2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Today the 12/11/21 we start week 3 and I’ve been trying some light training on a couple of them just moving the big leafs from blocking the light to some smaller sights.they didn’t need water today either!!! Day 23-13/11/21 I’m trying out a bit of low stress training if that’s what I can call it I’m just moving the fan leaves so they don’t block we’re buds are gonna grow!!! Also gave them nice bit of water with biobizz grow today!!!! 1ml per litre!!!! Day 24-14/11/21 damn they got big since yesterday haven’t done anything today just letting them go wild probably put net on tomorrow!!! Day 25-15/11/21 nothing new today but there growing like mad!!!! Day 26-16/11/21 gave them a nice bit of water with bio bizz grow again!!! Day 27-17/11/21 nothing new today just watching the jungle start to form!!!! Day 28- 18/11/21 some of them are starting to bud!!!!!

Bienvenidos cultivadores de marihuana! 🖐️ 👨‍🌾 - Doce semanas desde que puse la semilla de Panamá Red a germinar! 🌱 - Diez semanas desde el trasplante a la maceta final. - Ocho semanas desde el corte apical - Cuatro semanas desde el cambio a horario de floración a 12/12H. - Tres semanas desde que comenzó la floración... a jugar! 😋 ¿Qué decir de esta mítica cepa? Ella crece con extrema fortaleza y a la vez con enorme discreción, sólo requiere la justa atención, riegos regulares y profundos cada tres días, yo le doy mezclas suaves y variadas con mis Bio Fertilizantes Orgánicos solubles, ella lo agradece mostrando brotes florales cada vez más bellos. 🌼🌼. Esta semana he quitado todas las ataduras a las ramas, una recompensa merecida! las ramas están preparadas para llenarse de enormes cogollos y soportar cualquier peso... me estoy desquitando de mi anterior cultivo de esta cepa! 😍 https://growdiaries.com/diaries/249559-zamnesia-seeds-panama-red-grow-journal-by-defharo Y por fin, hace dos días, la planta, generosamente cedió su esquina super iluminada a otra planta que necesita mucha luz para poblar sus largas ramas: https://growdiaries.com/diaries/286333-grow-journal-by-defharo Ahora Panama Red continúa su vida feliz en el centro trasero de la carpa de cultivo, un lugar calentito y bien iluminado... nada puede fallar! 🤣 😁 HASTA LA PRÓXIMA. SALUD Y SALUDOS PARA TODOS! ============================== PANAMA RED (Zamnesia): "Si te gustan los efectos eufóricos, sociales y creativos, tienes que probar la Panama Red. Esta variedad landrace sativa centroamericana te animará al instante y deleitará tu paladar con un intenso sabor cítrico. " - Genética: 100% Sativa Landrace - Tiempo de Floración: 11-12 Semanas - Hasta 350g/m² - THC: 17% https://www.zamnesia.es/10652-zamnesia-seeds-panama-red.html ==============================

De planten zijn naar hun nieuwe huis gegaan. Eerste week 12/12. De planten kregen wel wat root max schimmels en wat melasse en zeewiervoer Info van Zamnesia!! Green Crack F1 Hybrid Automatic: Zamnesia Seeds heeft een nauwkeurig afgestemd genetisch profiel; een combinatie van Skunk #1 en Afghani. Het resultaat van deze kruising: een betrouwbare soort die moeiteloos te kweken is en aanzienlijke opbrengsten oplevert! GREEN CRACK F1 HYBRIDE AUTOMATISCH KWEKEN Of je deze soort nu in een kweektent, kas of buiten wilt kweken, je zult versteld staan van haar aanpassingsvermogen. En dat is niet alles! De F1 Hybrid-genen in Green Crack F1 Automatic zorgen bovendien voor unieke stabiliteit, betrouwbaarheid en uniformiteit. Elk zaadje dat je ontkiemt, heeft het potentieel om vergelijkbare resultaten te produceren. Green Crack F1 Automatic wordt tussen de 60 en 90 cm hoog. Met ongeveer elf weken vanaf ontkieming tot oogst is Green Crack F1 Automatic niet de snelste soort. Desondanks zorgen die bonusweken voor een betere plant die uitstekende oogsten zal opleveren. Na een kweek met weinig onderhoud kun je opbrengsten verwachten van ongeveer 450–550g/m² (binnen) of rond de 50–200g/plant (buiten). Echt indrukwekkend voor een plant van dit formaat. AROMA'S, SMAKEN EN EFFECTEN VAN GREEN CRACK F1 HYBRID AUTOMATIC De invloed van zijn voorouders komt duidelijk tot uiting in het aroma en de smaak van Green Crack F1 Automatic. Geniet van intense, skunky smaken gemengd met een beetje GREEN CRACK F1 AUTOMATIC (ZAMNESIA SEEDS) FEMINIZED PRODUCTINFORMATIE Merk Zamnesia Seeds Parents Skunk #1 x Afghani Genetics Sativa dominante autoflower Bloeiwijze Autoflowering Bloeitijd 11-12 weken van zaad tot oogst THC 21% CBD Lage hoogte (binnen) 90cm Hoogte (buiten) 90cm Opbrengst binnen tot 550g/m² Opbrengst buiten tot 200g/plant Link naar de winkel: https://www.zamnesia.nl/10677-zamnesia-seeds-green-crack- f1-automatisch.html

Removing any yellowing bottom leaves from all plants. Blue Afghan has started greening at the top and buds are developing nicely. The Mac N Jack and the cherry garseeya are competing for fastest flowering, buds are gonna be massive and I have a feeling the Blue Afghan will catch up nicely.

Purple Ryder auto has been growing good under the Medic Grow Mini Sun-2. She did have a minor issue this week. So I changed the solution and hopefully have her up, and going strong again. Thank you Agrogardens, Medic Grow, and Doctor's Choice. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

She is a bit stressed. I just changed the solution on her yesterday. She was showing slight deficiency signs. So I just reset the nutrition solution. She bounce out of it soon. I have 2 fruity pebbles plant going in different nutrition. She has done a lot of stretching in last week as well. Thank you Medic Grow, and ILGM. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing https://youtube.com/@mr.sours420growingadventure?si=B9P0WhuqRMB0vbt-

Después de qué las dos sherbet no se dieron, aprovechamos la llegada de un par de Gorilla auto BSF y procedimos a germinarlas. Asi van al dia de hoy! 8/07/2024

The Gorilla Punch is still the smallest girl in the tent, but she’s really starting to show her potential this week.💪 She might not be big in size, but her structure looks great, healthy leaves, and a deep, dark green color that makes her stand out from the others.💚 She also got a light defoliation this week, just removing a few leaves that were covering lower budsites.🍃 Environment remains stable: Temp: 25–26 °C RH: 55–60 % PPFD: around 900–1000 µmol/m²/s under the Spider Farmer SE5000 Watering is on the same rhythm as the others, about 4 L every 3 days, RO water with CalMag as usual.💧 She’s small but healthy, compact, and starting to form some nice early flowers. Really curious how she’ll turn out, I have a feeling she’ll surprise me later on 😎✨

Update of week 2

Die Photos wachsen und die Autos blühen 😁 mit wechselhaftem Wetter und mittleren temperaturen.hat mir gut gepasst, da ich eine knappe Woche außer Haus war. Es riecht jetzt schon leicht nach Obst... Es läuft gut 👍 bis nächste Woche ✌️

Bien pues ya tenemos en marcha el 2º seguimiento del perfil... Son 6 white truffles, 6 Apple&Bazzokies de GBSTRAINS y 5 GG4 de GBSEEDS que se empezaron a germinar el 15 de mayo por la tarde sobre las 19:00 Todas nutridas con BOOM nutrients jejeje Todas las semanas iremos viendo los cambios y la evolución, los riegos y las flores que vendrán :) El 16 de mayo del 23 sobre las 07:00 de la mañana pase las semillas del vaso con agua, a los tapers con servilleta. Ahí estuvieron 24 horas mas haciendo un total de 36 horas germinando para después, pasarlas a las macetas de 1 litro. 22/05/23 actualizado, meto unas fotos mas de como van las semillas después de 7 días desde que se empezaron a germinar en el vaso de agua. A día 22/05/23 llevamos 7 días desde que se empezaron a germinar. ACTUALIZACION DE ESTADO: 30/05/23 Subo unas fotos después de 8 días. Llevamos 15 días desde que se empezó a germinar las semillas en vaso de agua. A partir de aquí, empiezo a contar semanas de crecimiento. Trasplantaremos a maceta definitivas de 7L dentro de una semana o 2.

my dry and cure style is this: 4 days of hanging upside down to get water activity lower to around 0.6 in 50% humidity and 26 C temp (i know its a little high but we are in a hot summer right now and i cant get it lower even with air conditioner) and then after 4 days of drying i remove leaves and stalks, trim buds and move them to jar for the rest of their life :D . and in the first 4 days of curing i open the jar door and let hem get some fresh air in the jar for about 5 minutes and close the jar door again, after 4 days of curing like that buds are smokable but they will get better as they getting cured about 1 month. buds are one of the hardest as fucking rocks type of buds! very dense , compact , sticky , smelly , amazing at every aspect growing stage was 56 days and flowering stage was 75 days total (harvested tops at day 64th) the total weight of dry buds was : (plant #1 & #3 top buds 56 G + lower buds 22 G ) 78 G + (plant #2 top buds 47 G + lower buds 18 G ) 55 G + (plant #4 top buds 120 G + lower buds 67 G ) 187 G = 367 G

No complaints this week. All the ladies are growing strong and healthy. Remember how stunted this plant was a few weeks ago? Now she stands taller than some of the other ladies. Speaking of, I have my 2nd trellis positioned exactly 12 inches a part from the 1st trellis. As you can see. There are many Colas that are over a foot long. I do believe that I will yield more from this harvest than the last. Only time will tell. I will keep yall updated in my next upload, Happy growing 🍃😎

Apr 6 - I'm adjusting ph on watering days - was using straight rain water, but it's ph is too high ~7.0. LST Northern Lights, reacted really well to it. Adjusted 04/07. Pink berry really kicking it up - 9 hrs into lights on, big difference from lights on. Bubblegum is a real beast. Just takes my mistakes. Apr 10 - Between apr 8 and 10th, there was a big boost in growth. Well ph'd water goes a long way. Pink berry is a beast, bubblegum phenomenal looking. Northern Lights doing good - you can tell I fucked it up in comparison.

Successful germination, actually started first seeds in June, popped 4 beans but during early growth I lost two seeds to over watering so I ended up with on 2 plants to fill 3 pots. Ended up trying 4 more beans in the same method, a couple months down the road,only lost 1 to over watering this time. So now I have 5 pheno's, 3 plants for now and 2 I'm saving clones of for later. Never had a problem with this kind of thing before so it speaks to the sensitivity of this strain. (More evidence later) start of actual seeds on this first run was August 18th.

Cant wait

the last day of light. Two days of darkness and scythe. 10 days only water