Recommended
Likes
55
Share
Ladies and gentlemen we are on day 82 and this girl is no more, through the loophole I can't find one clear trichome I understand the white hairs but I also understand the trichomes is what we go by.. also wanted to get her down because I was worried of possible root issues and did not want to ruin such a beautiful little plant.. Divine seeds has some great genetics this thing was topped and in such a small cup handled the training well and rewarded me with what I'm assuming is going to be between 28 and 35 grams so I'm thinking over a gram per ounce of dirt that's not too shabby.. I hope everyone in the contest is doing well and I hope everyone else is doing just as good.. we are all in this together, to be able to grow our own medicine at our own discretion and know what we're smoking is worth its weight in gold.. again big shout out to Divine Seeds for putting together this competition I thought I would do something a little different to stand out by doing a mini pot grow I don't know if it'll get me a trophy or not but it was sure fun growing it and was very beneficial to me in my personal development as a grower.. until next time folks and a month or so I will have the smoke report.. I tried to upload today for another strain and it wasn't working so hopefully grow diaries fixes the bug of not being able to update a smoke report.. happy growing everyone ✌️😎 ladies and gentlemen after 8 days of drying I trimmed her up today and as you can see from the video 46 g or 1.625 Oz which is extremely good considering the small amount of medium... Now I know during the cure I will probably lose around 1/8 of my total weight but still I will be definitely over 40 grams my guess will be at least 42 to 43 .. now for 28 Oz of soil I could not be happier.. between the genetics being great and the plants loving my feeding remedy all has been well.. I want to thank Divine seeds for throwing such an awesome contest and giving us enough time to do it right and also for providing the seeds but not just that they really hooked it up.. Big ups to them and I have more genetics that I will be running from them in the future.. hope everyone is doing well good luck to everyone in the contest and to everyone happy growing ✌️😎
Likes
25
Share
ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.
Processing
Likes
2
Share
@Nicogoyo
Follow
bueno en esta cuarta semana el olor se deja dominar ya dentro del espacio de cultivo cada día se le ajusta mas el lst a cada una para que los nuevos brazos con flor vallan recibiendo la misma luz que el resto de sus brazos mas grandes , llegamos al acuerdo de que el filtro de carbono es necesario así que instalaremos dentro de unos días mas para esconder olor dela plantación te de plátano para el crecimiento delas flores y engorde de flores durante su periodo de floración y misma dosis de riego cada 5 días j-12 dos dias para su 4 semana se apretan y ordenamos las ramas mejor para su efectividad S-14 ultimo.dia de su cuarta semana y cumpliendo un mes de vida y crecimiento ya bien acomododados y estructurados y floreciendo con fuerza mañana comenzamos la quinta semana esperamos unos dias para volver a amarrar ya es nevesario gias pa
Likes
7
Share
Beginn Blütewoche 7. Langsam kommt auch die Mimosa Gusher in Fahrt. Der Rest macht Sicht weiterhin gut. Auch wenn ich starke ph Schwankungen hatte.
Likes
7
Share
Hey everyone 🤗. There isn't much to report this week. It was topped again, otherwise everything is like last week. She is developing well 👍. I wish you lots of fun with the update, stay healthy and let it grow 🌱🍀 You can buy this Strain at https://thecaliconnection.com/original-sour-diesel.html You can buy this Nutrients at https://greenbuzzliquids.com/ Type: Original Sour Diesel ( Clone ) ☝️🏼 Genetics: Fem seeds- Original Sour Diesel to Original Sour Diesel BX3 RVSD Male Reg seeds- Original Sour Diesel x Original Sour Diesel BX3 Male 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 - 0.4 EC. Add Cal / Mag 2 ml per l water every 2 waterings . Ph with Organic Ph - Pulver to 5.8 .
Likes
7
Share
@Car1bGrow
Follow
The growth cycle was pretty short, realizing that I planted it late in the year with the sun setting way sooner. Overall great experience
Likes
10
Share
@Elpicor
Follow
Ph-ep pen broken, 1 week without ph properly adjustment ...................... plus the weather forecast says 2 weeks of rain, it will be a challenge to keep the humidity low in the house and of course in the growbox.
Likes
57
Share
It's time for a thrilling week 4 flower report on my magnificent Medusa F1 from Royal Queen Seeds. This girl is an absolute monster, and I'm not just talking about her size! She's bursting with buds that are starting to resemble baseballs scattered throughout the garden. It's like a home run derby in there! Now, let's talk about the power of mass defoliation. I carefully stripped away those excess leaves, creating space for the buds to shine. It's like giving them a VIP pass to the spotlight. By removing unnecessary foliage, we channel the plant's energy towards bud production. It's like a backstage makeover, where the stars of the show get all the attention. The result? Bigger, denser, and more potent buds that'll knock your socks off! I mean my Medusa's buds are growing so big that they're starting to look like baseballs. But hey, let's be grateful they're not golf balls, or else we'd have quite the game to play in the garden! I want to give a special shout out to Royal Queen Seeds for providing me with this incredible opportunity to grow the Medusa F1. Their genetics are truly exceptional, giving us the chance to cultivate plants that are nothing short of spectacular. Thank you, Royal Queen Seeds, for being the game-changers in the cannabis world! Stay tuned for more updates on the awe-inspiring journey with my Medusa F1. Until then, keep growing, keep smiling, and enjoy the amazing genetics that Royal Queen Seeds has to offer. Wishing you all bountiful harvests and an abundance of green goodness! As always thank you all for stopping by and for supporting me on this journey, i am super passion about growing and fell blessed to have you all with me on this new journey <3 <3 <3 Genetics - RQS MEDUSA F1 Ligth - LUMATEK ZEUS 465 COMPACT PRO 
Food - APTUS HOLLAND 
 
All info and full product details can be find in can find @ https://www.royalqueenseeds.com 

https://aptus-holland.com/
 
https://autopot.co.uk/ 

https://lumatek-lighting.com/ With true love comes happiness <3<3<3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3<3<3 <3 <3 <3 Growers love to you all <3 <3 <3 Medusa F1 Medusa is a true F1 hybrid created from pure, inbred cannabis lines. She boasts uniform grow traits, mouthwatering aromas and flavours, and plenty of potency. If you're looking to bring stable, elongated plants into your room, tent, or garden, look no further. Mouthwatering Aromas, High Potency, and Mid-Size Plants Though she won't turn you to stone like the mythical goddess after which she's named, Medusa F1 is bound to get you plenty stoned in her own special way. Combining genetics from inbred lines deriving from Sugar Magnolia, a thick and sweet indica, and American Beauty, a fast, fruity hybrid loved for its well-balanced, positive high, the result is an autoflowering F1 variety that produces fresh flavours, a unique cannabinoid profile, and large yields. A very aromatic cultivar with a vibrant concentration of terpenes bearing notes of fresh mint alongside an upfront peppery kick, backed up by hints of fresh fruit, berries, and tangy fuel. Her thick, frosty flowers consistently produce high levels of THC and CBG, as well as high concentrations of myrcene, ocimene, farnesene, and caryophyllene. As a result, Medusa F1 has strong effects that relax and stone the body from head to toe (brought on by particularly high concentrations of myrcene and farnesene) while motivating the mind with an uplifting, creative, and motivating kick (thanks to high concentrations of ocimene). Medusa F1 seeds produce elongated autoflowering plants with long, strong branches and well-spaced internodes. Plants regularly grow up to 80–85cm tall and boast a fast flowering time of just 42–45 days (72 days from germination to harvest). Thanks to her genetics, Medusa F1 produces plenty of thick flowers with big, swollen calyxes that make for very easy trimming. Medusa F1 is very stable, handling stress and unfavourable growing conditions with ease, though she is somewhat susceptible to Botrytis, so make sure to keep tabs on the humidity in your grow room/garden
Likes
3
Share
@Takeaims
Follow
Going clean my tent out and spruce things up a little going start new diary with new xxl and f1hybrids keeep and eye
Likes
6
Share
@Lomba610
Follow
is getting bigger and bigger, I made some toppings and continued with lst, out of curiosity I measured it, it occupies a space of 60x60cm impressive how fast it grows, this week I raised the ec to 2000, I think next week the I move 80x80cm to another box and make it bloom 🤩💪🏼
Likes
1
Share
Trichromes cloudy with a touch of amber. The smell is amazing similar to tropical fruits very sweet smell.
Likes
12
Share
@Krissci
Follow
Entering 2nd week of flowering... Mainlining for the 1st time. Largest plants of all Each plant has 8x nodes. Topped 3 times. Tied down to create an X style frame. 2x on this run 2x seedmans strawberry Banana grape in 5gal pots (1m2 tent) to compare. But strawberry Banana as a strain seems to be a big grower
Likes
3
Share
Checked the fast bud Cherry Cola and gorilla cookies in paper towel and it had a nice tap root so it went in to the the Ocean from Fox farm with the center using a starter seed CoCo mix. So now we have two seed planted with tap roots in soil with clear solo cup over the top with some spritzes of water in the cup to keep the humidity up over the seed until they pop through the soil.
Likes
4
Share
@UKBUDBROS
Follow
Popped all 8 seeds from 420 fastbuds and put them into half a pint of water that had been stood for 24 hrs at room temperature. left them float in a dark cupboard, 2 days later all of them sat at the bottom of the glass with big tap roots so I planted them in their final 15L pots.
Likes
26
Share
@XxxAuto
Follow
I’ll try them next again, then I should remember the net for trying a Scrog. It’s a old classic strain that no one forget ;-) if you not have try the white widow, then you must go on !
Likes
18
Share
10/22: wow… what a mess. I should have defoliated last week. They were incredibly overgrown and now i had to stress the hell out of them to get the tent cleared up for light. Hopefully the stress does not cause any issues. As if the defoliation was bot enough stress on them, my light height has reached its top, and PL1 had two colas starting that were already pressed into the light. And so i removed their tops a node or two down. Im sure this was as good as hitting the plant in the face with a brick. Hope she recovers! If youve been following along, you know i planted these with hopes for the extremely purple pheno. And i believe that if i had it, she would have started showing her colors by now. Looking forward to seeing what these turn into and if they make it without any issues after i kicked them around a bit.