Blue cheese auto day 86

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2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Almost into the home stretch. Will start looking at trichomes this week. Really hope the buds continue fattening on both the ladies. Day 58 WC - growing strong. Don't know if we should remove any leaves or leave it as is. Thinking of waiting a few days to see how the plant develops. Day 58 BCN - it's recovered from the lack of watering and the leaves are starting to turn yellow. Think that's normal for this far into flowering. Hope we can finish without further surprises. Day 61 - fed both plants and did an additional 330ml of blackstrap molasses (5ml/l). Will start moving to flush in 10 days or so. Not sure if they're ready yet. 🤦‍♂️🏼Added short videos of both ladies.

Auch wenn ich sie etwas früh geerntet habe ist sie stark genug. Der Schimmel ist einfach zu gefährlich. Dieser Bud Rot ist wie die Pest.

Start of week 6 flower | little bit of burn because light is so close | But just some very top leafs | started to purple up in some parts | smell is very permanent marker

Would love to hear from everyone..

Using mega crop nutrients only just started lst what do y’all think? Any suggestions?

Smell is Even more amazing now al swellin up pretty good Bluezy has a lil lighter bud structure for now but started swellin up just now Dantes Inferno has already some good nuggs Tomb Rider is really headbound and a lil too close to the lamp Starting to lower the EC from 3 to 2.5 Starting With fade 2 days earlier than planed will give them a 5 day flush instead of 3

One more week and she should be in flowering excited to see how she does been leaf tucking and lst best I can wish main stem was longer but hey it is what it is :)

Hallo zusammen 🤙. Sie wächst sehr schön und macht keine Umstände.

What's happening growmies, hope your well. These F1's are growing great fast, 3 out of the 5 are in pre flower power mode. I'll switch up the feed next week to bloom mode. Thanks for stopping by as always. Feel free to leave a like, maybe even a follow and I'll be sure to follow back. Stay high, stay fly. #420everyday.

Man any strain I grow from @fastbuds has the best all around terps of any auto flowering plant I have grown yet . They have strong genetics and a great customer service as well I've never been let down . To be honest when I popped this seed I knew she was special it cracked and got moving do quick that it was evident she wanted to grow . She was put into a 3 gallon fabric pot with living soil . I used NextGen soil out of Sudbury and the stuff is amazing. This whole grow was organic and the plant was at one point near death . I had over watered and let her go to the point that I wasn't sure she would make it . But she did and outperformed my expectations completely . The buds are long and dense with lots of trichomes . The way I fixed my issue was way less water and a couple top feeds of an organic flowering mix . The strength in this plant to turn around and come back strong like that was super impressive like no slow in growth and no hermies or lack of bud Growth . The newest strains that keep coming out are fire and I'm very happy to be able to try these at all . The next round of autos I do will again be of the fastbuds company . This plant in the end is around days 85 from seed and had it been trained better and more wattage could have been a super monster plant .. cheers and happy growing ..keep their plant going it's medical for so many people. Update smoke report - the plant cured nicely and the buds are hard with decent trichomes ! Not the sticky buds I expected but she was dried a little to long and so it's to be expected 🤣.. but in all honesty it's a great plant for bubble hash and I'm putting it into the hash bag mix for washing can't wait .. again cheers to fastbuds for letting me try their new strains cheers

Sweet 🤣

41 grams from this pheno

Sweet seeds thanks for super strain

Welcome to Flower Week 3-4 of Divine Seeds Auto Ak 47 Xl I'm excited to share my grow journey with you all as part of the Divine Seeds Autoflowering Competition 2025. It's going to be an incredible ride, full of learning, growing, and connecting with fellow growers from all around the world! This Lady moved into the 12/12 light Photo tent because of the height For this competition, I’ve chosen the Feminized Automatic strain: Auto Ak 47 Xl Here’s what I’m working with: • 🌱 Tent: 120x60x80 • 🧑‍🌾 Breeder Company: Divine Seeds • 💧 Humidity Range: 60 • ⏳ Flowering Time: 59 Days • Strain Info: 21-24%THC • 🌡️ Temperature: 26 • 🍵 Pot Size: 0.5l • Nutrient Brand: Narcos • ⚡ Lights : 200W x 2 A huge thank you to Divine Seeds for allowing me to be a part of this amazing competition and Sponsoring the Strains. Big thanks for supporting the grower community worldwide! Your genetics and passion speak for themselves! I would truly appreciate every bit of feedback, help, questions, or discussions – and of course, your likes and interactions mean the world to me as I try to stand out in this exciting competition! Let’s grow together – and don’t forget to stop by again to see the latest updates! Happy growing! Stay lifted and stay curious! Peace & Buds!

The temperatures, humidity, height, and watering volume(if measured) in grow conditions are all averaged for the week. The pH is soil pH. Any watering done by me is well water which is 7.6 pH and 50° F. Any listed nutrients are ml/gallon of soil spread evenly across the top of the soil. Day 1 we had a high temperature of 84°F. We had intermittent storms and showers throughout the day that took care of the watering. The powdery mildew treatment worked well on these. Day 2 we had a high temperature of 88°F. We had on and off rain and thunderstorms throughout the day. The rain took care of the watering. These responded well to treatment. I'll treat again tomorrow. Day 3 we had a high temperature of 94°F with clear sunny skies. I watered about 5 gallons from the well. I treated these again with Growers Ally fungicide. The warmer nights, blazing sun, and this fungicide have really cleared up that powdery mildew. They've also decided to go all in on flowering. Day 4 we had a high temperature of 94° with sunny skies. Heat index was over a hundred. It was super hot today. I watered twice about 8 gallons total. Everything looked good after treatment. Day 5 we had a high temperature of 95 and partly cloudy skies. I watered twice about 4-5 gallons each time with well water. Day 6 we had a high temperature of 91°F with partly cloudy skies. I watered twice today 4-5 gallons each pot, each time. These look great after treatment. Day 7 we had a high temperature of 88°F with sunny skies. I watered 5-6 gallons each pot. This week was a success. The powdery mildew issue has been cleared up. The Growers Ally fungicide worked great. These girls have rooted into the ground through the pots which is helping some of the 100+heat index days. It's hard to keep these pots moist in that kind of heat. Overall it was a great week.