Week 02 Introduction This week has been a bit of a struggle. Had my first experience dealing with slime and brown algae. But I think adding enzymes and increasing the beneficial bacteria helped to fix the issue. Also experienced nutrient burn for the first time. My nutrient solution strength got as high as 671 PPMs. So backed it down to about 430 PPMs during the end-of-week reservoir change. Also cut Hydroguard (expired?), CaliMagic, and Superthrive from this grow. Now she's still stunted, but is starting to recover. Hopefully... [START OF WEEK 02] Day 01 - Monday - 08/10/20 - Total Days: 008 ----------------------------------- - [0830]: Still looks a bit yellow. But getting larger! --- Solution strength was at 248 PPMs. --- Just topped off with a bit of distilled water --- PH went up to 6.1 so PH'd down to 5.8 --- Ran the top feed for a bit to wet the hydroton. - [2130]: She's looking even more yellow now. --- Even the new leaves are really yellow. --- Decided to add more base nutes. --- Added 4ml of Sensi A --- Added 4ml of Sensi B --- Now at about --- Solution Strength went up to 304 PPM. --- PH was at 6, --- Also raised the water level a bit more. --- I really hope it's a nutrient deficiency and not an over-watering issue. --- Please live! Day 02 - Tuesday - 08/11/20 - Total Days: 009 ----------------------------------- - [0830]: Looking less droopy. But still yellow. --- Decided to do a reservoir change. --- 4.5gal of distilled water --- 8ml of Sensi Grow A --- 8ml of Sensi Grow B --- 8ml of Voodoo Juice --- 4ml of B-52 --- 4ml of CaliMagic --- 4ml of Hydroguard --- 4 drops of Superthrive --- PH'd down to 5.8 --- Solution strength: 302 PPM --- Since I didn't want to risk over-watering the rockwool, I did not to do a top feed, may do one tonight after the sleep cycle (lights are still set to go off at 10am and back on at 4pm). --- As I was doing a reservoir change, I noticed that the tubes and bucket were a bit slimy. Didn't really stink or anything. Is this normal? Maybe due to the Hydroguard and Voodoo Juice? --- I hope she gets better soon! - [2215]: Getting larger! But still yellow. --- Also can see a root growing through the net pot! Tiny little thing but it's there! Will have to start dropping water once more appear. --- After looking over a few DWC diaries using AN nutes, seems like my solution strength is still too low. Most around this stage average around 500 PPMs with about 4ml per gallon. --- Top fed for a minute to wet the top of the hydroton. --- Solution strength went down to 263 PPM. --- Added 8ml more of Sensi A --- Added 8ml more of Sensi B --- Now base nutes are at 16ml for 4.5 gal of water. --- PPMs went up to 411. --- Still lower than 500 PPMs, but that's okay. --- PH was at 6. Not sure if I should adjust PH using AN nutes. So going to not ajdust unless PH goes above 6.1. Day 03 - Wednesday - 08/12/20 - Total Days: 010 ----------------------------------- - [0830]: Looks like more root growth! --- She still looks yellow. But leaves are perky. --- PH was at 6.2 so lowered down to 5.8. --- Solution strength was at 394 PPMs --- Since roots are still growing, I decide to lower the water level a bit. Now water level is a bit below the net pot. - [2200]: Root is getting longer! --- Slime is returning. Not sure why. --- Solution strength: 357 PPMs --- PH: 6.1 --- Decided to add an enzyme along with more beneficial bacteria to hopefully cure the slime. --- Added 16ml of Sensizym --- Added 5ml of Hydroguard --- Added 8ml of Voodoo Juice --- New solution strength: 430 PPMs --- New PH: 6.1 --- Top fed for a minute. --- This slimy residue is really freaking me out! I hope the added enzymes will help eliminate the slime. --- Going to do a full reservoir sterilization/nutrient change over the weekend. Please survive until then girl! Day 04 - Thursday - 08/13/20 - Total Days: 011 ----------------------------------- - [0930]: I think the enzymes worked! There is little to no slime now! --- Although I think the root was also dissolved in the process. It's now just a little stub sticking just below the net pot. The enzyme is also suppose to dissolved dead roots too but guess that root was too weak? Not sure what happened there. But I think the slime is cured! --- Solution strength went down to 395 PPMs. Decided to add more base nutrients. --- Added 8ml of Sensi A --- Added 8ml of Sensi B --- Added 4ml of B-52 --- Solution strength went up to 598 PPMs. --- PH was at 6.1 --- As long as the PH is between 5.5 and 6.3, I'm not going to use PH Up or Down. But still will monitor PH levels. - [1715]: Checked up on her after her nap and shes looking alive but stunted. But no slime! --- Decided to add some sugars to help maintain the enzymes and beneficial bacteria. --- Solution strength was at 587 PPMs. --- PH was at 6.2 --- Added 8ml of Bud Candy --- Solution strength went up to 671 PPMs. --- PH also went up to 6.3 --- Lowered water level a bit to hopefully promote more root growth. --- Top fed for a minute. --- She is getting bigger, but still looks yellow. Day 05 - Friday - 08/14/20 - Total Days: 012 ----------------------------------- - [0915]: Leaves look a little burnt on the tips. Still no slime. --- Going to back off on the PPMs to hopefully fix the nutrient toxicity. --- Solution strength was at 632 PPMs --- PH was at 6.3 --- Added/Removed water until PPMs were at 536. --- PH was still at 6.3 --- Raised water up a bit, then top fed for a minute. - [2200]: Looking better. Still yellow though. --- Solution strength: 514 PPMs --- PH: 6.5 --- Added PH down. --- New solution strength: 518 PPMs --- New PH: 5.2 --- PH was kind of high. So decided to PH down. Had to use quite a bit to bring it down. But eventually got it to drop down to 5.3. A little low, but so far, the PH has always gone up so wanted to start it really low. --- Ran the top feed for a minute to re-saturate the top of the hydroton. Day 06 - Saturday - 08/15/20 - Total Days: 013 ----------------------------------- - [0830]: She looks stunted. leaves are still yellow. --- Solution strength: 514 PPMs --- PH: 5.6 --- Top fed for a minute. --- Not sure what to do at this point. Going to do another reservoir change soon. Hoping she lives until then. - [2145]: Looking pretty much the same. --- A bit of slime has returned. Since I've been diluting the nutrient solution, the enzyme ratio has dropped. So I guess this is expected. --- Also noticed that slime build up was on the air-stones. Never really checked the stones so not sure how long that has been there. Looks brown too, which can't be good. --- Going to do an H202 sterilization of the system (minus the net pot) tomorrow so did not add anything. --- Lowered the water level to below the net pot. --- Then top fed for a minute. Day 07 - Sunday - 08/16/20 - Total Days: 014 ----------------------------------- - [0900]: STERILIZATION DAY! --- Decided to skip today's night cycle for a deep clean. --- I'm hoping that skipping a night cycle won't be too stressful. --- Temporarily moved her to a new bucket. --- Used about a half of gallon of straight distilled water to flush out the net pot. --- Drained the system and wiped up all the brown sludge. Also removed the air stones and cleaned up all the goo. --- Filled the system with 2 gallons of distilled water and at least 30ml of food grade hydrogen peroxide (35% strength). --- Yea its a lot, but since I'm not going to sterilize the system overnight, I went with a really high doseage. Basically I kept adding until I started to see little bubbles start to form. --- Then I ran the water pump. - [0915]: Boiled the air stones in tap water for about 10 min. Then reconnected them to the airlines. --- I let the air stones and hose soak in the sterilization solution for about 5 minutes so the peroxide can absorb into the stones. Then ran the air stone to help mix and agitate the solution. --- Occasionally, I'd repeat the process of turning the air pump off for a few minutes to let the stones soak. - [1000]: Covered the top two row of holes of the net pot with some aluminum tape. Hopefully this solves the light leaking in through the hydroton. - [1200]: Used an old toothbrush and scrubbed the pump clean. Then drained the system. --- Refilled with more distilled water and peroxide. Again added a lot. --- Ran system again to sterilize even more. - [1530]: Drained the system and refilled with distilled water to rinse. --- Ran the system for a few minutes, then drained and rinsed again. --- Did this a few more times over about an hour. - [1630]: Added 4 gallons of fresh distilled water. --- Added 15ml Sensi Grow A --- Added 15ml Sensi Grow B --- Added 12ml of B-52 --- Added 16ml of Voodoo Juice --- Added 16ml Sensizym --- Solution strength was at 430 PPMs --- PH was at 5.6 --- I was going to add Hydroguard, but I think the bottle is expired. Since 2017 or 2018!? I think Hydroguard is good for up to 2 years from the date on the bottle or 6 months after opening. So not going to use from now on. I wonder if this could have contributed to the slime build up. --- Also removed the CaliMagic and Superthrive too. Going to only use AN products to eliminate the chance of incompatible nutrients. --- Placed her back in the bucket and top fed for a minute. --- Here's hoping for the best. Going to let it ride until morning! Please feel better! [END OF WEEK 02]

Questa settimana sta andando bene i fiori stanno diventando più grandi, sono tutti pieni di resina ma sono comunque ancora piccolini, speriamo che ingrossino ancora 🤞🏻 Aggiornamento 17 Novembre: Oggi ho aggiunto una nuova luce di supporto appositamente per illuminare i fiori che sono più indietro rispetto agli altri. La lascerò accesa h24 per vedere se può cambiare qualcosa, nel frattempo alcuni fiori sembra che stiano iniziando ad ingrossare e ad unirsi.

DIA 50; Comienza una nueva semana, durante estos dias habia tenido problemas con la humedad; dia 25 se instala Deshumidificador El día 25/06/2020 se han regado 3 plantas en macetas de 11 litros: X2 Semillas Auto Lemon Kix= 1,5L [1MlBLOMBASTIC+1mlKnactive+3ml/L ATAGrowth-C+5mL ATAFlower-C+] PH 6.4/ EC=1.84Tº=23 X1 Semilla Auto Tutankhamon= 1L [1MlBLOMBASTIC+1.5mlKnactive+5ml/L ATAGrowth-C+5mL ATAFlower-C+1mLAutoTop] PH 6.2(?) / EC= 1.84Tº=23 *El día de hoy se han regado 1 planta en macetas de 7 litros: X1 Semillas Auto Lemon Kix#3 = 800 ml [1MlBLOMBASTIC+1mlKnactive+3ml/L ATAGrowth-C+5mL ATAFlower-C+] PH 6.4/ EC=1.84Tº=23 *Se comienza a usar Bloombastic en semana 6, dia 42 (0.5 ml/Lt) Me gustaria realizar foliacion en esta etapa, sera recomendable ?

This plant is just monster similar to Haze plant. I have not done a lot of LST so there is one massive bud in middle. It's growing and growing but slowly I can see some frost on buds but this plant still have few weeks to go

This weeks update is just basically a photo/video dump! At the start of the week I did some lst and a tiny bit of defoliating and the growth just seemed to burst, I noticed they seemed to began to flower! So I went ahead and introduced the net to the set up and the I’m stoked with the way they filled this tent right out!! These cream manderin are truly fast growing buds. I also started adding Big Buds Liquid to the feeding schedule and so far, so good. At this point I’ll just be uploading photos every Tuesday/Wednesday 🤙🏻🤙🏻

En los últimos días algunas hojas están caídas hacia abajo si alguien sabe que paso por favor escríbanme y como puedo solucionar

1st week of flower....the girls are looking great and are all confirmed females yayyy...I will definitely a little and let the ladies be happy this gon be some good quality medicine

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

Nothing but colas on all 3 this is going to be a big harvest. Another 2 or 3 weeks max.

🇩🇪 Zum trocknen habe ich die Pflanze in drei Teile geteilt und sie leicht vorgetrimmt (Fanleafs ab) an Kleiderbügeln im Zelt aufgehangen. 13 Tage bei 62% Luftfeuchtigkeit und 23 Grad 🇺🇸 To dry, I divided the plant into three parts and lightly pre-trimmed it (removing fan leaves), then hung it on hangers in the tent. It dried for 13 days at 62% humidity and 23°C. 🇪🇸 Para secar, dividí la planta en tres partes y la pre-podé ligeramente (eliminando las hojas grandes), luego la colgué en perchas en la tienda. Se secó durante 13 días a 62% de humedad y 23°C.

Temps have been on the cooler side lately, with a few days close to 80° .

Well the grow was very pleasant, she got amazing structure an look of the buds, very frosty and amazing smell...so thank you Mephisto genetics you did insane job. i have ordered few seed from Mephisto but they lost i guess(((coz i have not receive them. So thanks guys for support and helping me for this grow all GD Family,Luv you all!!! Peace

420Fastbuds StrawberryGorillaAuto Week 9 What up grow fam. Weekly update for these lovely girls. They're getting really close to being done. The flowers are swelling up great with sweet smells coming off them. Trichs are starting to turn cloudy so I'll keep an eye and check them out later in the week. All in all very Happy Growing

Today I trimmed the tops of the plants. development is good. Tomorrow I will increase the amount of light. Day 14. Tomorrow I will start spraying with Delta 9 and get ready to start flowering. Let's move on. =)

🇺🇸Week 3🇺🇸 – Growth Explosion & First Training Back again for this week’s update! The Mother Gorilla is going crazy—she’s growing at an insane pace, and honestly, I couldn’t be happier. The plant is super strong, and the leaves are just massive. Some are reaching up to 21 cm long, which is wild. I don’t think I’ve ever seen that before. And honestly, I have to say—I’m seriously impressed by Formulex from Growth Technology. I’ve been using the same nutrient solution since the start, and the results are just insane. It’s such a simple product, but what it’s delivering so far is absolutely incredible. I’ll be switching to growth fertilizers soon (they’re on the way), but for now, I’m sticking with Formulex, and it’s honestly blowing my mind. Environmental Conditions Not much has changed on that front. The environment is still dialed in: • Temperature: ~22-23°C during the day, 18°C at night. • Nutrient solution temp: Holding steady at 17-18°C. • pH levels: Hovering around 5.8-6.0—had to adjust it slightly during the week, but nothing major. It’s stable overall. • VPD (Vapor Pressure Deficit): Still around 0.6, which seems to be working well. As long as it stays below 0.8, I think we’re in the sweet spot. • Humidity: Holding at 70%. Everything’s been pretty stable, and it looks like the plant is loving it. Major Work This Week – Defoliation & Topping This week was huge in terms of plant training. The foliage was getting super dense, so I went in for a heavy defoliation, removing all the big fan leaves. On top of that, I topped the plant—cut the main shoot to redirect energy towards the secondary branches. The goal is to push those side branches so they can take full advantage of the SCROG I just set up! Now, it’s all about recovery. Hopefully, she bounces back strong, and we can really start working that SCROG. Alright, that’s it for this week! Let’s keep an eye on things and see how she responds. Super excited to check in next week and see how she evolves. 🇫🇷Semaine 3🇫🇷 – Explosion de croissance & Premier palissage De retour pour l’update de la semaine ! La Mother Gorilla part en vrille, elle pousse à une vitesse folle et franchement, je suis super content. La plante est ultra vigoureuse, et les feuilles sont juste énormes. Certaines atteignent 21 cm de longueur, c’est complètement dingue. Je crois que je n’ai jamais vu ça avant. Et franchement, je dois le dire—je suis vraiment impressionné par le Formulex de chez Growth Technology. J’utilise exactement la même solution nutritive depuis le début, et les résultats sont juste dingues. C’est un produit hyper simple, mais ce que ça donne pour l’instant, c’est vraiment incroyable. Je vais bientôt passer sur les engrais de croissance (ils sont en commande), mais en attendant, je continue avec Formulex, et ça me bluffe complètement. Conditions Environnementales Pas de gros changements de ce côté-là, tout est toujours bien calé : • Température : ~22-23°C en journée, 18°C la nuit. • Température de la solution nutritive : Toujours 17-18°C. • pH : Entre 5.8 et 6.0—j’ai dû l’ajuster un peu dans la semaine, mais rien de bien méchant. Globalement, c’est stable. • VPD (Déficit de Pression de Vapeur) : Toujours aux alentours de 0.6, ce qui a l’air de bien convenir. Tant qu’on ne dépasse pas 0.8, on est bien. • Humidité : 70% constants. Tout est super stable et visiblement, la plante kiffe ça. Travaux de la semaine – Défoliation & Topping Cette semaine, grosse session d’entretien. La canopée devenait hyper dense, donc j’ai fait une bonne défoliation, en enlevant toutes les grosses feuilles. J’ai aussi topé la plante—j’ai coupé l’apex principal pour rediriger l’énergie vers les branches secondaires. L’idée, c’est de booster les secondaires pour qu’elles profitent à fond du SCROG que je viens d’installer ! Maintenant, elle doit récupérer. J’espère qu’elle va repartir de plus belle et qu’on va vraiment pouvoir bosser ce SCROG. Voilà pour cette semaine ! On garde un œil sur tout ça et on voit comment elle évolue. Hâte de voir ce que ça va donner la semaine prochaine ! 🤘😎

Nice to get the first month of flowering done! I believe these females will take just over another month, last 2 weeks of additives..

A tall one right off the bat here. I've never grown a strain with so much CBD in it along with THC. I'll enjoy watching this run, wishful thinking if everything goes right.

Kings Juice and CBD compassion are developing a nice odour. The Kuchi Diesel is developing a nice diesel scent with a shrubbery type branching, buds all over. The milky dreams is growing some popcorn buds full of trichomes. Jack Lemonade is nice long buds full of trichomes. The moweeto has a particular indica aspect in the leaves grows like a hemp plant and has nice bud development. Gorilla CBD is developing like a shrub, with bud sites all over.

📆 Semana 11: La Auto Pablo Escobar ha entrado en la fase de lavado de raíces, preparándose para la cosecha. Durante esta etapa, se suspende la fertilización para eliminar restos de nutrientes acumulados en el sustrato, lo que mejora el sabor y la calidad del producto final. Los cogollos mantienen su densidad y la resina sigue brillante, con tricomas que comienzan a tornarse ámbar, señal clara de que la madurez está próxima. El aroma se percibe más limpio, listo para la fase final. ¡Seguimos creciendo fuerte! 💪