this week I did the top pruning to increase the production of the lower shoots to serve as clones when they are put to flower

Hi, topman! Yesterday we had defoliation big leafs, also was make regulation to stem of plant to the same high how its head. We have about two same weeks for change light of time on 12/12 for Part-Bloom. We are going to growing big place! You’ll check and looking at this report! See you, my dear topman!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Such a pleasure to grow her, she came from an auto m8 and she ended up being Photoperiod, apart from that I'm glad I've discovered her, because her buds have one of the most special and sweetest fragrance I've ever smelled in a cannabis flower, super sweet and tropical guys, such a cool strain, I definitely want to fill a lot of jars of this for my personal stash, everytime I smell her flowers makes me remember the Caribbean Islands.

08/21/2018 Day 1 week 2 of flower. Last bit of supercropping, keep the canopy low as possible 08/22/2018 #5 is the only 9 fingered leaves plant, all other GTH plants have 5 & 7 finger leaves, #5 took 3 clones she's my keeper of this batch. She is very diesely citrus,musky smell.can smell her at 90". #4 similar smell not as much stink. #3 minty chlorophyll smell. #2 orange,citrusy,mint. Ps. Gonna hit a selected branch or 2 of GTH #5 with mystery cookie males pollen I saved from earlier pollen harves. (week 19 mystery cookie grow) & some 2017 cherry pie pollen.( See Breeding Project, pollen test)@growdiary. #1 most similar to #2.rubs Smells from stem rubs on #1#2#3#4 , #5 no stem rubs needed she straight up stinks.👊🔥🚀⛽♨️

She looks totally awesome check out her leaves and side growth!!!! No sign whatsoever of male flowers from my S1 seeds!

What's good in the hood growmies!!! I hope all your grows are going well. I missed week 8 and week 11 picture updates due to my busy schedule. These pictures were taken Friday 8/19/22 which was the start of week 12, I finally got around to posting them today which is Monday 8/22/22. Week 7 of flowering, everything is going as planned. I will give these babies the chop in a week maybe 2 max. I have been feeding them every 3 day which seems to be when they need a refill. Starting this Friday, I will only feed them plan PH'd water until they get chopped that is my way of flushing them, I like to do it progressively rather than flush all at once. It has been a very stress free experience for me, I feel like I have the game down pat as far as growing from seed. I still have my Caramelicious Feminized Photo mother plant vegging and growing and also White Widow#3 (which is 3 weeks behind the other White Widows) I just haven't been taking pictures of them. Thanks for stopping by and I hope the plant goddesses grant you huge yeilds on your grows!!!

(Batch#1)Nothing much to report this week. All plants are growing steadily and healthy.Most plants doubled in size and grew wider.

👉Alrighty Then👈 We are now just around the corner , looks like it's almost chop time 👏 🤪 We are almost there 😀 👉 I've continue to emend my nutrients plan i am now dialing it down to flush ..... 👈 😳So I will be showcasing just 3 this round even thoe I am growing 7 plants 😳 👉Black Domina From Weedseedsexpress 👍 👉Permanent Marker From Weedseedsexpress 👍 👉Sleepy Joe OG From Weedseedsexpress 👍 The other 2 are repeats cause they are pure fire 👈 You can view these 2 in the 👉Summer Classic Diary 💪 Had issues with the Banana Biscotti germination, so it's way behind and also the extra seed I popped 👉Purple Oreoz From Seedsman 👍 👉Persian Pie From Greenhouseseeds 👍 👉Banana Biscotti From Seedsman 👍 👉Gorilla Cookies From FastBuds Soil by Promix Nutrients by Cronks Lights by MarsHydro & Vivosun Well this should be fun 🙃 Thanks to all my growmies out there for stopping by its much appreciated 👈 👉Happy Growing👈

Flushed the res today, switched the nutes and flipped my light to 12/12. Its fair is now in the hands of the gods haha..., Will upload some pics after their first 12 hour sleep in 9 weeks!!!... This things sure going to stretch during flower, every day each tops is stretching to the next square on the scrog. . . The Gorilla is stretching and looking lovely, nice dark leaves oozing oils and aromas, love this girl/boy haha... . Last night of week 1 flower, plants looking awesome, all potential bud sites reaching for the light from the scrog.

Hi everyone 🤗 This week the Orange Sherbert Phenotype # 1 and the Zkittlez Phenotype # 2 were harvested :-) Now the Victory Kush 1 - 3, Zkittlez 1 - 2 and Orange Sherbert 1 are ready to dry. All others except the gelato are rinsing 👍 Orange Sherbert 2 will be harvested next week :-) Until then, I wish everyone a nice weekend, stay healthy and let it grow 🍀🌱👌

Just as difficult to grow as the first time I done Afghan kush but still managed to get some nice purple bud out of it!!!

Day 92. All looking good. They will be getting Flushed from now on. Canazym to help flush roots. Rezin for some full taste. Day 93. Everything on point. Letting them do their thing; waiting for the leaves to get more of a lighter green. Day 94. Flushed Fed 4 L - 4.5 L each with 10-20% runoff. They are finishing up; Fan Leaves are yellowing and drying up. Trichomes are milky and really Amber on others. The 2 Diesel are still behind by about 10 days when compared to others. Day 95. Waiting for some to finish up. Will be harvesting in the next 10 days. Day 96. Flushed fed 4 L - 4.5 L each with 10-20% runoff. Day 97. Some fan leaves on some plants are yellow with brown spots, looks bad but they are comming to an end. Not worried. Day 98. Flushed fed 4 L - 4.5 L each with 10-20% runoff.

Soooo week 10 :) These girls are looking reeeeaaal nice SS1 (on the left) is <25 days from harvest and SS2 has still a while to go id say 😄 But all in all things are looking super good buds are looking super nice and frooosty with SS1 starting to shift its energy into budding whereas SS2 is still throwing out some wild pistols :) The smell is super strong which I’m happy about, some staaaanky skunkalicious bud🤤 I have stopped giving SS1 nutes, giving her only water after her flush in week 9and she’s doing awesome! SS2 I’m still giving biobizz grow and bloom 0.5ml/l ea. And she is also loving her environment Got some mushies growing in the one pot though 😄 pulled them out just to be safe still have to do some research :) might have something to say about the enviroment at the surface of the soil. ??

Hi, I have a little problem with powdery mildew after the rain, so I removed a lot of leaves for better ventilation and easier handling, the smell is amazing, while removing the leaves my hands were all in resin.

Time lapse 23rd June > Aug 21st 2020. Plenty of screwups - over/under watering+ feeding + pollenated the tent with one hermie, two plants throwing nannas (due to UV) out in the last two weeks and the major heatwave all did not help, This was totally my screw up and for that I still give this strain a solid 8/10. The tastes are nice although my first try of Runtz was in Cali and it is certainly another level over there. Unfortunately I did not get a pheno similar to that I smoked in the US but out of the 5 I did get I really enjoyed one of them which is hard hitting! Apologies for the time-lapse as its not always spot on and often other things took over. Stuff never goes the way you plan it in your head. Upon drying the temps were good and they dried okay. The reason why this is a write off is because one of the plants hermied and caused most of the plants to be pollenated. When I harvested I noticed a few nannas poking out so I'm not sure if I let them run too long, too much stress, underwatering, light intensity, the UV bar or heat stress. Anyways I didn't catch it and I have some well developed seeds. I think it's down to the UV bar causing the nannas but the seeds were ripe (tiger stipe brown) so didn't have enough time to grow and ripen. Seeing as I had deficiencies early on in flower - WK4 - I think it happened then. The PP and runtz all have seeds bar one plant (the nice gassy pheno) and the tent was cleaned with bleach, vacuumed and temps increased to over 100f with a heater after disinfecting. Nevertheless I will grow this again! it's all a learning curve! More bud shots to come when cured. Just over an 1oz per plant is pretty poor imo but again you live and learn from your screw up! Got to be on point with my watering next time! That's why I love hydro!

Endlich haben sich die Geschlechter eindeutig zu erkennen gegeben ... Ich habe eine Dame ausgewählt - Es war Dame Nr.1 : sie war die erste die gekeimt hat und die erste die eindeutig geblüht hat - Der Rest waren auch Mädchen : die habe ich in die Mülltonne entsorgt ! Dame Nummer 1 wächst recht schlank und aufrecht / locker - Ich hatte sie über der 4. Nodie getoppt .... Sie riecht herrlich nach Citrus und ein wenig skunkig ....

Día 44 (15/07) Wow! No hay shock de trasplante! 😍💥😁 Todas las ramas se han estirado hacia arriba tras el ajuste de LST y se están formando nuevos nodos rápidamente Esta combinación de nutrientes de Lurpe + substrato de PRO-MIX HP es una maravilla 😍 Día 45 (16/07) Ayer tuvimos ola de calor (37 ºC) y han aguantado muy bien! A última hora de la tarde (cuando ya no había sol directo) les apliqué H2O en spray foliarmente para refrescar los estomas Hoy parece que vuelven las temperaturas estándar para esta época del año: 29 - 30 ºC en las horas centrales del día Riego con 1 litro de H20 pH 6,5 Día 46 (17/07) Pequeños ajustes de LST Hay algunas ramas inferiores que no se han formado / estirado mucho Lo más probable es que haga una buena limpieza este domingo, 7 días después del trasplante, para dejar solo los brotes por encima del 4º nudo Día 47 (18/07) Riego con 0,5 litro de H20 pH 6,5 Este domingo (día 50) haré limpieza de partes bajas y tal vez también de nuevo topping en las 4-6 ramas principales 😁 Día 48 (19/07) Día de muchísimo calor con 37 ºC. A ver como lo llevan! Riego con 0,5 litro de H20 pH 6,5 Día 49 (20/07) Para ayudarles a llevar mejor el calor de las horas centrales del día, coloca una malla de sombreo (70%) sobre el invernadero Lo cierto es que se nota que baja mucho la temperatura debajo de la malla! Van a estar más fresquitas! 😍 Riego con 0,5 litro de H20 pH 6,5 Día 50 (21/07) Training session! Hago limpieza del tercio inferior de la planta, de todas las ramas y nudos que no superan la línea del dosel marcada por el 4º nudo (1er topping) También hago topping (2º) a todas las ramas principales La idea es tener 12 ó 14 colas principales para que no se hagan muy altas De paso saco unos cuantos clones de las ramas inferiores eliminadas 😁💥 Para superar el estrés, hago las siguientes aplicaciones y riego: - Riego con 1 Litro de Té Vegetativo de Lurpe Solutions. Preparación: 24 horas con bomba de aire (oxigenación) con ingredientes: Green Sunrise 8 ml/L + Insect Frass 16 ml/L + Hummus Lombriz 8 ml/L + Melaza 1 ml/L + Kelp Hidrolizado 0,25 g/L - Aplicación foliar Kelp hidrolizado de Lurpe Solutions a 0,25 ml/l 💦Nutrients by Lurpe Solutions - www.lurpenaturalsolutions.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

Candy gas ⛽️ 🍬