This thing been a game

Growing well!!! Good progress this week.

Le sta facendo lei perciò è proprio brava per essere la prima volta Sta seguendo le mie direttive che le faccio da un po' di anni indoor... Vedremo alla fine,tra circa un mese Per adesso in video sembra bella, visto che non sono lì a vederle 😉 Buona Marijuana a tutti! 😅

This week went ok to. notice last time I updated my diary she started going into the flower so I've swapped to expert nutrients bloom with a ph of 6.8 . I don't think she is going to be anything too special which is okay because it tells me I have some work to do . I'm going to try breeding the seeds that I have with a larger auto at the end of year . Thank you for looking up the diary and will be an update next week 🌱👍

Now on day 49,47&41 flowering has started and they are looking lovely. Started adding 19-4-23 well water into every other watering ..

Vamos familia, actualizamos la quinta semana de crecimiento de estas Granny’s Home de Seedstockers, salieron las 2 de 3, 66% ratio éxito. Aplicamos varios productos de Agrobeta, que son increíbles para aportar una buena alimentación a las plantas. Temperatura y humedad dentro de los rangos correctos dentro de la etapa de crecimiento. La tierra utilizada es al mix top crop, por cambiar. De 2 ejemplares me quede con los 2 para completar el indoor y aplique tetra 9 vía foliar, también cambie el fotoperiodo a 12/12 y aplique una poda de bajos, se ven bien sanas las plantas, tienen un buen color progresan a muy buen ritmo por el momento. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

the smell is just ridiculous. I'm pretty proud of that. the buds are bulking nice, they are very dense and I am happy with the amount of bud I'm looking at. I go in a couple times a day to manually move the branches around to make sure there is air going everywhere a little, I pinch a few leaves here and there. Pretty much on auto pilot now, going to start watering twice a day now one nutrient and one straight water. Thanks for lookin! Also. my week count is wrong, my weeks are based on 8 nutrient mixes i use, i call them weeks 1-8, i start flushing my week 9, but for reference, this grow, week 6 ends on day 45, I'm more worried on the plant then the calendar. I have a 70 day general target from Garden of Green, I watch and change my "weeks" according to plant. I won't lie this grow looks and smells great to me, the smell is ridiculous how strong it is! and I am worried I will find excuses to cut earlier, lol, but i will fight that

02.04.2025 Tropicana Poison F1 DWC The plants are looking great the tipps of the colas at starting to get purple 💜 I need to invest in to another clip fan because of the humidity on the stem I will never grow in will blocks again ther are going to get reals nasty Hoping for a good harvest

Still no sign of flowering. Transplanted Novarine plant 2 into a 50-liters container. The spot is under a big shorea plant. Shorea is a South East Asian medicinal tree. This is minimum light spot in my yard. Hope she would flower. I choose plant 2 because I think plant 1 will stretch even further. Plant 1 was also moved to a shaded spot under an tree jasmin. This Millingtonia, Indian cork tree. I have sweet flowers all year round. People told me that dried tree jasmine flower mixed well with weed and is good for people with ashma and sinus. Kind of useless since why would asthmatic people even smoke? Plant 3 is my back up until I come up with a way to put her into flowers. Clone are doing nicely. And I am growing 3 clones from plant 1 and only one clone from plant 2. I’ll be donating the rest to friends with weight and blood sugar issues. By year end, I probable keep only one or two clones. One clone from bigger branch is growing just fine without any benefit form lighted grow box. Feeding plants with mid-level dose of cow manure tea and earth worm casting tea to keep leaves healthy. They should have been given a light dose of fertilizer. However, the flowering purslane are also eating and feeding soil microbes. Got my paws on triancontanol and brassinolide. Perhaps these will jolt Novarine THCV into flowering. Triacontanol won't dissolve in neither water nor alcohol. And it is emulsified with polysobate. Mixing of Triacontanol Triacontanol does not dissolve in polysorbate. It is rather emulsified. Means that it need to be melt and there need to be water added. My steps, 1) heated 70 grams of polysorbate in a big heat resistance glass cup. I used a beaker. Mix in 5 grams of triacontanol. And it does not melt. 2) Put in 100 cc of water and put in microwave. After the mix is boiled, triacontanol melt. There is foam. So a bigger cup and a spillage plate would prevent problem with my better half. 3) After heated, the mix is better. Then I added hot water from to make total 250 CC of solution. Then, the mix is clear. 4) Label the dose with pencil. This works better than labeling with ink. I use 5 cc at a time for my entire garden. 250 CC would last me 50 doses. No need to buy so much. This is cheap. Let’s see next week how effective they are.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

day 30/31 for the ladies

🚨 Durbin Thai/Cinderella 99 week 6 update 🚨 🚨 week 7 of veg!! 55 days old!! 🚨 So this is a clone I received on Jan 31st. She has been transplanted from a solo cup 2 a 5 gal pot. She had 2 be topped in her second week of rooting do 2 the top frying from to much light in young clone stage. Since the topping this lady has come 2 life!! She has been defoliated 4 times! Most recent defoliation 3/27! She spent her first month feeding just off nutrients in fox farm soil. On 3/2/22 I gave her her first Gaia Green trio feeding!! She has exploded with growth since!! This Saturday coming up she will get her 2nd Gaia Green feeding. 💡 Grow Sponsor💡 @marshydro_amazonled @marsnier #marshydro #TSW2000 Marshydro equipment-- Marshydro- 4x4 grow tent Marshydro- Tsw2000 Marshydro- 6" inline exhaust fan with digital temp and humidity sensor Marshydro- 6" carbon filter Marshydro- 6" Black duct line Other companies in this grow- @foxfarmsoilandfertilizer - 100% ffof @gaiagreenorganics - Organic fertilizer @inkbirdofficial - WiFi temp control @madmanplant- 6" Tent shelves @levoit.id - WiFi humidifier Thanks for checking out my grow!! See u next week!! Best of luck and Happy Growing!!🌱💚💨🔥🔥🔥🙏🏻🤟🏻💪🏻

💩Alrighty then Growmies We Are Back At it 💩 Well folks we just finished up the last run and so we are bad to do it all over again 😁 So what do you say we have some fun 👈 We got some Z & Z 🚗 🚘 🚗 🚘 👉 From Exotic Seeds Well we are just 21 days in and I have pulled her over and started some LST 👌 It's been another great week so I'm happy with what I got going on so far 👈 The stretch is on 👌 FC4800 from MarsHydro Lights being readjusted and chart updated .........👍I've added a UR45 to the mix👈 www.marshydro.ca 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

Hello, this week was good, all is on track. They are drinking quite a lot, and I had to defoliate a bit. The 2nd one is a bit late and must be different phénotype. I think they are very nice.

Week 7 had hit and the girls are now in flower, no problem that I could remember, I was feeding Dutch pro grow a+b and bloom but cut the grow out on week 7. 2 off the girls are massive and looking to be monsters.

This plant was a show-er for sure! The plants sprung up from the very beginning with huge leaves and thick colas! Nice purple hues and dense nugs! Would definitely grow again!

This week is about bud growth... Nitrogen has stop being used... Just removing dead fan leaves and letting the plant do its thing

This week went real great , one has been getting flushed and the rest we will start flush Tomorrow! These ladies are doin so amazing! Hope you all enjoy !! Stay tuned for next week! Cheers an happy holidays!