With this 405g of only flowers i will made bubble hash and Show you the result. Maybe i do cold melt curing …

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Start week 7

Smelled amazing and was easy to grow. 1st Grow!

This week has been wild. I had to cut the sides of the greenhouse as the plants had it bursting at the seams. These girls are all flowering beautifully and starting to frost up. Happy Growing.

Bloom week 2 is going exactly as planned. The SCROG is now fully filled with a very flat and even canopy, covering the entire 4×4 grid. I finished the last structural work by lollipopping the plant completely from below and stopped all training. From this point on she can stretch freely and focus all her energy on flower development. Bud sites are well defined across the whole canopy and the plant looks healthy and balanced. Feeding is dialed in with stable pH and moderate EC, no signs of over- or underfeeding. Light intensity was increased gradually and the plant is responding very well. Overall a very clean transition from stretch into early flower, setting the stage for dense buds in the coming weeks.

Moving along; first topping came on day 18. Will include all details once done with topping (going for mainline). Photos taken 21 days after breaking soil

il ramo secondario piu esterno è anche quello piu cresciuto, è gia quasi al limite di altezza non so se continua cosi dovrò finire per topparlo o rischio che sia troppo vicino alla lampada ma se cosi fosse, quando sarebbe l'ideale topparlo? siamo gia in fioritura stresserei la pianta e rallenterebbe lo sviluppo, non so

The permanent markers are doing alright. They're still really short compared to the others, but they're finally starting to take off. I can kinda see them catching up soon. The new year is off to a frosty start! We've had snow falling for what feels like forever. My kids are loving it, of course. They're building forts, having snowball fights, and turning everything into a winter wonderland. It's been a lot of fun to watch them enjoy it. All this snow is really making me think about how quickly time flies. It feels like just yesterday we were celebrating the holidays, and now here we are knee-deep in winter. I'm looking forward to warmer weather, but for now, I'm enjoying the quiet beauty of fresh snow.

Hello growmies! 25/08 - Alimentação Royal Gorilla # 1 : CE = 1,39; pH = 6,3 O gráfico representa a nutrição da RG#1 misturada com 2,5L de água. 25/08 - Alimentação Royal Gorilla # 2 (2,5L): CE = 1,33; pH = 6,1 -BioGrow - 3ml; BioBloom - 5,5ml; TopMax - 2,5ml; BioHeaven - 6ml; Activera - 5ml; 25/08 - Alimentação Queijo Royal # 1 (2L): CE = 1,36; pH = 6,2 -BioGrow - 3ml; BioBloom - 6ml; TopMax - 2ml; BioHeaven - 6ml; Activera - 6ml; 21/08 - Alimentação Queijo Royal # 2 (2L) - CE = 1,54; pH = 6,3 -BioGrow - 3ml; BioBloom - 5ml; TopMax - 2ml; BioHeaven - 6ml; Activera - 6ml;

Lot of defoliation must have plucked 400+ leaves

Great week. Girls finishing up and taking longer to drink. Checked trichomes and this plant is ready with mostly cloudy and a spattering of amber. Will harvest tomorrow morning and will provide weights and measures after harvest. Will dry in tent without light and offset circulation. Will keep tent naturally at 67 degrees. I will need to assist with humidifier to get humidity to around 60 RH. Perhaps a wicking cloth in a bucket? Just thinking out options. Update: Cut down the girls and hang them up to dry. Great thick hard buds. Can't wait to see what they smoke like, but it is going to take time to dry. Thanks to everyone that helped to make this a successful harvest. I will weigh the harvest before starting cure after a closer trim.

Hi everyone 🤗 This week the Orange Sherbert Phenotype # 1 and the Zkittlez Phenotype # 2 were harvested :-) Now the Victory Kush 1 - 3, Zkittlez 1 - 2 and Orange Sherbert 1 are ready to dry. All others except the gelato are rinsing 👍 Orange Sherbert 2 will be harvested next week :-) Until then, I wish everyone a nice weekend, stay healthy and let it grow 🍀🌱👌

Not the best of yielding strains but the smoke definitely makes up for it, Happy Growing 😁

Fue una semana tranquila, viendo como poco a poco siguen creciendo y engordando esas flores, nevándose de a poco más y más gracias a Top Candy de Top Crop. Se cortaron las últimas hojas para despejar por completo su parte inferior y fuese aún más fácil el riego. Se sigue ... Va quedando menos ... 👽👽👽

THE END OF Grow Phase. START OF Bloom

Cheiro muito forte de Pinho e baunilha. Com certeza é o melhor resultado que já obtive cultivando com o coco.