beautiful ladies. 2 trees were damaged. But with quick action and a nice dose of feedings. They do it just like before. The cellmax nutrition and Bionova. Do their job well. If you also grow on earth, I recommend it to you. Hold on for a few more weeks. I'm sorry if I make a mistake in the weeks. I'm doing my best to just make a nice update green greetings CaptenSmokey 😊👍

The Apple Betty Babies have been chugging along, doing their thing ❤️ The frost is starting! These girls are early producers, and seem to be responding well to the wind and light stress! The second trellis was definitely needed. They have been keeping to the same schedule of needing to be watered every 72 hours. I will be switching from cal/Mag to Mag/Sulphur supplements later this week. ❤️ how readily available these Advanced Nutrients are. The nutrient burn on some of the tips has not spread or gotten worse. The feed range seems to be the 700-750 range. Still keeping to the feed, water, feed schedule. I’ve increased the light intensity to 90% and will probably be stopping there. The temps have been getting as high as 81 degrees, and low as 73 at night. I’ve decreased the humidity to fluctuate around 50% , giving a reasonable VPD for most of the time, thanks to the automation of the in-line exhaust fan. Still have 4-5 weeks for these babies, I’m hoping they continue to plump up, they are showing some great promise. Happy Gardening 🤗❤️👊🇨🇦

Day 71 from seed the plant seems to be growing beautifully and now they are starting to develop beautiful flowers and they are entirely covered with white snow trichomes it is a very strong and powerful plant to grow what a beauty . thank you for reading I will continue to update have a happy grow.

Much better harvest to end this first greenhouse grow. 48g dry on this plant. Still quite airy, but resinous and great smell. More relaxed effect than harvest 1. I could have done with another week or two with her, but the humidity was regularly into the 90s, there was very little sun and nearby tomato plants were starting to grow mold. I cut and ran, I need these meds to get me through the winter. Final total for both plants 59g. With a bit of luck, this should keep me medicated for a while. Indications are good.

We are all finished! 79 days from seed, 11 weeks !

Welcome back all looks like the fun begins, week four is here and gone with the flowers staring to stack. It looks like my suspicion was correct and the plants needed a PK boost from flowering. I gave them a booster on the 10th and in the timelapse you can see how they responded. The K deficiency looks like it was halted from going any further and might have reversed it a little in the leaves. I had to add a little food after all in the mid to late flowering stage, but you have to listen to what the plants are telling you even if you think they have everything they need. live and learn.

It’s now showing pistils pretty well, and I got some critical peyote from Barney farms In a napkin right now There is a little burnt tip on fan leaves I think too much nitrogen since it’s in the big pot and the leaves are dark green. But looks good happy I picked the internodes during preflower too

Both laide are growing super well. One is taller but second one is more bush on sides. So far Im very happy grwoing this strain and both ladies just growing so nice

My favorite week, the buds are swelling nicely except for the platinum oreoz, they jus have long pistils. The colors are starting to come in. Did a small flush and defoliation.

This week have been all about making sure the bud sights get enough light and enough water. They drink rapidly now compared to the first 10 week of its stage. Very happy with the result i didnt expect to do so well with that many bud. So i am happy with this still and still as excited as i were in the start of the grow. I learned to be more patients with plants. I am planning on harvesting in week 17-18. This will be bombass weed i can see and smell it. Smells like a candy shop every time i open the tent :D

Semana 4, todo muy bien, Juana la mas grande me impresiona.. es hermosa! En cambio Gina, sigue creciendo con otro ritmo, pero de igual manera esta desarrollando todo su follaje.

doing great. we can see, Lance is a little behind, but he´s the one who got some issues in the first 2 weeks.

She is a little burned so I had to dim down the light intensity. I am almost defoliating everyday but still some bud sides are shadowed. Just following the Greenhouse Feeding Schedule and giving BioEnhancer every 2 weeks.

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