Fastbuds Meme Video Contest

Weather has been much better this week. Put in some nematodes to try and regulate on some fungus gnats. No feeding yet this week. Topped the Deep Purple this week, and took a little to much off the top lol 😂 but should be quite all right. Gonna start some LST this next week. Super fun and grow on 🤙🏻

Thaught the yield would be bigger and the smell louder in generell.

Another hard defoliation at end of week 2. Mixed new reservoir for the week, with Cropsalt it remains stable for over 7 days if you adjust your PH, stable for 24 hrs, prior to mixing your nutrients. Will add more pics throughout the week, 3 genetics, Apple fritter X thin mint, cereal milk cross to Runtz and Natty’s Kush x thin mint (GSC) All are in 2 Gal, coco coir/perlite w/ Dynomyco 🚀🚀🚀🚀🚀🚀🚀

Eternity Grow Cup - Week 11 VEG Report This is a pivotal week in our Eternity Grow Cup journey as we wrap up VEG and prepare to transition into FLOWER. Our goal has been to ensure that the plants are thriving and optimized for the next phase. This report will detail everything that took place during this crucial week. Mass Defoliation & Structural Training We began the week with an extensive defoliation session. Every leaf with a stem was removed to clear space for optimal light penetration and airflow. This process was done mostly by hand, clicking them off in the opposite direction of growth, while some stubborn ones were snipped with scissors. By the end, it felt like we had taken out nearly half a kilo of leaves! The room now looks clean, structured, and ready to fill out the ScroggNet. To further enhance the plant structure, branches were attached to the pots, allowing for better horizontal growth. This technique helps maximize canopy exposure and prepares the plants for even light distribution once flowering begins. Defoliation before flowering is a key step as it reduces overcrowding, improves airflow, and sets the plants up for efficient energy use in the next phase. Light Schedule & Adjustments This week, we introduced a subtle but effective light adjustment. While still maintaining an 18-hour light cycle, we initiated a 15-minute delay with the whites, allowing the reds to start first. This gradual shift signals the plants that a change is coming. Similarly, at the end of the light cycle, the whites turn off 15 minutes earlier, leaving the reds on for an extended period. This strategy mimics natural sunrise and sunset, helping the plants transition more smoothly into flowering. Additionally, at the end of the week, we will be implementing 48 hours of darkness before the official flip to flower. This method has worked exceptionally well in past grows, helping plants recognize the transition and encouraging a strong start to flowering. However, it's important to emphasize that this is a personal technique and not a universal rule. Every grower should experiment with different methods to find what works best for their specific environment. Environmental Conditions & Challenges Currently, our temperature is at 27°C with 60% humidity, resulting in a VPD of 1.50. While slightly higher than the ideal for VEG, our CO2 levels are over 1000 ppm, and the light intensity is at 570 PPFD, making it a manageable balance. The solution pH is 5.79 (essentially 5.8), EC is 1.38, and water temperature is 17.7°C. One challenge we are facing is maintaining optimal reservoir temperatures. Currently, it's a bit low at 17.7°C, and we are exploring solutions to raise it slightly. However, the medium temperature sits at 22.2°C, which is a good range. The humidifier is still in use, though it struggles to increase humidity further, so we are working with what we have to keep the VPD in check. ICL-300s Integration The ICL-300 lights are now fully active. Unlike the last grow, where we introduced them later, this time they are on from the start of flowering prep. This adjustment is an experiment to see if the plants respond better to an early introduction. Learning is at the core of this journey, and each grow offers new insights. Whether this proves to be the optimal strategy or not, it will be a valuable lesson for future grows. TrolMaster Hub & Data Monitoring Throughout the week, we have been closely monitoring our grow using the TrolMaster App TM+. We are displaying some of the collected data for everyone to see the details. Stay tuned as we explore this further in the next report! As always, we want to extend a huge thank you to our sponsors and the amazing community. Special thanks to Aptus, Groovebags, Cannakan, Promix, Ziggy Papers, TrolMaster,and Plagron, Zamnesia and Grow Diaries for making the Eternity Cup possible. I apprecilove the support from all sides—lovers, haters, competitors, and fellow growers. The competition continues to push us to improve, and i am thrilled to see the incredible reports from others. This is a challenging yet rewarding experience, and i couldn’t be happier to be part of it. For those following along, we invite you to check out our YouTube channel, where we upload full 4K episodes showcasing the entire journey. Some episodes are too large to be uploaded directly to Grow Diaries, so YouTube is the best place to catch everything in high definition. This marks the end of VEG, and we are officially ready to flip to FLOWER. Next week, we will dive into the TrolMaster System in action, check the data insights, and monitor how the garden adapts to the shift. Stay tuned! Growers Love to all, happy growing, and best of luck to all competitors! See you all in the next update! Genetics - Runtz https://www.zamnesia.com/6000-zamnesia-seeds-runtz-feminized.html Nutrients - Plagron https://plagron.com/en/hobby - Aptus Holland https://aptus-holland.com/ Controls - Trol Master https://www.trolmaster.eu/ LED - https://www.futureofgrow.com/en LED - https://www.thinkgrowled.com Soil - https://www.promixgardening.com/en Germination - Cannakan https://cannakan.com/?srsltid=AfmBOopXr-inLXajXu3QFgKXCXXos4F1oEvScjMKIB5MR5dk8-GJ-F49 DOGDOCTOR 15% off Smoking Papers - https://ziggioriginal.com/ Terpene saver - https://grovebags.com/ As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciloved and i fell honored with you all in my life With true love comes happiness Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so As always, this is shared for educational purposes, aiming to spread understanding and appreciation for this plant. The journey with nature is one of discovery, creativity, and respect. Let’s celebrate it responsibly and continue to learn and grow together! Growers Love To you All 💚 #EternityGrowCup #RuntzHunt #GrowersLove #CannabisCommunity #AptusHolland #ProMixSoil #TrolMaster #Zamnesia #Plagron #ZiggiPapers #Grovebags

I’m not 100% done collecting all the data. By the smell of it, this plant will be one of the dankest plants I’ve ever grown. More to come. Tuesday, September 5 I have the nugs in jars right now. Hopefully I didn’t let it dry too much. Right now the humidity is at around 52% but I think if I move the jar around a bit I can them to sweat a bit more.

I develop an application that measures the THC levels of cannabis and the result comes from 3 photos of buds... Godfather OG measured relatively well, it also measured other strains such as Fat Banana Automatic, Gorilla Cookies Automatic, Northern Lights Automatic,White Widow Automatic and Gelato Auto... See how it works in the video THC Meter... ----------------------------------------------------- Day50 : ----------------------------------------------------- Day air temperature: 28c Night air temperature: 24c Air humidity: 46% No Watering ----------------------------------------------------- Day51 : ----------------------------------------------------- Day air temperature: 27c Night air temperature: 24c Air humidity: 39% PH : 6.4 Watering : 0.4 l Bio Bloom 1 ml / l ----------------------------------------------------- Day52 : ----------------------------------------------------- Day air temperature: 27c Night air temperature: 23c Air humidity: 43% No Watering ----------------------------------------------------- Day53 : ----------------------------------------------------- Day air temperature: 29c Night air temperature: 25c Air humidity: 39% PH : 6.4 Watering : 0.4 l Bio Bloom 1 ml / l ----------------------------------------------------- Day54 : ----------------------------------------------------- Day air temperature: 30c Night air temperature: 26c Air humidity: 45% PH : 6.5 Watering : 0.4 l Bio Bloom 1 ml / l ----------------------------------------------------- Day55 : ----------------------------------------------------- Day air temperature: 30c Night air temperature: 26c Air humidity: 38% PH : 6.4 Watering : 0.4 l Bio Bloom 1 ml / l ----------------------------------------------------- Day56 : ----------------------------------------------------- Day air temperature: 28c Night air temperature: 24c Air humidity: 40% Flushing Before Harvest

Finally got my blue dream from seedsman. Winner of the contest seeds of knowledge Here it is finally growing this beautiful lady I gotta say this one was almost broken, it arrived very well but since it was hidden as requested when I open the box with a razor to take out the seeds Packs my bad accidentally cut a tiny side of the seed Shell which I noticed and place into germination and now she is here with us and we are very happy to welcome her to our family Day 1 Tuesday Place into germination Fully at dark until next morning from 8AM Started to get light since she is a photoperiod At the end of the Day went and hid it in a box very well so its dark Day 2 Same process took her out and start again sunny Day for her indoors of course Day 3 Thursday finally got her showing its cotyledons doing the same process :3 And im guessing it goes with the sun At the end of the Day got to give her enough darkness she is a feminized seeds which I have to make her flower after idk some time so I will try and see how much she can grow and get STRESS for yield and potency, thanks a lot for your support Happy Friday btw 👽🌱 **** DAY 5 My girl is a monster and needs to recover Taproot was coming out of peatmoss and I didnt even notice so had to transplant to a Bigger pot soon, since she is not an auto she is way too fast cuz taproot was out in the bottom so I had to take the net out of the Peat moss so roots van expand comfortably but accidentally broke the tiny edge while sliding with lil to no pressure and it sucks but it was stuck and it was either that or just plant it like that but well probably I should have left her be but anyways she is young I think she will develop more since she is in a better soil mix now than just Peat moss, wish me luck cuz I really hope she is better and gets to grow huge, we will see at the process right This is whats all about Lets do our best tho We will! Enjoy & happy growing everyone ☺️

Day 52 - before pic/vid pulled em both out scrubbed tanks..... moved em into a different tent, with a different light.... did up completely different solutions, and currently still building it all.... Day 53 - still buildin... vids of new home... Nutrifield, Rock Nitro, 1200-1500 ppm and 6.5 PH.... see if we can give em a good boost.... left em in pure water before puttin em in new solution.... Day 57 - pics vid.... been adjusting ph... down to 5.8.... they are just growin along nicely in the new home... got a fan for the tent... looks good tho... solution temp is abit high, fan will fix that..... changed just the tanks to 5lt Cereal containers, they are alot taller, more room below the container... fits perfect.... Day 59 - pics vids, , 6.2ph, 1300ppm ... water temps been getting abit high, fans should arrive next week.... 👍😎 120W Aluminum+PC Plug 2835SMD 7995-9594LM 1131Pcs Red+234Pcs Blue * Blue(wavelength: 430nm, 554nm * Red(wavelength: 630nm 660nm)

mrjones Gorilla Cookies 🔹🔹🔹🔹🔹🔹WEEKLY GOALS🔹🔹🔹🔹🔹🔹 🌞Environment - 75/80℉ and 55% Humidity 💧 Feeding - Using "Super Coco" amended soil. compost teas, Dechlorinated H20 PH/6.5 Fish Fertilizer, about 4 ounces of water per day. 🍃Training / LST - Defoliation Compted last week, tonight finished up tieing down the side branches. 🕷️ IPM - Will be using Green Cleaner" 1 OZ per Gallon, and CannControl from Mammoth alternating between product each month for Integrated Pest Management. 💡240W QB288 V3 LM301H 4000k 660nm Red 🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹 Rambling - Moving into Week 10 here, and all these girls are doing so much better. Starting to stack on the bud weight. 🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹 ▶️Saturday 01.23.20 / Watered w/ Dechlorinated H20 PH 5.8 / 6.2 ▶️Sunday 01.24.21 / Watering Dechlorinated H20 PH to 5.8 / 6.2 ▶️Monday 01.25.21 / Watering Dechlorinated H20 PH to 5.8 / 6.2 ▶️Tuesday 01.26.21 / Watering Dechlorinated H20 PH to 5.8 / 6.2 ▶️Wednesday 01.27.21 / Did a Trichome check today and they are starting to get cloudy - Watering Dechlorinated H20 PH to 5.8 / 6.2 ▶️Thursday 01.28.21 / Watering Dechlorinated H20 PH to 5.8 / 6.2 - still packing on that bud weight! ▶️Friday 01.29.21 / Watering Dechlorinated H20 PH to 5.8 / 6.2 🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹 SOIL MIX COCO - 70/30% Tupur Royal Gold 30 GALLON WORM CASTINGS 5 GALLON LOBSTER COMPOST 5 GALLON PERLITE 6 GALLON INSECT FRASS 2 CUP ORGANICALLY DONE GROW 5 CUPS BUILD A SOIL COCO MINERAL MIX 6 CUPS

On day 3 -4 of 5 seeds have germinated! On day 4 all 4 seedlings were fed 500 ppm veg nutes. On day 5 seedlings were transplanted into 4" nursery pots with myco. I watered them in with 600 ppm of feed. Ppfd 450-500.

🌿 LA Punch by The Cali Connection 🌿 Week 7 Flower-day 2 16/10/2025 Nutrient change Floramax veg 1: 1L Floramax flowering enhancer: 400ml Floramax organabud:400ml Floramax rezin: 100ml ( around 1/4 dose) Floramax cal mag: 100ml Gp3 pro cal: 100ml ( unfortunately ran out and won't have any until next week ) Gp3 massive bloom: 100ml Cyco platinum series silica: 200ml Sleeps with the fishez: 400ml ( half dose system shock testing to see of this minimises/prevents problems per nutrients change like i was having ) Ph 5.9-6 - EC 2.2-2.3 ( Floramax wants e.c of 2.4, so I'm doing .1-.2 under for now to see how she responds LST and Defoilation is finally done on her, LA punch might get another LST tweak and a couple more Defoils. Unsure yet she's staying very tight so its a little harder to gauge how she'll respond I thought I should add, with the way I lst, I like to get as much defoilation out the way before flipping to flower that way all she does in flower is just get fat and lovely. Seemed to work well coming from only doing Autoflowers so just using that knowledge and implementing to my photo grows and tweaking it

Today marks a significant milestone in the journey of my cannabis plants as I have successfully set up the irrigation system. This new development will ensure an efficient and consistent supply of water for the plants, fostering a conducive environment for their growth. Up until now, I've been diligently top feeding the plants since their germination, laying a solid foundation for their root system. As a result, I anticipate that the plants will be well-equipped to adapt to this change and will continue to flourish. The plants have been thriving spectacularly and have demonstrated a clear preference for being dispersed over a larger surface area. I attribute this positive response to the method of main lining, a technique that has proven to be one of the most straightforward and effective approaches I've tried. The anticipation of seeing the final canopy and colas is immense, and I am eager to see how this technique will enhance the overall yield of my cannabis plants.

Week 4 of the flowering phase has arrived, and last week flew by 🌬️🌱. The ladies are thriving, and the buds are now clearly visible! ✨ The flowers are starting to turn darker, which is an exciting sign 🍒. The first trichomes are already forming, hinting at a very potent strain 💎🔥. While the cherry aroma hasn’t come through yet, removing some leaves revealed an intense, waxy scent – very intriguing! 🌿👃 I’ll keep you updated with daily posts. Drop a like and stay tuned to see how the ladies develop 🌺📸. See you next week! Woche 4 der Blütephase ist da, und die letzte Woche ist wirklich wie im Flug vergangen 🌬️🌱. Die Ladies haben sich prächtig entwickelt, und jetzt kann man die Buds schon richtig gut erkennen! ✨ Die Blüten beginnen langsam, sich dunkel zu verfärben – ein vielversprechendes Zeichen 🍒. Auch die ersten Trichome sind schon sichtbar, was definitiv auf einen sehr potenten Strain hindeutet 💎🔥. Das Kirscharoma ist zwar noch nicht zu erkennen, aber beim Entfernen der Blätter wurde ein intensiver, wachsähnlicher Duft wahrgenommen – super spannend! 🌿👃 Ich halte euch weiterhin mit täglichen Updates auf dem Laufenden. Lasst ein Like da und bleibt dran, um zu sehen, wie sich die Mädels entwickeln 🌺📸. Bis nächste Woche!

day 49 just pure water phed down. it is day 51, morning before lights on, I might chop today. I use a zero water filter pitcher got off walmart. My humidifier is only a sonic wave. If you use regular tap water it will get gunky in a sonic humidifier. If you have a heat humidifier that boils the water the mist should be clean. These are the 2 types of water humidifiers. Researching best water filters this "zero water" also comes with a free ppm meter! yay! Other water pitcher brands; the water still has ppm. So water misted will still have ppm contamination. You can easily used distilled water only, and reverse osmosis. I just fill a 4gal office water jug with the pitcher and fill my humidifier when needed. I do not use a humidifier in my entire grow up to this point when I chop, I need the perfect rh or this whole effort can fail. growing up to this point only requires 20% effort, chopping now for the 2 weeks dry is this 80% of crucial determination, succeed. A lot of tasks are required today for a clean chop. I feel confident, observed my cross seeds with pink paradise and one with a slightly open caylex looks dark enough for successful germination. I figured 8-16 seeds may be created, 3-4 buds were potentially pollinated. Many growers don't understand when creating genetics with female/female there is a slight chance of herm. You lose 50% of your genetic function. Just water last 2 days, no humic no folvic, 1st time entire grow ha! There is a lot of cleaning to do, remove the trees, clean the entire tent and environment with bleach water, hacksaw. I am very proud of my grow. I have sampled 3 of the strains, dr. thunder legit made my lips numb but it could have just been the terps removing my skin cells from the sticky filter, no lie. This is my best grow to date. I did this for you, and to document hype or not under the best conditions I could give my plants, full max potential, although I wish I had more lights, I am not looking for industrial lol, this is how I grow. Learn a lot on youtube and podcasts there is many good content and I may dab into it. You can have a room full of growers and you have 1 seed in your hand, they will all tell you that their way is correct ;) and the best way. Oreo is stinky AF, Bridal is just amazing glad its massive, just not into sativa but the test seemed slight sativa with majority indica, pink untested, dr, thunder just a lip numming plant aha, its very nice, all 4 amazing appeal like advertised, and excited for next run. Either crosses from this or other copycat genetics. I will give you beautiful glamout shots today, the sizes of the plants, My guesstimate bridal 1lb, thunder 1lb, pink, 0.4lb, bridal 0.5, I am ambitious off 640w lm301h, and 4x 20w blurples daisy chained. day 51 4 chopped see you in a few weeks :D

I soaked the seeds in distilled water for 18hours they were put into paper towels and baggies this morning as soon as tap root appears I will start photographing and posting diary of entire grow will be using 2 thirty gallons no till entirely build a soil both pots are on 3rd cycles both have red wiggler worms I am excited about these two strains and the 30 gallon po

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!